Pedosphaera parvula (strain Ellin514)

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Verrucomicrobia subdivision 3; Pedosphaera; Pedosphaera parvula

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6492 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9XT45|B9XT45_PEDPL FGE-sulfatase domain-containing protein (Fragment) OS=Pedosphaera parvula (strain Ellin514) OX=320771 GN=Cflav_PD0028 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84RR3 pKa = 11.84NIHH6 pKa = 5.17TLIGLGLIWAGSAPAASVLFDD27 pKa = 4.24FNSDD31 pKa = 3.22PTAGGLATIYY41 pKa = 10.33GASTSWVPSDD51 pKa = 3.24GAGYY55 pKa = 8.0STNASDD61 pKa = 5.53GYY63 pKa = 10.93LQITPAHH70 pKa = 6.25NSQTGAIVFSDD81 pKa = 3.94FDD83 pKa = 3.47NGQIVGGFHH92 pKa = 6.31MEE94 pKa = 3.63ADD96 pKa = 3.65VRR98 pKa = 11.84IGNGTGGTTPADD110 pKa = 3.74GFCIAFARR118 pKa = 11.84EE119 pKa = 3.87NDD121 pKa = 3.66PALANPADD129 pKa = 3.85STKK132 pKa = 10.82YY133 pKa = 10.96ALDD136 pKa = 3.61SGNAQGPEE144 pKa = 3.94EE145 pKa = 4.31GTTTGIAVGFDD156 pKa = 3.37AYY158 pKa = 10.88ANGGTDD164 pKa = 2.94IRR166 pKa = 11.84GIDD169 pKa = 3.74LRR171 pKa = 11.84VDD173 pKa = 3.49GALTVFPMPTLNGSVTDD190 pKa = 3.68TTSIQTGPNDD200 pKa = 3.82GTGSPDD206 pKa = 3.33TLGWAHH212 pKa = 6.77LVVDD216 pKa = 5.25LSTSGTLNVYY226 pKa = 9.04YY227 pKa = 10.86

Molecular weight: 23.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9XDR1|B9XDR1_PEDPL Uncharacterized protein OS=Pedosphaera parvula (strain Ellin514) OX=320771 GN=Cflav_PD6483 PE=4 SV=1
MM1 pKa = 7.68ARR3 pKa = 11.84RR4 pKa = 11.84NRR6 pKa = 11.84QRR8 pKa = 11.84LPVGVRR14 pKa = 11.84LIMFLLQNSKK24 pKa = 10.27QLLALPTTATVPP36 pKa = 3.39

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6492

0

6492

2197852

30

5666

338.5

37.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.405 ± 0.031

1.036 ± 0.01

4.743 ± 0.023

5.4 ± 0.042

4.219 ± 0.02

8.002 ± 0.042

2.089 ± 0.016

5.402 ± 0.023

4.757 ± 0.031

9.885 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.014

4.349 ± 0.035

5.079 ± 0.022

3.9 ± 0.021

5.425 ± 0.034

6.542 ± 0.034

6.124 ± 0.044

7.024 ± 0.025

1.625 ± 0.016

2.805 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski