Amino acid dipepetide frequency for gamma proteobacterium HTCC5015

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.009AlaAla: 10.009 ± 0.166	1.113AlaCys: 1.113 ± 0.035	5.624AlaAsp: 5.624 ± 0.096	6.738AlaGlu: 6.738 ± 0.121	3.492AlaPhe: 3.492 ± 0.076	6.86AlaGly: 6.86 ± 0.107	2.091AlaHis: 2.091 ± 0.058	5.405AlaIle: 5.405 ± 0.087	4.073AlaLys: 4.073 ± 0.096	11.546AlaLeu: 11.546 ± 0.15	2.863AlaMet: 2.863 ± 0.057	2.971AlaAsn: 2.971 ± 0.064	3.819AlaPro: 3.819 ± 0.072	4.655AlaGln: 4.655 ± 0.091	5.346AlaArg: 5.346 ± 0.097	5.997AlaSer: 5.997 ± 0.095	4.532AlaThr: 4.532 ± 0.078	7.266AlaVal: 7.266 ± 0.11	1.249AlaTrp: 1.249 ± 0.045	2.459AlaTyr: 2.459 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.912CysAla: 0.912 ± 0.034	0.135CysCys: 0.135 ± 0.015	0.662CysAsp: 0.662 ± 0.031	0.651CysGlu: 0.651 ± 0.032	0.381CysPhe: 0.381 ± 0.027	0.909CysGly: 0.909 ± 0.038	0.337CysHis: 0.337 ± 0.023	0.454CysIle: 0.454 ± 0.024	0.25CysLys: 0.25 ± 0.017	1.0CysLeu: 1.0 ± 0.031	0.153CysMet: 0.153 ± 0.015	0.296CysAsn: 0.296 ± 0.02	0.472CysPro: 0.472 ± 0.032	0.455CysGln: 0.455 ± 0.025	0.553CysArg: 0.553 ± 0.025	0.568CysSer: 0.568 ± 0.03	0.435CysThr: 0.435 ± 0.027	0.671CysVal: 0.671 ± 0.031	0.119CysTrp: 0.119 ± 0.015	0.298CysTyr: 0.298 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	5.442AspAla: 5.442 ± 0.101	0.556AspCys: 0.556 ± 0.028	3.66AspAsp: 3.66 ± 0.085	4.102AspGlu: 4.102 ± 0.077	2.563AspPhe: 2.563 ± 0.07	4.429AspGly: 4.429 ± 0.106	1.338AspHis: 1.338 ± 0.039	3.835AspIle: 3.835 ± 0.074	2.411AspLys: 2.411 ± 0.064	5.662AspLeu: 5.662 ± 0.084	1.416AspMet: 1.416 ± 0.047	2.12AspAsn: 2.12 ± 0.062	2.513AspPro: 2.513 ± 0.061	2.441AspGln: 2.441 ± 0.065	3.29AspArg: 3.29 ± 0.07	3.663AspSer: 3.663 ± 0.076	2.885AspThr: 2.885 ± 0.067	4.11AspVal: 4.11 ± 0.088	0.988AspTrp: 0.988 ± 0.041	2.154AspTyr: 2.154 ± 0.051	0.001AspXaa: 0.001 ± 0.001
Glu	7.37GluAla: 7.37 ± 0.118	0.523GluCys: 0.523 ± 0.027	3.259GluAsp: 3.259 ± 0.079	4.27GluGlu: 4.27 ± 0.12	2.142GluPhe: 2.142 ± 0.053	4.708GluGly: 4.708 ± 0.088	1.958GluHis: 1.958 ± 0.05	3.139GluIle: 3.139 ± 0.064	3.217GluLys: 3.217 ± 0.084	6.906GluLeu: 6.906 ± 0.12	1.666GluMet: 1.666 ± 0.054	2.195GluAsn: 2.195 ± 0.062	2.518GluPro: 2.518 ± 0.068	3.939GluGln: 3.939 ± 0.085	4.977GluArg: 4.977 ± 0.094	3.904GluSer: 3.904 ± 0.071	3.028GluThr: 3.028 ± 0.067	4.58GluVal: 4.58 ± 0.08	0.909GluTrp: 0.909 ± 0.035	1.749GluTyr: 1.749 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.21PheAla: 3.21 ± 0.073	0.413PheCys: 0.413 ± 0.024	2.687PheAsp: 2.687 ± 0.058	2.658PheGlu: 2.658 ± 0.061	1.6PhePhe: 1.6 ± 0.059	2.916PheGly: 2.916 ± 0.058	0.757PheHis: 0.757 ± 0.037	1.944PheIle: 1.944 ± 0.055	1.535PheLys: 1.535 ± 0.046	3.178PheLeu: 3.178 ± 0.078	0.945PheMet: 0.945 ± 0.032	1.542PheAsn: 1.542 ± 0.051	1.436PhePro: 1.436 ± 0.04	1.33PheGln: 1.33 ± 0.037	1.778PheArg: 1.778 ± 0.047	2.766PheSer: 2.766 ± 0.069	1.867PheThr: 1.867 ± 0.058	2.57PheVal: 2.57 ± 0.063	0.512PheTrp: 0.512 ± 0.026	1.221PheTyr: 1.221 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	6.543GlyAla: 6.543 ± 0.097	0.815GlyCys: 0.815 ± 0.038	4.709GlyAsp: 4.709 ± 0.095	5.266GlyGlu: 5.266 ± 0.093	3.206GlyPhe: 3.206 ± 0.065	5.796GlyGly: 5.796 ± 0.14	1.802GlyHis: 1.802 ± 0.054	4.098GlyIle: 4.098 ± 0.075	3.312GlyLys: 3.312 ± 0.076	7.671GlyLeu: 7.671 ± 0.114	1.933GlyMet: 1.933 ± 0.058	2.324GlyAsn: 2.324 ± 0.074	2.04GlyPro: 2.04 ± 0.056	3.362GlyGln: 3.362 ± 0.071	4.252GlyArg: 4.252 ± 0.082	4.348GlySer: 4.348 ± 0.097	3.571GlyThr: 3.571 ± 0.082	5.872GlyVal: 5.872 ± 0.087	1.037GlyTrp: 1.037 ± 0.042	2.378GlyTyr: 2.378 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.984HisAla: 1.984 ± 0.055	0.329HisCys: 0.329 ± 0.022	1.154HisAsp: 1.154 ± 0.04	1.231HisGlu: 1.231 ± 0.041	1.239HisPhe: 1.239 ± 0.05	1.804HisGly: 1.804 ± 0.058	0.828HisHis: 0.828 ± 0.038	1.426HisIle: 1.426 ± 0.048	0.853HisLys: 0.853 ± 0.035	2.502HisLeu: 2.502 ± 0.066	0.578HisMet: 0.578 ± 0.027	0.707HisAsn: 0.707 ± 0.031	1.322HisPro: 1.322 ± 0.037	1.15HisGln: 1.15 ± 0.043	1.571HisArg: 1.571 ± 0.045	1.636HisSer: 1.636 ± 0.054	1.208HisThr: 1.208 ± 0.044	1.246HisVal: 1.246 ± 0.043	0.475HisTrp: 0.475 ± 0.026	1.019HisTyr: 1.019 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.73IleAla: 5.73 ± 0.087	0.471IleCys: 0.471 ± 0.025	4.08IleAsp: 4.08 ± 0.075	4.467IleGlu: 4.467 ± 0.084	1.626IlePhe: 1.626 ± 0.048	4.31IleGly: 4.31 ± 0.079	1.241IleHis: 1.241 ± 0.053	2.338IleIle: 2.338 ± 0.06	2.435IleLys: 2.435 ± 0.06	4.008IleLeu: 4.008 ± 0.079	1.027IleMet: 1.027 ± 0.037	2.023IleAsn: 2.023 ± 0.053	2.317IlePro: 2.317 ± 0.054	2.174IleGln: 2.174 ± 0.054	3.004IleArg: 3.004 ± 0.054	3.024IleSer: 3.024 ± 0.071	2.822IleThr: 2.822 ± 0.067	3.536IleVal: 3.536 ± 0.072	0.447IleTrp: 0.447 ± 0.027	1.393IleTyr: 1.393 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	4.752LysAla: 4.752 ± 0.112	0.193LysCys: 0.193 ± 0.019	2.251LysAsp: 2.251 ± 0.051	2.481LysGlu: 2.481 ± 0.071	0.868LysPhe: 0.868 ± 0.038	2.981LysGly: 2.981 ± 0.07	1.014LysHis: 1.014 ± 0.039	2.084LysIle: 2.084 ± 0.06	2.497LysLys: 2.497 ± 0.139	4.054LysLeu: 4.054 ± 0.079	1.085LysMet: 1.085 ± 0.039	1.573LysAsn: 1.573 ± 0.049	2.122LysPro: 2.122 ± 0.058	2.05LysGln: 2.05 ± 0.06	3.012LysArg: 3.012 ± 0.064	2.518LysSer: 2.518 ± 0.069	2.482LysThr: 2.482 ± 0.065	2.882LysVal: 2.882 ± 0.076	0.371LysTrp: 0.371 ± 0.025	0.881LysTyr: 0.881 ± 0.038	0.001LysXaa: 0.001 ± 0.002
Leu	10.386LeuAla: 10.386 ± 0.144	1.122LeuCys: 1.122 ± 0.044	6.338LeuAsp: 6.338 ± 0.108	6.885LeuGlu: 6.885 ± 0.109	3.741LeuPhe: 3.741 ± 0.081	7.795LeuGly: 7.795 ± 0.131	2.084LeuHis: 2.084 ± 0.052	5.178LeuIle: 5.178 ± 0.084	4.958LeuLys: 4.958 ± 0.085	10.185LeuLeu: 10.185 ± 0.157	2.644LeuMet: 2.644 ± 0.066	3.898LeuAsn: 3.898 ± 0.074	5.097LeuPro: 5.097 ± 0.092	3.865LeuGln: 3.865 ± 0.072	5.87LeuArg: 5.87 ± 0.079	7.757LeuSer: 7.757 ± 0.099	4.852LeuThr: 4.852 ± 0.085	6.707LeuVal: 6.707 ± 0.107	1.291LeuTrp: 1.291 ± 0.047	2.65LeuTyr: 2.65 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.759MetAla: 2.759 ± 0.062	0.184MetCys: 0.184 ± 0.016	1.332MetAsp: 1.332 ± 0.044	1.29MetGlu: 1.29 ± 0.041	0.656MetPhe: 0.656 ± 0.032	2.054MetGly: 2.054 ± 0.057	0.539MetHis: 0.539 ± 0.026	1.15MetIle: 1.15 ± 0.038	1.22MetLys: 1.22 ± 0.039	2.48MetLeu: 2.48 ± 0.058	0.662MetMet: 0.662 ± 0.032	0.946MetAsn: 0.946 ± 0.033	1.258MetPro: 1.258 ± 0.039	1.122MetGln: 1.122 ± 0.041	1.453MetArg: 1.453 ± 0.044	1.937MetSer: 1.937 ± 0.059	1.426MetThr: 1.426 ± 0.046	1.759MetVal: 1.759 ± 0.046	0.222MetTrp: 0.222 ± 0.018	0.447MetTyr: 0.447 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.346AsnAla: 3.346 ± 0.07	0.295AsnCys: 0.295 ± 0.021	1.895AsnAsp: 1.895 ± 0.063	1.797AsnGlu: 1.797 ± 0.053	1.179AsnPhe: 1.179 ± 0.044	2.709AsnGly: 2.709 ± 0.077	0.891AsnHis: 0.891 ± 0.036	2.07AsnIle: 2.07 ± 0.06	1.325AsnLys: 1.325 ± 0.045	3.108AsnLeu: 3.108 ± 0.067	0.781AsnMet: 0.781 ± 0.03	1.293AsnAsn: 1.293 ± 0.05	1.94AsnPro: 1.94 ± 0.052	1.474AsnGln: 1.474 ± 0.047	2.222AsnArg: 2.222 ± 0.062	1.932AsnSer: 1.932 ± 0.052	2.011AsnThr: 2.011 ± 0.057	1.987AsnVal: 1.987 ± 0.054	0.48AsnTrp: 0.48 ± 0.026	0.937AsnTyr: 0.937 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	3.647ProAla: 3.647 ± 0.063	0.363ProCys: 0.363 ± 0.024	2.764ProAsp: 2.764 ± 0.06	3.77ProGlu: 3.77 ± 0.083	1.642ProPhe: 1.642 ± 0.049	2.62ProGly: 2.62 ± 0.063	1.024ProHis: 1.024 ± 0.034	2.271ProIle: 2.271 ± 0.057	1.851ProLys: 1.851 ± 0.056	4.463ProLeu: 4.463 ± 0.083	1.175ProMet: 1.175 ± 0.046	1.559ProAsn: 1.559 ± 0.047	1.662ProPro: 1.662 ± 0.057	1.98ProGln: 1.98 ± 0.054	2.097ProArg: 2.097 ± 0.057	2.965ProSer: 2.965 ± 0.069	2.093ProThr: 2.093 ± 0.058	3.282ProVal: 3.282 ± 0.063	0.623ProTrp: 0.623 ± 0.033	1.151ProTyr: 1.151 ± 0.04	0.001ProXaa: 0.001 ± 0.001
Gln	5.019GlnAla: 5.019 ± 0.089	0.458GlnCys: 0.458 ± 0.025	1.85GlnAsp: 1.85 ± 0.05	2.44GlnGlu: 2.44 ± 0.063	1.663GlnPhe: 1.663 ± 0.051	3.178GlnGly: 3.178 ± 0.065	1.393GlnHis: 1.393 ± 0.045	2.01GlnIle: 2.01 ± 0.048	1.835GlnLys: 1.835 ± 0.046	5.093GlnLeu: 5.093 ± 0.098	1.07GlnMet: 1.07 ± 0.035	1.334GlnAsn: 1.334 ± 0.045	1.845GlnPro: 1.845 ± 0.045	2.846GlnGln: 2.846 ± 0.086	3.9GlnArg: 3.9 ± 0.084	2.992GlnSer: 2.992 ± 0.067	2.06GlnThr: 2.06 ± 0.058	3.061GlnVal: 3.061 ± 0.065	0.896GlnTrp: 0.896 ± 0.045	1.236GlnTyr: 1.236 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	5.236ArgAla: 5.236 ± 0.09	0.599ArgCys: 0.599 ± 0.03	3.57ArgAsp: 3.57 ± 0.07	4.384ArgGlu: 4.384 ± 0.077	2.695ArgPhe: 2.695 ± 0.06	3.877ArgGly: 3.877 ± 0.075	1.735ArgHis: 1.735 ± 0.052	3.391ArgIle: 3.391 ± 0.078	2.263ArgLys: 2.263 ± 0.055	6.587ArgLeu: 6.587 ± 0.106	1.441ArgMet: 1.441 ± 0.041	1.874ArgAsn: 1.874 ± 0.058	2.3ArgPro: 2.3 ± 0.056	3.288ArgGln: 3.288 ± 0.073	3.982ArgArg: 3.982 ± 0.088	3.407ArgSer: 3.407 ± 0.07	2.271ArgThr: 2.271 ± 0.055	4.168ArgVal: 4.168 ± 0.081	1.073ArgTrp: 1.073 ± 0.042	2.391ArgTyr: 2.391 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	6.365SerAla: 6.365 ± 0.095	0.568SerCys: 0.568 ± 0.031	4.188SerAsp: 4.188 ± 0.098	4.31SerGlu: 4.31 ± 0.081	2.256SerPhe: 2.256 ± 0.059	5.417SerGly: 5.417 ± 0.089	1.52SerHis: 1.52 ± 0.038	3.114SerIle: 3.114 ± 0.065	2.387SerLys: 2.387 ± 0.063	6.452SerLeu: 6.452 ± 0.096	1.515SerMet: 1.515 ± 0.044	2.015SerAsn: 2.015 ± 0.054	2.632SerPro: 2.632 ± 0.06	2.887SerGln: 2.887 ± 0.063	3.477SerArg: 3.477 ± 0.066	3.877SerSer: 3.877 ± 0.083	2.996SerThr: 2.996 ± 0.077	4.635SerVal: 4.635 ± 0.084	0.72SerTrp: 0.72 ± 0.033	1.636SerTyr: 1.636 ± 0.05	0.001SerXaa: 0.001 ± 0.001
Thr	4.932ThrAla: 4.932 ± 0.077	0.388ThrCys: 0.388 ± 0.023	2.64ThrAsp: 2.64 ± 0.07	2.844ThrGlu: 2.844 ± 0.067	1.596ThrPhe: 1.596 ± 0.053	3.926ThrGly: 3.926 ± 0.084	1.199ThrHis: 1.199 ± 0.035	2.514ThrIle: 2.514 ± 0.063	1.539ThrLys: 1.539 ± 0.052	6.166ThrLeu: 6.166 ± 0.091	1.032ThrMet: 1.032 ± 0.037	1.35ThrAsn: 1.35 ± 0.047	2.787ThrPro: 2.787 ± 0.066	2.281ThrGln: 2.281 ± 0.052	2.58ThrArg: 2.58 ± 0.062	2.713ThrSer: 2.713 ± 0.065	2.563ThrThr: 2.563 ± 0.073	3.852ThrVal: 3.852 ± 0.075	0.554ThrTrp: 0.554 ± 0.026	1.205ThrTyr: 1.205 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	7.077ValAla: 7.077 ± 0.111	0.688ValCys: 0.688 ± 0.022	4.589ValAsp: 4.589 ± 0.081	5.008ValGlu: 5.008 ± 0.086	2.594ValPhe: 2.594 ± 0.078	4.991ValGly: 4.991 ± 0.09	1.383ValHis: 1.383 ± 0.048	4.012ValIle: 4.012 ± 0.081	2.742ValLys: 2.742 ± 0.078	7.311ValLeu: 7.311 ± 0.109	1.923ValMet: 1.923 ± 0.058	2.391ValAsn: 2.391 ± 0.062	2.969ValPro: 2.969 ± 0.06	2.398ValGln: 2.398 ± 0.057	3.902ValArg: 3.902 ± 0.074	4.507ValSer: 4.507 ± 0.078	3.612ValThr: 3.612 ± 0.073	5.694ValVal: 5.694 ± 0.123	0.846ValTrp: 0.846 ± 0.037	1.808ValTyr: 1.808 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.929TrpAla: 0.929 ± 0.036	0.16TrpCys: 0.16 ± 0.015	0.654TrpAsp: 0.654 ± 0.031	0.639TrpGlu: 0.639 ± 0.03	0.513TrpPhe: 0.513 ± 0.03	0.904TrpGly: 0.904 ± 0.04	0.396TrpHis: 0.396 ± 0.025	0.613TrpIle: 0.613 ± 0.031	0.453TrpLys: 0.453 ± 0.026	2.044TrpLeu: 2.044 ± 0.061	0.413TrpMet: 0.413 ± 0.025	0.369TrpAsn: 0.369 ± 0.024	0.623TrpPro: 0.623 ± 0.032	0.876TrpGln: 0.876 ± 0.038	1.081TrpArg: 1.081 ± 0.044	0.749TrpSer: 0.749 ± 0.035	0.488TrpThr: 0.488 ± 0.03	1.008TrpVal: 1.008 ± 0.042	0.253TrpTrp: 0.253 ± 0.019	0.374TrpTyr: 0.374 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.459TyrAla: 2.459 ± 0.059	0.339TyrCys: 0.339 ± 0.022	1.673TyrAsp: 1.673 ± 0.057	1.725TyrGlu: 1.725 ± 0.056	1.212TyrPhe: 1.212 ± 0.042	2.154TyrGly: 2.154 ± 0.055	0.732TyrHis: 0.732 ± 0.032	1.428TyrIle: 1.428 ± 0.044	0.998TyrLys: 0.998 ± 0.043	2.886TyrLeu: 2.886 ± 0.057	0.573TyrMet: 0.573 ± 0.027	0.892TyrAsn: 0.892 ± 0.036	1.331TyrPro: 1.331 ± 0.047	1.605TyrGln: 1.605 ± 0.05	2.187TyrArg: 2.187 ± 0.049	1.71TyrSer: 1.71 ± 0.053	1.456TyrThr: 1.456 ± 0.048	1.557TyrVal: 1.557 ± 0.043	0.472TyrTrp: 0.472 ± 0.027	0.933TyrTyr: 0.933 ± 0.04	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.033XaaXaa: 0.033 ± 0.017

Statistics based on 2449 proteins (755730 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---