Salmonella phage SPFM13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Seoulvirus; unclassified Seoulvirus

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4E0W838|A0A4E0W838_9CAUD Uncharacterized protein OS=Salmonella phage SPFM13 OX=2528683 GN=SPFM13_00238 PE=4 SV=1
MM1 pKa = 7.44NLFEE5 pKa = 5.99HH6 pKa = 7.47PDD8 pKa = 3.4DD9 pKa = 5.05ALPCRR14 pKa = 11.84SAGIGEE20 pKa = 4.56DD21 pKa = 5.4FITTDD26 pKa = 3.24NVFGLCHH33 pKa = 7.09LFAGLFVFLIEE44 pKa = 5.09FDD46 pKa = 4.0FAA48 pKa = 5.74

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4E0W7R1|A0A4E0W7R1_9CAUD Putative radical SAM superfamily protein OS=Salmonella phage SPFM13 OX=2528683 GN=SPFM13_00118 PE=3 SV=1
MM1 pKa = 7.68LKK3 pKa = 10.08PRR5 pKa = 11.84KK6 pKa = 8.82QKK8 pKa = 10.27RR9 pKa = 11.84LQFVKK14 pKa = 10.09IVHH17 pKa = 6.15PEE19 pKa = 3.61SRR21 pKa = 11.84RR22 pKa = 11.84VTGGRR27 pKa = 11.84LYY29 pKa = 10.71VGKK32 pKa = 9.83HH33 pKa = 4.83RR34 pKa = 11.84SRR36 pKa = 11.84LITWSAMANVATQLQAMVEE55 pKa = 4.22DD56 pKa = 4.41RR57 pKa = 11.84CQLKK61 pKa = 10.88GDD63 pKa = 4.38FFQEE67 pKa = 3.81KK68 pKa = 9.8KK69 pKa = 9.86PYY71 pKa = 9.94RR72 pKa = 11.84DD73 pKa = 2.73IFRR76 pKa = 11.84TLKK79 pKa = 10.67ACWVLSWVPGRR90 pKa = 11.84EE91 pKa = 3.51QLYY94 pKa = 9.72FLRR97 pKa = 11.84KK98 pKa = 9.52FPRR101 pKa = 11.84PMPKK105 pKa = 10.29EE106 pKa = 4.21NISVFLDD113 pKa = 3.03KK114 pKa = 10.99LKK116 pKa = 10.56RR117 pKa = 11.84FNGIGINANKK127 pKa = 9.11EE128 pKa = 4.08QVSATVAARR137 pKa = 11.84KK138 pKa = 9.27LLRR141 pKa = 11.84DD142 pKa = 3.73YY143 pKa = 11.37VADD146 pKa = 3.67

Molecular weight: 17.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

261

0

261

74714

30

2373

286.3

32.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.76 ± 0.162

0.92 ± 0.055

6.324 ± 0.094

6.362 ± 0.141

3.879 ± 0.074

6.667 ± 0.173

1.961 ± 0.074

5.628 ± 0.09

5.211 ± 0.126

9.061 ± 0.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.702 ± 0.073

4.813 ± 0.086

4.648 ± 0.103

3.614 ± 0.085

5.545 ± 0.122

5.629 ± 0.117

6.582 ± 0.162

7.076 ± 0.106

1.475 ± 0.068

4.142 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski