Amino acid dipepetide frequency for Marinobacter phage AS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.162AlaAla: 10.162 ± 1.775
1.025AlaCys: 1.025 ± 0.275
6.149AlaAsp: 6.149 ± 0.633
9.308AlaGlu: 9.308 ± 1.327
2.904AlaPhe: 2.904 ± 0.412
8.198AlaGly: 8.198 ± 1.413
1.025AlaHis: 1.025 ± 0.281
5.465AlaIle: 5.465 ± 0.654
5.38AlaLys: 5.38 ± 0.616
8.284AlaLeu: 8.284 ± 0.984
2.904AlaMet: 2.904 ± 0.546
3.672AlaAsn: 3.672 ± 0.425
3.587AlaPro: 3.587 ± 0.671
3.757AlaGln: 3.757 ± 0.648
6.49AlaArg: 6.49 ± 0.749
5.892AlaSer: 5.892 ± 0.76
6.063AlaThr: 6.063 ± 0.87
6.063AlaVal: 6.063 ± 0.711
1.964AlaTrp: 1.964 ± 0.401
1.879AlaTyr: 1.879 ± 0.335
0.0AlaXaa: 0.0 ± 0.0
Cys
0.256CysAla: 0.256 ± 0.153
0.0CysCys: 0.0 ± 0.0
0.427CysAsp: 0.427 ± 0.187
0.769CysGlu: 0.769 ± 0.257
0.427CysPhe: 0.427 ± 0.242
0.854CysGly: 0.854 ± 0.284
0.427CysHis: 0.427 ± 0.188
0.342CysIle: 0.342 ± 0.172
0.427CysLys: 0.427 ± 0.2
0.427CysLeu: 0.427 ± 0.208
0.171CysMet: 0.171 ± 0.126
0.256CysAsn: 0.256 ± 0.144
0.427CysPro: 0.427 ± 0.175
0.683CysGln: 0.683 ± 0.246
0.769CysArg: 0.769 ± 0.256
0.342CysSer: 0.342 ± 0.219
0.512CysThr: 0.512 ± 0.193
0.427CysVal: 0.427 ± 0.186
0.171CysTrp: 0.171 ± 0.119
0.256CysTyr: 0.256 ± 0.157
0.0CysXaa: 0.0 ± 0.0
Asp
5.465AspAla: 5.465 ± 0.577
0.939AspCys: 0.939 ± 0.334
3.501AspAsp: 3.501 ± 0.579
3.587AspGlu: 3.587 ± 0.595
2.135AspPhe: 2.135 ± 0.395
4.953AspGly: 4.953 ± 0.794
1.366AspHis: 1.366 ± 0.44
2.904AspIle: 2.904 ± 0.422
2.477AspLys: 2.477 ± 0.457
5.978AspLeu: 5.978 ± 0.663
1.025AspMet: 1.025 ± 0.325
1.537AspAsn: 1.537 ± 0.271
2.904AspPro: 2.904 ± 0.546
4.355AspGln: 4.355 ± 0.601
4.526AspArg: 4.526 ± 0.611
3.672AspSer: 3.672 ± 0.514
4.014AspThr: 4.014 ± 0.603
3.672AspVal: 3.672 ± 0.439
1.281AspTrp: 1.281 ± 0.31
1.793AspTyr: 1.793 ± 0.466
0.0AspXaa: 0.0 ± 0.0
Glu
8.027GluAla: 8.027 ± 1.349
0.342GluCys: 0.342 ± 0.176
3.672GluAsp: 3.672 ± 0.59
4.099GluGlu: 4.099 ± 0.864
2.818GluPhe: 2.818 ± 0.547
3.587GluGly: 3.587 ± 0.624
1.196GluHis: 1.196 ± 0.348
4.526GluIle: 4.526 ± 0.658
3.757GluLys: 3.757 ± 0.835
6.917GluLeu: 6.917 ± 0.953
1.196GluMet: 1.196 ± 0.335
2.05GluAsn: 2.05 ± 0.406
2.989GluPro: 2.989 ± 0.717
4.611GluGln: 4.611 ± 1.007
5.551GluArg: 5.551 ± 0.929
4.099GluSer: 4.099 ± 0.614
2.904GluThr: 2.904 ± 0.349
3.757GluVal: 3.757 ± 0.578
1.025GluTrp: 1.025 ± 0.292
1.879GluTyr: 1.879 ± 0.291
0.0GluXaa: 0.0 ± 0.0
Phe
3.16PheAla: 3.16 ± 0.402
0.171PheCys: 0.171 ± 0.117
1.964PheAsp: 1.964 ± 0.424
2.05PheGlu: 2.05 ± 0.423
1.11PhePhe: 1.11 ± 0.411
1.879PheGly: 1.879 ± 0.474
0.427PheHis: 0.427 ± 0.179
1.11PheIle: 1.11 ± 0.331
1.11PheLys: 1.11 ± 0.262
2.562PheLeu: 2.562 ± 0.587
0.342PheMet: 0.342 ± 0.188
1.708PheAsn: 1.708 ± 0.337
1.025PhePro: 1.025 ± 0.288
1.452PheGln: 1.452 ± 0.416
2.818PheArg: 2.818 ± 0.394
2.391PheSer: 2.391 ± 0.505
1.964PheThr: 1.964 ± 0.388
2.135PheVal: 2.135 ± 0.348
0.427PheTrp: 0.427 ± 0.174
0.598PheTyr: 0.598 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
5.636GlyAla: 5.636 ± 0.951
0.598GlyCys: 0.598 ± 0.23
3.501GlyAsp: 3.501 ± 0.635
4.611GlyGlu: 4.611 ± 0.976
2.391GlyPhe: 2.391 ± 0.302
5.892GlyGly: 5.892 ± 0.895
1.11GlyHis: 1.11 ± 0.364
2.647GlyIle: 2.647 ± 0.548
3.672GlyLys: 3.672 ± 0.616
7.515GlyLeu: 7.515 ± 1.211
1.025GlyMet: 1.025 ± 0.266
3.928GlyAsn: 3.928 ± 0.72
1.964GlyPro: 1.964 ± 0.433
2.306GlyGln: 2.306 ± 0.58
5.209GlyArg: 5.209 ± 0.903
5.978GlySer: 5.978 ± 0.735
6.063GlyThr: 6.063 ± 0.868
4.355GlyVal: 4.355 ± 0.536
1.537GlyTrp: 1.537 ± 0.432
2.135GlyTyr: 2.135 ± 0.471
0.0GlyXaa: 0.0 ± 0.0
His
1.366HisAla: 1.366 ± 0.371
0.427HisCys: 0.427 ± 0.219
1.025HisAsp: 1.025 ± 0.29
1.196HisGlu: 1.196 ± 0.418
0.598HisPhe: 0.598 ± 0.19
0.769HisGly: 0.769 ± 0.322
0.769HisHis: 0.769 ± 0.275
0.683HisIle: 0.683 ± 0.198
0.683HisLys: 0.683 ± 0.273
1.11HisLeu: 1.11 ± 0.311
0.427HisMet: 0.427 ± 0.229
0.427HisAsn: 0.427 ± 0.176
0.769HisPro: 0.769 ± 0.28
0.769HisGln: 0.769 ± 0.245
1.708HisArg: 1.708 ± 0.476
1.025HisSer: 1.025 ± 0.447
1.196HisThr: 1.196 ± 0.378
0.512HisVal: 0.512 ± 0.211
0.171HisTrp: 0.171 ± 0.129
0.512HisTyr: 0.512 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
4.697IleAla: 4.697 ± 0.938
0.598IleCys: 0.598 ± 0.25
4.355IleAsp: 4.355 ± 0.499
5.722IleGlu: 5.722 ± 0.645
1.537IlePhe: 1.537 ± 0.337
4.184IleGly: 4.184 ± 0.65
0.683IleHis: 0.683 ± 0.237
2.135IleIle: 2.135 ± 0.402
2.306IleLys: 2.306 ± 0.397
3.928IleLeu: 3.928 ± 0.514
1.281IleMet: 1.281 ± 0.356
2.306IleAsn: 2.306 ± 0.372
2.647IlePro: 2.647 ± 0.47
2.22IleGln: 2.22 ± 0.464
3.587IleArg: 3.587 ± 0.622
3.587IleSer: 3.587 ± 0.663
4.014IleThr: 4.014 ± 0.613
2.647IleVal: 2.647 ± 0.509
0.085IleTrp: 0.085 ± 0.091
1.623IleTyr: 1.623 ± 0.389
0.0IleXaa: 0.0 ± 0.0
Lys
6.49LysAla: 6.49 ± 0.92
0.256LysCys: 0.256 ± 0.177
3.245LysAsp: 3.245 ± 0.428
2.904LysGlu: 2.904 ± 0.438
1.537LysPhe: 1.537 ± 0.432
3.245LysGly: 3.245 ± 0.496
0.939LysHis: 0.939 ± 0.34
2.818LysIle: 2.818 ± 0.566
2.733LysLys: 2.733 ± 0.685
4.014LysLeu: 4.014 ± 0.647
0.427LysMet: 0.427 ± 0.213
0.598LysAsn: 0.598 ± 0.327
2.818LysPro: 2.818 ± 0.654
2.477LysGln: 2.477 ± 0.458
3.672LysArg: 3.672 ± 0.621
3.16LysSer: 3.16 ± 0.621
2.391LysThr: 2.391 ± 0.447
3.501LysVal: 3.501 ± 0.553
0.512LysTrp: 0.512 ± 0.205
0.939LysTyr: 0.939 ± 0.261
0.0LysXaa: 0.0 ± 0.0
Leu
9.052LeuAla: 9.052 ± 0.824
0.683LeuCys: 0.683 ± 0.275
6.234LeuAsp: 6.234 ± 0.668
6.917LeuGlu: 6.917 ± 0.917
2.135LeuPhe: 2.135 ± 0.418
4.782LeuGly: 4.782 ± 0.586
0.769LeuHis: 0.769 ± 0.282
5.38LeuIle: 5.38 ± 0.763
4.611LeuLys: 4.611 ± 0.704
5.807LeuLeu: 5.807 ± 0.686
1.366LeuMet: 1.366 ± 0.363
4.697LeuAsn: 4.697 ± 0.632
4.014LeuPro: 4.014 ± 0.742
3.928LeuGln: 3.928 ± 0.685
4.697LeuArg: 4.697 ± 0.598
5.722LeuSer: 5.722 ± 0.779
5.124LeuThr: 5.124 ± 0.936
5.551LeuVal: 5.551 ± 0.593
0.769LeuTrp: 0.769 ± 0.241
2.22LeuTyr: 2.22 ± 0.495
0.0LeuXaa: 0.0 ± 0.0
Met
2.733MetAla: 2.733 ± 0.547
0.0MetCys: 0.0 ± 0.0
1.196MetAsp: 1.196 ± 0.359
0.769MetGlu: 0.769 ± 0.276
0.598MetPhe: 0.598 ± 0.253
1.025MetGly: 1.025 ± 0.33
0.342MetHis: 0.342 ± 0.169
1.793MetIle: 1.793 ± 0.456
1.623MetLys: 1.623 ± 0.458
1.025MetLeu: 1.025 ± 0.346
0.427MetMet: 0.427 ± 0.204
0.683MetAsn: 0.683 ± 0.272
1.281MetPro: 1.281 ± 0.441
0.854MetGln: 0.854 ± 0.248
1.452MetArg: 1.452 ± 0.32
1.964MetSer: 1.964 ± 0.453
1.196MetThr: 1.196 ± 0.377
1.537MetVal: 1.537 ± 0.433
0.0MetTrp: 0.0 ± 0.0
0.085MetTyr: 0.085 ± 0.08
0.0MetXaa: 0.0 ± 0.0
Asn
2.989AsnAla: 2.989 ± 0.495
0.427AsnCys: 0.427 ± 0.228
2.391AsnAsp: 2.391 ± 0.503
2.477AsnGlu: 2.477 ± 0.434
1.025AsnPhe: 1.025 ± 0.229
2.135AsnGly: 2.135 ± 0.489
0.683AsnHis: 0.683 ± 0.272
0.939AsnIle: 0.939 ± 0.28
1.879AsnLys: 1.879 ± 0.411
4.099AsnLeu: 4.099 ± 0.627
1.366AsnMet: 1.366 ± 0.47
1.196AsnAsn: 1.196 ± 0.369
2.05AsnPro: 2.05 ± 0.426
2.647AsnGln: 2.647 ± 0.418
2.733AsnArg: 2.733 ± 0.446
2.135AsnSer: 2.135 ± 0.364
2.818AsnThr: 2.818 ± 0.582
2.05AsnVal: 2.05 ± 0.517
0.939AsnTrp: 0.939 ± 0.301
0.854AsnTyr: 0.854 ± 0.29
0.0AsnXaa: 0.0 ± 0.0
Pro
4.611ProAla: 4.611 ± 0.722
0.598ProCys: 0.598 ± 0.3
4.184ProAsp: 4.184 ± 0.927
3.843ProGlu: 3.843 ± 0.795
1.11ProPhe: 1.11 ± 0.279
3.245ProGly: 3.245 ± 0.688
1.025ProHis: 1.025 ± 0.404
3.16ProIle: 3.16 ± 0.648
1.537ProLys: 1.537 ± 0.422
3.16ProLeu: 3.16 ± 0.661
1.025ProMet: 1.025 ± 0.272
2.05ProAsn: 2.05 ± 0.412
2.22ProPro: 2.22 ± 0.711
1.452ProGln: 1.452 ± 0.419
2.818ProArg: 2.818 ± 0.548
2.562ProSer: 2.562 ± 0.401
2.647ProThr: 2.647 ± 0.503
2.989ProVal: 2.989 ± 0.669
1.11ProTrp: 1.11 ± 0.309
1.366ProTyr: 1.366 ± 0.338
0.0ProXaa: 0.0 ± 0.0
Gln
6.576GlnAla: 6.576 ± 0.742
0.342GlnCys: 0.342 ± 0.151
2.135GlnAsp: 2.135 ± 0.396
2.733GlnGlu: 2.733 ± 0.497
1.11GlnPhe: 1.11 ± 0.309
3.245GlnGly: 3.245 ± 0.45
1.196GlnHis: 1.196 ± 0.413
2.562GlnIle: 2.562 ± 0.502
2.477GlnLys: 2.477 ± 0.507
4.441GlnLeu: 4.441 ± 0.767
1.281GlnMet: 1.281 ± 0.341
1.196GlnAsn: 1.196 ± 0.326
2.647GlnPro: 2.647 ± 0.489
2.904GlnGln: 2.904 ± 0.671
2.989GlnArg: 2.989 ± 0.583
2.135GlnSer: 2.135 ± 0.513
3.16GlnThr: 3.16 ± 0.51
2.306GlnVal: 2.306 ± 0.426
1.025GlnTrp: 1.025 ± 0.316
1.281GlnTyr: 1.281 ± 0.313
0.0GlnXaa: 0.0 ± 0.0
Arg
6.832ArgAla: 6.832 ± 0.622
0.256ArgCys: 0.256 ± 0.159
4.184ArgAsp: 4.184 ± 0.475
5.295ArgGlu: 5.295 ± 0.757
2.05ArgPhe: 2.05 ± 0.436
4.953ArgGly: 4.953 ± 1.006
1.793ArgHis: 1.793 ± 0.423
3.587ArgIle: 3.587 ± 0.6
3.928ArgLys: 3.928 ± 0.609
7.088ArgLeu: 7.088 ± 0.887
1.708ArgMet: 1.708 ± 0.453
2.477ArgAsn: 2.477 ± 0.502
3.16ArgPro: 3.16 ± 0.739
4.611ArgGln: 4.611 ± 0.775
5.807ArgArg: 5.807 ± 0.838
3.757ArgSer: 3.757 ± 0.673
3.33ArgThr: 3.33 ± 0.564
4.099ArgVal: 4.099 ± 0.588
0.939ArgTrp: 0.939 ± 0.294
1.793ArgTyr: 1.793 ± 0.419
0.0ArgXaa: 0.0 ± 0.0
Ser
6.319SerAla: 6.319 ± 0.869
0.512SerCys: 0.512 ± 0.227
4.184SerAsp: 4.184 ± 0.493
3.074SerGlu: 3.074 ± 0.849
1.708SerPhe: 1.708 ± 0.355
5.209SerGly: 5.209 ± 0.561
0.342SerHis: 0.342 ± 0.183
3.757SerIle: 3.757 ± 0.595
2.904SerLys: 2.904 ± 0.642
5.465SerLeu: 5.465 ± 0.711
1.708SerMet: 1.708 ± 0.494
2.477SerAsn: 2.477 ± 0.398
2.904SerPro: 2.904 ± 0.545
2.647SerGln: 2.647 ± 0.528
5.465SerArg: 5.465 ± 0.789
4.441SerSer: 4.441 ± 1.05
3.074SerThr: 3.074 ± 0.602
4.611SerVal: 4.611 ± 0.581
1.11SerTrp: 1.11 ± 0.31
2.22SerTyr: 2.22 ± 0.442
0.0SerXaa: 0.0 ± 0.0
Thr
4.868ThrAla: 4.868 ± 0.731
0.256ThrCys: 0.256 ± 0.144
4.355ThrAsp: 4.355 ± 0.691
3.843ThrGlu: 3.843 ± 0.549
1.964ThrPhe: 1.964 ± 0.398
6.405ThrGly: 6.405 ± 1.089
0.683ThrHis: 0.683 ± 0.288
4.868ThrIle: 4.868 ± 0.724
2.477ThrLys: 2.477 ± 0.542
5.465ThrLeu: 5.465 ± 0.891
0.598ThrMet: 0.598 ± 0.233
2.22ThrAsn: 2.22 ± 0.428
3.245ThrPro: 3.245 ± 0.567
2.22ThrGln: 2.22 ± 0.466
3.16ThrArg: 3.16 ± 0.512
3.928ThrSer: 3.928 ± 0.7
3.416ThrThr: 3.416 ± 0.561
4.953ThrVal: 4.953 ± 0.819
1.025ThrTrp: 1.025 ± 0.291
1.452ThrTyr: 1.452 ± 0.318
0.0ThrXaa: 0.0 ± 0.0
Val
7.344ValAla: 7.344 ± 0.962
0.598ValCys: 0.598 ± 0.238
3.074ValAsp: 3.074 ± 0.363
4.014ValGlu: 4.014 ± 0.551
2.05ValPhe: 2.05 ± 0.405
3.928ValGly: 3.928 ± 0.733
0.598ValHis: 0.598 ± 0.21
3.501ValIle: 3.501 ± 0.503
2.562ValLys: 2.562 ± 0.36
3.757ValLeu: 3.757 ± 0.56
1.366ValMet: 1.366 ± 0.34
2.562ValAsn: 2.562 ± 0.564
3.672ValPro: 3.672 ± 0.602
2.05ValGln: 2.05 ± 0.395
4.782ValArg: 4.782 ± 0.866
4.611ValSer: 4.611 ± 0.519
5.209ValThr: 5.209 ± 0.859
5.295ValVal: 5.295 ± 0.688
0.769ValTrp: 0.769 ± 0.243
1.452ValTyr: 1.452 ± 0.381
0.0ValXaa: 0.0 ± 0.0
Trp
1.793TrpAla: 1.793 ± 0.443
0.171TrpCys: 0.171 ± 0.128
1.11TrpAsp: 1.11 ± 0.452
0.427TrpGlu: 0.427 ± 0.172
0.171TrpPhe: 0.171 ± 0.118
1.366TrpGly: 1.366 ± 0.312
0.171TrpHis: 0.171 ± 0.12
0.939TrpIle: 0.939 ± 0.26
1.025TrpLys: 1.025 ± 0.317
1.281TrpLeu: 1.281 ± 0.325
0.427TrpMet: 0.427 ± 0.206
0.512TrpAsn: 0.512 ± 0.182
0.854TrpPro: 0.854 ± 0.341
0.512TrpGln: 0.512 ± 0.207
1.196TrpArg: 1.196 ± 0.358
1.281TrpSer: 1.281 ± 0.292
0.939TrpThr: 0.939 ± 0.252
1.11TrpVal: 1.11 ± 0.314
0.342TrpTrp: 0.342 ± 0.185
0.171TrpTyr: 0.171 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.793TyrAla: 1.793 ± 0.43
0.171TyrCys: 0.171 ± 0.124
1.196TyrAsp: 1.196 ± 0.439
1.366TyrGlu: 1.366 ± 0.309
1.025TyrPhe: 1.025 ± 0.212
2.22TyrGly: 2.22 ± 0.349
0.598TyrHis: 0.598 ± 0.188
1.025TyrIle: 1.025 ± 0.267
1.11TyrLys: 1.11 ± 0.316
2.135TyrLeu: 2.135 ± 0.484
0.427TyrMet: 0.427 ± 0.18
1.281TyrAsn: 1.281 ± 0.361
1.537TyrPro: 1.537 ± 0.393
1.281TyrGln: 1.281 ± 0.342
2.22TyrArg: 2.22 ± 0.496
1.537TyrSer: 1.537 ± 0.362
1.366TyrThr: 1.366 ± 0.462
1.623TyrVal: 1.623 ± 0.396
0.598TyrTrp: 0.598 ± 0.205
0.939TyrTyr: 0.939 ± 0.313
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (11711 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski