Hubei sobemo-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KES8|A0A1L3KES8_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 9 OX=1923242 PE=4 SV=1
MM1 pKa = 8.23DD2 pKa = 4.41STPGNCQFKK11 pKa = 11.09SLGSTNKK18 pKa = 10.61DD19 pKa = 2.62IFKK22 pKa = 10.66YY23 pKa = 10.06EE24 pKa = 4.1AGICDD29 pKa = 4.17PEE31 pKa = 4.06RR32 pKa = 11.84VAMVRR37 pKa = 11.84CAVKK41 pKa = 10.6SRR43 pKa = 11.84LDD45 pKa = 3.55RR46 pKa = 11.84LIAGYY51 pKa = 10.69LEE53 pKa = 5.26ADD55 pKa = 4.15TINVFVKK62 pKa = 10.17PEE64 pKa = 3.73PHH66 pKa = 5.17TTAKK70 pKa = 9.33LTEE73 pKa = 4.03GRR75 pKa = 11.84LRR77 pKa = 11.84LISAVSLVDD86 pKa = 3.22TMIDD90 pKa = 2.76RR91 pKa = 11.84VLFGWVQRR99 pKa = 11.84KK100 pKa = 8.94ALTVVGRR107 pKa = 11.84TPCLCGWSPMVGGWRR122 pKa = 11.84YY123 pKa = 9.48IYY125 pKa = 10.84GRR127 pKa = 11.84FRR129 pKa = 11.84NQPVLCLDD137 pKa = 3.36KK138 pKa = 11.37SSWDD142 pKa = 3.27WTVQEE147 pKa = 4.72HH148 pKa = 6.56VVIFWKK154 pKa = 10.63RR155 pKa = 11.84FLSEE159 pKa = 3.76LAVGHH164 pKa = 6.0RR165 pKa = 11.84NWWTIAASARR175 pKa = 11.84FEE177 pKa = 4.38VLFKK181 pKa = 10.85LASFQFKK188 pKa = 10.59DD189 pKa = 3.46GTVVQQEE196 pKa = 4.32GTGIMKK202 pKa = 10.2SGCLLTLILNSVGQSYY218 pKa = 9.0LHH220 pKa = 5.94YY221 pKa = 10.82LSMLRR226 pKa = 11.84IGRR229 pKa = 11.84NPLQHH234 pKa = 6.51QPICVGDD241 pKa = 3.84DD242 pKa = 3.41TVQTSFEE249 pKa = 4.81DD250 pKa = 3.05IRR252 pKa = 11.84EE253 pKa = 4.08YY254 pKa = 10.65VYY256 pKa = 11.25EE257 pKa = 4.06MEE259 pKa = 4.58KK260 pKa = 10.75LGAVVKK266 pKa = 10.19GVKK269 pKa = 8.96IRR271 pKa = 11.84NWVEE275 pKa = 3.5FCGFAFAKK283 pKa = 6.95EE284 pKa = 4.45TCVPAYY290 pKa = 7.63WQKK293 pKa = 11.13HH294 pKa = 4.35LFKK297 pKa = 10.83LQYY300 pKa = 10.3CKK302 pKa = 11.01LEE304 pKa = 4.75DD305 pKa = 3.7SLASYY310 pKa = 10.42QMIYY314 pKa = 10.98ANEE317 pKa = 3.78PVMYY321 pKa = 10.44DD322 pKa = 3.4LLYY325 pKa = 10.76RR326 pKa = 11.84LAMQVNPEE334 pKa = 4.04LVITPVEE341 pKa = 4.23AKK343 pKa = 10.7LIMNGG348 pKa = 3.3

Molecular weight: 39.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KES8|A0A1L3KES8_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 9 OX=1923242 PE=4 SV=1
MM1 pKa = 7.06VFSEE5 pKa = 3.9ILMWSISSFYY15 pKa = 10.5AASFSSVMLVVIYY28 pKa = 8.89WHH30 pKa = 5.69LRR32 pKa = 11.84YY33 pKa = 8.71WRR35 pKa = 11.84KK36 pKa = 10.26RR37 pKa = 11.84IMRR40 pKa = 11.84NIMHH44 pKa = 6.84MWNLAEE50 pKa = 4.41YY51 pKa = 8.48YY52 pKa = 10.84VLMEE56 pKa = 4.23EE57 pKa = 4.43VEE59 pKa = 4.3PVYY62 pKa = 10.6QEE64 pKa = 4.07PKK66 pKa = 10.08GVMMDD71 pKa = 4.09FLEE74 pKa = 4.3TTYY77 pKa = 11.0GYY79 pKa = 10.59VVSKK83 pKa = 11.14LPGNSNLEE91 pKa = 3.81LFAYY95 pKa = 10.16VILSALGLIIAVFLLKK111 pKa = 10.72AIVKK115 pKa = 9.99SLMKK119 pKa = 10.29LYY121 pKa = 11.31VKK123 pKa = 9.6MQVYY127 pKa = 10.58DD128 pKa = 3.75LDD130 pKa = 4.11GFSEE134 pKa = 4.38KK135 pKa = 10.5VRR137 pKa = 11.84VGSVLEE143 pKa = 4.36NSMQEE148 pKa = 3.81PAFQAEE154 pKa = 4.07VWVRR158 pKa = 11.84RR159 pKa = 11.84KK160 pKa = 10.44GPYY163 pKa = 8.91VKK165 pKa = 10.19SGQGFLTNFGFFTAYY180 pKa = 10.11HH181 pKa = 4.99VVEE184 pKa = 4.57DD185 pKa = 4.31VEE187 pKa = 5.32SVMLVKK193 pKa = 10.65GSNKK197 pKa = 9.52VEE199 pKa = 3.78ITPDD203 pKa = 3.35LFVQLEE209 pKa = 4.27GDD211 pKa = 3.61VALYY215 pKa = 11.33KK216 pKa = 8.42MTQQVACKK224 pKa = 10.19LGLSQSKK231 pKa = 9.73LYY233 pKa = 10.14PIEE236 pKa = 4.48TPHH239 pKa = 7.24KK240 pKa = 8.12AGLVVRR246 pKa = 11.84VHH248 pKa = 6.99AFGQRR253 pKa = 11.84TMGVLRR259 pKa = 11.84PDD261 pKa = 3.17EE262 pKa = 4.62GFGLVVYY269 pKa = 9.84EE270 pKa = 4.35GSTIKK275 pKa = 10.68GFSGAPYY282 pKa = 8.8HH283 pKa = 6.48VGRR286 pKa = 11.84MVYY289 pKa = 9.74GMHH292 pKa = 6.47MGGSTVNLGLAGAYY306 pKa = 10.6LHH308 pKa = 6.48MLAKK312 pKa = 10.66YY313 pKa = 9.46EE314 pKa = 5.14LEE316 pKa = 4.33DD317 pKa = 3.61TDD319 pKa = 6.66DD320 pKa = 4.12YY321 pKa = 11.63LQQQIMNEE329 pKa = 3.84GKK331 pKa = 9.51EE332 pKa = 4.45FVWQRR337 pKa = 11.84SPYY340 pKa = 10.4DD341 pKa = 3.59PDD343 pKa = 3.25EE344 pKa = 4.23ARR346 pKa = 11.84VRR348 pKa = 11.84IEE350 pKa = 3.5GKK352 pKa = 10.54YY353 pKa = 9.5YY354 pKa = 8.56QTDD357 pKa = 2.94MDD359 pKa = 4.63FIRR362 pKa = 11.84RR363 pKa = 11.84MEE365 pKa = 3.97QKK367 pKa = 10.7VKK369 pKa = 10.53GKK371 pKa = 9.1QVSYY375 pKa = 10.71KK376 pKa = 10.27RR377 pKa = 11.84PDD379 pKa = 3.54YY380 pKa = 11.09EE381 pKa = 4.17EE382 pKa = 4.32EE383 pKa = 4.1ALSEE387 pKa = 4.27VSDD390 pKa = 3.98EE391 pKa = 4.58SLPLAPRR398 pKa = 11.84GAMSYY403 pKa = 10.47PDD405 pKa = 3.74SKK407 pKa = 11.66NLLEE411 pKa = 4.63APAVNAGASGKK422 pKa = 10.53LEE424 pKa = 3.84GRR426 pKa = 11.84VGVPKK431 pKa = 10.94GILNHH436 pKa = 6.0LRR438 pKa = 11.84EE439 pKa = 4.35IDD441 pKa = 3.76LEE443 pKa = 4.24SRR445 pKa = 11.84EE446 pKa = 4.24ALVSYY451 pKa = 10.79LMDD454 pKa = 4.19GQPVTRR460 pKa = 11.84VQQNEE465 pKa = 4.1VSGSTRR471 pKa = 11.84KK472 pKa = 9.84NSRR475 pKa = 11.84NRR477 pKa = 11.84KK478 pKa = 8.9RR479 pKa = 11.84KK480 pKa = 9.36LKK482 pKa = 10.52KK483 pKa = 9.32QVQRR487 pKa = 11.84QVKK490 pKa = 9.26QSSNNVV496 pKa = 3.04

Molecular weight: 56.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

844

348

496

422.0

48.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.687 ± 0.216

1.422 ± 1.081

4.028 ± 0.175

6.635 ± 0.731

4.028 ± 0.354

7.227 ± 0.384

1.777 ± 0.033

4.147 ± 0.638

6.28 ± 0.331

9.716 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.147 ± 0.613

3.318 ± 0.097

3.791 ± 0.035

4.621 ± 0.193

5.569 ± 0.111

6.635 ± 0.731

3.791 ± 0.859

10.308 ± 0.513

1.896 ± 0.608

4.976 ± 0.593
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski