Hyphomicrobium sp. xq

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Hyphomicrobium; unclassified Hyphomicrobium

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3664 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I3KGD7|A0A6I3KGD7_9RHIZ M48 family metalloprotease OS=Hyphomicrobium sp. xq OX=2665159 GN=GIW81_06545 PE=4 SV=1
MM1 pKa = 7.88WLTALAVVDD10 pKa = 4.3VGPAHH15 pKa = 6.63AQAITPPSPPEE26 pKa = 4.1CVSVTGIGVEE36 pKa = 4.8IITCTGNLSPGVNLANGPGPYY57 pKa = 9.83DD58 pKa = 3.25ILNVHH63 pKa = 6.44SLTANITPASGTAGILFTSDD83 pKa = 2.73GSVEE87 pKa = 4.35LNVDD91 pKa = 3.89TGPFGIFTTAADD103 pKa = 4.18GVFAAGDD110 pKa = 3.89AGPVTINFFGAITTSGNGAVGIRR133 pKa = 11.84AGGLDD138 pKa = 3.84DD139 pKa = 4.97LVSIVASGTIVTDD152 pKa = 3.75GLSASGIAASTDD164 pKa = 3.05NGPIDD169 pKa = 3.42IRR171 pKa = 11.84SSVSIATQGQSAHH184 pKa = 6.69GIDD187 pKa = 3.76VSSVGGAINILSMGNIATGGTDD209 pKa = 3.95AIGIYY214 pKa = 8.9ATSTTGQITITSTSDD229 pKa = 2.41ITTSGGDD236 pKa = 3.33SVGIFAEE243 pKa = 4.41TSGVAMVEE251 pKa = 4.03SSGNFLTRR259 pKa = 11.84GDD261 pKa = 3.78GAAGIAAAGQTGTAVISTGSIATEE285 pKa = 4.33GSDD288 pKa = 4.2APGITAISDD297 pKa = 3.85AEE299 pKa = 4.33VGVAMSGSIATARR312 pKa = 11.84DD313 pKa = 3.25NSRR316 pKa = 11.84GIWAQSLVNGMVAVGAGGTITTAGDD341 pKa = 3.54DD342 pKa = 4.47SDD344 pKa = 6.08GIWADD349 pKa = 3.46SSSGAVVVISSANITTTGQQSDD371 pKa = 4.77GISAISGAALATVVNFGVVSANGVGSAGIFAEE403 pKa = 4.57GQEE406 pKa = 4.54TQVEE410 pKa = 4.63NYY412 pKa = 7.93GTVAGGPCCGGVMMDD427 pKa = 4.04AVDD430 pKa = 4.4LAVLMNYY437 pKa = 7.76GTIIGQTSGEE447 pKa = 4.32AIFGSGTDD455 pKa = 3.37VRR457 pKa = 11.84IEE459 pKa = 3.93NFGTVTGNVILFGSNSSYY477 pKa = 10.9FFNQAGALFNSGEE490 pKa = 4.09QVMSGFVVNDD500 pKa = 3.51GTIAPGGRR508 pKa = 11.84GSVLTTLMSDD518 pKa = 5.11FYY520 pKa = 11.35SQGAGGTYY528 pKa = 10.47AVDD531 pKa = 5.16LDD533 pKa = 4.21PQASNPSDD541 pKa = 3.58RR542 pKa = 11.84NDD544 pKa = 3.66YY545 pKa = 10.53IVASNNAEE553 pKa = 3.91VAGVVEE559 pKa = 4.52VSVLSLPVAASEE571 pKa = 4.31TFIILTGMSSLTDD584 pKa = 3.29NGLSLIASPALHH596 pKa = 6.08ATLFTDD602 pKa = 3.97ATNVTLGISVDD613 pKa = 4.19FTVDD617 pKa = 3.17SLNPNQRR624 pKa = 11.84HH625 pKa = 5.38IAADD629 pKa = 4.02LNQVFLAGGGGVTPVLLGLLNTGNFDD655 pKa = 3.75AYY657 pKa = 10.15KK658 pKa = 10.58NALNQLSPEE667 pKa = 4.09IYY669 pKa = 10.52SNAEE673 pKa = 3.12IAALYY678 pKa = 10.62SSLAFSNSLLSCKK691 pKa = 10.5VNGTGTGTAAIIRR704 pKa = 11.84EE705 pKa = 4.68GQCLWAGASANFVDD719 pKa = 4.82TGTTSDD725 pKa = 4.03QIGFTQTTGLFNAGAQVALDD745 pKa = 3.85DD746 pKa = 3.66VWRR749 pKa = 11.84VGFGAGYY756 pKa = 10.01QSSSLQTATNAQSEE770 pKa = 4.73GSLAQAGLSVKK781 pKa = 10.23YY782 pKa = 10.83NPGPLLLAGAVSGGGAWYY800 pKa = 7.87DD801 pKa = 3.63TTRR804 pKa = 11.84TMAFGGFTGVAEE816 pKa = 4.56GDD818 pKa = 3.3QDD820 pKa = 3.78VGIVSGSLRR829 pKa = 11.84AAYY832 pKa = 10.32VLGSPHH838 pKa = 7.45LYY840 pKa = 10.07YY841 pKa = 10.72KK842 pKa = 10.61PILDD846 pKa = 4.5LGLTHH851 pKa = 7.27LEE853 pKa = 3.9LGGFTEE859 pKa = 4.57SGGGGAALTVAGGGQTVFSLAPTLEE884 pKa = 4.4VGSEE888 pKa = 3.26WWMSNGTLIRR898 pKa = 11.84PMIRR902 pKa = 11.84GGAIWYY908 pKa = 9.4DD909 pKa = 3.38GADD912 pKa = 3.68FALTAAFAGAPLGVSPFTINTNIDD936 pKa = 3.26EE937 pKa = 4.35VMGLVGAGVEE947 pKa = 4.46VINGGDD953 pKa = 3.07AALRR957 pKa = 11.84FSYY960 pKa = 10.38DD961 pKa = 3.09GQLGEE966 pKa = 4.3TTQIHH971 pKa = 4.78TVGVKK976 pKa = 10.14GSARR980 pKa = 11.84FF981 pKa = 3.39

Molecular weight: 97.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I3KQD6|A0A6I3KQD6_9RHIZ Delta-aminolevulinic acid dehydratase OS=Hyphomicrobium sp. xq OX=2665159 GN=hemB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.26RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.73GFRR19 pKa = 11.84KK20 pKa = 10.06RR21 pKa = 11.84MQTTGGVRR29 pKa = 11.84VLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3664

0

3664

1132951

29

4183

309.2

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.811 ± 0.058

0.871 ± 0.018

5.633 ± 0.032

5.671 ± 0.042

3.531 ± 0.022

8.57 ± 0.072

1.963 ± 0.022

4.935 ± 0.029

3.774 ± 0.04

9.826 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.237 ± 0.021

2.652 ± 0.034

5.313 ± 0.038

3.134 ± 0.024

7.031 ± 0.051

5.467 ± 0.038

5.401 ± 0.04

7.563 ± 0.039

1.309 ± 0.018

2.306 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski