Amino acid dipepetide frequency for Ralstonia phage Dimitile

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.711AlaAla: 20.711 ± 1.148
1.129AlaCys: 1.129 ± 0.366
6.49AlaAsp: 6.49 ± 0.588
7.957AlaGlu: 7.957 ± 1.011
3.612AlaPhe: 3.612 ± 0.457
11.682AlaGly: 11.682 ± 1.138
1.975AlaHis: 1.975 ± 0.317
5.079AlaIle: 5.079 ± 0.658
6.208AlaLys: 6.208 ± 0.771
10.158AlaLeu: 10.158 ± 0.58
3.668AlaMet: 3.668 ± 0.447
4.289AlaAsn: 4.289 ± 0.607
7.506AlaPro: 7.506 ± 0.863
6.546AlaGln: 6.546 ± 0.518
7.393AlaArg: 7.393 ± 0.775
7.393AlaSer: 7.393 ± 0.64
5.643AlaThr: 5.643 ± 0.497
7.167AlaVal: 7.167 ± 0.547
1.58AlaTrp: 1.58 ± 0.339
3.16AlaTyr: 3.16 ± 0.372
0.0AlaXaa: 0.0 ± 0.0
Cys
0.339CysAla: 0.339 ± 0.158
0.339CysCys: 0.339 ± 0.142
0.564CysAsp: 0.564 ± 0.271
0.451CysGlu: 0.451 ± 0.23
0.226CysPhe: 0.226 ± 0.173
0.734CysGly: 0.734 ± 0.267
0.226CysHis: 0.226 ± 0.124
0.339CysIle: 0.339 ± 0.144
0.451CysLys: 0.451 ± 0.184
0.508CysLeu: 0.508 ± 0.254
0.282CysMet: 0.282 ± 0.16
0.339CysAsn: 0.339 ± 0.133
0.226CysPro: 0.226 ± 0.13
0.169CysGln: 0.169 ± 0.134
0.621CysArg: 0.621 ± 0.256
0.621CysSer: 0.621 ± 0.235
0.226CysThr: 0.226 ± 0.133
0.564CysVal: 0.564 ± 0.232
0.113CysTrp: 0.113 ± 0.075
0.282CysTyr: 0.282 ± 0.201
0.0CysXaa: 0.0 ± 0.0
Asp
8.183AspAla: 8.183 ± 0.765
0.282AspCys: 0.282 ± 0.152
3.33AspAsp: 3.33 ± 0.691
3.894AspGlu: 3.894 ± 0.485
1.975AspPhe: 1.975 ± 0.283
7.506AspGly: 7.506 ± 0.975
1.185AspHis: 1.185 ± 0.225
2.822AspIle: 2.822 ± 0.305
1.919AspLys: 1.919 ± 0.308
4.063AspLeu: 4.063 ± 0.426
1.637AspMet: 1.637 ± 0.273
2.032AspAsn: 2.032 ± 0.316
2.652AspPro: 2.652 ± 0.376
1.58AspGln: 1.58 ± 0.282
4.289AspArg: 4.289 ± 0.479
2.652AspSer: 2.652 ± 0.468
2.878AspThr: 2.878 ± 0.326
4.233AspVal: 4.233 ± 0.49
0.903AspTrp: 0.903 ± 0.201
2.201AspTyr: 2.201 ± 0.279
0.0AspXaa: 0.0 ± 0.0
Glu
6.772GluAla: 6.772 ± 0.967
0.564GluCys: 0.564 ± 0.201
3.668GluAsp: 3.668 ± 0.415
3.781GluGlu: 3.781 ± 0.498
3.047GluPhe: 3.047 ± 0.516
4.345GluGly: 4.345 ± 0.748
1.58GluHis: 1.58 ± 0.31
3.16GluIle: 3.16 ± 0.366
2.822GluLys: 2.822 ± 0.769
5.53GluLeu: 5.53 ± 0.5
1.411GluMet: 1.411 ± 0.2
2.314GluAsn: 2.314 ± 0.325
2.878GluPro: 2.878 ± 0.647
3.555GluGln: 3.555 ± 0.433
6.264GluArg: 6.264 ± 0.822
2.822GluSer: 2.822 ± 0.386
2.596GluThr: 2.596 ± 0.317
3.16GluVal: 3.16 ± 0.423
0.847GluTrp: 0.847 ± 0.196
1.806GluTyr: 1.806 ± 0.362
0.0GluXaa: 0.0 ± 0.0
Phe
3.386PheAla: 3.386 ± 0.425
0.226PheCys: 0.226 ± 0.121
3.273PheAsp: 3.273 ± 0.384
1.862PheGlu: 1.862 ± 0.35
0.734PhePhe: 0.734 ± 0.237
2.878PheGly: 2.878 ± 0.382
0.564PheHis: 0.564 ± 0.198
1.467PheIle: 1.467 ± 0.407
1.467PheLys: 1.467 ± 0.249
1.862PheLeu: 1.862 ± 0.351
0.903PheMet: 0.903 ± 0.177
1.185PheAsn: 1.185 ± 0.22
1.129PhePro: 1.129 ± 0.203
0.959PheGln: 0.959 ± 0.177
1.862PheArg: 1.862 ± 0.46
2.314PheSer: 2.314 ± 0.319
1.185PheThr: 1.185 ± 0.269
2.709PheVal: 2.709 ± 0.38
0.282PheTrp: 0.282 ± 0.114
1.185PheTyr: 1.185 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
11.795GlyAla: 11.795 ± 1.383
0.451GlyCys: 0.451 ± 0.22
5.926GlyAsp: 5.926 ± 0.624
5.756GlyGlu: 5.756 ± 0.697
2.652GlyPhe: 2.652 ± 0.301
8.916GlyGly: 8.916 ± 1.217
1.467GlyHis: 1.467 ± 0.366
3.442GlyIle: 3.442 ± 0.311
5.079GlyLys: 5.079 ± 0.543
5.135GlyLeu: 5.135 ± 0.556
2.37GlyMet: 2.37 ± 0.434
3.16GlyAsn: 3.16 ± 0.615
1.975GlyPro: 1.975 ± 0.272
3.16GlyGln: 3.16 ± 0.478
6.377GlyArg: 6.377 ± 0.591
4.853GlySer: 4.853 ± 0.656
5.643GlyThr: 5.643 ± 0.714
5.813GlyVal: 5.813 ± 0.627
1.185GlyTrp: 1.185 ± 0.253
2.088GlyTyr: 2.088 ± 0.348
0.0GlyXaa: 0.0 ± 0.0
His
2.37HisAla: 2.37 ± 0.321
0.226HisCys: 0.226 ± 0.12
1.298HisAsp: 1.298 ± 0.243
1.467HisGlu: 1.467 ± 0.234
0.847HisPhe: 0.847 ± 0.223
1.298HisGly: 1.298 ± 0.259
0.282HisHis: 0.282 ± 0.113
0.79HisIle: 0.79 ± 0.219
0.734HisLys: 0.734 ± 0.226
0.903HisLeu: 0.903 ± 0.238
0.621HisMet: 0.621 ± 0.178
0.621HisAsn: 0.621 ± 0.159
0.677HisPro: 0.677 ± 0.179
0.621HisGln: 0.621 ± 0.156
1.524HisArg: 1.524 ± 0.286
0.959HisSer: 0.959 ± 0.243
0.79HisThr: 0.79 ± 0.174
1.129HisVal: 1.129 ± 0.218
0.169HisTrp: 0.169 ± 0.134
0.621HisTyr: 0.621 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
6.208IleAla: 6.208 ± 0.586
0.169IleCys: 0.169 ± 0.127
2.709IleAsp: 2.709 ± 0.37
4.289IleGlu: 4.289 ± 0.472
0.959IlePhe: 0.959 ± 0.233
3.95IleGly: 3.95 ± 0.406
0.621IleHis: 0.621 ± 0.152
1.524IleIle: 1.524 ± 0.319
2.032IleLys: 2.032 ± 0.362
2.314IleLeu: 2.314 ± 0.287
1.072IleMet: 1.072 ± 0.339
1.975IleAsn: 1.975 ± 0.243
1.749IlePro: 1.749 ± 0.293
1.919IleGln: 1.919 ± 0.379
3.273IleArg: 3.273 ± 0.612
2.314IleSer: 2.314 ± 0.353
2.314IleThr: 2.314 ± 0.409
3.217IleVal: 3.217 ± 0.534
0.226IleTrp: 0.226 ± 0.091
1.016IleTyr: 1.016 ± 0.24
0.0IleXaa: 0.0 ± 0.0
Lys
7.167LysAla: 7.167 ± 0.514
0.451LysCys: 0.451 ± 0.199
3.104LysAsp: 3.104 ± 0.442
2.822LysGlu: 2.822 ± 0.683
1.298LysPhe: 1.298 ± 0.248
3.33LysGly: 3.33 ± 0.516
0.564LysHis: 0.564 ± 0.17
1.862LysIle: 1.862 ± 0.386
2.314LysLys: 2.314 ± 0.382
3.95LysLeu: 3.95 ± 0.566
1.185LysMet: 1.185 ± 0.336
1.467LysAsn: 1.467 ± 0.315
2.709LysPro: 2.709 ± 0.443
2.709LysGln: 2.709 ± 0.366
3.612LysArg: 3.612 ± 0.466
2.596LysSer: 2.596 ± 0.369
3.104LysThr: 3.104 ± 0.413
2.991LysVal: 2.991 ± 0.363
0.903LysTrp: 0.903 ± 0.246
0.903LysTyr: 0.903 ± 0.17
0.0LysXaa: 0.0 ± 0.0
Leu
6.998LeuAla: 6.998 ± 0.54
0.282LeuCys: 0.282 ± 0.147
5.474LeuAsp: 5.474 ± 0.67
3.612LeuGlu: 3.612 ± 0.53
1.919LeuPhe: 1.919 ± 0.305
6.151LeuGly: 6.151 ± 0.532
1.354LeuHis: 1.354 ± 0.232
3.555LeuIle: 3.555 ± 0.555
4.458LeuLys: 4.458 ± 0.555
4.12LeuLeu: 4.12 ± 0.528
1.862LeuMet: 1.862 ± 0.338
2.54LeuAsn: 2.54 ± 0.325
4.233LeuPro: 4.233 ± 0.902
2.314LeuGln: 2.314 ± 0.302
5.305LeuArg: 5.305 ± 0.581
4.458LeuSer: 4.458 ± 0.477
4.345LeuThr: 4.345 ± 0.442
4.063LeuVal: 4.063 ± 0.668
0.621LeuTrp: 0.621 ± 0.2
1.637LeuTyr: 1.637 ± 0.25
0.0LeuXaa: 0.0 ± 0.0
Met
2.596MetAla: 2.596 ± 0.475
0.169MetCys: 0.169 ± 0.111
1.354MetAsp: 1.354 ± 0.283
1.016MetGlu: 1.016 ± 0.202
0.79MetPhe: 0.79 ± 0.223
2.257MetGly: 2.257 ± 0.346
0.395MetHis: 0.395 ± 0.174
0.903MetIle: 0.903 ± 0.196
1.862MetLys: 1.862 ± 0.286
1.806MetLeu: 1.806 ± 0.255
0.903MetMet: 0.903 ± 0.178
1.072MetAsn: 1.072 ± 0.272
1.637MetPro: 1.637 ± 0.344
1.524MetGln: 1.524 ± 0.335
2.201MetArg: 2.201 ± 0.423
2.201MetSer: 2.201 ± 0.317
1.975MetThr: 1.975 ± 0.373
1.298MetVal: 1.298 ± 0.287
0.451MetTrp: 0.451 ± 0.177
0.508MetTyr: 0.508 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
4.12AsnAla: 4.12 ± 0.485
0.282AsnCys: 0.282 ± 0.143
2.201AsnAsp: 2.201 ± 0.276
2.257AsnGlu: 2.257 ± 0.337
1.129AsnPhe: 1.129 ± 0.265
4.12AsnGly: 4.12 ± 0.543
0.79AsnHis: 0.79 ± 0.224
1.637AsnIle: 1.637 ± 0.287
1.354AsnLys: 1.354 ± 0.294
2.201AsnLeu: 2.201 ± 0.305
0.734AsnMet: 0.734 ± 0.211
1.354AsnAsn: 1.354 ± 0.259
2.37AsnPro: 2.37 ± 0.383
1.58AsnGln: 1.58 ± 0.305
2.201AsnArg: 2.201 ± 0.25
1.975AsnSer: 1.975 ± 0.311
2.314AsnThr: 2.314 ± 0.466
3.33AsnVal: 3.33 ± 0.359
0.677AsnTrp: 0.677 ± 0.153
0.677AsnTyr: 0.677 ± 0.193
0.0AsnXaa: 0.0 ± 0.0
Pro
8.521ProAla: 8.521 ± 1.256
0.282ProCys: 0.282 ± 0.133
2.878ProAsp: 2.878 ± 0.391
3.104ProGlu: 3.104 ± 0.46
1.298ProPhe: 1.298 ± 0.278
3.612ProGly: 3.612 ± 0.558
0.621ProHis: 0.621 ± 0.2
1.862ProIle: 1.862 ± 0.378
2.54ProLys: 2.54 ± 0.368
2.765ProLeu: 2.765 ± 0.344
0.847ProMet: 0.847 ± 0.322
1.411ProAsn: 1.411 ± 0.331
1.975ProPro: 1.975 ± 0.324
1.806ProGln: 1.806 ± 0.374
2.765ProArg: 2.765 ± 0.348
3.555ProSer: 3.555 ± 0.555
2.878ProThr: 2.878 ± 0.395
3.047ProVal: 3.047 ± 0.384
0.282ProTrp: 0.282 ± 0.11
1.016ProTyr: 1.016 ± 0.308
0.0ProXaa: 0.0 ± 0.0
Gln
6.377GlnAla: 6.377 ± 0.86
0.113GlnCys: 0.113 ± 0.083
1.806GlnAsp: 1.806 ± 0.266
2.201GlnGlu: 2.201 ± 0.35
1.016GlnPhe: 1.016 ± 0.252
3.33GlnGly: 3.33 ± 0.504
0.677GlnHis: 0.677 ± 0.176
2.257GlnIle: 2.257 ± 0.395
1.975GlnLys: 1.975 ± 0.339
3.104GlnLeu: 3.104 ± 0.351
1.467GlnMet: 1.467 ± 0.388
1.354GlnAsn: 1.354 ± 0.308
1.862GlnPro: 1.862 ± 0.296
4.063GlnGln: 4.063 ± 0.803
2.935GlnArg: 2.935 ± 0.54
1.919GlnSer: 1.919 ± 0.283
1.975GlnThr: 1.975 ± 0.45
2.822GlnVal: 2.822 ± 0.344
0.734GlnTrp: 0.734 ± 0.2
1.242GlnTyr: 1.242 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
9.199ArgAla: 9.199 ± 0.71
0.451ArgCys: 0.451 ± 0.225
3.725ArgAsp: 3.725 ± 0.395
4.684ArgGlu: 4.684 ± 0.542
3.499ArgPhe: 3.499 ± 0.521
4.345ArgGly: 4.345 ± 0.431
1.467ArgHis: 1.467 ± 0.3
3.386ArgIle: 3.386 ± 0.444
3.273ArgLys: 3.273 ± 0.456
5.135ArgLeu: 5.135 ± 0.501
2.032ArgMet: 2.032 ± 0.319
3.273ArgAsn: 3.273 ± 0.359
2.37ArgPro: 2.37 ± 0.483
3.442ArgGln: 3.442 ± 0.498
4.684ArgArg: 4.684 ± 0.524
3.555ArgSer: 3.555 ± 0.37
2.822ArgThr: 2.822 ± 0.439
4.966ArgVal: 4.966 ± 0.583
1.185ArgTrp: 1.185 ± 0.248
2.822ArgTyr: 2.822 ± 0.384
0.0ArgXaa: 0.0 ± 0.0
Ser
6.828SerAla: 6.828 ± 0.684
0.677SerCys: 0.677 ± 0.25
3.668SerAsp: 3.668 ± 0.426
3.047SerGlu: 3.047 ± 0.376
1.919SerPhe: 1.919 ± 0.277
5.305SerGly: 5.305 ± 0.685
1.242SerHis: 1.242 ± 0.229
2.314SerIle: 2.314 ± 0.306
2.652SerLys: 2.652 ± 0.316
3.95SerLeu: 3.95 ± 0.386
1.242SerMet: 1.242 ± 0.345
2.37SerAsn: 2.37 ± 0.435
2.709SerPro: 2.709 ± 0.341
2.201SerGln: 2.201 ± 0.319
3.781SerArg: 3.781 ± 0.489
2.765SerSer: 2.765 ± 0.415
3.725SerThr: 3.725 ± 0.403
3.668SerVal: 3.668 ± 0.402
1.016SerTrp: 1.016 ± 0.246
1.467SerTyr: 1.467 ± 0.258
0.0SerXaa: 0.0 ± 0.0
Thr
6.151ThrAla: 6.151 ± 0.522
0.451ThrCys: 0.451 ± 0.21
2.596ThrAsp: 2.596 ± 0.344
3.781ThrGlu: 3.781 ± 0.45
1.298ThrPhe: 1.298 ± 0.325
5.53ThrGly: 5.53 ± 0.693
1.016ThrHis: 1.016 ± 0.195
2.765ThrIle: 2.765 ± 0.387
2.991ThrLys: 2.991 ± 0.325
4.628ThrLeu: 4.628 ± 0.599
1.637ThrMet: 1.637 ± 0.31
1.862ThrAsn: 1.862 ± 0.331
3.104ThrPro: 3.104 ± 0.345
1.411ThrGln: 1.411 ± 0.286
2.991ThrArg: 2.991 ± 0.45
2.765ThrSer: 2.765 ± 0.455
3.273ThrThr: 3.273 ± 0.614
4.289ThrVal: 4.289 ± 0.428
0.621ThrTrp: 0.621 ± 0.153
1.637ThrTyr: 1.637 ± 0.264
0.0ThrXaa: 0.0 ± 0.0
Val
7.506ValAla: 7.506 ± 0.576
0.734ValCys: 0.734 ± 0.249
3.612ValAsp: 3.612 ± 0.35
4.402ValGlu: 4.402 ± 0.506
1.749ValPhe: 1.749 ± 0.329
4.966ValGly: 4.966 ± 0.495
1.129ValHis: 1.129 ± 0.277
2.765ValIle: 2.765 ± 0.502
3.273ValLys: 3.273 ± 0.513
3.16ValLeu: 3.16 ± 0.334
1.919ValMet: 1.919 ± 0.313
2.935ValAsn: 2.935 ± 0.429
3.894ValPro: 3.894 ± 0.495
2.54ValGln: 2.54 ± 0.352
4.91ValArg: 4.91 ± 0.519
4.571ValSer: 4.571 ± 0.441
4.345ValThr: 4.345 ± 0.472
4.007ValVal: 4.007 ± 0.517
0.903ValTrp: 0.903 ± 0.259
1.637ValTyr: 1.637 ± 0.327
0.0ValXaa: 0.0 ± 0.0
Trp
1.129TrpAla: 1.129 ± 0.207
0.113TrpCys: 0.113 ± 0.097
0.621TrpAsp: 0.621 ± 0.234
0.508TrpGlu: 0.508 ± 0.21
0.621TrpPhe: 0.621 ± 0.2
1.016TrpGly: 1.016 ± 0.21
0.395TrpHis: 0.395 ± 0.208
0.734TrpIle: 0.734 ± 0.206
0.451TrpLys: 0.451 ± 0.178
1.298TrpLeu: 1.298 ± 0.263
0.508TrpMet: 0.508 ± 0.166
0.677TrpAsn: 0.677 ± 0.177
0.451TrpPro: 0.451 ± 0.195
0.395TrpGln: 0.395 ± 0.128
1.242TrpArg: 1.242 ± 0.294
0.734TrpSer: 0.734 ± 0.226
0.903TrpThr: 0.903 ± 0.202
0.847TrpVal: 0.847 ± 0.175
0.226TrpTrp: 0.226 ± 0.159
0.282TrpTyr: 0.282 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.991TyrAla: 2.991 ± 0.4
0.226TyrCys: 0.226 ± 0.12
1.919TyrAsp: 1.919 ± 0.196
2.032TyrGlu: 2.032 ± 0.295
0.903TyrPhe: 0.903 ± 0.265
1.975TyrGly: 1.975 ± 0.337
0.395TyrHis: 0.395 ± 0.126
1.185TyrIle: 1.185 ± 0.252
1.185TyrLys: 1.185 ± 0.208
2.709TyrLeu: 2.709 ± 0.469
0.564TyrMet: 0.564 ± 0.166
1.072TyrAsn: 1.072 ± 0.225
0.959TyrPro: 0.959 ± 0.241
0.734TyrGln: 0.734 ± 0.177
2.088TyrArg: 2.088 ± 0.321
1.524TyrSer: 1.524 ± 0.311
1.862TyrThr: 1.862 ± 0.277
1.637TyrVal: 1.637 ± 0.352
0.226TyrTrp: 0.226 ± 0.192
0.621TyrTyr: 0.621 ± 0.141
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (17721 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski