Georgenia sp. Z446

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Bogoriellaceae; Georgenia; unclassified Georgenia

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3886 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A552WQ72|A0A552WQ72_9MICO DUF3516 domain-containing protein OS=Georgenia sp. Z446 OX=2594180 GN=FJ693_12860 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 4.46FHH4 pKa = 6.24VTLSRR9 pKa = 11.84PVAAVATAAAALLALAACSSGGSAEE34 pKa = 4.14EE35 pKa = 4.5APASAATAGEE45 pKa = 4.18PAPVTIQHH53 pKa = 6.72AFGSTTIPEE62 pKa = 4.02QPEE65 pKa = 4.11NVVTLGWGSTEE76 pKa = 3.58AALALGVVPLGIEE89 pKa = 4.16SQTYY93 pKa = 9.51AADD96 pKa = 3.55EE97 pKa = 4.71HH98 pKa = 6.74GQLPWVAEE106 pKa = 4.03ALTDD110 pKa = 3.95AGAEE114 pKa = 4.06PTMLPATVEE123 pKa = 4.17EE124 pKa = 4.33PAYY127 pKa = 9.7EE128 pKa = 4.12QIGALAPDD136 pKa = 5.85LILAPYY142 pKa = 10.03SGITAEE148 pKa = 4.09QYY150 pKa = 10.81EE151 pKa = 4.96LLSEE155 pKa = 4.5IAPTVAYY162 pKa = 9.57PEE164 pKa = 4.67EE165 pKa = 4.63PWTTPWRR172 pKa = 11.84DD173 pKa = 3.18VITTVGTALGVPDD186 pKa = 4.82KK187 pKa = 11.35ADD189 pKa = 3.62TLVGDD194 pKa = 4.89LDD196 pKa = 3.9AQITEE201 pKa = 4.31AAQAHH206 pKa = 6.9PEE208 pKa = 4.02LAGKK212 pKa = 7.6TVAAVWDD219 pKa = 4.05LSGTFYY225 pKa = 10.98VYY227 pKa = 10.52KK228 pKa = 10.6AQDD231 pKa = 3.29SRR233 pKa = 11.84VDD235 pKa = 3.85FLLDD239 pKa = 3.84LGLVSAPAVDD249 pKa = 4.53EE250 pKa = 4.34LATDD254 pKa = 3.78EE255 pKa = 4.38EE256 pKa = 5.39SFVYY260 pKa = 9.89TLSTEE265 pKa = 3.92EE266 pKa = 4.06TDD268 pKa = 5.25RR269 pKa = 11.84LDD271 pKa = 4.1SDD273 pKa = 3.65ILVNYY278 pKa = 10.11ASTQEE283 pKa = 4.12EE284 pKa = 4.71VDD286 pKa = 3.58TFLGKK291 pKa = 10.23SYY293 pKa = 10.79AQAIPAVQAGAVANITGDD311 pKa = 3.55QLIAAMSPPTALSIDD326 pKa = 3.75WGLDD330 pKa = 3.11TYY332 pKa = 11.94VDD334 pKa = 4.49LLSKK338 pKa = 10.7AAATVKK344 pKa = 10.7

Molecular weight: 35.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A552WKS0|A0A552WKS0_9MICO Branched-chain amino acid ABC transporter OS=Georgenia sp. Z446 OX=2594180 GN=FJ693_18340 PE=4 SV=1
MM1 pKa = 7.53GLLPGPGGRR10 pKa = 11.84RR11 pKa = 11.84AHH13 pKa = 5.64QAAAHH18 pKa = 6.36RR19 pKa = 11.84SGAARR24 pKa = 11.84AALGGVRR31 pKa = 11.84ARR33 pKa = 11.84VQRR36 pKa = 11.84RR37 pKa = 11.84PAGGRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84AAGRR48 pKa = 11.84GTGRR52 pKa = 11.84APRR55 pKa = 11.84PSGGATPGAGGRR67 pKa = 11.84GAA69 pKa = 4.39

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3886

0

3886

1259739

32

2336

324.2

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.37 ± 0.061

0.569 ± 0.009

6.054 ± 0.033

5.577 ± 0.033

2.615 ± 0.021

9.423 ± 0.039

2.202 ± 0.018

3.306 ± 0.03

1.636 ± 0.022

10.317 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.83 ± 0.015

1.678 ± 0.02

5.854 ± 0.03

2.732 ± 0.019

7.797 ± 0.046

4.757 ± 0.024

6.301 ± 0.027

9.656 ± 0.041

1.476 ± 0.016

1.851 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski