Lactococcus phage 1358

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Whiteheadvirus; Lactococcus virus 1358

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3W0G9|D3W0G9_9CAUD Uncharacterized protein OS=Lactococcus phage 1358 OX=741942 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 9.96AIHH5 pKa = 6.91HH6 pKa = 6.47IGIDD10 pKa = 3.11ITKK13 pKa = 10.04PEE15 pKa = 4.08TTGRR19 pKa = 11.84AEE21 pKa = 3.68SSEE24 pKa = 4.37YY25 pKa = 10.9VFAAYY30 pKa = 9.92DD31 pKa = 3.44VDD33 pKa = 4.01GEE35 pKa = 4.55EE36 pKa = 3.82ILEE39 pKa = 3.96YY40 pKa = 10.52RR41 pKa = 11.84GAEE44 pKa = 3.78VAMILTDD51 pKa = 4.29LATSILNGMYY61 pKa = 9.95CEE63 pKa = 4.07AMGYY67 pKa = 10.18DD68 pKa = 4.07YY69 pKa = 11.14EE70 pKa = 4.64PSEE73 pKa = 4.56GDD75 pKa = 3.02

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3W0F6|D3W0F6_9CAUD Uncharacterized protein OS=Lactococcus phage 1358 OX=741942 PE=4 SV=1
MM1 pKa = 7.7SMTDD5 pKa = 3.26EE6 pKa = 4.01QVARR10 pKa = 11.84LVEE13 pKa = 4.29IKK15 pKa = 10.49AGYY18 pKa = 8.53FARR21 pKa = 11.84KK22 pKa = 9.17RR23 pKa = 11.84MRR25 pKa = 11.84RR26 pKa = 11.84VIKK29 pKa = 10.52SAAAYY34 pKa = 9.42HH35 pKa = 6.64AFNKK39 pKa = 8.11NTVVKK44 pKa = 10.8VRR46 pKa = 11.84FAKK49 pKa = 10.02SAQVGRR55 pKa = 11.84TKK57 pKa = 9.79TVCFDD62 pKa = 4.0VIGKK66 pKa = 8.89NAISGQLAAQVVLGLDD82 pKa = 4.17FKK84 pKa = 10.73PTTKK88 pKa = 10.13KK89 pKa = 10.41RR90 pKa = 11.84AA91 pKa = 3.34

Molecular weight: 10.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

11482

42

849

267.0

29.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.754 ± 0.383

0.732 ± 0.119

7.194 ± 0.399

6.758 ± 0.584

3.684 ± 0.233

7.281 ± 0.386

1.803 ± 0.152

6.062 ± 0.332

7.203 ± 0.366

7.142 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.199

4.799 ± 0.322

3.397 ± 0.207

3.823 ± 0.256

4.808 ± 0.347

5.13 ± 0.283

6.628 ± 0.567

6.549 ± 0.268

1.263 ± 0.104

3.789 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski