Candidatus Mikella endobia

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; Candidatus Mikella

Average proteome isoelectric point is 7.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143WQU5|A0A143WQU5_9ENTR DNA gyrase subunit A OS=Candidatus Mikella endobia OX=1778264 GN=gyrA PE=3 SV=1
MM1 pKa = 7.06STIADD6 pKa = 3.68HH7 pKa = 6.09VKK9 pKa = 10.6EE10 pKa = 4.6IIAEE14 pKa = 3.91QLEE17 pKa = 4.54VKK19 pKa = 10.18KK20 pKa = 10.88EE21 pKa = 3.98KK22 pKa = 10.63VVNSASFVDD31 pKa = 5.12DD32 pKa = 5.07LGADD36 pKa = 3.44SLDD39 pKa = 3.85TIEE42 pKa = 6.4LIMALEE48 pKa = 4.2EE49 pKa = 4.1EE50 pKa = 4.47FDD52 pKa = 4.19IEE54 pKa = 4.28ITDD57 pKa = 3.73EE58 pKa = 4.06EE59 pKa = 4.61AEE61 pKa = 4.53KK62 pKa = 9.67ITTVQEE68 pKa = 4.55AIDD71 pKa = 4.53FIQSNQQ77 pKa = 2.99

Molecular weight: 8.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143WPY8|A0A143WPY8_9ENTR DNA-binding protein OS=Candidatus Mikella endobia OX=1778264 GN=hns PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.68RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.03GFRR19 pKa = 11.84IRR21 pKa = 11.84MANKK25 pKa = 9.4NGRR28 pKa = 11.84HH29 pKa = 4.49ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.53GRR39 pKa = 11.84LCLTVSSKK47 pKa = 11.27

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

273

0

273

88606

38

1405

324.6

36.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.175 ± 0.112

1.32 ± 0.047

4.69 ± 0.085

5.789 ± 0.14

3.921 ± 0.095

6.067 ± 0.126

2.283 ± 0.062

10.217 ± 0.151

7.41 ± 0.139

10.011 ± 0.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.05

6.167 ± 0.096

3.482 ± 0.061

3.861 ± 0.085

5.322 ± 0.103

5.838 ± 0.078

5.151 ± 0.064

5.576 ± 0.118

0.916 ± 0.062

3.43 ± 0.079
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski