Spirochaeta lutea

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Spirochaeta

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2285 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A098QZ15|A0A098QZ15_9SPIO Uncharacterized protein OS=Spirochaeta lutea OX=1480694 GN=DC28_10850 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 9.06KK3 pKa = 7.89TLGVIAVLLLLVGLLAGCDD22 pKa = 3.33GMVIIGNDD30 pKa = 3.53LAGHH34 pKa = 5.0TWEE37 pKa = 4.81GRR39 pKa = 11.84VDD41 pKa = 3.75VLGLAGADD49 pKa = 3.39YY50 pKa = 10.93EE51 pKa = 5.14LRR53 pKa = 11.84FLTASTGEE61 pKa = 3.94YY62 pKa = 9.07WEE64 pKa = 4.15QVDD67 pKa = 3.8VLGGLGVAQAEE78 pKa = 4.74TTDD81 pKa = 3.72FTYY84 pKa = 10.83EE85 pKa = 3.97FDD87 pKa = 5.16PIEE90 pKa = 4.11MTGSIDD96 pKa = 5.07FSDD99 pKa = 3.49GDD101 pKa = 3.56YY102 pKa = 8.2WTFKK106 pKa = 10.72IEE108 pKa = 4.24TNNDD112 pKa = 3.51LTWTDD117 pKa = 2.34SGYY120 pKa = 11.25NYY122 pKa = 8.28TATYY126 pKa = 9.52KK127 pKa = 10.84

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A098R1T2|A0A098R1T2_9SPIO Uncharacterized protein OS=Spirochaeta lutea OX=1480694 GN=DC28_06365 PE=4 SV=1
MM1 pKa = 7.42LLSAAGLGGFILIWEE16 pKa = 4.5LSGRR20 pKa = 11.84AGLLPGTLIPLPSQVPQALARR41 pKa = 11.84EE42 pKa = 4.13MALPGIGLPGGLWMAKK58 pKa = 8.7VLQSLGHH65 pKa = 5.37YY66 pKa = 10.08LRR68 pKa = 11.84GVLGGIGAGVILGLTAGSLPKK89 pKa = 10.3LAALLQGLVRR99 pKa = 11.84LLRR102 pKa = 11.84PIPPLAWIPFAIIWFGITPAASAFIIFVGVFWINFLSTLGTVQHH146 pKa = 6.55SNPDD150 pKa = 3.43FRR152 pKa = 11.84EE153 pKa = 4.02VAHH156 pKa = 5.98VFGFGRR162 pKa = 11.84FLPRR166 pKa = 11.84LLHH169 pKa = 4.28VTIPEE174 pKa = 4.31SLPGMLTGIRR184 pKa = 11.84TSLGMGWMAVVASEE198 pKa = 4.52LFGIPGIGEE207 pKa = 4.27RR208 pKa = 11.84MNQAAGLLATDD219 pKa = 3.66IVLVYY224 pKa = 9.43MLTIAGLYY232 pKa = 9.4TLFDD236 pKa = 3.84SAFSLIQRR244 pKa = 11.84RR245 pKa = 11.84LTRR248 pKa = 11.84WLPKK252 pKa = 9.88EE253 pKa = 3.85

Molecular weight: 27.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2285

0

2285

806388

51

3356

352.9

39.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.843 ± 0.048

0.659 ± 0.015

5.399 ± 0.037

6.667 ± 0.046

4.188 ± 0.035

8.196 ± 0.053

1.942 ± 0.026

6.517 ± 0.044

3.744 ± 0.051

10.788 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.023

3.322 ± 0.032

4.955 ± 0.044

4.405 ± 0.043

6.121 ± 0.046

6.698 ± 0.046

5.477 ± 0.05

6.588 ± 0.041

1.198 ± 0.019

3.075 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski