Luminiphilus syltensis NOR5-1B

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Luminiphilus; Luminiphilus syltensis

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2923 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8KTG7|B8KTG7_9GAMM FOG: CBS domain protein OS=Luminiphilus syltensis NOR5-1B OX=565045 GN=NOR51B_1511 PE=4 SV=1
MM1 pKa = 7.2GQFDD5 pKa = 4.09SEE7 pKa = 4.58ANAMRR12 pKa = 11.84YY13 pKa = 7.79VAEE16 pKa = 4.47GAFGGPLSEE25 pKa = 4.21TVAARR30 pKa = 11.84AAFRR34 pKa = 11.84YY35 pKa = 9.51SKK37 pKa = 10.52QDD39 pKa = 3.25PYY41 pKa = 11.71LKK43 pKa = 10.43NLYY46 pKa = 10.78SNDD49 pKa = 3.55DD50 pKa = 3.57QLPFLGDD57 pKa = 3.76PGPGAGADD65 pKa = 4.03LGDD68 pKa = 5.55DD69 pKa = 3.61DD70 pKa = 5.49TIAARR75 pKa = 11.84LRR77 pKa = 11.84FAFTPSDD84 pKa = 4.2DD85 pKa = 4.04LRR87 pKa = 11.84MDD89 pKa = 4.13LSFNYY94 pKa = 9.94SDD96 pKa = 4.5SEE98 pKa = 4.76VNTGPYY104 pKa = 9.3QSKK107 pKa = 8.57STLGIATEE115 pKa = 4.26VNGNLEE121 pKa = 4.9LINVVNTPADD131 pKa = 3.88EE132 pKa = 4.37TRR134 pKa = 11.84LTAVVDD140 pKa = 3.66ADD142 pKa = 4.4GNDD145 pKa = 3.24TGLDD149 pKa = 3.29GGADD153 pKa = 3.46IEE155 pKa = 5.3DD156 pKa = 4.14GDD158 pKa = 4.33SFLPGGGGGLPGRR171 pKa = 11.84LAPGADD177 pKa = 3.17FFGYY181 pKa = 10.46LDD183 pKa = 4.33PDD185 pKa = 4.03GDD187 pKa = 4.54DD188 pKa = 3.71FTFSGDD194 pKa = 3.28FAFRR198 pKa = 11.84DD199 pKa = 3.47QGSVEE204 pKa = 3.79NSGVNFRR211 pKa = 11.84LIYY214 pKa = 10.35DD215 pKa = 4.37LSDD218 pKa = 3.11TTTFTSITDD227 pKa = 3.47YY228 pKa = 11.4KK229 pKa = 11.0DD230 pKa = 3.37YY231 pKa = 11.31EE232 pKa = 4.16KK233 pKa = 11.32LLFIDD238 pKa = 3.91VDD240 pKa = 3.87AAPVNQLANYY250 pKa = 9.4AGVDD254 pKa = 3.34ASSFTQEE261 pKa = 3.66FRR263 pKa = 11.84LSGEE267 pKa = 4.31TEE269 pKa = 3.63RR270 pKa = 11.84SRR272 pKa = 11.84WVAGLYY278 pKa = 9.63YY279 pKa = 10.79LNIDD283 pKa = 3.87SEE285 pKa = 4.69SDD287 pKa = 3.23NGLKK291 pKa = 10.61GPQNSFADD299 pKa = 3.79VFGGSPIDD307 pKa = 3.65VGTVANLEE315 pKa = 4.11TDD317 pKa = 3.32SYY319 pKa = 11.58SIFGQYY325 pKa = 10.42EE326 pKa = 4.0YY327 pKa = 11.34DD328 pKa = 3.9FSDD331 pKa = 3.66KK332 pKa = 10.43LTVIAGLRR340 pKa = 11.84AMQEE344 pKa = 3.94EE345 pKa = 4.81KK346 pKa = 11.11DD347 pKa = 3.62FGLVLGVAPSDD358 pKa = 3.47GSRR361 pKa = 11.84VVNSSPCFSGQLDD374 pKa = 3.55ACFPGTGVSYY384 pKa = 11.23EE385 pKa = 4.53DD386 pKa = 4.61DD387 pKa = 3.65FSEE390 pKa = 4.59NFWAGKK396 pKa = 9.29LQLTYY401 pKa = 10.83DD402 pKa = 3.6VSDD405 pKa = 4.63DD406 pKa = 3.48LMLYY410 pKa = 10.42AGVNRR415 pKa = 11.84GVKK418 pKa = 9.82AGSYY422 pKa = 8.46NAPLLGAYY430 pKa = 9.47FGSGGDD436 pKa = 3.12AGLPYY441 pKa = 10.62DD442 pKa = 4.24EE443 pKa = 5.6EE444 pKa = 4.56VLTSYY449 pKa = 10.91EE450 pKa = 4.28GGFKK454 pKa = 9.91ATLGGGTTRR463 pKa = 11.84INATAFYY470 pKa = 10.29YY471 pKa = 10.14DD472 pKa = 3.65YY473 pKa = 11.36KK474 pKa = 10.99DD475 pKa = 3.49YY476 pKa = 11.31QAFLFVGVGGVVTNQDD492 pKa = 2.77ADD494 pKa = 3.59NYY496 pKa = 10.13GVEE499 pKa = 5.22LEE501 pKa = 4.3IQTTPVDD508 pKa = 3.92GLDD511 pKa = 4.27LIISGAYY518 pKa = 9.65FDD520 pKa = 4.68ATVKK524 pKa = 10.68DD525 pKa = 3.66VSLRR529 pKa = 11.84NGSPLPPSDD538 pKa = 4.27VDD540 pKa = 3.28PTYY543 pKa = 11.16APEE546 pKa = 4.28LQATALARR554 pKa = 11.84YY555 pKa = 9.85SFDD558 pKa = 3.51AFGGLMAIQGDD569 pKa = 3.38ISYY572 pKa = 11.0SDD574 pKa = 3.5EE575 pKa = 4.09YY576 pKa = 11.39YY577 pKa = 10.95YY578 pKa = 11.26NLRR581 pKa = 11.84NFDD584 pKa = 3.51ADD586 pKa = 3.53KK587 pKa = 10.64FDD589 pKa = 4.43SYY591 pKa = 11.67VVANAQLSYY600 pKa = 11.38AVDD603 pKa = 3.28DD604 pKa = 4.14WLATLAVRR612 pKa = 11.84NLTDD616 pKa = 3.41EE617 pKa = 4.21RR618 pKa = 11.84VGIQGFDD625 pKa = 3.25LATLCGCNEE634 pKa = 4.06VAYY637 pKa = 9.59KK638 pKa = 10.17APRR641 pKa = 11.84YY642 pKa = 9.67FSFGLRR648 pKa = 11.84KK649 pKa = 10.23DD650 pKa = 3.58FF651 pKa = 4.77

Molecular weight: 70.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8KXL6|B8KXL6_9GAMM Long-chain-fatty-acid--CoA ligase OS=Luminiphilus syltensis NOR5-1B OX=565045 GN=fadD PE=4 SV=1
MM1 pKa = 7.56NFRR4 pKa = 11.84KK5 pKa = 10.21ALDD8 pKa = 3.44AKK10 pKa = 9.75IQRR13 pKa = 11.84EE14 pKa = 3.39IAYY17 pKa = 9.77RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84FRR22 pKa = 11.84RR23 pKa = 11.84WLVYY27 pKa = 9.99FSLSCLCWTTGMAIPFFTLNTSGSFRR53 pKa = 11.84GALLAIYY60 pKa = 9.18FAGVALLVVWSDD72 pKa = 3.08RR73 pKa = 11.84KK74 pKa = 10.47SRR76 pKa = 11.84QSNDD80 pKa = 3.19DD81 pKa = 3.15

Molecular weight: 9.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2923

0

2923

948102

30

1947

324.4

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.65 ± 0.051

1.006 ± 0.015

6.284 ± 0.038

6.242 ± 0.043

3.779 ± 0.031

8.132 ± 0.041

2.085 ± 0.026

5.32 ± 0.028

3.058 ± 0.038

10.252 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.021

3.09 ± 0.028

4.819 ± 0.029

3.608 ± 0.024

6.336 ± 0.043

6.223 ± 0.036

5.4 ± 0.041

7.365 ± 0.038

1.354 ± 0.019

2.612 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski