Brevundimonas subvibrioides (strain ATCC 15264 / DSM 4735 / LMG 14903 / NBRC 16000 / CB 81) (Caulobacter subvibrioides)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Brevundimonas; Brevundimonas subvibrioides

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3326 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9QFS0|D9QFS0_BRESC Lytic transglycosylase catalytic OS=Brevundimonas subvibrioides (strain ATCC 15264 / DSM 4735 / LMG 14903 / NBRC 16000 / CB 81) OX=633149 GN=Bresu_1322 PE=3 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84NVLSIVAVIAALSAAGAAAAQDD24 pKa = 3.72PSANAGGASRR34 pKa = 11.84KK35 pKa = 9.27NAGFTPDD42 pKa = 4.71PITVNVYY49 pKa = 10.34SGGGIDD55 pKa = 5.59ASDD58 pKa = 3.66TLGGSCVGMIAGAPDD73 pKa = 3.32YY74 pKa = 10.73EE75 pKa = 4.41FTYY78 pKa = 10.12TAGSFPLSFQVRR90 pKa = 11.84SQGDD94 pKa = 3.22TSLIINGPDD103 pKa = 3.25GRR105 pKa = 11.84WYY107 pKa = 10.91CDD109 pKa = 3.67DD110 pKa = 4.48DD111 pKa = 4.48TNGLNPEE118 pKa = 4.41LTWGRR123 pKa = 11.84PGSGSYY129 pKa = 10.06DD130 pKa = 2.68IWVGAVGDD138 pKa = 3.93AASATLYY145 pKa = 9.13ITEE148 pKa = 4.39TPP150 pKa = 3.5

Molecular weight: 15.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9QLV7|D9QLV7_BRESC Uncharacterized protein OS=Brevundimonas subvibrioides (strain ATCC 15264 / DSM 4735 / LMG 14903 / NBRC 16000 / CB 81) OX=633149 GN=Bresu_0726 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.2NGQKK29 pKa = 9.53IVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.14GRR39 pKa = 11.84KK40 pKa = 9.04KK41 pKa = 9.67LTAA44 pKa = 4.2

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3326

0

3326

1043574

30

2668

313.8

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.722 ± 0.057

0.7 ± 0.013

6.151 ± 0.035

5.353 ± 0.042

3.491 ± 0.027

9.23 ± 0.046

1.74 ± 0.02

4.492 ± 0.028

2.453 ± 0.03

9.759 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.021

2.347 ± 0.03

5.559 ± 0.034

3.027 ± 0.022

7.448 ± 0.044

5.228 ± 0.031

5.775 ± 0.035

7.757 ± 0.034

1.464 ± 0.018

2.037 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski