Amino acid dipepetide frequency for Mycobacterium phage Pinto

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.864AlaAla: 12.864 ± 1.329
0.565AlaCys: 0.565 ± 0.156
6.338AlaAsp: 6.338 ± 0.59
5.773AlaGlu: 5.773 ± 0.704
3.2AlaPhe: 3.2 ± 0.535
7.718AlaGly: 7.718 ± 0.673
1.255AlaHis: 1.255 ± 0.308
4.895AlaIle: 4.895 ± 0.616
3.953AlaLys: 3.953 ± 0.438
8.848AlaLeu: 8.848 ± 0.856
2.385AlaMet: 2.385 ± 0.42
2.887AlaAsn: 2.887 ± 0.459
4.895AlaPro: 4.895 ± 0.698
3.012AlaGln: 3.012 ± 0.506
6.401AlaArg: 6.401 ± 0.549
5.146AlaSer: 5.146 ± 0.618
5.899AlaThr: 5.899 ± 0.613
8.409AlaVal: 8.409 ± 0.807
1.694AlaTrp: 1.694 ± 0.327
2.887AlaTyr: 2.887 ± 0.421
0.0AlaXaa: 0.0 ± 0.0
Cys
0.565CysAla: 0.565 ± 0.184
0.0CysCys: 0.0 ± 0.0
0.377CysAsp: 0.377 ± 0.145
0.753CysGlu: 0.753 ± 0.21
0.126CysPhe: 0.126 ± 0.095
0.565CysGly: 0.565 ± 0.203
0.251CysHis: 0.251 ± 0.128
0.251CysIle: 0.251 ± 0.126
0.502CysLys: 0.502 ± 0.204
0.439CysLeu: 0.439 ± 0.162
0.188CysMet: 0.188 ± 0.13
0.314CysAsn: 0.314 ± 0.141
0.314CysPro: 0.314 ± 0.149
0.314CysGln: 0.314 ± 0.121
0.439CysArg: 0.439 ± 0.172
0.377CysSer: 0.377 ± 0.144
0.314CysThr: 0.314 ± 0.14
0.251CysVal: 0.251 ± 0.129
0.188CysTrp: 0.188 ± 0.098
0.188CysTyr: 0.188 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
6.589AspAla: 6.589 ± 0.746
0.565AspCys: 0.565 ± 0.17
4.706AspAsp: 4.706 ± 0.54
3.953AspGlu: 3.953 ± 0.531
2.322AspPhe: 2.322 ± 0.345
5.773AspGly: 5.773 ± 0.625
1.192AspHis: 1.192 ± 0.311
2.824AspIle: 2.824 ± 0.489
2.51AspLys: 2.51 ± 0.394
6.714AspLeu: 6.714 ± 0.699
1.255AspMet: 1.255 ± 0.226
1.632AspAsn: 1.632 ± 0.315
4.518AspPro: 4.518 ± 0.579
1.945AspGln: 1.945 ± 0.394
3.451AspArg: 3.451 ± 0.359
3.2AspSer: 3.2 ± 0.439
3.891AspThr: 3.891 ± 0.428
4.079AspVal: 4.079 ± 0.477
1.757AspTrp: 1.757 ± 0.406
2.071AspTyr: 2.071 ± 0.358
0.0AspXaa: 0.0 ± 0.0
Glu
5.773GluAla: 5.773 ± 0.739
0.377GluCys: 0.377 ± 0.18
4.706GluAsp: 4.706 ± 0.546
5.02GluGlu: 5.02 ± 0.68
2.322GluPhe: 2.322 ± 0.432
3.702GluGly: 3.702 ± 0.563
1.381GluHis: 1.381 ± 0.351
3.514GluIle: 3.514 ± 0.497
2.761GluLys: 2.761 ± 0.491
7.216GluLeu: 7.216 ± 0.6
1.255GluMet: 1.255 ± 0.26
1.569GluAsn: 1.569 ± 0.408
2.636GluPro: 2.636 ± 0.524
2.322GluGln: 2.322 ± 0.408
4.016GluArg: 4.016 ± 0.568
3.64GluSer: 3.64 ± 0.411
4.267GluThr: 4.267 ± 0.56
5.836GluVal: 5.836 ± 0.65
1.757GluTrp: 1.757 ± 0.365
2.636GluTyr: 2.636 ± 0.533
0.0GluXaa: 0.0 ± 0.0
Phe
2.259PheAla: 2.259 ± 0.28
0.314PheCys: 0.314 ± 0.156
3.2PheAsp: 3.2 ± 0.358
1.883PheGlu: 1.883 ± 0.32
0.628PhePhe: 0.628 ± 0.177
3.514PheGly: 3.514 ± 0.438
0.941PheHis: 0.941 ± 0.308
1.506PheIle: 1.506 ± 0.298
1.192PheLys: 1.192 ± 0.285
2.259PheLeu: 2.259 ± 0.458
0.69PheMet: 0.69 ± 0.168
0.941PheAsn: 0.941 ± 0.196
1.569PhePro: 1.569 ± 0.317
0.941PheGln: 0.941 ± 0.209
1.945PheArg: 1.945 ± 0.383
2.259PheSer: 2.259 ± 0.509
1.945PheThr: 1.945 ± 0.305
2.259PheVal: 2.259 ± 0.423
0.502PheTrp: 0.502 ± 0.159
1.067PheTyr: 1.067 ± 0.276
0.0PheXaa: 0.0 ± 0.0
Gly
7.154GlyAla: 7.154 ± 0.877
0.439GlyCys: 0.439 ± 0.157
5.773GlyAsp: 5.773 ± 0.626
4.644GlyGlu: 4.644 ± 0.576
2.949GlyPhe: 2.949 ± 0.545
7.279GlyGly: 7.279 ± 0.982
1.569GlyHis: 1.569 ± 0.333
4.33GlyIle: 4.33 ± 0.624
3.64GlyLys: 3.64 ± 0.573
7.781GlyLeu: 7.781 ± 0.782
1.757GlyMet: 1.757 ± 0.3
3.326GlyAsn: 3.326 ± 0.391
3.891GlyPro: 3.891 ± 0.552
2.322GlyGln: 2.322 ± 0.323
4.706GlyArg: 4.706 ± 0.602
5.459GlySer: 5.459 ± 0.73
4.895GlyThr: 4.895 ± 0.578
5.522GlyVal: 5.522 ± 0.584
2.385GlyTrp: 2.385 ± 0.405
2.636GlyTyr: 2.636 ± 0.32
0.0GlyXaa: 0.0 ± 0.0
His
1.694HisAla: 1.694 ± 0.335
0.0HisCys: 0.0 ± 0.0
1.318HisAsp: 1.318 ± 0.271
1.506HisGlu: 1.506 ± 0.29
0.69HisPhe: 0.69 ± 0.186
1.255HisGly: 1.255 ± 0.334
0.753HisHis: 0.753 ± 0.189
0.816HisIle: 0.816 ± 0.236
1.192HisLys: 1.192 ± 0.308
1.381HisLeu: 1.381 ± 0.337
0.063HisMet: 0.063 ± 0.049
0.251HisAsn: 0.251 ± 0.111
1.192HisPro: 1.192 ± 0.266
0.879HisGln: 0.879 ± 0.237
1.443HisArg: 1.443 ± 0.29
0.628HisSer: 0.628 ± 0.182
1.255HisThr: 1.255 ± 0.255
1.506HisVal: 1.506 ± 0.324
0.565HisTrp: 0.565 ± 0.151
0.753HisTyr: 0.753 ± 0.246
0.0HisXaa: 0.0 ± 0.0
Ile
6.15IleAla: 6.15 ± 0.752
0.251IleCys: 0.251 ± 0.122
3.389IleAsp: 3.389 ± 0.358
3.891IleGlu: 3.891 ± 0.392
1.004IlePhe: 1.004 ± 0.282
3.828IleGly: 3.828 ± 0.448
0.753IleHis: 0.753 ± 0.2
1.757IleIle: 1.757 ± 0.347
1.443IleLys: 1.443 ± 0.315
3.577IleLeu: 3.577 ± 0.495
0.816IleMet: 0.816 ± 0.251
2.134IleAsn: 2.134 ± 0.338
3.138IlePro: 3.138 ± 0.489
1.569IleGln: 1.569 ± 0.353
3.451IleArg: 3.451 ± 0.411
3.451IleSer: 3.451 ± 0.474
3.514IleThr: 3.514 ± 0.459
2.949IleVal: 2.949 ± 0.525
0.69IleTrp: 0.69 ± 0.179
1.381IleTyr: 1.381 ± 0.263
0.0IleXaa: 0.0 ± 0.0
Lys
3.64LysAla: 3.64 ± 0.505
0.188LysCys: 0.188 ± 0.111
2.259LysAsp: 2.259 ± 0.438
2.385LysGlu: 2.385 ± 0.373
1.632LysPhe: 1.632 ± 0.285
2.573LysGly: 2.573 ± 0.342
1.004LysHis: 1.004 ± 0.262
2.573LysIle: 2.573 ± 0.442
1.883LysLys: 1.883 ± 0.396
3.2LysLeu: 3.2 ± 0.455
1.13LysMet: 1.13 ± 0.233
1.506LysAsn: 1.506 ± 0.272
2.761LysPro: 2.761 ± 0.451
1.443LysGln: 1.443 ± 0.323
3.389LysArg: 3.389 ± 0.489
2.385LysSer: 2.385 ± 0.403
2.761LysThr: 2.761 ± 0.469
3.012LysVal: 3.012 ± 0.45
0.941LysTrp: 0.941 ± 0.21
1.067LysTyr: 1.067 ± 0.322
0.0LysXaa: 0.0 ± 0.0
Leu
9.475LeuAla: 9.475 ± 0.835
0.439LeuCys: 0.439 ± 0.162
5.961LeuAsp: 5.961 ± 0.533
5.836LeuGlu: 5.836 ± 0.603
2.071LeuPhe: 2.071 ± 0.413
7.279LeuGly: 7.279 ± 0.849
1.506LeuHis: 1.506 ± 0.337
4.832LeuIle: 4.832 ± 0.516
4.204LeuLys: 4.204 ± 0.469
6.087LeuLeu: 6.087 ± 0.6
1.883LeuMet: 1.883 ± 0.34
2.887LeuAsn: 2.887 ± 0.377
5.522LeuPro: 5.522 ± 0.634
2.447LeuGln: 2.447 ± 0.374
6.15LeuArg: 6.15 ± 0.644
5.899LeuSer: 5.899 ± 0.525
6.338LeuThr: 6.338 ± 0.535
4.518LeuVal: 4.518 ± 0.599
1.192LeuTrp: 1.192 ± 0.323
2.698LeuTyr: 2.698 ± 0.432
0.0LeuXaa: 0.0 ± 0.0
Met
2.51MetAla: 2.51 ± 0.297
0.0MetCys: 0.0 ± 0.0
1.13MetAsp: 1.13 ± 0.251
1.506MetGlu: 1.506 ± 0.252
0.565MetPhe: 0.565 ± 0.2
1.506MetGly: 1.506 ± 0.277
0.314MetHis: 0.314 ± 0.126
0.565MetIle: 0.565 ± 0.205
0.879MetLys: 0.879 ± 0.22
1.255MetLeu: 1.255 ± 0.286
0.251MetMet: 0.251 ± 0.117
0.816MetAsn: 0.816 ± 0.212
1.381MetPro: 1.381 ± 0.265
0.69MetGln: 0.69 ± 0.199
1.255MetArg: 1.255 ± 0.276
2.259MetSer: 2.259 ± 0.392
2.447MetThr: 2.447 ± 0.368
1.067MetVal: 1.067 ± 0.261
0.314MetTrp: 0.314 ± 0.129
0.377MetTyr: 0.377 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
3.012AsnAla: 3.012 ± 0.515
0.126AsnCys: 0.126 ± 0.089
2.071AsnAsp: 2.071 ± 0.397
2.008AsnGlu: 2.008 ± 0.359
1.004AsnPhe: 1.004 ± 0.264
3.389AsnGly: 3.389 ± 0.473
0.628AsnHis: 0.628 ± 0.181
1.632AsnIle: 1.632 ± 0.353
0.628AsnLys: 0.628 ± 0.203
2.322AsnLeu: 2.322 ± 0.372
0.628AsnMet: 0.628 ± 0.176
0.816AsnAsn: 0.816 ± 0.2
2.447AsnPro: 2.447 ± 0.377
1.13AsnGln: 1.13 ± 0.275
1.694AsnArg: 1.694 ± 0.341
2.008AsnSer: 2.008 ± 0.388
1.82AsnThr: 1.82 ± 0.302
2.573AsnVal: 2.573 ± 0.445
0.816AsnTrp: 0.816 ± 0.191
1.192AsnTyr: 1.192 ± 0.267
0.0AsnXaa: 0.0 ± 0.0
Pro
5.71ProAla: 5.71 ± 0.593
0.502ProCys: 0.502 ± 0.189
4.142ProAsp: 4.142 ± 0.431
4.079ProGlu: 4.079 ± 0.552
2.071ProPhe: 2.071 ± 0.369
4.895ProGly: 4.895 ± 0.61
0.816ProHis: 0.816 ± 0.224
2.322ProIle: 2.322 ± 0.416
1.945ProLys: 1.945 ± 0.295
4.832ProLeu: 4.832 ± 0.632
0.941ProMet: 0.941 ± 0.213
1.82ProAsn: 1.82 ± 0.328
3.012ProPro: 3.012 ± 0.512
1.632ProGln: 1.632 ± 0.294
2.824ProArg: 2.824 ± 0.544
3.953ProSer: 3.953 ± 0.545
3.953ProThr: 3.953 ± 0.515
3.389ProVal: 3.389 ± 0.471
0.879ProTrp: 0.879 ± 0.288
1.569ProTyr: 1.569 ± 0.339
0.0ProXaa: 0.0 ± 0.0
Gln
3.326GlnAla: 3.326 ± 0.515
0.063GlnCys: 0.063 ± 0.062
1.255GlnAsp: 1.255 ± 0.313
1.82GlnGlu: 1.82 ± 0.292
1.004GlnPhe: 1.004 ± 0.247
2.51GlnGly: 2.51 ± 0.378
0.69GlnHis: 0.69 ± 0.184
3.012GlnIle: 3.012 ± 0.589
1.192GlnLys: 1.192 ± 0.245
3.953GlnLeu: 3.953 ± 0.485
1.067GlnMet: 1.067 ± 0.26
0.377GlnAsn: 0.377 ± 0.147
1.945GlnPro: 1.945 ± 0.397
1.82GlnGln: 1.82 ± 0.377
1.82GlnArg: 1.82 ± 0.384
1.82GlnSer: 1.82 ± 0.297
1.632GlnThr: 1.632 ± 0.302
2.259GlnVal: 2.259 ± 0.34
0.502GlnTrp: 0.502 ± 0.155
0.502GlnTyr: 0.502 ± 0.152
0.0GlnXaa: 0.0 ± 0.0
Arg
6.15ArgAla: 6.15 ± 0.655
0.816ArgCys: 0.816 ± 0.278
2.949ArgAsp: 2.949 ± 0.342
4.644ArgGlu: 4.644 ± 0.64
1.945ArgPhe: 1.945 ± 0.377
4.895ArgGly: 4.895 ± 0.602
1.255ArgHis: 1.255 ± 0.334
3.2ArgIle: 3.2 ± 0.462
3.514ArgLys: 3.514 ± 0.525
6.024ArgLeu: 6.024 ± 0.785
2.008ArgMet: 2.008 ± 0.394
2.322ArgAsn: 2.322 ± 0.485
2.761ArgPro: 2.761 ± 0.459
1.883ArgGln: 1.883 ± 0.27
5.271ArgArg: 5.271 ± 0.703
3.64ArgSer: 3.64 ± 0.492
3.075ArgThr: 3.075 ± 0.475
5.146ArgVal: 5.146 ± 0.621
1.13ArgTrp: 1.13 ± 0.245
1.506ArgTyr: 1.506 ± 0.276
0.0ArgXaa: 0.0 ± 0.0
Ser
6.024SerAla: 6.024 ± 0.823
0.565SerCys: 0.565 ± 0.235
3.326SerAsp: 3.326 ± 0.423
3.64SerGlu: 3.64 ± 0.44
2.134SerPhe: 2.134 ± 0.341
6.275SerGly: 6.275 ± 0.674
1.443SerHis: 1.443 ± 0.308
2.698SerIle: 2.698 ± 0.403
2.51SerLys: 2.51 ± 0.437
5.71SerLeu: 5.71 ± 0.62
1.569SerMet: 1.569 ± 0.37
2.259SerAsn: 2.259 ± 0.392
3.451SerPro: 3.451 ± 0.523
1.82SerGln: 1.82 ± 0.317
3.263SerArg: 3.263 ± 0.377
3.263SerSer: 3.263 ± 0.637
3.326SerThr: 3.326 ± 0.528
3.577SerVal: 3.577 ± 0.363
1.443SerTrp: 1.443 ± 0.337
1.945SerTyr: 1.945 ± 0.392
0.0SerXaa: 0.0 ± 0.0
Thr
6.15ThrAla: 6.15 ± 0.794
0.439ThrCys: 0.439 ± 0.23
3.765ThrAsp: 3.765 ± 0.43
4.769ThrGlu: 4.769 ± 0.529
2.196ThrPhe: 2.196 ± 0.368
6.526ThrGly: 6.526 ± 0.686
0.941ThrHis: 0.941 ± 0.258
2.573ThrIle: 2.573 ± 0.641
2.698ThrLys: 2.698 ± 0.342
6.212ThrLeu: 6.212 ± 0.597
0.879ThrMet: 0.879 ± 0.217
1.945ThrAsn: 1.945 ± 0.297
3.765ThrPro: 3.765 ± 0.535
1.883ThrGln: 1.883 ± 0.364
3.702ThrArg: 3.702 ± 0.584
3.577ThrSer: 3.577 ± 0.476
4.581ThrThr: 4.581 ± 0.517
5.522ThrVal: 5.522 ± 0.643
1.192ThrTrp: 1.192 ± 0.246
2.008ThrTyr: 2.008 ± 0.317
0.0ThrXaa: 0.0 ± 0.0
Val
6.338ValAla: 6.338 ± 0.909
0.565ValCys: 0.565 ± 0.162
5.71ValAsp: 5.71 ± 0.653
5.02ValGlu: 5.02 ± 0.537
2.385ValPhe: 2.385 ± 0.364
4.518ValGly: 4.518 ± 0.736
1.443ValHis: 1.443 ± 0.252
3.2ValIle: 3.2 ± 0.351
3.514ValLys: 3.514 ± 0.537
4.769ValLeu: 4.769 ± 0.605
1.255ValMet: 1.255 ± 0.312
2.259ValAsn: 2.259 ± 0.387
3.953ValPro: 3.953 ± 0.402
2.447ValGln: 2.447 ± 0.375
4.832ValArg: 4.832 ± 0.636
4.581ValSer: 4.581 ± 0.574
5.459ValThr: 5.459 ± 0.591
4.644ValVal: 4.644 ± 0.624
1.13ValTrp: 1.13 ± 0.246
2.51ValTyr: 2.51 ± 0.464
0.0ValXaa: 0.0 ± 0.0
Trp
1.381TrpAla: 1.381 ± 0.254
0.314TrpCys: 0.314 ± 0.138
1.318TrpAsp: 1.318 ± 0.289
1.067TrpGlu: 1.067 ± 0.245
0.879TrpPhe: 0.879 ± 0.255
1.82TrpGly: 1.82 ± 0.362
0.377TrpHis: 0.377 ± 0.153
1.192TrpIle: 1.192 ± 0.226
0.314TrpLys: 0.314 ± 0.164
2.008TrpLeu: 2.008 ± 0.377
0.377TrpMet: 0.377 ± 0.179
0.69TrpAsn: 0.69 ± 0.224
0.69TrpPro: 0.69 ± 0.217
0.879TrpGln: 0.879 ± 0.222
1.192TrpArg: 1.192 ± 0.304
1.067TrpSer: 1.067 ± 0.226
1.694TrpThr: 1.694 ± 0.398
2.196TrpVal: 2.196 ± 0.303
0.565TrpTrp: 0.565 ± 0.201
0.188TrpTyr: 0.188 ± 0.101
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.259TyrAla: 2.259 ± 0.444
0.188TyrCys: 0.188 ± 0.105
1.255TyrAsp: 1.255 ± 0.327
2.51TyrGlu: 2.51 ± 0.341
0.69TyrPhe: 0.69 ± 0.199
2.636TyrGly: 2.636 ± 0.419
0.628TyrHis: 0.628 ± 0.203
1.443TyrIle: 1.443 ± 0.346
1.255TyrLys: 1.255 ± 0.214
2.636TyrLeu: 2.636 ± 0.388
0.628TyrMet: 0.628 ± 0.179
1.192TyrAsn: 1.192 ± 0.277
1.443TyrPro: 1.443 ± 0.268
1.192TyrGln: 1.192 ± 0.256
2.887TyrArg: 2.887 ± 0.369
1.694TyrSer: 1.694 ± 0.33
2.259TyrThr: 2.259 ± 0.355
1.82TyrVal: 1.82 ± 0.336
0.565TyrTrp: 0.565 ± 0.184
0.628TyrTyr: 0.628 ± 0.194
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (15937 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski