Streptococcus phage Javan115

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZZ28|A0A4D5ZZ28_9CAUD Minor capsid protein OS=Streptococcus phage Javan115 OX=2547984 GN=Javan115_0038 PE=4 SV=1
MM1 pKa = 7.47ATTNDD6 pKa = 3.3VILFAKK12 pKa = 10.33NLADD16 pKa = 3.54NGIGVDD22 pKa = 4.57QDD24 pKa = 4.28GAWGTQCVDD33 pKa = 3.7LPNAISSQLFGKK45 pKa = 9.98ALWGNAIDD53 pKa = 4.91LLNSAASLGYY63 pKa = 9.3EE64 pKa = 3.96VEE66 pKa = 4.24YY67 pKa = 11.55NEE69 pKa = 5.12AGNMDD74 pKa = 4.34SKK76 pKa = 10.91PRR78 pKa = 11.84ASAVFVMEE86 pKa = 3.7TVYY89 pKa = 10.34IYY91 pKa = 9.45GHH93 pKa = 7.27PYY95 pKa = 8.7GHH97 pKa = 6.63TGVVIEE103 pKa = 5.28DD104 pKa = 3.36SDD106 pKa = 4.79GYY108 pKa = 8.51TMKK111 pKa = 10.45TIEE114 pKa = 4.48QNIDD118 pKa = 2.88GNADD122 pKa = 3.2SLYY125 pKa = 9.68IGGPARR131 pKa = 11.84YY132 pKa = 7.47NTRR135 pKa = 11.84NFDD138 pKa = 3.81GVVGWFYY145 pKa = 11.19FPTDD149 pKa = 3.55DD150 pKa = 4.64TSYY153 pKa = 10.51TPATASEE160 pKa = 4.27PFSGEE165 pKa = 3.96VEE167 pKa = 3.77IHH169 pKa = 6.13EE170 pKa = 4.41EE171 pKa = 3.95SGTFTVEE178 pKa = 3.56VSALNVRR185 pKa = 11.84IAAGLNAEE193 pKa = 4.11IVAVYY198 pKa = 8.59TAGQEE203 pKa = 3.74INYY206 pKa = 10.13DD207 pKa = 3.6GWCDD211 pKa = 3.22KK212 pKa = 11.15DD213 pKa = 3.94GYY215 pKa = 10.65IWITYY220 pKa = 9.66IGGSGNRR227 pKa = 11.84RR228 pKa = 11.84YY229 pKa = 10.29VAVGQSEE236 pKa = 3.96KK237 pKa = 10.41GQRR240 pKa = 11.84VTSFGSFKK248 pKa = 11.09

Molecular weight: 26.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A0J2|A0A4D6A0J2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan115 OX=2547984 GN=Javan115_0049 PE=4 SV=1
MM1 pKa = 7.66ADD3 pKa = 3.04VRR5 pKa = 11.84VVVDD9 pKa = 3.6LGGVEE14 pKa = 4.3RR15 pKa = 11.84KK16 pKa = 9.68VSPEE20 pKa = 3.34NLKK23 pKa = 10.57RR24 pKa = 11.84GKK26 pKa = 9.75LAAASQAKK34 pKa = 10.29LIMIPYY40 pKa = 9.24IPMRR44 pKa = 11.84GGDD47 pKa = 3.62LRR49 pKa = 11.84ASGRR53 pKa = 11.84VEE55 pKa = 4.26SNGDD59 pKa = 3.05VSYY62 pKa = 9.23NTVYY66 pKa = 10.97ARR68 pKa = 11.84AHH70 pKa = 6.21FYY72 pKa = 8.24GTNGIVVFRR81 pKa = 11.84RR82 pKa = 11.84YY83 pKa = 5.93TTPGTGKK90 pKa = 10.34RR91 pKa = 11.84WDD93 pKa = 4.0KK94 pKa = 10.31PLKK97 pKa = 11.01ANVEE101 pKa = 4.0QLKK104 pKa = 10.14RR105 pKa = 11.84VAIRR109 pKa = 11.84AMGLRR114 pKa = 3.84

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11467

39

1206

220.5

24.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.163 ± 0.936

0.497 ± 0.075

6.671 ± 0.356

6.575 ± 0.565

3.968 ± 0.198

6.523 ± 0.531

1.456 ± 0.173

6.881 ± 0.266

7.936 ± 0.48

7.979 ± 0.334

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.181

5.468 ± 0.372

2.939 ± 0.226

4.36 ± 0.254

3.855 ± 0.278

6.349 ± 0.453

6.392 ± 0.37

6.584 ± 0.285

1.116 ± 0.146

3.994 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski