Japanese iris necrotic ring virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Betacarmovirus

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9IRA0|Q9IRA0_9TOMB Replicase (P26) OS=Japanese iris necrotic ring virus OX=77344 PE=4 SV=1
MM1 pKa = 8.08DD2 pKa = 4.67SASEE6 pKa = 4.19TTTYY10 pKa = 10.99EE11 pKa = 3.78LDD13 pKa = 3.7PPGEE17 pKa = 4.07EE18 pKa = 4.12LPTVKK23 pKa = 10.4RR24 pKa = 11.84EE25 pKa = 3.8IQNARR30 pKa = 11.84GSQATKK36 pKa = 9.99RR37 pKa = 11.84AVARR41 pKa = 11.84DD42 pKa = 3.43AALSTVRR49 pKa = 11.84SVASRR54 pKa = 11.84EE55 pKa = 4.04VVGGVWVQVGEE66 pKa = 4.42TFTTTNHH73 pKa = 5.2FHH75 pKa = 6.81FF76 pKa = 5.33

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9IR99|Q9IR99_9TOMB Movement protein (P8) OS=Japanese iris necrotic ring virus OX=77344 PE=4 SV=1
MM1 pKa = 7.48PGAVKK6 pKa = 10.46RR7 pKa = 11.84LRR9 pKa = 11.84GLLRR13 pKa = 11.84GMLRR17 pKa = 11.84CLQFALWLVGKK28 pKa = 9.8LLVGFGCRR36 pKa = 11.84SARR39 pKa = 11.84PSPQPTIFTSEE50 pKa = 4.27PLWDD54 pKa = 3.88NDD56 pKa = 3.74LLLVLIISLTFTLIYY71 pKa = 10.26IFAQQQPVHH80 pKa = 5.76HH81 pKa = 6.3HH82 pKa = 6.74HH83 pKa = 6.38YY84 pKa = 10.61SEE86 pKa = 4.97SNHH89 pKa = 5.31KK90 pKa = 7.43TQHH93 pKa = 4.67ITIGTEE99 pKa = 3.64SQTRR103 pKa = 11.84QIPNGATT110 pKa = 3.04

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2432

76

888

405.3

45.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.319 ± 0.521

2.467 ± 0.359

3.783 ± 0.22

5.263 ± 0.43

4.359 ± 0.146

6.949 ± 0.287

2.878 ± 0.356

4.77 ± 0.341

3.947 ± 0.368

9.252 ± 0.484

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.166

4.112 ± 0.353

5.222 ± 0.242

4.153 ± 0.21

6.949 ± 0.203

7.484 ± 0.645

6.373 ± 0.898

8.1 ± 0.429

1.439 ± 0.117

2.961 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski