Sierra dome spider associated circular virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus siedo1

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BP85|A0A346BP85_9VIRU Putative capsid protein OS=Sierra dome spider associated circular virus 1 OX=2293299 PE=4 SV=1
MM1 pKa = 7.4LFVNSKK7 pKa = 10.5YY8 pKa = 11.07VLLTFAQCGDD18 pKa = 3.5LDD20 pKa = 3.67EE21 pKa = 4.61WHH23 pKa = 6.87VSDD26 pKa = 5.14HH27 pKa = 6.37LSSLGAEE34 pKa = 4.27CIVARR39 pKa = 11.84EE40 pKa = 3.99VHH42 pKa = 6.0PTTGGVHH49 pKa = 5.86LHH51 pKa = 5.79VFVDD55 pKa = 4.54FGRR58 pKa = 11.84KK59 pKa = 7.99FRR61 pKa = 11.84SRR63 pKa = 11.84NVRR66 pKa = 11.84VFDD69 pKa = 3.45VDD71 pKa = 3.28GRR73 pKa = 11.84HH74 pKa = 6.11PNVVPSRR81 pKa = 11.84GTPEE85 pKa = 3.88KK86 pKa = 10.92GFDD89 pKa = 3.46YY90 pKa = 10.65AIKK93 pKa = 10.86DD94 pKa = 3.51GDD96 pKa = 4.01VVAGGLEE103 pKa = 3.89RR104 pKa = 11.84PMPRR108 pKa = 11.84GGMSVGAEE116 pKa = 4.17NIRR119 pKa = 11.84NVAGLCEE126 pKa = 4.32STTEE130 pKa = 3.87FLEE133 pKa = 5.37LFDD136 pKa = 4.33EE137 pKa = 4.48MDD139 pKa = 3.58RR140 pKa = 11.84GTLFKK145 pKa = 10.89SFTNIRR151 pKa = 11.84SYY153 pKa = 11.64ADD155 pKa = 2.71WRR157 pKa = 11.84FAVEE161 pKa = 4.11PEE163 pKa = 4.38PYY165 pKa = 9.98VPPFGTEE172 pKa = 3.72FSGGEE177 pKa = 3.68FDD179 pKa = 6.42GRR181 pKa = 11.84DD182 pKa = 3.16DD183 pKa = 3.53WLAQSGIGSKK193 pKa = 10.31SLILYY198 pKa = 8.68GPSRR202 pKa = 11.84TGKK205 pKa = 8.43TSWARR210 pKa = 11.84SLGAHH215 pKa = 7.18LYY217 pKa = 10.21FEE219 pKa = 4.91RR220 pKa = 11.84LFSGKK225 pKa = 8.65EE226 pKa = 4.07AINHH230 pKa = 4.95MSEE233 pKa = 3.66VEE235 pKa = 4.06YY236 pKa = 10.91AVFDD240 pKa = 4.18DD241 pKa = 4.85CSINHH246 pKa = 5.1MPGWKK251 pKa = 9.23SWFGAQATVGVRR263 pKa = 11.84ALYY266 pKa = 10.45RR267 pKa = 11.84DD268 pKa = 3.52ATYY271 pKa = 10.87VAWGKK276 pKa = 9.77PIVWCTNRR284 pKa = 11.84DD285 pKa = 3.12PRR287 pKa = 11.84IDD289 pKa = 3.51MRR291 pKa = 11.84IDD293 pKa = 2.99IDD295 pKa = 3.82NEE297 pKa = 3.89RR298 pKa = 11.84SHH300 pKa = 6.57MWFQDD305 pKa = 3.82DD306 pKa = 4.3IDD308 pKa = 3.78WMEE311 pKa = 4.26ANCVFINITDD321 pKa = 3.57NLYY324 pKa = 10.98

Molecular weight: 36.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BP85|A0A346BP85_9VIRU Putative capsid protein OS=Sierra dome spider associated circular virus 1 OX=2293299 PE=4 SV=1
MM1 pKa = 7.36RR2 pKa = 11.84TRR4 pKa = 11.84ARR6 pKa = 11.84RR7 pKa = 11.84TRR9 pKa = 11.84RR10 pKa = 11.84SFARR14 pKa = 11.84SKK16 pKa = 10.46RR17 pKa = 11.84PRR19 pKa = 11.84RR20 pKa = 11.84SYY22 pKa = 10.44LPSRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84SGGKK32 pKa = 9.24KK33 pKa = 7.49RR34 pKa = 11.84TYY36 pKa = 9.8RR37 pKa = 11.84KK38 pKa = 9.55KK39 pKa = 10.64GSRR42 pKa = 11.84KK43 pKa = 8.87MILNMTSTKK52 pKa = 10.4KK53 pKa = 10.01RR54 pKa = 11.84DD55 pKa = 3.25TMINYY60 pKa = 7.93TNVTGTSQIGGTAYY74 pKa = 9.13STSPAIITGGYY85 pKa = 4.77TTFNPFVWVATARR98 pKa = 11.84DD99 pKa = 3.67NTTANSGGAGLKK111 pKa = 9.81FFQSTRR117 pKa = 11.84TATTCFMRR125 pKa = 11.84GLAEE129 pKa = 4.35SIEE132 pKa = 4.19IQVSDD137 pKa = 3.79GLPWQWRR144 pKa = 11.84RR145 pKa = 11.84ICFTYY150 pKa = 10.31KK151 pKa = 10.72GIADD155 pKa = 5.96LIPSVTTAPAFSLSQEE171 pKa = 4.33TSSGWVRR178 pKa = 11.84TVNALPNNTYY188 pKa = 10.28RR189 pKa = 11.84DD190 pKa = 3.51NFEE193 pKa = 4.11AVIFRR198 pKa = 11.84GSKK201 pKa = 10.36GVDD204 pKa = 3.72FSEE207 pKa = 4.89PLTAPLDD214 pKa = 3.7PTRR217 pKa = 11.84ITVKK221 pKa = 10.16YY222 pKa = 10.36DD223 pKa = 3.0KK224 pKa = 10.49TRR226 pKa = 11.84TIASGNEE233 pKa = 3.71DD234 pKa = 3.36GCIRR238 pKa = 11.84KK239 pKa = 9.63YY240 pKa = 10.98KK241 pKa = 9.76FWHH244 pKa = 5.91GMNKK248 pKa = 9.61NLVYY252 pKa = 10.79ADD254 pKa = 4.34DD255 pKa = 4.01EE256 pKa = 5.04SGGVEE261 pKa = 3.72NVGYY265 pKa = 10.59FSTQSKK271 pKa = 10.98AGMGDD276 pKa = 3.82YY277 pKa = 10.98VVVDD281 pKa = 3.39YY282 pKa = 8.86FLPRR286 pKa = 11.84TGSTSANKK294 pKa = 10.38LSFNATASLYY304 pKa = 8.39WHH306 pKa = 7.02EE307 pKa = 4.37KK308 pKa = 9.19

Molecular weight: 34.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

632

308

324

316.0

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.646 ± 0.35

1.424 ± 0.316

6.171 ± 1.371

4.589 ± 1.171

5.696 ± 0.581

8.861 ± 0.295

1.899 ± 0.878

4.589 ± 0.03

4.589 ± 1.111

5.38 ± 0.586

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.182

4.589 ± 0.426

4.114 ± 0.153

1.582 ± 0.257

8.228 ± 0.835

8.07 ± 0.946

8.07 ± 2.543

6.646 ± 1.02

2.532 ± 0.41

3.797 ± 0.526
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski