Rubripirellula obstinata

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rubripirellula

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5669 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B1CBR1|A0A5B1CBR1_9BACT Uncharacterized protein OS=Rubripirellula obstinata OX=406547 GN=LF1_53420 PE=4 SV=1
MM1 pKa = 7.59SYY3 pKa = 10.03YY4 pKa = 10.59VRR6 pKa = 11.84YY7 pKa = 9.65ICTDD11 pKa = 3.39PRR13 pKa = 11.84PVSLDD18 pKa = 3.42DD19 pKa = 4.54LRR21 pKa = 11.84LGLATVDD28 pKa = 3.62PQYY31 pKa = 11.34SIVDD35 pKa = 3.95DD36 pKa = 4.07EE37 pKa = 4.67LHH39 pKa = 7.06FDD41 pKa = 3.86GEE43 pKa = 4.89LYY45 pKa = 10.77GQLDD49 pKa = 3.56VNARR53 pKa = 11.84GTEE56 pKa = 4.0LCDD59 pKa = 3.52EE60 pKa = 4.76EE61 pKa = 6.24LDD63 pKa = 3.78EE64 pKa = 4.78LQEE67 pKa = 4.06FLEE70 pKa = 4.72DD71 pKa = 3.92AEE73 pKa = 4.91SGDD76 pKa = 3.62TQRR79 pKa = 11.84VVDD82 pKa = 4.67CLTNATSIIAIQVLWQGRR100 pKa = 11.84EE101 pKa = 3.92PEE103 pKa = 4.1PTLEE107 pKa = 3.56RR108 pKa = 11.84VYY110 pKa = 11.26AVLEE114 pKa = 3.8WLLANRR120 pKa = 11.84SGIAQVDD127 pKa = 3.68DD128 pKa = 3.52NGYY131 pKa = 10.0YY132 pKa = 10.44DD133 pKa = 4.53SNGQVVAVSS142 pKa = 3.41

Molecular weight: 15.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B1CHL8|A0A5B1CHL8_9BACT Iron-sulfur cluster carrier protein OS=Rubripirellula obstinata OX=406547 GN=ylxH PE=3 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84LVLVISVVLLIVSAFVSWQFGNDD27 pKa = 2.81STARR31 pKa = 11.84FAASASGRR39 pKa = 11.84IGLVMAALWLAWPSLKK55 pKa = 10.36RR56 pKa = 11.84PAAWLPPGIAVGLVVALAVVAAQPRR81 pKa = 11.84LIIVAVPAIGILFTLASVVRR101 pKa = 11.84SFRR104 pKa = 11.84SS105 pKa = 3.17

Molecular weight: 11.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5669

0

5669

1934098

29

10743

341.2

37.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.369 ± 0.037

1.18 ± 0.023

6.622 ± 0.052

5.777 ± 0.038

3.764 ± 0.023

7.671 ± 0.053

2.071 ± 0.023

5.432 ± 0.024

3.788 ± 0.051

9.238 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.363 ± 0.03

3.454 ± 0.043

4.902 ± 0.034

4.018 ± 0.027

6.393 ± 0.055

7.243 ± 0.04

5.877 ± 0.079

7.223 ± 0.033

1.371 ± 0.016

2.246 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski