Mycobacterium phage Rando14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385D540|A0A385D540_9CAUD Uncharacterized protein OS=Mycobacterium phage Rando14 OX=2301556 GN=70 PE=4 SV=1
MM1 pKa = 7.22QHH3 pKa = 7.08DD4 pKa = 4.9CDD6 pKa = 5.39GDD8 pKa = 3.9DD9 pKa = 5.48CGVCDD14 pKa = 4.38ARR16 pKa = 11.84ITEE19 pKa = 4.29RR20 pKa = 11.84EE21 pKa = 3.95YY22 pKa = 11.23HH23 pKa = 6.53RR24 pKa = 11.84DD25 pKa = 3.21IPVDD29 pKa = 3.56VPDD32 pKa = 5.7FYY34 pKa = 11.63DD35 pKa = 3.62GTT37 pKa = 4.05

Molecular weight: 4.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385D5C0|A0A385D5C0_9CAUD Uncharacterized protein OS=Mycobacterium phage Rando14 OX=2301556 GN=61 PE=4 SV=1
MM1 pKa = 8.13SSGQRR6 pKa = 11.84NAVCWTTCPHH16 pKa = 6.08CGKK19 pKa = 10.25RR20 pKa = 11.84SYY22 pKa = 11.36ASRR25 pKa = 11.84KK26 pKa = 5.55EE27 pKa = 3.77AKK29 pKa = 9.67RR30 pKa = 11.84AVSRR34 pKa = 11.84FRR36 pKa = 11.84GDD38 pKa = 2.77RR39 pKa = 11.84GGHH42 pKa = 4.46MSPYY46 pKa = 9.68RR47 pKa = 11.84CHH49 pKa = 6.91EE50 pKa = 4.42DD51 pKa = 2.97QTRR54 pKa = 11.84WHH56 pKa = 6.48VGHH59 pKa = 7.42LHH61 pKa = 5.18TATRR65 pKa = 11.84RR66 pKa = 11.84GINYY70 pKa = 6.45EE71 pKa = 3.94TKK73 pKa = 10.53ARR75 pKa = 11.84QQ76 pKa = 3.01

Molecular weight: 8.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

18455

37

1279

202.8

22.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.371 ± 0.426

1.138 ± 0.153

6.551 ± 0.206

5.484 ± 0.332

2.644 ± 0.126

8.979 ± 0.572

2.303 ± 0.217

4.151 ± 0.167

3.343 ± 0.193

7.808 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.117

2.931 ± 0.155

5.489 ± 0.269

3.392 ± 0.166

7.954 ± 0.423

5.121 ± 0.3

6.302 ± 0.203

7.613 ± 0.212

1.962 ± 0.125

2.287 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski