Amino acid dipepetide frequency for Mycobacterium phage AN3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.691AlaAla: 10.691 ± 1.102
0.569AlaCys: 0.569 ± 0.206
4.491AlaAsp: 4.491 ± 0.511
8.35AlaGlu: 8.35 ± 0.819
3.543AlaPhe: 3.543 ± 0.548
8.224AlaGly: 8.224 ± 0.923
1.835AlaHis: 1.835 ± 0.373
4.555AlaIle: 4.555 ± 0.638
4.365AlaLys: 4.365 ± 0.565
9.742AlaLeu: 9.742 ± 1.019
2.91AlaMet: 2.91 ± 0.427
2.973AlaAsn: 2.973 ± 0.527
4.997AlaPro: 4.997 ± 0.605
4.049AlaGln: 4.049 ± 0.671
7.022AlaArg: 7.022 ± 0.826
4.808AlaSer: 4.808 ± 0.469
5.693AlaThr: 5.693 ± 0.569
7.275AlaVal: 7.275 ± 0.675
1.581AlaTrp: 1.581 ± 0.296
2.53AlaTyr: 2.53 ± 0.432
0.0AlaXaa: 0.0 ± 0.0
Cys
0.696CysAla: 0.696 ± 0.171
0.127CysCys: 0.127 ± 0.132
0.633CysAsp: 0.633 ± 0.215
0.443CysGlu: 0.443 ± 0.158
0.253CysPhe: 0.253 ± 0.158
0.696CysGly: 0.696 ± 0.256
0.19CysHis: 0.19 ± 0.153
0.253CysIle: 0.253 ± 0.138
0.633CysLys: 0.633 ± 0.192
0.886CysLeu: 0.886 ± 0.234
0.253CysMet: 0.253 ± 0.165
0.506CysAsn: 0.506 ± 0.162
0.569CysPro: 0.569 ± 0.245
0.063CysGln: 0.063 ± 0.063
0.443CysArg: 0.443 ± 0.147
0.759CysSer: 0.759 ± 0.243
0.506CysThr: 0.506 ± 0.197
0.506CysVal: 0.506 ± 0.16
0.19CysTrp: 0.19 ± 0.097
0.569CysTyr: 0.569 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
6.516AspAla: 6.516 ± 0.833
0.759AspCys: 0.759 ± 0.204
3.796AspAsp: 3.796 ± 0.547
5.061AspGlu: 5.061 ± 0.82
2.657AspPhe: 2.657 ± 0.52
5.883AspGly: 5.883 ± 0.599
1.708AspHis: 1.708 ± 0.396
3.416AspIle: 3.416 ± 0.395
2.53AspLys: 2.53 ± 0.384
4.871AspLeu: 4.871 ± 0.592
1.961AspMet: 1.961 ± 0.322
1.581AspAsn: 1.581 ± 0.431
4.491AspPro: 4.491 ± 0.573
2.277AspGln: 2.277 ± 0.397
2.973AspArg: 2.973 ± 0.439
2.53AspSer: 2.53 ± 0.334
2.657AspThr: 2.657 ± 0.424
3.732AspVal: 3.732 ± 0.541
1.392AspTrp: 1.392 ± 0.319
1.898AspTyr: 1.898 ± 0.299
0.0AspXaa: 0.0 ± 0.0
Glu
7.528GluAla: 7.528 ± 0.819
0.253GluCys: 0.253 ± 0.134
5.314GluAsp: 5.314 ± 0.699
5.314GluGlu: 5.314 ± 0.843
3.226GluPhe: 3.226 ± 0.493
5.187GluGly: 5.187 ± 0.654
1.898GluHis: 1.898 ± 0.354
4.049GluIle: 4.049 ± 0.515
2.973GluLys: 2.973 ± 0.411
6.769GluLeu: 6.769 ± 0.707
2.214GluMet: 2.214 ± 0.34
2.214GluAsn: 2.214 ± 0.375
2.214GluPro: 2.214 ± 0.357
2.657GluGln: 2.657 ± 0.393
4.997GluArg: 4.997 ± 0.66
3.036GluSer: 3.036 ± 0.487
3.922GluThr: 3.922 ± 0.502
4.428GluVal: 4.428 ± 0.537
1.518GluTrp: 1.518 ± 0.325
1.835GluTyr: 1.835 ± 0.34
0.0GluXaa: 0.0 ± 0.0
Phe
3.289PheAla: 3.289 ± 0.499
0.316PheCys: 0.316 ± 0.137
2.72PheAsp: 2.72 ± 0.416
2.214PheGlu: 2.214 ± 0.36
0.822PhePhe: 0.822 ± 0.218
3.416PheGly: 3.416 ± 0.475
1.012PheHis: 1.012 ± 0.278
1.265PheIle: 1.265 ± 0.354
1.835PheLys: 1.835 ± 0.388
2.847PheLeu: 2.847 ± 0.479
0.696PheMet: 0.696 ± 0.217
1.392PheAsn: 1.392 ± 0.319
2.024PhePro: 2.024 ± 0.372
1.012PheGln: 1.012 ± 0.229
2.088PheArg: 2.088 ± 0.326
1.771PheSer: 1.771 ± 0.333
1.961PheThr: 1.961 ± 0.284
2.341PheVal: 2.341 ± 0.378
0.443PheTrp: 0.443 ± 0.159
1.075PheTyr: 1.075 ± 0.236
0.0PheXaa: 0.0 ± 0.0
Gly
7.338GlyAla: 7.338 ± 0.978
0.759GlyCys: 0.759 ± 0.211
5.63GlyAsp: 5.63 ± 0.627
5.693GlyGlu: 5.693 ± 0.699
3.416GlyPhe: 3.416 ± 0.524
8.54GlyGly: 8.54 ± 1.635
1.328GlyHis: 1.328 ± 0.264
4.428GlyIle: 4.428 ± 0.645
4.175GlyLys: 4.175 ± 0.557
7.148GlyLeu: 7.148 ± 0.729
1.708GlyMet: 1.708 ± 0.313
3.1GlyAsn: 3.1 ± 0.459
3.732GlyPro: 3.732 ± 0.408
3.226GlyGln: 3.226 ± 0.472
3.985GlyArg: 3.985 ± 0.453
4.365GlySer: 4.365 ± 0.599
5.124GlyThr: 5.124 ± 0.681
6.389GlyVal: 6.389 ± 0.688
1.265GlyTrp: 1.265 ± 0.227
2.404GlyTyr: 2.404 ± 0.327
0.0GlyXaa: 0.0 ± 0.0
His
1.581HisAla: 1.581 ± 0.307
0.316HisCys: 0.316 ± 0.14
1.392HisAsp: 1.392 ± 0.271
1.455HisGlu: 1.455 ± 0.366
0.38HisPhe: 0.38 ± 0.143
2.151HisGly: 2.151 ± 0.466
0.506HisHis: 0.506 ± 0.165
1.202HisIle: 1.202 ± 0.236
1.139HisLys: 1.139 ± 0.249
1.392HisLeu: 1.392 ± 0.302
0.38HisMet: 0.38 ± 0.148
0.633HisAsn: 0.633 ± 0.214
1.075HisPro: 1.075 ± 0.228
0.949HisGln: 0.949 ± 0.234
1.645HisArg: 1.645 ± 0.374
0.886HisSer: 0.886 ± 0.266
1.202HisThr: 1.202 ± 0.251
1.075HisVal: 1.075 ± 0.247
0.316HisTrp: 0.316 ± 0.116
0.38HisTyr: 0.38 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
5.946IleAla: 5.946 ± 0.502
0.696IleCys: 0.696 ± 0.203
3.669IleAsp: 3.669 ± 0.485
4.555IleGlu: 4.555 ± 0.507
1.581IlePhe: 1.581 ± 0.329
4.808IleGly: 4.808 ± 0.585
1.012IleHis: 1.012 ± 0.205
1.771IleIle: 1.771 ± 0.299
2.53IleLys: 2.53 ± 0.448
3.479IleLeu: 3.479 ± 0.47
0.696IleMet: 0.696 ± 0.162
2.088IleAsn: 2.088 ± 0.323
3.353IlePro: 3.353 ± 0.556
1.581IleGln: 1.581 ± 0.397
3.226IleArg: 3.226 ± 0.422
2.594IleSer: 2.594 ± 0.357
3.163IleThr: 3.163 ± 0.378
2.214IleVal: 2.214 ± 0.372
0.569IleTrp: 0.569 ± 0.169
1.012IleTyr: 1.012 ± 0.234
0.0IleXaa: 0.0 ± 0.0
Lys
5.124LysAla: 5.124 ± 0.622
0.316LysCys: 0.316 ± 0.149
3.036LysAsp: 3.036 ± 0.451
2.341LysGlu: 2.341 ± 0.34
1.265LysPhe: 1.265 ± 0.242
3.732LysGly: 3.732 ± 0.479
0.696LysHis: 0.696 ± 0.196
2.72LysIle: 2.72 ± 0.439
2.91LysLys: 2.91 ± 0.649
4.175LysLeu: 4.175 ± 0.476
1.139LysMet: 1.139 ± 0.277
1.392LysAsn: 1.392 ± 0.286
2.53LysPro: 2.53 ± 0.494
1.898LysGln: 1.898 ± 0.321
3.226LysArg: 3.226 ± 0.478
2.594LysSer: 2.594 ± 0.4
2.91LysThr: 2.91 ± 0.408
3.859LysVal: 3.859 ± 0.452
0.633LysTrp: 0.633 ± 0.203
1.328LysTyr: 1.328 ± 0.338
0.0LysXaa: 0.0 ± 0.0
Leu
8.54LeuAla: 8.54 ± 0.815
0.822LeuCys: 0.822 ± 0.227
5.377LeuAsp: 5.377 ± 0.697
6.263LeuGlu: 6.263 ± 0.625
2.72LeuPhe: 2.72 ± 0.348
5.44LeuGly: 5.44 ± 0.836
2.024LeuHis: 2.024 ± 0.341
4.112LeuIle: 4.112 ± 0.454
3.416LeuLys: 3.416 ± 0.426
6.326LeuLeu: 6.326 ± 0.628
2.657LeuMet: 2.657 ± 0.491
2.53LeuAsn: 2.53 ± 0.376
4.428LeuPro: 4.428 ± 0.519
3.036LeuGln: 3.036 ± 0.568
5.82LeuArg: 5.82 ± 0.657
5.377LeuSer: 5.377 ± 0.793
5.251LeuThr: 5.251 ± 0.717
5.504LeuVal: 5.504 ± 0.593
1.581LeuTrp: 1.581 ± 0.323
2.151LeuTyr: 2.151 ± 0.404
0.0LeuXaa: 0.0 ± 0.0
Met
2.973MetAla: 2.973 ± 0.481
0.063MetCys: 0.063 ± 0.063
1.328MetAsp: 1.328 ± 0.283
1.202MetGlu: 1.202 ± 0.283
0.633MetPhe: 0.633 ± 0.183
1.771MetGly: 1.771 ± 0.35
0.38MetHis: 0.38 ± 0.147
1.581MetIle: 1.581 ± 0.318
1.455MetLys: 1.455 ± 0.314
1.392MetLeu: 1.392 ± 0.292
0.506MetMet: 0.506 ± 0.182
0.633MetAsn: 0.633 ± 0.189
1.708MetPro: 1.708 ± 0.346
1.139MetGln: 1.139 ± 0.279
1.771MetArg: 1.771 ± 0.376
2.404MetSer: 2.404 ± 0.321
2.53MetThr: 2.53 ± 0.359
0.886MetVal: 0.886 ± 0.245
0.127MetTrp: 0.127 ± 0.086
0.696MetTyr: 0.696 ± 0.197
0.0MetXaa: 0.0 ± 0.0
Asn
2.72AsnAla: 2.72 ± 0.446
0.38AsnCys: 0.38 ± 0.151
2.088AsnAsp: 2.088 ± 0.378
2.214AsnGlu: 2.214 ± 0.388
0.759AsnPhe: 0.759 ± 0.208
3.163AsnGly: 3.163 ± 0.453
0.759AsnHis: 0.759 ± 0.192
0.949AsnIle: 0.949 ± 0.281
1.139AsnLys: 1.139 ± 0.236
3.479AsnLeu: 3.479 ± 0.45
0.696AsnMet: 0.696 ± 0.191
0.633AsnAsn: 0.633 ± 0.203
2.467AsnPro: 2.467 ± 0.38
0.696AsnGln: 0.696 ± 0.198
2.151AsnArg: 2.151 ± 0.365
1.455AsnSer: 1.455 ± 0.251
2.151AsnThr: 2.151 ± 0.457
1.961AsnVal: 1.961 ± 0.313
0.886AsnTrp: 0.886 ± 0.214
0.949AsnTyr: 0.949 ± 0.22
0.0AsnXaa: 0.0 ± 0.0
Pro
4.871ProAla: 4.871 ± 0.514
0.316ProCys: 0.316 ± 0.149
4.302ProAsp: 4.302 ± 0.545
5.187ProGlu: 5.187 ± 0.634
1.708ProPhe: 1.708 ± 0.366
4.681ProGly: 4.681 ± 0.567
0.886ProHis: 0.886 ± 0.244
2.53ProIle: 2.53 ± 0.377
2.341ProLys: 2.341 ± 0.474
3.353ProLeu: 3.353 ± 0.484
1.265ProMet: 1.265 ± 0.267
1.835ProAsn: 1.835 ± 0.333
2.088ProPro: 2.088 ± 0.418
1.645ProGln: 1.645 ± 0.344
3.543ProArg: 3.543 ± 0.489
2.53ProSer: 2.53 ± 0.365
2.973ProThr: 2.973 ± 0.505
3.859ProVal: 3.859 ± 0.517
1.265ProTrp: 1.265 ± 0.349
1.455ProTyr: 1.455 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
4.428GlnAla: 4.428 ± 0.617
0.19GlnCys: 0.19 ± 0.102
1.328GlnAsp: 1.328 ± 0.281
1.708GlnGlu: 1.708 ± 0.332
1.328GlnPhe: 1.328 ± 0.271
2.783GlnGly: 2.783 ± 0.458
0.443GlnHis: 0.443 ± 0.158
3.1GlnIle: 3.1 ± 0.387
1.835GlnLys: 1.835 ± 0.34
3.416GlnLeu: 3.416 ± 0.698
1.075GlnMet: 1.075 ± 0.221
0.696GlnAsn: 0.696 ± 0.2
1.202GlnPro: 1.202 ± 0.287
2.214GlnGln: 2.214 ± 0.384
2.404GlnArg: 2.404 ± 0.469
1.581GlnSer: 1.581 ± 0.322
2.277GlnThr: 2.277 ± 0.434
3.1GlnVal: 3.1 ± 0.371
0.759GlnTrp: 0.759 ± 0.238
1.012GlnTyr: 1.012 ± 0.208
0.0GlnXaa: 0.0 ± 0.0
Arg
5.187ArgAla: 5.187 ± 0.761
1.139ArgCys: 1.139 ± 0.288
4.112ArgAsp: 4.112 ± 0.514
4.934ArgGlu: 4.934 ± 0.678
2.341ArgPhe: 2.341 ± 0.381
4.112ArgGly: 4.112 ± 0.452
1.328ArgHis: 1.328 ± 0.331
3.416ArgIle: 3.416 ± 0.37
3.796ArgLys: 3.796 ± 0.45
5.377ArgLeu: 5.377 ± 0.616
1.708ArgMet: 1.708 ± 0.355
2.214ArgAsn: 2.214 ± 0.365
3.1ArgPro: 3.1 ± 0.406
2.151ArgGln: 2.151 ± 0.379
5.061ArgArg: 5.061 ± 0.758
3.036ArgSer: 3.036 ± 0.516
3.163ArgThr: 3.163 ± 0.496
4.491ArgVal: 4.491 ± 0.575
1.392ArgTrp: 1.392 ± 0.282
2.467ArgTyr: 2.467 ± 0.374
0.0ArgXaa: 0.0 ± 0.0
Ser
5.251SerAla: 5.251 ± 0.694
0.443SerCys: 0.443 ± 0.161
3.036SerAsp: 3.036 ± 0.42
3.163SerGlu: 3.163 ± 0.457
1.898SerPhe: 1.898 ± 0.404
4.871SerGly: 4.871 ± 0.469
0.949SerHis: 0.949 ± 0.24
2.024SerIle: 2.024 ± 0.302
3.036SerLys: 3.036 ± 0.613
4.049SerLeu: 4.049 ± 0.585
1.392SerMet: 1.392 ± 0.32
1.139SerAsn: 1.139 ± 0.247
2.783SerPro: 2.783 ± 0.406
2.088SerGln: 2.088 ± 0.335
3.226SerArg: 3.226 ± 0.432
2.594SerSer: 2.594 ± 0.466
3.353SerThr: 3.353 ± 0.439
3.416SerVal: 3.416 ± 0.554
1.075SerTrp: 1.075 ± 0.313
1.455SerTyr: 1.455 ± 0.335
0.0SerXaa: 0.0 ± 0.0
Thr
6.389ThrAla: 6.389 ± 0.64
0.443ThrCys: 0.443 ± 0.17
3.1ThrAsp: 3.1 ± 0.531
3.416ThrGlu: 3.416 ± 0.391
1.835ThrPhe: 1.835 ± 0.306
5.187ThrGly: 5.187 ± 0.736
1.139ThrHis: 1.139 ± 0.262
2.91ThrIle: 2.91 ± 0.47
2.847ThrLys: 2.847 ± 0.49
5.314ThrLeu: 5.314 ± 0.57
1.328ThrMet: 1.328 ± 0.293
1.392ThrAsn: 1.392 ± 0.265
4.555ThrPro: 4.555 ± 0.687
1.771ThrGln: 1.771 ± 0.346
3.289ThrArg: 3.289 ± 0.455
2.467ThrSer: 2.467 ± 0.401
2.91ThrThr: 2.91 ± 0.513
5.377ThrVal: 5.377 ± 0.665
1.202ThrTrp: 1.202 ± 0.26
1.835ThrTyr: 1.835 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
6.326ValAla: 6.326 ± 0.86
0.506ValCys: 0.506 ± 0.163
4.491ValAsp: 4.491 ± 0.515
4.744ValGlu: 4.744 ± 0.546
2.467ValPhe: 2.467 ± 0.437
4.997ValGly: 4.997 ± 0.59
1.202ValHis: 1.202 ± 0.247
3.543ValIle: 3.543 ± 0.464
3.732ValLys: 3.732 ± 0.372
5.883ValLeu: 5.883 ± 0.737
1.392ValMet: 1.392 ± 0.302
3.036ValAsn: 3.036 ± 0.457
2.847ValPro: 2.847 ± 0.463
2.151ValGln: 2.151 ± 0.362
4.618ValArg: 4.618 ± 0.553
3.985ValSer: 3.985 ± 0.559
4.302ValThr: 4.302 ± 0.412
4.934ValVal: 4.934 ± 0.523
1.328ValTrp: 1.328 ± 0.294
2.024ValTyr: 2.024 ± 0.4
0.0ValXaa: 0.0 ± 0.0
Trp
1.708TrpAla: 1.708 ± 0.321
0.38TrpCys: 0.38 ± 0.171
0.949TrpAsp: 0.949 ± 0.264
1.328TrpGlu: 1.328 ± 0.275
0.696TrpPhe: 0.696 ± 0.223
1.581TrpGly: 1.581 ± 0.321
0.38TrpHis: 0.38 ± 0.151
1.012TrpIle: 1.012 ± 0.245
0.443TrpLys: 0.443 ± 0.181
1.328TrpLeu: 1.328 ± 0.288
0.506TrpMet: 0.506 ± 0.172
0.696TrpAsn: 0.696 ± 0.206
1.012TrpPro: 1.012 ± 0.232
1.139TrpGln: 1.139 ± 0.241
1.328TrpArg: 1.328 ± 0.271
0.886TrpSer: 0.886 ± 0.257
1.075TrpThr: 1.075 ± 0.267
1.139TrpVal: 1.139 ± 0.253
0.506TrpTrp: 0.506 ± 0.194
0.506TrpTyr: 0.506 ± 0.177
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.1TyrAla: 3.1 ± 0.431
0.38TyrCys: 0.38 ± 0.142
1.898TyrAsp: 1.898 ± 0.28
1.961TyrGlu: 1.961 ± 0.312
1.012TyrPhe: 1.012 ± 0.208
2.53TyrGly: 2.53 ± 0.397
0.38TyrHis: 0.38 ± 0.135
1.708TyrIle: 1.708 ± 0.29
0.759TyrLys: 0.759 ± 0.197
2.151TyrLeu: 2.151 ± 0.341
0.633TyrMet: 0.633 ± 0.196
1.012TyrAsn: 1.012 ± 0.242
1.455TyrPro: 1.455 ± 0.272
1.139TyrGln: 1.139 ± 0.25
1.708TyrArg: 1.708 ± 0.372
1.581TyrSer: 1.581 ± 0.378
1.581TyrThr: 1.581 ± 0.275
2.024TyrVal: 2.024 ± 0.385
0.569TyrTrp: 0.569 ± 0.206
0.759TyrTyr: 0.759 ± 0.218
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (15809 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski