Amino acid dipepetide frequency for Bombilactobacillus mellis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.843AlaAla: 7.843 ± 0.183
0.486AlaCys: 0.486 ± 0.033
4.355AlaAsp: 4.355 ± 0.094
3.458AlaGlu: 3.458 ± 0.094
2.845AlaPhe: 2.845 ± 0.083
5.493AlaGly: 5.493 ± 0.12
1.674AlaHis: 1.674 ± 0.06
5.712AlaIle: 5.712 ± 0.123
4.081AlaLys: 4.081 ± 0.104
7.862AlaLeu: 7.862 ± 0.131
1.574AlaMet: 1.574 ± 0.059
3.646AlaAsn: 3.646 ± 0.1
2.567AlaPro: 2.567 ± 0.081
5.896AlaGln: 5.896 ± 0.145
2.608AlaArg: 2.608 ± 0.08
3.875AlaSer: 3.875 ± 0.091
4.412AlaThr: 4.412 ± 0.106
5.375AlaVal: 5.375 ± 0.111
0.613AlaTrp: 0.613 ± 0.035
2.434AlaTyr: 2.434 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
0.413CysAla: 0.413 ± 0.029
0.076CysCys: 0.076 ± 0.011
0.304CysAsp: 0.304 ± 0.025
0.223CysGlu: 0.223 ± 0.02
0.309CysPhe: 0.309 ± 0.025
0.57CysGly: 0.57 ± 0.034
0.198CysHis: 0.198 ± 0.022
0.376CysIle: 0.376 ± 0.028
0.211CysLys: 0.211 ± 0.019
0.84CysLeu: 0.84 ± 0.043
0.116CysMet: 0.116 ± 0.016
0.268CysAsn: 0.268 ± 0.022
0.282CysPro: 0.282 ± 0.027
0.427CysGln: 0.427 ± 0.032
0.268CysArg: 0.268 ± 0.023
0.396CysSer: 0.396 ± 0.026
0.294CysThr: 0.294 ± 0.027
0.313CysVal: 0.313 ± 0.024
0.076CysTrp: 0.076 ± 0.011
0.255CysTyr: 0.255 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.098AspAla: 3.098 ± 0.08
0.343AspCys: 0.343 ± 0.028
2.604AspAsp: 2.604 ± 0.091
2.74AspGlu: 2.74 ± 0.081
2.787AspPhe: 2.787 ± 0.065
2.743AspGly: 2.743 ± 0.083
1.271AspHis: 1.271 ± 0.05
3.809AspIle: 3.809 ± 0.093
3.047AspLys: 3.047 ± 0.104
5.8AspLeu: 5.8 ± 0.106
1.116AspMet: 1.116 ± 0.048
2.935AspAsn: 2.935 ± 0.096
2.324AspPro: 2.324 ± 0.069
3.108AspGln: 3.108 ± 0.09
1.786AspArg: 1.786 ± 0.054
2.973AspSer: 2.973 ± 0.091
2.62AspThr: 2.62 ± 0.079
3.217AspVal: 3.217 ± 0.093
0.711AspTrp: 0.711 ± 0.04
2.685AspTyr: 2.685 ± 0.081
0.0AspXaa: 0.0 ± 0.0
Glu
3.186GluAla: 3.186 ± 0.081
0.208GluCys: 0.208 ± 0.022
2.066GluAsp: 2.066 ± 0.069
2.471GluGlu: 2.471 ± 0.09
1.804GluPhe: 1.804 ± 0.063
2.021GluGly: 2.021 ± 0.071
1.14GluHis: 1.14 ± 0.05
4.059GluIle: 4.059 ± 0.108
3.505GluLys: 3.505 ± 0.103
4.733GluLeu: 4.733 ± 0.122
1.355GluMet: 1.355 ± 0.055
2.687GluAsn: 2.687 ± 0.072
1.498GluPro: 1.498 ± 0.06
3.307GluGln: 3.307 ± 0.081
1.884GluArg: 1.884 ± 0.079
2.152GluSer: 2.152 ± 0.07
2.358GluThr: 2.358 ± 0.067
3.141GluVal: 3.141 ± 0.08
0.401GluTrp: 0.401 ± 0.029
1.704GluTyr: 1.704 ± 0.061
0.0GluXaa: 0.0 ± 0.0
Phe
3.186PheAla: 3.186 ± 0.075
0.304PheCys: 0.304 ± 0.028
2.397PheAsp: 2.397 ± 0.077
1.71PheGlu: 1.71 ± 0.059
2.005PhePhe: 2.005 ± 0.075
2.775PheGly: 2.775 ± 0.08
0.934PheHis: 0.934 ± 0.045
3.307PheIle: 3.307 ± 0.097
2.33PheLys: 2.33 ± 0.072
4.604PheLeu: 4.604 ± 0.12
0.922PheMet: 0.922 ± 0.044
2.497PheAsn: 2.497 ± 0.071
1.518PhePro: 1.518 ± 0.058
2.16PheGln: 2.16 ± 0.063
1.249PheArg: 1.249 ± 0.056
3.092PheSer: 3.092 ± 0.085
2.456PheThr: 2.456 ± 0.079
2.751PheVal: 2.751 ± 0.084
0.601PheTrp: 0.601 ± 0.038
1.729PheTyr: 1.729 ± 0.061
0.0PheXaa: 0.0 ± 0.0
Gly
4.334GlyAla: 4.334 ± 0.115
0.529GlyCys: 0.529 ± 0.034
3.07GlyAsp: 3.07 ± 0.085
2.663GlyGlu: 2.663 ± 0.081
2.9GlyPhe: 2.9 ± 0.085
3.983GlyGly: 3.983 ± 0.115
1.475GlyHis: 1.475 ± 0.051
5.297GlyIle: 5.297 ± 0.104
3.973GlyLys: 3.973 ± 0.092
6.403GlyLeu: 6.403 ± 0.136
1.596GlyMet: 1.596 ± 0.054
2.732GlyAsn: 2.732 ± 0.088
1.729GlyPro: 1.729 ± 0.056
3.27GlyGln: 3.27 ± 0.088
2.322GlyArg: 2.322 ± 0.071
3.846GlySer: 3.846 ± 0.097
3.795GlyThr: 3.795 ± 0.101
4.095GlyVal: 4.095 ± 0.097
0.815GlyTrp: 0.815 ± 0.049
2.632GlyTyr: 2.632 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
1.232HisAla: 1.232 ± 0.048
0.178HisCys: 0.178 ± 0.019
1.177HisAsp: 1.177 ± 0.055
0.963HisGlu: 0.963 ± 0.042
1.179HisPhe: 1.179 ± 0.052
1.439HisGly: 1.439 ± 0.055
0.742HisHis: 0.742 ± 0.041
1.388HisIle: 1.388 ± 0.051
1.095HisLys: 1.095 ± 0.048
2.847HisLeu: 2.847 ± 0.08
0.425HisMet: 0.425 ± 0.028
1.259HisAsn: 1.259 ± 0.058
1.151HisPro: 1.151 ± 0.045
1.537HisGln: 1.537 ± 0.053
0.875HisArg: 0.875 ± 0.041
1.296HisSer: 1.296 ± 0.048
1.057HisThr: 1.057 ± 0.043
1.263HisVal: 1.263 ± 0.047
0.319HisTrp: 0.319 ± 0.026
1.155HisTyr: 1.155 ± 0.049
0.0HisXaa: 0.0 ± 0.0
Ile
6.347IleAla: 6.347 ± 0.12
0.672IleCys: 0.672 ± 0.046
4.157IleAsp: 4.157 ± 0.111
3.372IleGlu: 3.372 ± 0.097
3.578IlePhe: 3.578 ± 0.112
5.054IleGly: 5.054 ± 0.136
1.326IleHis: 1.326 ± 0.055
6.969IleIle: 6.969 ± 0.164
5.201IleLys: 5.201 ± 0.113
7.645IleLeu: 7.645 ± 0.166
1.909IleMet: 1.909 ± 0.06
4.729IleAsn: 4.729 ± 0.107
2.957IlePro: 2.957 ± 0.072
3.37IleGln: 3.37 ± 0.085
2.536IleArg: 2.536 ± 0.079
5.015IleSer: 5.015 ± 0.12
4.909IleThr: 4.909 ± 0.092
5.119IleVal: 5.119 ± 0.109
0.717IleTrp: 0.717 ± 0.04
2.659IleTyr: 2.659 ± 0.079
0.0IleXaa: 0.0 ± 0.0
Lys
4.234LysAla: 4.234 ± 0.096
0.208LysCys: 0.208 ± 0.02
3.202LysAsp: 3.202 ± 0.096
3.147LysGlu: 3.147 ± 0.089
2.301LysPhe: 2.301 ± 0.068
2.853LysGly: 2.853 ± 0.08
1.195LysHis: 1.195 ± 0.049
5.591LysIle: 5.591 ± 0.115
5.193LysLys: 5.193 ± 0.119
6.18LysLeu: 6.18 ± 0.109
1.966LysMet: 1.966 ± 0.061
4.157LysAsn: 4.157 ± 0.108
2.125LysPro: 2.125 ± 0.066
3.787LysGln: 3.787 ± 0.091
2.71LysArg: 2.71 ± 0.081
3.341LysSer: 3.341 ± 0.084
3.797LysThr: 3.797 ± 0.089
3.952LysVal: 3.952 ± 0.096
0.564LysTrp: 0.564 ± 0.036
2.716LysTyr: 2.716 ± 0.068
0.0LysXaa: 0.0 ± 0.0
Leu
8.832LeuAla: 8.832 ± 0.154
0.691LeuCys: 0.691 ± 0.04
5.399LeuAsp: 5.399 ± 0.129
4.704LeuGlu: 4.704 ± 0.114
4.093LeuPhe: 4.093 ± 0.102
6.607LeuGly: 6.607 ± 0.128
2.101LeuHis: 2.101 ± 0.062
8.228LeuIle: 8.228 ± 0.166
6.766LeuLys: 6.766 ± 0.137
10.545LeuLeu: 10.545 ± 0.246
2.491LeuMet: 2.491 ± 0.075
5.524LeuAsn: 5.524 ± 0.113
4.328LeuPro: 4.328 ± 0.099
6.613LeuGln: 6.613 ± 0.148
3.728LeuArg: 3.728 ± 0.097
6.486LeuSer: 6.486 ± 0.11
6.503LeuThr: 6.503 ± 0.115
6.781LeuVal: 6.781 ± 0.148
0.889LeuTrp: 0.889 ± 0.047
3.153LeuTyr: 3.153 ± 0.08
0.0LeuXaa: 0.0 ± 0.0
Met
1.982MetAla: 1.982 ± 0.07
0.108MetCys: 0.108 ± 0.013
1.198MetAsp: 1.198 ± 0.054
0.993MetGlu: 0.993 ± 0.046
0.981MetPhe: 0.981 ± 0.062
1.551MetGly: 1.551 ± 0.052
0.409MetHis: 0.409 ± 0.03
1.874MetIle: 1.874 ± 0.063
1.757MetLys: 1.757 ± 0.06
2.072MetLeu: 2.072 ± 0.061
0.625MetMet: 0.625 ± 0.039
1.279MetAsn: 1.279 ± 0.046
0.938MetPro: 0.938 ± 0.044
1.386MetGln: 1.386 ± 0.057
0.862MetArg: 0.862 ± 0.037
1.537MetSer: 1.537 ± 0.062
1.639MetThr: 1.639 ± 0.063
1.414MetVal: 1.414 ± 0.054
0.178MetTrp: 0.178 ± 0.017
0.595MetTyr: 0.595 ± 0.038
0.0MetXaa: 0.0 ± 0.0
Asn
3.302AsnAla: 3.302 ± 0.083
0.364AsnCys: 0.364 ± 0.025
2.845AsnAsp: 2.845 ± 0.085
2.393AsnGlu: 2.393 ± 0.077
2.71AsnPhe: 2.71 ± 0.081
3.206AsnGly: 3.206 ± 0.098
1.379AsnHis: 1.379 ± 0.062
3.92AsnIle: 3.92 ± 0.104
3.503AsnLys: 3.503 ± 0.094
5.755AsnLeu: 5.755 ± 0.112
1.339AsnMet: 1.339 ± 0.05
3.946AsnAsn: 3.946 ± 0.142
2.43AsnPro: 2.43 ± 0.071
3.347AsnGln: 3.347 ± 0.096
1.778AsnArg: 1.778 ± 0.068
3.621AsnSer: 3.621 ± 0.118
2.655AsnThr: 2.655 ± 0.083
3.022AsnVal: 3.022 ± 0.086
0.74AsnTrp: 0.74 ± 0.04
2.649AsnTyr: 2.649 ± 0.082
0.0AsnXaa: 0.0 ± 0.0
Pro
3.025ProAla: 3.025 ± 0.084
0.149ProCys: 0.149 ± 0.018
2.158ProAsp: 2.158 ± 0.064
2.209ProGlu: 2.209 ± 0.077
1.508ProPhe: 1.508 ± 0.053
2.127ProGly: 2.127 ± 0.075
0.948ProHis: 0.948 ± 0.043
2.614ProIle: 2.614 ± 0.074
2.076ProLys: 2.076 ± 0.066
4.003ProLeu: 4.003 ± 0.096
0.644ProMet: 0.644 ± 0.032
2.054ProAsn: 2.054 ± 0.073
0.826ProPro: 0.826 ± 0.046
3.239ProGln: 3.239 ± 0.08
1.226ProArg: 1.226 ± 0.052
1.982ProSer: 1.982 ± 0.063
2.399ProThr: 2.399 ± 0.069
2.657ProVal: 2.657 ± 0.075
0.431ProTrp: 0.431 ± 0.035
1.435ProTyr: 1.435 ± 0.06
0.0ProXaa: 0.0 ± 0.0
Gln
5.653GlnAla: 5.653 ± 0.132
0.204GlnCys: 0.204 ± 0.021
2.757GlnAsp: 2.757 ± 0.088
3.352GlnGlu: 3.352 ± 0.091
2.007GlnPhe: 2.007 ± 0.063
3.096GlnGly: 3.096 ± 0.091
1.512GlnHis: 1.512 ± 0.058
4.909GlnIle: 4.909 ± 0.128
5.236GlnLys: 5.236 ± 0.121
6.903GlnLeu: 6.903 ± 0.168
1.484GlnMet: 1.484 ± 0.052
3.243GlnAsn: 3.243 ± 0.096
2.463GlnPro: 2.463 ± 0.08
5.975GlnGln: 5.975 ± 0.227
2.794GlnArg: 2.794 ± 0.09
2.92GlnSer: 2.92 ± 0.071
3.646GlnThr: 3.646 ± 0.094
4.42GlnVal: 4.42 ± 0.112
0.558GlnTrp: 0.558 ± 0.038
1.913GlnTyr: 1.913 ± 0.058
0.0GlnXaa: 0.0 ± 0.0
Arg
2.444ArgAla: 2.444 ± 0.074
0.188ArgCys: 0.188 ± 0.019
1.727ArgAsp: 1.727 ± 0.071
1.643ArgGlu: 1.643 ± 0.071
1.531ArgPhe: 1.531 ± 0.051
2.005ArgGly: 2.005 ± 0.064
1.001ArgHis: 1.001 ± 0.048
2.704ArgIle: 2.704 ± 0.073
2.338ArgLys: 2.338 ± 0.074
3.92ArgLeu: 3.92 ± 0.1
0.848ArgMet: 0.848 ± 0.043
1.829ArgAsn: 1.829 ± 0.065
1.496ArgPro: 1.496 ± 0.057
3.02ArgGln: 3.02 ± 0.094
1.837ArgArg: 1.837 ± 0.062
2.095ArgSer: 2.095 ± 0.059
1.794ArgThr: 1.794 ± 0.058
2.179ArgVal: 2.179 ± 0.069
0.411ArgTrp: 0.411 ± 0.027
1.512ArgTyr: 1.512 ± 0.052
0.0ArgXaa: 0.0 ± 0.0
Ser
4.271SerAla: 4.271 ± 0.089
0.354SerCys: 0.354 ± 0.028
3.104SerAsp: 3.104 ± 0.083
2.648SerGlu: 2.648 ± 0.068
2.649SerPhe: 2.649 ± 0.079
4.33SerGly: 4.33 ± 0.1
1.314SerHis: 1.314 ± 0.054
4.042SerIle: 4.042 ± 0.105
3.392SerLys: 3.392 ± 0.099
6.311SerLeu: 6.311 ± 0.121
1.367SerMet: 1.367 ± 0.056
3.206SerAsn: 3.206 ± 0.104
2.062SerPro: 2.062 ± 0.07
3.848SerGln: 3.848 ± 0.085
2.148SerArg: 2.148 ± 0.059
3.909SerSer: 3.909 ± 0.097
3.127SerThr: 3.127 ± 0.075
3.652SerVal: 3.652 ± 0.101
0.834SerTrp: 0.834 ± 0.047
2.297SerTyr: 2.297 ± 0.07
0.0SerXaa: 0.0 ± 0.0
Thr
5.05ThrAla: 5.05 ± 0.107
0.307ThrCys: 0.307 ± 0.028
3.159ThrAsp: 3.159 ± 0.098
2.275ThrGlu: 2.275 ± 0.063
2.338ThrPhe: 2.338 ± 0.082
4.077ThrGly: 4.077 ± 0.097
1.196ThrHis: 1.196 ± 0.053
4.97ThrIle: 4.97 ± 0.098
3.568ThrLys: 3.568 ± 0.085
5.722ThrLeu: 5.722 ± 0.123
1.155ThrMet: 1.155 ± 0.045
3.174ThrAsn: 3.174 ± 0.106
2.624ThrPro: 2.624 ± 0.076
2.882ThrGln: 2.882 ± 0.083
1.619ThrArg: 1.619 ± 0.059
3.523ThrSer: 3.523 ± 0.101
3.885ThrThr: 3.885 ± 0.107
3.973ThrVal: 3.973 ± 0.097
0.648ThrTrp: 0.648 ± 0.041
1.852ThrTyr: 1.852 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
5.489ValAla: 5.489 ± 0.129
0.46ValCys: 0.46 ± 0.032
3.521ValAsp: 3.521 ± 0.093
2.734ValGlu: 2.734 ± 0.078
2.567ValPhe: 2.567 ± 0.079
4.371ValGly: 4.371 ± 0.111
1.343ValHis: 1.343 ± 0.051
5.503ValIle: 5.503 ± 0.113
3.911ValLys: 3.911 ± 0.103
6.258ValLeu: 6.258 ± 0.131
1.561ValMet: 1.561 ± 0.057
3.354ValAsn: 3.354 ± 0.097
2.395ValPro: 2.395 ± 0.072
3.509ValGln: 3.509 ± 0.091
2.156ValArg: 2.156 ± 0.076
3.924ValSer: 3.924 ± 0.107
4.255ValThr: 4.255 ± 0.1
4.478ValVal: 4.478 ± 0.103
0.67ValTrp: 0.67 ± 0.038
2.025ValTyr: 2.025 ± 0.062
0.0ValXaa: 0.0 ± 0.0
Trp
0.611TrpAla: 0.611 ± 0.036
0.07TrpCys: 0.07 ± 0.011
0.46TrpAsp: 0.46 ± 0.031
0.421TrpGlu: 0.421 ± 0.027
0.513TrpPhe: 0.513 ± 0.035
0.775TrpGly: 0.775 ± 0.041
0.333TrpHis: 0.333 ± 0.029
0.711TrpIle: 0.711 ± 0.046
0.474TrpLys: 0.474 ± 0.038
1.406TrpLeu: 1.406 ± 0.056
0.237TrpMet: 0.237 ± 0.024
0.515TrpAsn: 0.515 ± 0.035
0.421TrpPro: 0.421 ± 0.031
1.024TrpGln: 1.024 ± 0.061
0.505TrpArg: 0.505 ± 0.032
0.721TrpSer: 0.721 ± 0.04
0.558TrpThr: 0.558 ± 0.034
0.587TrpVal: 0.587 ± 0.036
0.168TrpTrp: 0.168 ± 0.019
0.327TrpTyr: 0.327 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.281TyrAla: 2.281 ± 0.07
0.317TyrCys: 0.317 ± 0.023
2.056TyrAsp: 2.056 ± 0.072
1.561TyrGlu: 1.561 ± 0.06
1.921TyrPhe: 1.921 ± 0.074
2.383TyrGly: 2.383 ± 0.077
1.091TyrHis: 1.091 ± 0.043
2.258TyrIle: 2.258 ± 0.069
1.561TyrLys: 1.561 ± 0.07
4.651TyrLeu: 4.651 ± 0.118
0.644TyrMet: 0.644 ± 0.039
1.87TyrAsn: 1.87 ± 0.066
1.574TyrPro: 1.574 ± 0.053
3.341TyrGln: 3.341 ± 0.095
1.629TyrArg: 1.629 ± 0.064
2.162TyrSer: 2.162 ± 0.066
1.841TyrThr: 1.841 ± 0.069
2.093TyrVal: 2.093 ± 0.062
0.493TyrTrp: 0.493 ± 0.03
1.619TyrTyr: 1.619 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1572 proteins (510670 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski