Amino acid dipepetide frequency for Shigella phage Ss-VASD

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.037AlaAla: 9.037 ± 0.828
1.123AlaCys: 1.123 ± 0.297
5.508AlaAsp: 5.508 ± 0.566
8.235AlaGlu: 8.235 ± 0.77
3.529AlaPhe: 3.529 ± 0.553
8.396AlaGly: 8.396 ± 1.351
1.444AlaHis: 1.444 ± 0.33
4.278AlaIle: 4.278 ± 0.609
5.348AlaLys: 5.348 ± 0.496
6.738AlaLeu: 6.738 ± 0.422
2.995AlaMet: 2.995 ± 0.454
2.727AlaAsn: 2.727 ± 0.397
3.369AlaPro: 3.369 ± 0.426
5.027AlaGln: 5.027 ± 0.771
5.241AlaArg: 5.241 ± 0.598
5.936AlaSer: 5.936 ± 0.423
5.294AlaThr: 5.294 ± 0.708
6.631AlaVal: 6.631 ± 0.649
1.604AlaTrp: 1.604 ± 0.304
2.567AlaTyr: 2.567 ± 0.312
0.0AlaXaa: 0.0 ± 0.0
Cys
0.909CysAla: 0.909 ± 0.232
0.374CysCys: 0.374 ± 0.135
0.642CysAsp: 0.642 ± 0.223
0.642CysGlu: 0.642 ± 0.174
0.535CysPhe: 0.535 ± 0.175
0.963CysGly: 0.963 ± 0.327
0.374CysHis: 0.374 ± 0.155
0.535CysIle: 0.535 ± 0.195
0.588CysLys: 0.588 ± 0.194
1.176CysLeu: 1.176 ± 0.276
0.214CysMet: 0.214 ± 0.109
0.374CysAsn: 0.374 ± 0.15
0.856CysPro: 0.856 ± 0.238
0.267CysGln: 0.267 ± 0.11
0.909CysArg: 0.909 ± 0.26
0.909CysSer: 0.909 ± 0.251
0.321CysThr: 0.321 ± 0.137
1.07CysVal: 1.07 ± 0.264
0.214CysTrp: 0.214 ± 0.1
0.267CysTyr: 0.267 ± 0.124
0.0CysXaa: 0.0 ± 0.0
Asp
5.722AspAla: 5.722 ± 0.641
0.481AspCys: 0.481 ± 0.15
3.904AspAsp: 3.904 ± 0.399
4.278AspGlu: 4.278 ± 0.462
2.086AspPhe: 2.086 ± 0.342
4.652AspGly: 4.652 ± 0.491
0.802AspHis: 0.802 ± 0.213
3.369AspIle: 3.369 ± 0.406
3.743AspLys: 3.743 ± 0.406
4.118AspLeu: 4.118 ± 0.49
1.925AspMet: 1.925 ± 0.314
2.353AspAsn: 2.353 ± 0.346
2.299AspPro: 2.299 ± 0.407
1.497AspGln: 1.497 ± 0.281
3.85AspArg: 3.85 ± 0.419
2.674AspSer: 2.674 ± 0.316
2.941AspThr: 2.941 ± 0.474
4.278AspVal: 4.278 ± 0.437
0.856AspTrp: 0.856 ± 0.247
1.765AspTyr: 1.765 ± 0.262
0.0AspXaa: 0.0 ± 0.0
Glu
6.417GluAla: 6.417 ± 0.536
1.07GluCys: 1.07 ± 0.286
2.46GluAsp: 2.46 ± 0.258
4.492GluGlu: 4.492 ± 0.515
2.727GluPhe: 2.727 ± 0.496
3.85GluGly: 3.85 ± 0.417
1.658GluHis: 1.658 ± 0.316
3.743GluIle: 3.743 ± 0.434
4.599GluLys: 4.599 ± 0.612
5.882GluLeu: 5.882 ± 0.478
2.086GluMet: 2.086 ± 0.347
2.781GluAsn: 2.781 ± 0.373
1.765GluPro: 1.765 ± 0.397
3.797GluGln: 3.797 ± 0.596
5.294GluArg: 5.294 ± 0.548
3.636GluSer: 3.636 ± 0.455
3.583GluThr: 3.583 ± 0.636
4.599GluVal: 4.599 ± 0.592
0.588GluTrp: 0.588 ± 0.162
2.193GluTyr: 2.193 ± 0.338
0.0GluXaa: 0.0 ± 0.0
Phe
2.781PheAla: 2.781 ± 0.372
0.909PheCys: 0.909 ± 0.248
2.139PheAsp: 2.139 ± 0.368
1.711PheGlu: 1.711 ± 0.379
1.07PhePhe: 1.07 ± 0.235
2.299PheGly: 2.299 ± 0.269
0.588PheHis: 0.588 ± 0.158
1.872PheIle: 1.872 ± 0.309
1.711PheLys: 1.711 ± 0.247
1.925PheLeu: 1.925 ± 0.251
1.497PheMet: 1.497 ± 0.282
1.925PheAsn: 1.925 ± 0.317
1.39PhePro: 1.39 ± 0.3
0.802PheGln: 0.802 ± 0.212
3.048PheArg: 3.048 ± 0.514
3.797PheSer: 3.797 ± 0.524
2.086PheThr: 2.086 ± 0.263
2.941PheVal: 2.941 ± 0.493
0.481PheTrp: 0.481 ± 0.156
0.909PheTyr: 0.909 ± 0.206
0.0PheXaa: 0.0 ± 0.0
Gly
6.631GlyAla: 6.631 ± 1.044
0.749GlyCys: 0.749 ± 0.249
4.973GlyAsp: 4.973 ± 0.875
6.043GlyGlu: 6.043 ± 1.128
3.048GlyPhe: 3.048 ± 0.523
5.561GlyGly: 5.561 ± 0.752
1.123GlyHis: 1.123 ± 0.288
4.278GlyIle: 4.278 ± 0.548
5.027GlyLys: 5.027 ± 0.731
5.027GlyLeu: 5.027 ± 0.472
2.299GlyMet: 2.299 ± 0.345
3.048GlyAsn: 3.048 ± 0.402
4.225GlyPro: 4.225 ± 2.481
3.102GlyGln: 3.102 ± 0.431
4.813GlyArg: 4.813 ± 0.453
3.85GlySer: 3.85 ± 0.447
3.904GlyThr: 3.904 ± 0.501
4.866GlyVal: 4.866 ± 0.531
0.963GlyTrp: 0.963 ± 0.241
2.406GlyTyr: 2.406 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
1.979HisAla: 1.979 ± 0.242
0.053HisCys: 0.053 ± 0.054
0.695HisAsp: 0.695 ± 0.175
0.856HisGlu: 0.856 ± 0.271
0.963HisPhe: 0.963 ± 0.241
1.444HisGly: 1.444 ± 0.333
0.374HisHis: 0.374 ± 0.142
0.749HisIle: 0.749 ± 0.207
0.802HisLys: 0.802 ± 0.246
2.032HisLeu: 2.032 ± 0.482
0.481HisMet: 0.481 ± 0.139
0.856HisAsn: 0.856 ± 0.172
1.016HisPro: 1.016 ± 0.25
0.642HisGln: 0.642 ± 0.196
0.963HisArg: 0.963 ± 0.193
1.23HisSer: 1.23 ± 0.231
0.963HisThr: 0.963 ± 0.231
1.07HisVal: 1.07 ± 0.206
0.267HisTrp: 0.267 ± 0.145
0.856HisTyr: 0.856 ± 0.19
0.0HisXaa: 0.0 ± 0.0
Ile
5.348IleAla: 5.348 ± 0.541
0.695IleCys: 0.695 ± 0.195
3.048IleAsp: 3.048 ± 0.346
2.834IleGlu: 2.834 ± 0.478
1.23IlePhe: 1.23 ± 0.279
2.727IleGly: 2.727 ± 0.519
0.802IleHis: 0.802 ± 0.177
2.46IleIle: 2.46 ± 0.444
2.727IleLys: 2.727 ± 0.343
2.781IleLeu: 2.781 ± 0.463
1.016IleMet: 1.016 ± 0.229
2.995IleAsn: 2.995 ± 0.393
2.674IlePro: 2.674 ± 0.343
1.872IleGln: 1.872 ± 0.293
4.92IleArg: 4.92 ± 0.42
3.69IleSer: 3.69 ± 0.471
3.422IleThr: 3.422 ± 0.567
2.086IleVal: 2.086 ± 0.311
0.267IleTrp: 0.267 ± 0.11
1.39IleTyr: 1.39 ± 0.265
0.0IleXaa: 0.0 ± 0.0
Lys
6.417LysAla: 6.417 ± 0.537
0.642LysCys: 0.642 ± 0.226
2.888LysAsp: 2.888 ± 0.393
3.69LysGlu: 3.69 ± 0.456
1.551LysPhe: 1.551 ± 0.311
5.561LysGly: 5.561 ± 1.328
1.283LysHis: 1.283 ± 0.262
3.262LysIle: 3.262 ± 0.432
3.797LysLys: 3.797 ± 0.532
4.599LysLeu: 4.599 ± 0.441
1.23LysMet: 1.23 ± 0.269
3.636LysAsn: 3.636 ± 0.51
2.888LysPro: 2.888 ± 0.373
2.62LysGln: 2.62 ± 0.389
2.46LysArg: 2.46 ± 0.304
2.888LysSer: 2.888 ± 0.321
3.476LysThr: 3.476 ± 0.433
2.941LysVal: 2.941 ± 0.399
0.535LysTrp: 0.535 ± 0.18
1.604LysTyr: 1.604 ± 0.278
0.0LysXaa: 0.0 ± 0.0
Leu
8.663LeuAla: 8.663 ± 0.784
0.856LeuCys: 0.856 ± 0.241
3.904LeuAsp: 3.904 ± 0.524
3.797LeuGlu: 3.797 ± 0.573
2.513LeuPhe: 2.513 ± 0.369
4.652LeuGly: 4.652 ± 0.488
1.551LeuHis: 1.551 ± 0.27
3.209LeuIle: 3.209 ± 0.494
4.385LeuLys: 4.385 ± 0.463
5.936LeuLeu: 5.936 ± 0.519
1.872LeuMet: 1.872 ± 0.328
4.332LeuAsn: 4.332 ± 0.498
4.118LeuPro: 4.118 ± 0.473
3.316LeuGln: 3.316 ± 0.71
4.813LeuArg: 4.813 ± 0.477
5.775LeuSer: 5.775 ± 0.559
5.829LeuThr: 5.829 ± 0.552
4.599LeuVal: 4.599 ± 0.436
0.374LeuTrp: 0.374 ± 0.148
2.086LeuTyr: 2.086 ± 0.243
0.0LeuXaa: 0.0 ± 0.0
Met
2.781MetAla: 2.781 ± 0.361
0.16MetCys: 0.16 ± 0.096
1.283MetAsp: 1.283 ± 0.265
1.283MetGlu: 1.283 ± 0.208
0.749MetPhe: 0.749 ± 0.158
2.032MetGly: 2.032 ± 0.33
0.428MetHis: 0.428 ± 0.15
1.07MetIle: 1.07 ± 0.206
2.139MetLys: 2.139 ± 0.324
1.604MetLeu: 1.604 ± 0.283
1.07MetMet: 1.07 ± 0.29
1.872MetAsn: 1.872 ± 0.359
1.658MetPro: 1.658 ± 0.281
1.123MetGln: 1.123 ± 0.216
1.765MetArg: 1.765 ± 0.247
2.086MetSer: 2.086 ± 0.276
2.032MetThr: 2.032 ± 0.247
1.444MetVal: 1.444 ± 0.306
0.374MetTrp: 0.374 ± 0.156
0.695MetTyr: 0.695 ± 0.263
0.0MetXaa: 0.0 ± 0.0
Asn
4.973AsnAla: 4.973 ± 0.562
0.802AsnCys: 0.802 ± 0.232
2.353AsnAsp: 2.353 ± 0.412
2.674AsnGlu: 2.674 ± 0.356
1.283AsnPhe: 1.283 ± 0.292
3.743AsnGly: 3.743 ± 0.432
1.39AsnHis: 1.39 ± 0.322
2.46AsnIle: 2.46 ± 0.411
2.299AsnLys: 2.299 ± 0.335
3.262AsnLeu: 3.262 ± 0.388
1.283AsnMet: 1.283 ± 0.261
2.246AsnAsn: 2.246 ± 0.425
2.353AsnPro: 2.353 ± 0.319
1.872AsnGln: 1.872 ± 0.386
2.46AsnArg: 2.46 ± 0.51
2.62AsnSer: 2.62 ± 0.464
1.818AsnThr: 1.818 ± 0.241
2.781AsnVal: 2.781 ± 0.363
0.695AsnTrp: 0.695 ± 0.172
1.497AsnTyr: 1.497 ± 0.334
0.0AsnXaa: 0.0 ± 0.0
Pro
3.69ProAla: 3.69 ± 0.711
0.481ProCys: 0.481 ± 0.16
4.439ProAsp: 4.439 ± 0.57
4.813ProGlu: 4.813 ± 0.732
1.658ProPhe: 1.658 ± 0.29
3.422ProGly: 3.422 ± 0.68
0.321ProHis: 0.321 ± 0.121
0.963ProIle: 0.963 ± 0.255
2.834ProLys: 2.834 ± 0.786
2.995ProLeu: 2.995 ± 0.434
0.695ProMet: 0.695 ± 0.179
1.07ProAsn: 1.07 ± 0.251
1.337ProPro: 1.337 ± 0.312
2.139ProGln: 2.139 ± 0.586
2.513ProArg: 2.513 ± 0.393
2.727ProSer: 2.727 ± 0.374
2.567ProThr: 2.567 ± 0.422
4.706ProVal: 4.706 ± 0.531
0.535ProTrp: 0.535 ± 0.166
1.711ProTyr: 1.711 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
4.064GlnAla: 4.064 ± 0.67
0.695GlnCys: 0.695 ± 0.201
2.193GlnAsp: 2.193 ± 0.308
2.62GlnGlu: 2.62 ± 0.403
1.283GlnPhe: 1.283 ± 0.211
3.316GlnGly: 3.316 ± 0.882
0.963GlnHis: 0.963 ± 0.215
2.139GlnIle: 2.139 ± 0.361
2.941GlnLys: 2.941 ± 0.442
3.422GlnLeu: 3.422 ± 0.417
0.963GlnMet: 0.963 ± 0.266
1.658GlnAsn: 1.658 ± 0.346
2.032GlnPro: 2.032 ± 0.435
3.102GlnGln: 3.102 ± 0.88
2.513GlnArg: 2.513 ± 0.503
2.353GlnSer: 2.353 ± 0.286
1.872GlnThr: 1.872 ± 0.261
2.353GlnVal: 2.353 ± 0.383
0.695GlnTrp: 0.695 ± 0.172
1.337GlnTyr: 1.337 ± 0.256
0.0GlnXaa: 0.0 ± 0.0
Arg
4.118ArgAla: 4.118 ± 0.387
0.535ArgCys: 0.535 ± 0.191
3.636ArgAsp: 3.636 ± 0.52
5.348ArgGlu: 5.348 ± 0.705
3.102ArgPhe: 3.102 ± 0.42
4.92ArgGly: 4.92 ± 0.768
1.765ArgHis: 1.765 ± 0.241
3.155ArgIle: 3.155 ± 0.385
4.545ArgLys: 4.545 ± 0.528
5.134ArgLeu: 5.134 ± 0.4
1.765ArgMet: 1.765 ± 0.263
3.69ArgAsn: 3.69 ± 0.454
1.925ArgPro: 1.925 ± 0.333
2.406ArgGln: 2.406 ± 0.39
5.561ArgArg: 5.561 ± 0.639
3.529ArgSer: 3.529 ± 0.438
3.85ArgThr: 3.85 ± 0.477
3.797ArgVal: 3.797 ± 0.534
1.176ArgTrp: 1.176 ± 0.198
2.139ArgTyr: 2.139 ± 0.445
0.0ArgXaa: 0.0 ± 0.0
Ser
6.096SerAla: 6.096 ± 0.658
0.802SerCys: 0.802 ± 0.244
3.904SerAsp: 3.904 ± 0.429
4.225SerGlu: 4.225 ± 0.38
2.246SerPhe: 2.246 ± 0.331
5.561SerGly: 5.561 ± 0.596
0.963SerHis: 0.963 ± 0.278
2.353SerIle: 2.353 ± 0.299
2.246SerLys: 2.246 ± 0.329
5.775SerLeu: 5.775 ± 0.668
1.711SerMet: 1.711 ± 0.312
2.246SerAsn: 2.246 ± 0.336
3.102SerPro: 3.102 ± 0.401
2.513SerGln: 2.513 ± 0.375
4.599SerArg: 4.599 ± 0.522
2.834SerSer: 2.834 ± 0.519
3.369SerThr: 3.369 ± 0.499
4.225SerVal: 4.225 ± 0.572
0.695SerTrp: 0.695 ± 0.16
1.604SerTyr: 1.604 ± 0.325
0.0SerXaa: 0.0 ± 0.0
Thr
5.401ThrAla: 5.401 ± 0.446
0.321ThrCys: 0.321 ± 0.138
3.048ThrAsp: 3.048 ± 0.456
3.904ThrGlu: 3.904 ± 0.441
2.139ThrPhe: 2.139 ± 0.448
5.882ThrGly: 5.882 ± 0.708
0.856ThrHis: 0.856 ± 0.171
3.262ThrIle: 3.262 ± 0.44
2.995ThrLys: 2.995 ± 0.379
5.508ThrLeu: 5.508 ± 0.423
1.123ThrMet: 1.123 ± 0.203
1.604ThrAsn: 1.604 ± 0.329
3.422ThrPro: 3.422 ± 0.316
1.925ThrGln: 1.925 ± 0.307
2.46ThrArg: 2.46 ± 0.317
3.904ThrSer: 3.904 ± 0.501
3.69ThrThr: 3.69 ± 0.461
4.064ThrVal: 4.064 ± 0.568
1.123ThrTrp: 1.123 ± 0.24
1.39ThrTyr: 1.39 ± 0.286
0.0ThrXaa: 0.0 ± 0.0
Val
6.524ValAla: 6.524 ± 0.482
1.07ValCys: 1.07 ± 0.315
4.225ValAsp: 4.225 ± 0.54
3.743ValGlu: 3.743 ± 0.368
2.299ValPhe: 2.299 ± 0.372
3.316ValGly: 3.316 ± 0.448
0.749ValHis: 0.749 ± 0.209
3.422ValIle: 3.422 ± 0.433
3.583ValLys: 3.583 ± 0.465
5.561ValLeu: 5.561 ± 0.581
2.032ValMet: 2.032 ± 0.297
3.369ValAsn: 3.369 ± 0.452
3.048ValPro: 3.048 ± 0.442
2.032ValGln: 2.032 ± 0.475
4.599ValArg: 4.599 ± 0.899
4.332ValSer: 4.332 ± 0.589
4.973ValThr: 4.973 ± 0.615
4.118ValVal: 4.118 ± 0.512
0.749ValTrp: 0.749 ± 0.208
1.818ValTyr: 1.818 ± 0.311
0.0ValXaa: 0.0 ± 0.0
Trp
0.642TrpAla: 0.642 ± 0.19
0.16TrpCys: 0.16 ± 0.093
0.642TrpAsp: 0.642 ± 0.217
0.749TrpGlu: 0.749 ± 0.166
0.588TrpPhe: 0.588 ± 0.144
1.016TrpGly: 1.016 ± 0.268
0.267TrpHis: 0.267 ± 0.122
0.749TrpIle: 0.749 ± 0.22
0.802TrpLys: 0.802 ± 0.196
1.176TrpLeu: 1.176 ± 0.291
0.535TrpMet: 0.535 ± 0.149
0.588TrpAsn: 0.588 ± 0.21
0.642TrpPro: 0.642 ± 0.187
0.909TrpGln: 0.909 ± 0.209
1.07TrpArg: 1.07 ± 0.227
0.749TrpSer: 0.749 ± 0.184
0.535TrpThr: 0.535 ± 0.168
0.909TrpVal: 0.909 ± 0.197
0.374TrpTrp: 0.374 ± 0.15
0.214TrpTyr: 0.214 ± 0.08
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.888TyrAla: 2.888 ± 0.396
0.16TyrCys: 0.16 ± 0.093
1.711TyrAsp: 1.711 ± 0.325
1.283TyrGlu: 1.283 ± 0.284
1.283TyrPhe: 1.283 ± 0.268
2.62TyrGly: 2.62 ± 0.412
0.588TyrHis: 0.588 ± 0.183
1.658TyrIle: 1.658 ± 0.295
0.856TyrLys: 0.856 ± 0.193
1.925TyrLeu: 1.925 ± 0.309
0.802TyrMet: 0.802 ± 0.196
1.497TyrAsn: 1.497 ± 0.273
1.497TyrPro: 1.497 ± 0.275
1.551TyrGln: 1.551 ± 0.286
2.246TyrArg: 2.246 ± 0.394
1.551TyrSer: 1.551 ± 0.25
1.497TyrThr: 1.497 ± 0.391
2.086TyrVal: 2.086 ± 0.316
0.749TyrTrp: 0.749 ± 0.197
1.23TyrTyr: 1.23 ± 0.221
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (18701 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski