Nocardiopsis sp. NRRL B-16309

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Nocardiopsis; unclassified Nocardiopsis

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N0ARF7|A0A0N0ARF7_9ACTN Glycosyl hydrolase family 26 OS=Nocardiopsis sp. NRRL B-16309 OX=1519494 GN=ADL05_04950 PE=3 SV=1
MM1 pKa = 7.91PPRR4 pKa = 11.84TSPRR8 pKa = 11.84HH9 pKa = 4.82LAAPAAPIRR18 pKa = 11.84SAALTASAALAALTLLGACAADD40 pKa = 4.55PDD42 pKa = 4.47PAPDD46 pKa = 3.39QDD48 pKa = 4.21AGTGADD54 pKa = 4.81FPAASLDD61 pKa = 3.53GLRR64 pKa = 11.84VLLANDD70 pKa = 4.48DD71 pKa = 3.86SMQAGEE77 pKa = 4.48EE78 pKa = 4.22DD79 pKa = 4.05GSDD82 pKa = 3.38GLGLYY87 pKa = 7.51EE88 pKa = 5.76LRR90 pKa = 11.84SALCAAGADD99 pKa = 3.54VVVFAPWGYY108 pKa = 9.44QSSMSSAISHH118 pKa = 6.14SGSFGLGAHH127 pKa = 7.16PGPPEE132 pKa = 4.43EE133 pKa = 4.32YY134 pKa = 10.77AGDD137 pKa = 4.07CADD140 pKa = 4.38APSGGAVHH148 pKa = 6.52GVCVADD154 pKa = 5.79GPCEE158 pKa = 4.31DD159 pKa = 5.47DD160 pKa = 4.68SPSATPVDD168 pKa = 4.03SVTFALHH175 pKa = 6.46HH176 pKa = 6.19GLSEE180 pKa = 4.13LVGWDD185 pKa = 3.87GPPDD189 pKa = 3.64LVVSGVNSGPNVASQIANSGTAGAAFAGQGAGVPAIAVSAGLDD232 pKa = 3.37EE233 pKa = 5.58DD234 pKa = 4.1FTVAPRR240 pKa = 11.84TYY242 pKa = 8.86TAAAEE247 pKa = 4.15FSTDD251 pKa = 2.94LVARR255 pKa = 11.84LVGSDD260 pKa = 4.68LLTSDD265 pKa = 3.51YY266 pKa = 9.89MINVNHH272 pKa = 6.51PHH274 pKa = 6.76APDD277 pKa = 4.04GAPGTDD283 pKa = 3.49VRR285 pKa = 11.84WTRR288 pKa = 11.84AGTGTVLIPEE298 pKa = 4.59FTGQDD303 pKa = 3.28AYY305 pKa = 10.65EE306 pKa = 4.36LGVRR310 pKa = 11.84VCEE313 pKa = 4.51PDD315 pKa = 3.18TPGCVPEE322 pKa = 4.64TSADD326 pKa = 3.73ADD328 pKa = 3.93STALLAEE335 pKa = 4.54GAVSVSALTADD346 pKa = 3.43RR347 pKa = 11.84TYY349 pKa = 11.78ASGEE353 pKa = 3.97DD354 pKa = 3.53PAEE357 pKa = 4.12VEE359 pKa = 4.18RR360 pKa = 11.84LAEE363 pKa = 3.98LVAALGGG370 pKa = 3.68

Molecular weight: 36.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0ANB3|A0A0N0ANB3_9ACTN NADH-quinone oxidoreductase subunit I OS=Nocardiopsis sp. NRRL B-16309 OX=1519494 GN=nuoI PE=3 SV=1
MM1 pKa = 7.57LGFLGRR7 pKa = 11.84LRR9 pKa = 11.84LLGRR13 pKa = 11.84LGRR16 pKa = 11.84LPLSHH21 pKa = 7.74PGRR24 pKa = 11.84LGRR27 pKa = 11.84LRR29 pKa = 11.84LVGSLGHH36 pKa = 6.97LPLGPLEE43 pKa = 4.13LRR45 pKa = 11.84GFLRR49 pKa = 11.84LLGLDD54 pKa = 3.09PVMRR58 pKa = 11.84RR59 pKa = 11.84PRR61 pKa = 11.84HH62 pKa = 5.41LGSMGMPSRR71 pKa = 11.84LLHH74 pKa = 6.1GRR76 pKa = 11.84LGTLRR81 pKa = 11.84RR82 pKa = 11.84LLLHH86 pKa = 5.61SLGRR90 pKa = 11.84LGHH93 pKa = 6.51LRR95 pKa = 11.84PIRR98 pKa = 11.84LLVWLLLGRR107 pKa = 11.84LGGLGGAGPVGLPRR121 pKa = 11.84LLDD124 pKa = 3.87LLRR127 pKa = 11.84LRR129 pKa = 11.84ALGLGSVMRR138 pKa = 11.84RR139 pKa = 11.84LGGG142 pKa = 3.31

Molecular weight: 15.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5253

0

5253

1660638

37

4719

316.1

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.173 ± 0.046

0.726 ± 0.009

6.506 ± 0.033

6.391 ± 0.034

2.76 ± 0.019

9.464 ± 0.033

2.367 ± 0.015

3.106 ± 0.023

1.469 ± 0.021

10.215 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.88 ± 0.014

1.662 ± 0.018

6.058 ± 0.038

2.483 ± 0.019

8.29 ± 0.038

5.13 ± 0.026

5.902 ± 0.028

8.927 ± 0.041

1.523 ± 0.013

1.969 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski