Thrips-associated genomovirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; unclassified Genomoviridae

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8YT76|A0A1P8YT76_9VIRU RepA OS=Thrips-associated genomovirus 2 OX=1941234 PE=3 SV=1
MM1 pKa = 7.47TFLFSARR8 pKa = 11.84YY9 pKa = 9.42VLLTYY14 pKa = 9.1AQCGTLDD21 pKa = 3.17GWAVSDD27 pKa = 4.02HH28 pKa = 6.52LSALGAEE35 pKa = 4.64CIVGRR40 pKa = 11.84EE41 pKa = 4.0NHH43 pKa = 6.3SDD45 pKa = 3.41GGTHH49 pKa = 6.32LHH51 pKa = 6.56AFVDD55 pKa = 4.89FGRR58 pKa = 11.84KK59 pKa = 8.11KK60 pKa = 9.9QSRR63 pKa = 11.84RR64 pKa = 11.84PDD66 pKa = 3.25FFDD69 pKa = 4.06VGGHH73 pKa = 6.35HH74 pKa = 7.14PNIAPSRR81 pKa = 11.84GRR83 pKa = 11.84PEE85 pKa = 3.88RR86 pKa = 11.84GYY88 pKa = 11.11DD89 pKa = 3.48YY90 pKa = 10.71AIKK93 pKa = 10.87DD94 pKa = 3.59GDD96 pKa = 4.02VVAGGLARR104 pKa = 11.84PGGGGLPEE112 pKa = 4.34VANKK116 pKa = 9.25WSEE119 pKa = 3.81IVAAEE124 pKa = 4.05SRR126 pKa = 11.84DD127 pKa = 3.63EE128 pKa = 4.87FFDD131 pKa = 5.05LLRR134 pKa = 11.84QLDD137 pKa = 4.12PKK139 pKa = 10.46TLVTRR144 pKa = 11.84WTEE147 pKa = 3.7LNKK150 pKa = 10.8YY151 pKa = 10.14ADD153 pKa = 3.7AAYY156 pKa = 10.25APTPEE161 pKa = 5.03PYY163 pKa = 10.25VGSDD167 pKa = 2.91GKK169 pKa = 10.54QFEE172 pKa = 4.79LGMVPEE178 pKa = 4.41LARR181 pKa = 11.84WGEE184 pKa = 3.83QLVANDD190 pKa = 4.69PIEE193 pKa = 4.03GRR195 pKa = 11.84MRR197 pKa = 11.84SLVLYY202 pKa = 10.3GPSRR206 pKa = 11.84LGKK209 pKa = 8.13TIWARR214 pKa = 11.84SLGPHH219 pKa = 6.3VYY221 pKa = 11.09SMGIVSGKK229 pKa = 10.23LLLRR233 pKa = 11.84DD234 pKa = 3.77APGAAYY240 pKa = 9.98AVFDD244 pKa = 4.61DD245 pKa = 3.64MRR247 pKa = 11.84GGIGYY252 pKa = 7.9FHH254 pKa = 7.09SWKK257 pKa = 9.79EE258 pKa = 3.68WLGAQSVVTVKK269 pKa = 10.33EE270 pKa = 4.2LYY272 pKa = 9.8RR273 pKa = 11.84DD274 pKa = 3.61PVQLVWGRR282 pKa = 11.84PCIWLANRR290 pKa = 11.84DD291 pKa = 3.63PRR293 pKa = 11.84LEE295 pKa = 3.98LWADD299 pKa = 3.46LTDD302 pKa = 4.15RR303 pKa = 11.84SAASKK308 pKa = 10.82RR309 pKa = 11.84DD310 pKa = 3.8MIQSDD315 pKa = 4.44VDD317 pKa = 3.37WLEE320 pKa = 4.03ANCIFVEE327 pKa = 4.16LQEE330 pKa = 4.74PIFRR334 pKa = 11.84ANTEE338 pKa = 3.84

Molecular weight: 37.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8YT75|A0A1P8YT75_9VIRU Replication associated protein OS=Thrips-associated genomovirus 2 OX=1941234 PE=3 SV=1
MM1 pKa = 7.89PYY3 pKa = 10.18LRR5 pKa = 11.84KK6 pKa = 9.48RR7 pKa = 11.84RR8 pKa = 11.84YY9 pKa = 9.05ARR11 pKa = 11.84PRR13 pKa = 11.84YY14 pKa = 9.17SSYY17 pKa = 9.43RR18 pKa = 11.84AKK20 pKa = 10.52RR21 pKa = 11.84RR22 pKa = 11.84TPSSRR27 pKa = 11.84APYY30 pKa = 9.19RR31 pKa = 11.84YY32 pKa = 9.34SRR34 pKa = 11.84KK35 pKa = 8.56RR36 pKa = 11.84TISRR40 pKa = 11.84PRR42 pKa = 11.84YY43 pKa = 8.07AMSRR47 pKa = 11.84KK48 pKa = 9.16RR49 pKa = 11.84LLNITSRR56 pKa = 11.84KK57 pKa = 9.19KK58 pKa = 10.18QDD60 pKa = 3.04TMLVNTNTVAGTPFGGTTFSNTAALLQGGNTYY92 pKa = 10.35IFPWVATARR101 pKa = 11.84DD102 pKa = 3.94NTIGTASGNVFNSADD117 pKa = 3.76RR118 pKa = 11.84TATTCYY124 pKa = 9.66MRR126 pKa = 11.84GLKK129 pKa = 9.81EE130 pKa = 4.23RR131 pKa = 11.84IQIQTNTGMPWQWRR145 pKa = 11.84RR146 pKa = 11.84ICFTMKK152 pKa = 10.67GDD154 pKa = 6.5DD155 pKa = 3.21INAWNEE161 pKa = 3.91SAYY164 pKa = 9.95KK165 pKa = 10.41LQLEE169 pKa = 4.8TSNGWARR176 pKa = 11.84VVSNTGVASNLYY188 pKa = 9.9NNLVPLLFKK197 pKa = 10.7GAQNVDD203 pKa = 2.78WNNPFNAKK211 pKa = 8.72TDD213 pKa = 3.57NTRR216 pKa = 11.84LSIKK220 pKa = 9.94YY221 pKa = 10.31DD222 pKa = 2.75KK223 pKa = 7.68TTPIRR228 pKa = 11.84CGNSNGMMKK237 pKa = 10.28EE238 pKa = 4.27FIRR241 pKa = 11.84WHH243 pKa = 6.23PMNKK247 pKa = 9.58NLVYY251 pKa = 10.83DD252 pKa = 4.27DD253 pKa = 5.28DD254 pKa = 4.83EE255 pKa = 6.02NGEE258 pKa = 4.37KK259 pKa = 9.73KK260 pKa = 10.59DD261 pKa = 3.57EE262 pKa = 4.94AIYY265 pKa = 10.98SVDD268 pKa = 3.49GKK270 pKa = 10.89RR271 pKa = 11.84GMGDD275 pKa = 3.46YY276 pKa = 10.9YY277 pKa = 11.34VIDD280 pKa = 4.8IIAAGSGSTSADD292 pKa = 2.78QLSFNPSATLYY303 pKa = 7.84WHH305 pKa = 6.52EE306 pKa = 4.2RR307 pKa = 3.47

Molecular weight: 35.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

874

229

338

291.3

32.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.696 ± 0.493

1.259 ± 0.171

6.407 ± 0.547

4.691 ± 0.858

3.547 ± 0.25

9.84 ± 1.424

1.945 ± 0.539

3.776 ± 0.488

4.348 ± 0.617

7.895 ± 1.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.517

4.691 ± 1.68

6.064 ± 1.09

2.632 ± 0.015

8.009 ± 0.626

6.178 ± 0.535

5.606 ± 1.445

5.606 ± 0.802

2.632 ± 0.369

4.119 ± 0.58
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski