Streptococcus satellite phage Javan200

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZGY5|A0A4D5ZGY5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan200 OX=2558563 GN=JavanS200_0011 PE=4 SV=1
MM1 pKa = 7.39LVTYY5 pKa = 8.27PALFYY10 pKa = 11.35YY11 pKa = 10.56DD12 pKa = 3.96DD13 pKa = 4.12TDD15 pKa = 3.96GASAPYY21 pKa = 9.78FVTFPDD27 pKa = 4.21FEE29 pKa = 4.62YY30 pKa = 10.92SATQGEE36 pKa = 4.64DD37 pKa = 2.95MAEE40 pKa = 3.86AMAMASEE47 pKa = 4.17WLGITLADD55 pKa = 3.95YY56 pKa = 10.83IEE58 pKa = 4.56NGRR61 pKa = 11.84DD62 pKa = 3.2IPVPSPINTLSLVDD76 pKa = 4.05NNPFRR81 pKa = 11.84DD82 pKa = 3.86DD83 pKa = 3.58KK84 pKa = 11.4DD85 pKa = 3.43IEE87 pKa = 4.32LVYY90 pKa = 10.8DD91 pKa = 4.01PSKK94 pKa = 11.29SFISMVMVDD103 pKa = 3.25VAEE106 pKa = 4.14YY107 pKa = 10.49LGSQEE112 pKa = 4.15PVKK115 pKa = 10.18KK116 pKa = 9.12TLTIPRR122 pKa = 11.84WADD125 pKa = 3.11TLGRR129 pKa = 11.84DD130 pKa = 3.39LGLNFSQTLTDD141 pKa = 5.46AIADD145 pKa = 4.09KK146 pKa = 10.69KK147 pKa = 10.83LHH149 pKa = 5.85AA150 pKa = 5.36

Molecular weight: 16.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZKI3|A0A4D5ZKI3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan200 OX=2558563 GN=JavanS200_0007 PE=4 SV=1
MM1 pKa = 6.69TTFTKK6 pKa = 10.37EE7 pKa = 3.67FQQRR11 pKa = 11.84LLEE14 pKa = 4.18QINGVLQEE22 pKa = 3.91RR23 pKa = 11.84TRR25 pKa = 11.84IEE27 pKa = 4.13DD28 pKa = 3.26EE29 pKa = 3.78RR30 pKa = 11.84QKK32 pKa = 11.14RR33 pKa = 11.84KK34 pKa = 8.29TEE36 pKa = 3.79ANRR39 pKa = 11.84KK40 pKa = 7.71YY41 pKa = 10.7GKK43 pKa = 10.09KK44 pKa = 10.07RR45 pKa = 11.84GG46 pKa = 3.61

Molecular weight: 5.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

2282

40

387

142.6

16.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.442 ± 0.679

0.745 ± 0.27

5.346 ± 0.624

8.545 ± 0.935

3.725 ± 0.433

4.996 ± 0.467

1.315 ± 0.306

6.223 ± 0.321

10.517 ± 0.586

9.86 ± 0.586

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.323 ± 0.35

6.004 ± 0.573

2.892 ± 0.398

3.988 ± 0.461

4.952 ± 0.654

4.382 ± 0.593

6.442 ± 0.584

4.952 ± 0.416

1.139 ± 0.149

5.215 ± 0.477
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski