Lactobacillus oryzae JCM 18671

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Secundilactobacillus; Secundilactobacillus oryzae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1890 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A081BJ18|A0A081BJ18_9LACO DEAD-box ATP-dependent RNA helicase CshB OS=Lactobacillus oryzae JCM 18671 OX=1291743 GN=cshB PE=3 SV=1
MM1 pKa = 7.05VNYY4 pKa = 9.69YY5 pKa = 10.32NKK7 pKa = 10.38SEE9 pKa = 4.15GPHH12 pKa = 5.31MSEE15 pKa = 4.4EE16 pKa = 4.21NQQITLVDD24 pKa = 3.42EE25 pKa = 5.21DD26 pKa = 4.59GNEE29 pKa = 3.81EE30 pKa = 4.17LYY32 pKa = 10.88EE33 pKa = 3.94VLFTFKK39 pKa = 10.94SPDD42 pKa = 3.33YY43 pKa = 8.9GKK45 pKa = 10.66SYY47 pKa = 11.08ILMYY51 pKa = 10.08PAGKK55 pKa = 10.34ADD57 pKa = 3.99DD58 pKa = 4.43EE59 pKa = 4.5EE60 pKa = 5.12VDD62 pKa = 3.24IQAYY66 pKa = 7.74QLPDD70 pKa = 3.54KK71 pKa = 10.48DD72 pKa = 5.31DD73 pKa = 4.17PANPSGGDD81 pKa = 3.64LLPIEE86 pKa = 5.34SDD88 pKa = 3.84EE89 pKa = 4.12EE90 pKa = 4.04WDD92 pKa = 3.66MVEE95 pKa = 5.11SMLNTFLNGGGTDD108 pKa = 5.29AFDD111 pKa = 4.03QDD113 pKa = 3.02WGDD116 pKa = 3.57PEE118 pKa = 4.2KK119 pKa = 11.17

Molecular weight: 13.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A081BIH9|A0A081BIH9_9LACO Transport permease protein OS=Lactobacillus oryzae JCM 18671 OX=1291743 GN=LOSG293_130360 PE=3 SV=1
MM1 pKa = 7.4NNQPNTYY8 pKa = 7.0VTKK11 pKa = 9.97PVFVFGFPIVMAIFQALMCIINDD34 pKa = 3.43TRR36 pKa = 11.84TKK38 pKa = 10.49DD39 pKa = 3.37TVDD42 pKa = 3.54PSLTAVFKK50 pKa = 11.11SIVPFLSNLLYY61 pKa = 10.44TVTILFALGHH71 pKa = 6.2LVNIRR76 pKa = 11.84SVAMIALGLISLLVGGFSWNRR97 pKa = 11.84RR98 pKa = 11.84LVSEE102 pKa = 4.39KK103 pKa = 10.1TSAANAIAIKK113 pKa = 10.07RR114 pKa = 11.84ANHH117 pKa = 5.74LVVLSLIVSGLLMLGSVFLPASYY140 pKa = 10.52SVAAIGVFIVLVLLSAWQLTRR161 pKa = 11.84VRR163 pKa = 11.84NRR165 pKa = 11.84EE166 pKa = 3.68

Molecular weight: 18.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1890

0

1890

534300

35

1585

282.7

31.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.106 ± 0.057

0.365 ± 0.012

5.962 ± 0.061

5.998 ± 0.069

4.179 ± 0.042

6.704 ± 0.054

2.072 ± 0.027

6.885 ± 0.054

6.414 ± 0.059

9.593 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.026

4.755 ± 0.034

3.571 ± 0.028

4.51 ± 0.043

4.162 ± 0.044

6.036 ± 0.056

6.325 ± 0.048

7.27 ± 0.051

0.992 ± 0.023

3.41 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski