Picea glauca (White spruce) (Pinus glauca)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Acrogymnospermae; Pinopsida; Pinidae; Conifers I; Pinaceae; Picea

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A101LWJ3|A0A101LWJ3_PICGL Uncharacterized protein OS=Picea glauca OX=3330 GN=ABT39_MTgene1569 PE=4 SV=1
MM1 pKa = 7.97DD2 pKa = 5.04EE3 pKa = 4.62KK4 pKa = 11.18DD5 pKa = 4.17PYY7 pKa = 10.14PALLGIDD14 pKa = 3.46WAFDD18 pKa = 2.99NYY20 pKa = 11.31AIIDD24 pKa = 4.0LKK26 pKa = 10.69KK27 pKa = 8.92GTMMLEE33 pKa = 3.99ADD35 pKa = 4.17VTSVLQPLDD44 pKa = 3.95PYY46 pKa = 10.44EE47 pKa = 4.6GPWYY51 pKa = 9.4TEE53 pKa = 4.31PIEE56 pKa = 4.71EE57 pKa = 4.24GLEE60 pKa = 3.85DD61 pKa = 3.42TMLNQIYY68 pKa = 10.26NLTAGRR74 pKa = 11.84QEE76 pKa = 5.39DD77 pKa = 5.0YY78 pKa = 11.06INPTIDD84 pKa = 4.58GPVSWRR90 pKa = 11.84SFHH93 pKa = 7.31SFDD96 pKa = 4.27SDD98 pKa = 4.34SEE100 pKa = 4.06EE101 pKa = 4.2AMYY104 pKa = 11.02NN105 pKa = 3.44

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A101M358|A0A101M358_PICGL Uncharacterized protein OS=Picea glauca OX=3330 GN=ABT39_MTgene3315 PE=4 SV=1
MM1 pKa = 7.69MIRR4 pKa = 11.84IRR6 pKa = 11.84LFTIVTSTLPIVTLRR21 pKa = 11.84NLLVLRR27 pKa = 11.84LLLNLTSRR35 pKa = 11.84RR36 pKa = 11.84TPLTIRR42 pKa = 11.84IHH44 pKa = 5.51LTHH47 pKa = 6.84HH48 pKa = 6.57LLLRR52 pKa = 11.84LQRR55 pKa = 11.84PLTLPISRR63 pKa = 11.84LMRR66 pKa = 11.84FQILMTLL73 pKa = 3.88

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6155

0

6155

491974

29

2126

79.9

9.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.147 ± 0.044

2.314 ± 0.029

3.459 ± 0.034

4.005 ± 0.045

4.424 ± 0.041

6.057 ± 0.044

2.916 ± 0.029

5.841 ± 0.048

4.192 ± 0.042

13.106 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.747 ± 0.039

3.478 ± 0.034

6.472 ± 0.056

3.483 ± 0.036

6.704 ± 0.047

8.983 ± 0.05

5.164 ± 0.036

5.251 ± 0.041

1.627 ± 0.024

3.628 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski