Hubei tombus-like virus 27

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG61|A0A1L3KG61_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 27 OX=1923274 PE=4 SV=1
MM1 pKa = 7.5EE2 pKa = 5.56SIKK5 pKa = 10.98AFAQRR10 pKa = 11.84ISGWMMPKK18 pKa = 8.88KK19 pKa = 9.15TDD21 pKa = 3.78LQNQLDD27 pKa = 4.49TIKK30 pKa = 10.35YY31 pKa = 8.1IQMLLRR37 pKa = 11.84SKK39 pKa = 10.3PGQEE43 pKa = 4.18EE44 pKa = 3.76FHH46 pKa = 7.26DD47 pKa = 4.82AVDD50 pKa = 3.87NLNACKK56 pKa = 9.8TGPQIKK62 pKa = 8.21TVEE65 pKa = 4.41DD66 pKa = 4.04YY67 pKa = 11.64EE68 pKa = 4.8NDD70 pKa = 3.11ARR72 pKa = 11.84INAIRR77 pKa = 11.84SANQEE82 pKa = 3.58AALNHH87 pKa = 6.29LNYY90 pKa = 10.39KK91 pKa = 10.49SIGKK95 pKa = 9.75FIDD98 pKa = 3.35EE99 pKa = 5.85NYY101 pKa = 7.23TTWCAATLITYY112 pKa = 10.24LDD114 pKa = 3.66TVLITDD120 pKa = 3.31EE121 pKa = 4.46KK122 pKa = 10.51EE123 pKa = 3.93RR124 pKa = 11.84YY125 pKa = 9.75DD126 pKa = 4.26IISYY130 pKa = 8.71CIKK133 pKa = 10.21MNIAHH138 pKa = 6.41RR139 pKa = 11.84TANEE143 pKa = 3.73RR144 pKa = 11.84FIGEE148 pKa = 4.19NNFNDD153 pKa = 3.51IEE155 pKa = 4.15RR156 pKa = 11.84LNEE159 pKa = 3.81SSLTITRR166 pKa = 11.84DD167 pKa = 3.56KK168 pKa = 10.49PWWAFGFEE176 pKa = 4.22RR177 pKa = 11.84TRR179 pKa = 11.84GLTTKK184 pKa = 10.97ADD186 pKa = 3.91LNKK189 pKa = 10.11NRR191 pKa = 11.84ALVWCFRR198 pKa = 11.84PSTTSIIISTLGAIVIIDD216 pKa = 3.88KK217 pKa = 10.85LRR219 pKa = 11.84TIQQFKK225 pKa = 8.3TLRR228 pKa = 11.84NTITTLQAVNHH239 pKa = 6.27

Molecular weight: 27.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG61|A0A1L3KG61_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 27 OX=1923274 PE=4 SV=1
MM1 pKa = 7.7VFQTVNNEE9 pKa = 3.83HH10 pKa = 6.41NNINFRR16 pKa = 11.84SDD18 pKa = 2.8RR19 pKa = 11.84YY20 pKa = 9.8NRR22 pKa = 11.84QIAHH26 pKa = 6.16YY27 pKa = 8.94PAIQDD32 pKa = 3.54PAQYY36 pKa = 10.49YY37 pKa = 7.76YY38 pKa = 10.71YY39 pKa = 9.64PSSRR43 pKa = 11.84EE44 pKa = 3.87SLIAAIKK51 pKa = 9.84NRR53 pKa = 11.84HH54 pKa = 4.66EE55 pKa = 4.0PEE57 pKa = 3.81RR58 pKa = 11.84TKK60 pKa = 10.77LYY62 pKa = 10.11QPKK65 pKa = 9.28IQKK68 pKa = 10.13NIYY71 pKa = 7.84KK72 pKa = 10.04HH73 pKa = 4.77IRR75 pKa = 11.84KK76 pKa = 9.22FIMKK80 pKa = 7.54VQPWTRR86 pKa = 11.84QQYY89 pKa = 9.12VDD91 pKa = 5.36AIIDD95 pKa = 3.54PGKK98 pKa = 10.07RR99 pKa = 11.84KK100 pKa = 9.97SYY102 pKa = 10.53QDD104 pKa = 3.85CLDD107 pKa = 3.32EE108 pKa = 4.23MEE110 pKa = 4.58RR111 pKa = 11.84TGKK114 pKa = 10.26VYY116 pKa = 10.71SHH118 pKa = 7.21IVPHH122 pKa = 5.76TKK124 pKa = 9.25IEE126 pKa = 4.07KK127 pKa = 9.35MKK129 pKa = 10.0SNKK132 pKa = 9.44YY133 pKa = 9.04KK134 pKa = 10.68APRR137 pKa = 11.84LIQARR142 pKa = 11.84HH143 pKa = 4.15MTHH146 pKa = 6.35NIEE149 pKa = 3.97VGRR152 pKa = 11.84YY153 pKa = 7.78LKK155 pKa = 10.4PLEE158 pKa = 4.17IAVKK162 pKa = 10.41SKK164 pKa = 10.64FINFTKK170 pKa = 10.64GNYY173 pKa = 9.63DD174 pKa = 3.18SVAARR179 pKa = 11.84IEE181 pKa = 3.99KK182 pKa = 10.03LASKK186 pKa = 9.99FKK188 pKa = 10.85YY189 pKa = 8.81FTEE192 pKa = 4.35SDD194 pKa = 3.45HH195 pKa = 5.68VTFDD199 pKa = 3.04AHH201 pKa = 5.82ITVEE205 pKa = 4.21HH206 pKa = 6.72LKK208 pKa = 8.33MTHH211 pKa = 6.38KK212 pKa = 10.68FYY214 pKa = 10.85LACFNHH220 pKa = 6.91DD221 pKa = 3.3RR222 pKa = 11.84TLQKK226 pKa = 10.68YY227 pKa = 9.31LKK229 pKa = 8.96KK230 pKa = 9.36TINNRR235 pKa = 11.84IRR237 pKa = 11.84TRR239 pKa = 11.84DD240 pKa = 3.36GTKK243 pKa = 7.21WTVRR247 pKa = 11.84GTRR250 pKa = 11.84MSGDD254 pKa = 3.13VDD256 pKa = 3.61TSFGNSLINYY266 pKa = 7.82AIIMQVLEE274 pKa = 3.82NLQLKK279 pKa = 10.19GDD281 pKa = 4.67AIVNGDD287 pKa = 3.79DD288 pKa = 5.22SIIFTNQKK296 pKa = 9.67VDD298 pKa = 3.25NKK300 pKa = 9.89LAKK303 pKa = 10.27IMFDD307 pKa = 3.19IYY309 pKa = 11.14NQEE312 pKa = 4.1TEE314 pKa = 4.12IKK316 pKa = 9.42EE317 pKa = 4.33SEE319 pKa = 4.12NSIHH323 pKa = 6.86RR324 pKa = 11.84VEE326 pKa = 4.88FCRR329 pKa = 11.84TRR331 pKa = 11.84LVYY334 pKa = 9.55PASGKK339 pKa = 7.71PTMMMDD345 pKa = 4.2PKK347 pKa = 10.22RR348 pKa = 11.84LNSIYY353 pKa = 10.92GMTYY357 pKa = 10.61KK358 pKa = 10.88LIPPEE363 pKa = 4.28EE364 pKa = 4.06YY365 pKa = 10.78ANYY368 pKa = 10.26LINIKK373 pKa = 9.93KK374 pKa = 10.53ANEE377 pKa = 4.42SINKK381 pKa = 5.87NTPIGHH387 pKa = 6.05YY388 pKa = 8.15WHH390 pKa = 6.94TDD392 pKa = 3.05EE393 pKa = 5.52EE394 pKa = 4.86VKK396 pKa = 10.38HH397 pKa = 6.68DD398 pKa = 3.53KK399 pKa = 11.05HH400 pKa = 8.0IDD402 pKa = 3.45YY403 pKa = 10.66SLKK406 pKa = 10.47RR407 pKa = 11.84VITRR411 pKa = 11.84EE412 pKa = 4.19SVNQTSTTEE421 pKa = 3.46ITITMFIAYY430 pKa = 7.67PNLLEE435 pKa = 5.12DD436 pKa = 4.18LNKK439 pKa = 8.16TTEE442 pKa = 4.56LIKK445 pKa = 10.91RR446 pKa = 11.84NLPRR450 pKa = 11.84KK451 pKa = 9.66NSPSWFIDD459 pKa = 3.1HH460 pKa = 7.56DD461 pKa = 4.27NKK463 pKa = 10.74KK464 pKa = 9.95LHH466 pKa = 5.87QLNDD470 pKa = 3.26

Molecular weight: 55.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

709

239

470

354.5

41.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.501 ± 1.066

0.987 ± 0.36

5.642 ± 0.113

5.783 ± 0.039

3.526 ± 0.126

2.962 ± 0.202

3.385 ± 0.898

9.873 ± 0.528

8.886 ± 1.15

6.629 ± 0.693

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.309

7.757 ± 0.101

3.385 ± 0.679

3.949 ± 0.123

6.065 ± 0.111

5.078 ± 0.249

8.039 ± 1.051

3.808 ± 0.681

1.269 ± 0.432

4.795 ± 0.98
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski