Citrobacter phage SH3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; Citrobacter virus SH3

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172JGC6|A0A172JGC6_9CAUD Portal protein OS=Citrobacter phage SH3 OX=1805466 GN=sh3_0032 PE=3 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.17YY9 pKa = 10.19EE10 pKa = 4.4LLAATVKK17 pKa = 10.72AFNEE21 pKa = 4.43RR22 pKa = 11.84IQYY25 pKa = 10.62DD26 pKa = 4.93GIQEE30 pKa = 4.42DD31 pKa = 4.67DD32 pKa = 4.61DD33 pKa = 5.18YY34 pKa = 11.99SDD36 pKa = 4.3ALHH39 pKa = 6.43EE40 pKa = 4.55VVDD43 pKa = 4.29SQVPHH48 pKa = 6.66YY49 pKa = 9.07YY50 pKa = 10.35HH51 pKa = 7.68EE52 pKa = 4.81IFTVMAADD60 pKa = 5.4GIDD63 pKa = 3.84HH64 pKa = 6.56EE65 pKa = 5.23FEE67 pKa = 5.43DD68 pKa = 4.71SGLMPDD74 pKa = 3.68TKK76 pKa = 10.9DD77 pKa = 3.13VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.05ALYY91 pKa = 10.8NDD93 pKa = 3.95VSNSSDD99 pKa = 3.68VVWFEE104 pKa = 4.27VEE106 pKa = 3.97EE107 pKa = 4.23SDD109 pKa = 4.58EE110 pKa = 4.41AGEE113 pKa = 4.18YY114 pKa = 9.24WVVDD118 pKa = 3.78AKK120 pKa = 10.5TGIFVEE126 pKa = 4.48QAVSLDD132 pKa = 3.39VATACAKK139 pKa = 10.58DD140 pKa = 3.41NYY142 pKa = 10.91AIGRR146 pKa = 11.84HH147 pKa = 5.47LKK149 pKa = 10.62VEE151 pKa = 4.88DD152 pKa = 3.52INDD155 pKa = 3.43NVVFDD160 pKa = 4.29PAAAEE165 pKa = 4.13EE166 pKa = 4.35DD167 pKa = 4.35CEE169 pKa = 4.21

Molecular weight: 19.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172JGC3|A0A172JGC3_9CAUD Endopeptidase Rz OS=Citrobacter phage SH3 OX=1805466 GN=sh3_0044 PE=4 SV=1
MM1 pKa = 7.21QNTPRR6 pKa = 11.84TLRR9 pKa = 11.84HH10 pKa = 5.67RR11 pKa = 11.84RR12 pKa = 11.84IQGLLSKK19 pKa = 10.55LKK21 pKa = 9.35STKK24 pKa = 10.06CPNGFRR30 pKa = 11.84TKK32 pKa = 10.36CPRR35 pKa = 11.84WKK37 pKa = 10.44AALIGLLLTFSLTISGCASEE57 pKa = 4.7SNQRR61 pKa = 11.84VEE63 pKa = 4.06PRR65 pKa = 11.84KK66 pKa = 8.69VTVDD70 pKa = 3.15ASLMVTPNLTNEE82 pKa = 4.22MLNVLSPSDD91 pKa = 3.31

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12400

34

1295

253.1

28.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.895 ± 0.416

0.952 ± 0.165

6.403 ± 0.252

6.677 ± 0.383

3.556 ± 0.165

7.847 ± 0.387

1.798 ± 0.196

4.758 ± 0.15

6.5 ± 0.427

8.081 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.04 ± 0.138

4.371 ± 0.24

3.815 ± 0.216

4.016 ± 0.331

5.323 ± 0.234

6.056 ± 0.26

5.839 ± 0.319

7.089 ± 0.279

1.508 ± 0.18

3.476 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski