Amino acid dipepetide frequency for Flavobacterium phage V182

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.001AlaAla: 2.001 ± 0.717
0.6AlaCys: 0.6 ± 0.188
2.601AlaAsp: 2.601 ± 0.342
3.268AlaGlu: 3.268 ± 0.584
2.534AlaPhe: 2.534 ± 0.565
2.934AlaGly: 2.934 ± 0.575
0.4AlaHis: 0.4 ± 0.176
4.001AlaIle: 4.001 ± 0.495
5.001AlaLys: 5.001 ± 0.81
3.734AlaLeu: 3.734 ± 0.702
1.134AlaMet: 1.134 ± 0.333
4.068AlaAsn: 4.068 ± 0.66
1.467AlaPro: 1.467 ± 0.332
1.8AlaGln: 1.8 ± 0.359
2.067AlaArg: 2.067 ± 0.362
4.201AlaSer: 4.201 ± 0.592
3.401AlaThr: 3.401 ± 0.623
3.201AlaVal: 3.201 ± 0.582
0.667AlaTrp: 0.667 ± 0.212
2.401AlaTyr: 2.401 ± 0.486
0.0AlaXaa: 0.0 ± 0.0
Cys
0.4CysAla: 0.4 ± 0.213
0.133CysCys: 0.133 ± 0.093
0.867CysAsp: 0.867 ± 0.215
0.533CysGlu: 0.533 ± 0.167
0.667CysPhe: 0.667 ± 0.238
0.333CysGly: 0.333 ± 0.136
0.2CysHis: 0.2 ± 0.126
0.734CysIle: 0.734 ± 0.228
1.134CysLys: 1.134 ± 0.285
1.0CysLeu: 1.0 ± 0.285
0.133CysMet: 0.133 ± 0.087
0.533CysAsn: 0.533 ± 0.191
0.2CysPro: 0.2 ± 0.091
0.2CysGln: 0.2 ± 0.099
0.4CysArg: 0.4 ± 0.175
0.533CysSer: 0.533 ± 0.2
0.8CysThr: 0.8 ± 0.245
0.667CysVal: 0.667 ± 0.244
0.0CysTrp: 0.0 ± 0.0
0.333CysTyr: 0.333 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
4.201AspAla: 4.201 ± 0.504
0.667AspCys: 0.667 ± 0.223
2.934AspAsp: 2.934 ± 0.418
4.134AspGlu: 4.134 ± 0.507
3.668AspPhe: 3.668 ± 0.458
3.134AspGly: 3.134 ± 0.404
0.6AspHis: 0.6 ± 0.2
4.134AspIle: 4.134 ± 0.449
3.668AspLys: 3.668 ± 0.495
7.069AspLeu: 7.069 ± 0.624
0.867AspMet: 0.867 ± 0.248
4.401AspAsn: 4.401 ± 0.683
1.534AspPro: 1.534 ± 0.316
1.267AspGln: 1.267 ± 0.312
1.4AspArg: 1.4 ± 0.307
3.734AspSer: 3.734 ± 0.58
2.401AspThr: 2.401 ± 0.435
3.668AspVal: 3.668 ± 0.53
0.934AspTrp: 0.934 ± 0.269
3.067AspTyr: 3.067 ± 0.423
0.0AspXaa: 0.0 ± 0.0
Glu
3.401GluAla: 3.401 ± 0.521
0.4GluCys: 0.4 ± 0.172
2.801GluAsp: 2.801 ± 0.421
4.535GluGlu: 4.535 ± 0.754
3.601GluPhe: 3.601 ± 0.447
2.201GluGly: 2.201 ± 0.462
1.134GluHis: 1.134 ± 0.289
5.868GluIle: 5.868 ± 0.56
7.069GluLys: 7.069 ± 0.95
7.935GluLeu: 7.935 ± 0.821
2.534GluMet: 2.534 ± 0.458
5.468GluAsn: 5.468 ± 0.792
1.467GluPro: 1.467 ± 0.333
2.601GluGln: 2.601 ± 0.429
3.134GluArg: 3.134 ± 0.526
4.601GluSer: 4.601 ± 0.579
4.201GluThr: 4.201 ± 0.575
3.334GluVal: 3.334 ± 0.515
0.8GluTrp: 0.8 ± 0.253
2.667GluTyr: 2.667 ± 0.478
0.0GluXaa: 0.0 ± 0.0
Phe
1.734PheAla: 1.734 ± 0.322
0.667PheCys: 0.667 ± 0.235
3.868PheAsp: 3.868 ± 0.501
4.001PheGlu: 4.001 ± 0.48
2.067PhePhe: 2.067 ± 0.355
3.334PheGly: 3.334 ± 0.581
0.6PheHis: 0.6 ± 0.163
3.868PheIle: 3.868 ± 0.494
5.135PheLys: 5.135 ± 0.585
3.734PheLeu: 3.734 ± 0.42
1.134PheMet: 1.134 ± 0.258
3.468PheAsn: 3.468 ± 0.457
1.134PhePro: 1.134 ± 0.203
1.334PheGln: 1.334 ± 0.238
1.534PheArg: 1.534 ± 0.344
2.801PheSer: 2.801 ± 0.515
2.867PheThr: 2.867 ± 0.427
2.601PheVal: 2.601 ± 0.395
0.533PheTrp: 0.533 ± 0.167
2.201PheTyr: 2.201 ± 0.37
0.0PheXaa: 0.0 ± 0.0
Gly
2.801GlyAla: 2.801 ± 0.535
0.4GlyCys: 0.4 ± 0.14
3.868GlyAsp: 3.868 ± 0.604
3.401GlyGlu: 3.401 ± 0.514
3.401GlyPhe: 3.401 ± 0.448
4.201GlyGly: 4.201 ± 0.62
0.2GlyHis: 0.2 ± 0.103
4.334GlyIle: 4.334 ± 0.561
4.735GlyLys: 4.735 ± 0.582
4.601GlyLeu: 4.601 ± 0.555
0.6GlyMet: 0.6 ± 0.237
3.468GlyAsn: 3.468 ± 0.463
0.533GlyPro: 0.533 ± 0.16
1.734GlyGln: 1.734 ± 0.283
2.001GlyArg: 2.001 ± 0.367
4.134GlySer: 4.134 ± 0.572
3.067GlyThr: 3.067 ± 0.483
4.001GlyVal: 4.001 ± 0.548
0.734GlyTrp: 0.734 ± 0.209
2.734GlyTyr: 2.734 ± 0.462
0.0GlyXaa: 0.0 ± 0.0
His
0.267HisAla: 0.267 ± 0.144
0.267HisCys: 0.267 ± 0.165
0.734HisAsp: 0.734 ± 0.242
0.867HisGlu: 0.867 ± 0.203
0.734HisPhe: 0.734 ± 0.22
1.0HisGly: 1.0 ± 0.337
0.133HisHis: 0.133 ± 0.098
1.334HisIle: 1.334 ± 0.301
1.134HisLys: 1.134 ± 0.241
0.867HisLeu: 0.867 ± 0.235
0.133HisMet: 0.133 ± 0.088
1.2HisAsn: 1.2 ± 0.338
0.734HisPro: 0.734 ± 0.178
0.533HisGln: 0.533 ± 0.191
0.867HisArg: 0.867 ± 0.236
0.734HisSer: 0.734 ± 0.212
0.533HisThr: 0.533 ± 0.181
0.6HisVal: 0.6 ± 0.224
0.4HisTrp: 0.4 ± 0.183
0.533HisTyr: 0.533 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
4.868IleAla: 4.868 ± 0.771
0.4IleCys: 0.4 ± 0.154
5.268IleAsp: 5.268 ± 0.546
6.335IleGlu: 6.335 ± 0.683
2.734IlePhe: 2.734 ± 0.421
4.735IleGly: 4.735 ± 0.612
1.134IleHis: 1.134 ± 0.245
6.535IleIle: 6.535 ± 0.674
7.935IleLys: 7.935 ± 0.836
6.402IleLeu: 6.402 ± 0.614
1.534IleMet: 1.534 ± 0.299
5.601IleAsn: 5.601 ± 0.667
2.801IlePro: 2.801 ± 0.473
3.801IleGln: 3.801 ± 0.435
2.401IleArg: 2.401 ± 0.384
4.935IleSer: 4.935 ± 0.651
4.735IleThr: 4.735 ± 0.594
4.268IleVal: 4.268 ± 0.502
0.667IleTrp: 0.667 ± 0.217
2.934IleTyr: 2.934 ± 0.483
0.0IleXaa: 0.0 ± 0.0
Lys
5.268LysAla: 5.268 ± 0.881
0.8LysCys: 0.8 ± 0.273
4.668LysAsp: 4.668 ± 0.549
6.868LysGlu: 6.868 ± 0.808
3.334LysPhe: 3.334 ± 0.56
5.668LysGly: 5.668 ± 0.55
1.667LysHis: 1.667 ± 0.338
6.468LysIle: 6.468 ± 0.683
8.202LysLys: 8.202 ± 0.946
8.469LysLeu: 8.469 ± 0.657
2.601LysMet: 2.601 ± 0.459
6.468LysAsn: 6.468 ± 0.569
3.401LysPro: 3.401 ± 0.556
3.534LysGln: 3.534 ± 0.524
4.201LysArg: 4.201 ± 0.557
5.935LysSer: 5.935 ± 0.611
5.735LysThr: 5.735 ± 0.773
4.668LysVal: 4.668 ± 0.57
1.067LysTrp: 1.067 ± 0.223
4.401LysTyr: 4.401 ± 0.584
0.0LysXaa: 0.0 ± 0.0
Leu
4.735LeuAla: 4.735 ± 0.607
0.8LeuCys: 0.8 ± 0.254
5.335LeuAsp: 5.335 ± 0.631
8.069LeuGlu: 8.069 ± 0.712
4.001LeuPhe: 4.001 ± 0.558
4.801LeuGly: 4.801 ± 0.597
1.0LeuHis: 1.0 ± 0.255
7.535LeuIle: 7.535 ± 0.734
8.536LeuLys: 8.536 ± 0.76
6.335LeuLeu: 6.335 ± 0.646
2.201LeuMet: 2.201 ± 0.399
6.135LeuAsn: 6.135 ± 0.697
3.067LeuPro: 3.067 ± 0.418
2.801LeuGln: 2.801 ± 0.338
3.334LeuArg: 3.334 ± 0.436
5.935LeuSer: 5.935 ± 0.775
5.335LeuThr: 5.335 ± 0.7
5.335LeuVal: 5.335 ± 0.538
0.867LeuTrp: 0.867 ± 0.269
2.601LeuTyr: 2.601 ± 0.371
0.0LeuXaa: 0.0 ± 0.0
Met
1.8MetAla: 1.8 ± 0.339
0.267MetCys: 0.267 ± 0.143
1.067MetAsp: 1.067 ± 0.38
0.8MetGlu: 0.8 ± 0.235
0.8MetPhe: 0.8 ± 0.226
1.0MetGly: 1.0 ± 0.252
0.133MetHis: 0.133 ± 0.096
2.067MetIle: 2.067 ± 0.408
2.534MetLys: 2.534 ± 0.449
1.467MetLeu: 1.467 ± 0.294
0.067MetMet: 0.067 ± 0.059
1.534MetAsn: 1.534 ± 0.34
0.6MetPro: 0.6 ± 0.257
1.4MetGln: 1.4 ± 0.312
1.134MetArg: 1.134 ± 0.336
1.4MetSer: 1.4 ± 0.328
1.0MetThr: 1.0 ± 0.271
0.934MetVal: 0.934 ± 0.261
0.267MetTrp: 0.267 ± 0.133
1.0MetTyr: 1.0 ± 0.289
0.0MetXaa: 0.0 ± 0.0
Asn
3.334AsnAla: 3.334 ± 0.557
1.0AsnCys: 1.0 ± 0.24
3.868AsnAsp: 3.868 ± 0.539
5.935AsnGlu: 5.935 ± 0.669
3.401AsnPhe: 3.401 ± 0.593
4.735AsnGly: 4.735 ± 0.72
1.134AsnHis: 1.134 ± 0.228
5.735AsnIle: 5.735 ± 0.591
6.335AsnLys: 6.335 ± 0.796
6.668AsnLeu: 6.668 ± 0.66
1.0AsnMet: 1.0 ± 0.237
6.935AsnAsn: 6.935 ± 0.807
2.267AsnPro: 2.267 ± 0.417
2.467AsnGln: 2.467 ± 0.484
2.201AsnArg: 2.201 ± 0.311
5.401AsnSer: 5.401 ± 0.662
4.334AsnThr: 4.334 ± 0.507
3.534AsnVal: 3.534 ± 0.69
0.4AsnTrp: 0.4 ± 0.159
3.067AsnTyr: 3.067 ± 0.391
0.0AsnXaa: 0.0 ± 0.0
Pro
1.267ProAla: 1.267 ± 0.38
0.4ProCys: 0.4 ± 0.131
2.334ProAsp: 2.334 ± 0.481
1.8ProGlu: 1.8 ± 0.367
1.6ProPhe: 1.6 ± 0.284
0.533ProGly: 0.533 ± 0.208
0.6ProHis: 0.6 ± 0.151
2.134ProIle: 2.134 ± 0.366
2.934ProLys: 2.934 ± 0.485
2.067ProLeu: 2.067 ± 0.391
0.2ProMet: 0.2 ± 0.148
2.267ProAsn: 2.267 ± 0.281
0.4ProPro: 0.4 ± 0.148
1.134ProGln: 1.134 ± 0.359
0.667ProArg: 0.667 ± 0.249
1.867ProSer: 1.867 ± 0.402
2.334ProThr: 2.334 ± 0.413
2.201ProVal: 2.201 ± 0.427
0.2ProTrp: 0.2 ± 0.12
1.4ProTyr: 1.4 ± 0.345
0.0ProXaa: 0.0 ± 0.0
Gln
1.867GlnAla: 1.867 ± 0.4
0.2GlnCys: 0.2 ± 0.123
1.6GlnAsp: 1.6 ± 0.291
2.601GlnGlu: 2.601 ± 0.438
1.6GlnPhe: 1.6 ± 0.274
2.067GlnGly: 2.067 ± 0.308
0.667GlnHis: 0.667 ± 0.316
2.734GlnIle: 2.734 ± 0.34
3.001GlnLys: 3.001 ± 0.491
3.868GlnLeu: 3.868 ± 0.426
1.2GlnMet: 1.2 ± 0.304
2.334GlnAsn: 2.334 ± 0.404
1.0GlnPro: 1.0 ± 0.276
1.934GlnGln: 1.934 ± 0.542
1.134GlnArg: 1.134 ± 0.286
2.801GlnSer: 2.801 ± 0.495
1.8GlnThr: 1.8 ± 0.292
1.467GlnVal: 1.467 ± 0.309
0.4GlnTrp: 0.4 ± 0.165
1.734GlnTyr: 1.734 ± 0.274
0.0GlnXaa: 0.0 ± 0.0
Arg
1.6ArgAla: 1.6 ± 0.297
0.4ArgCys: 0.4 ± 0.168
2.134ArgAsp: 2.134 ± 0.493
2.201ArgGlu: 2.201 ± 0.405
2.401ArgPhe: 2.401 ± 0.497
1.2ArgGly: 1.2 ± 0.244
0.734ArgHis: 0.734 ± 0.201
3.201ArgIle: 3.201 ± 0.412
3.668ArgLys: 3.668 ± 0.564
3.468ArgLeu: 3.468 ± 0.565
0.667ArgMet: 0.667 ± 0.189
2.267ArgAsn: 2.267 ± 0.404
0.467ArgPro: 0.467 ± 0.159
1.2ArgGln: 1.2 ± 0.29
0.934ArgArg: 0.934 ± 0.262
1.734ArgSer: 1.734 ± 0.348
2.134ArgThr: 2.134 ± 0.372
2.801ArgVal: 2.801 ± 0.435
0.267ArgTrp: 0.267 ± 0.136
1.8ArgTyr: 1.8 ± 0.379
0.0ArgXaa: 0.0 ± 0.0
Ser
3.201SerAla: 3.201 ± 0.587
0.4SerCys: 0.4 ± 0.156
3.334SerAsp: 3.334 ± 0.387
4.134SerGlu: 4.134 ± 0.483
3.201SerPhe: 3.201 ± 0.46
4.334SerGly: 4.334 ± 0.538
0.867SerHis: 0.867 ± 0.223
5.735SerIle: 5.735 ± 0.664
7.802SerLys: 7.802 ± 0.745
6.135SerLeu: 6.135 ± 0.685
1.667SerMet: 1.667 ± 0.379
4.334SerAsn: 4.334 ± 0.653
1.334SerPro: 1.334 ± 0.304
2.534SerGln: 2.534 ± 0.402
1.934SerArg: 1.934 ± 0.345
2.934SerSer: 2.934 ± 0.54
3.734SerThr: 3.734 ± 0.469
4.668SerVal: 4.668 ± 0.468
0.8SerTrp: 0.8 ± 0.183
3.001SerTyr: 3.001 ± 0.442
0.0SerXaa: 0.0 ± 0.0
Thr
3.801ThrAla: 3.801 ± 0.783
0.4ThrCys: 0.4 ± 0.228
3.334ThrAsp: 3.334 ± 0.45
3.468ThrGlu: 3.468 ± 0.547
3.067ThrPhe: 3.067 ± 0.376
3.868ThrGly: 3.868 ± 0.566
0.867ThrHis: 0.867 ± 0.255
5.268ThrIle: 5.268 ± 0.497
3.934ThrLys: 3.934 ± 0.658
5.135ThrLeu: 5.135 ± 0.571
1.2ThrMet: 1.2 ± 0.379
4.334ThrAsn: 4.334 ± 0.636
2.467ThrPro: 2.467 ± 0.44
1.734ThrGln: 1.734 ± 0.316
1.934ThrArg: 1.934 ± 0.409
3.268ThrSer: 3.268 ± 0.49
3.001ThrThr: 3.001 ± 0.581
4.201ThrVal: 4.201 ± 0.711
0.8ThrTrp: 0.8 ± 0.187
2.267ThrTyr: 2.267 ± 0.429
0.0ThrXaa: 0.0 ± 0.0
Val
2.934ValAla: 2.934 ± 0.664
0.8ValCys: 0.8 ± 0.246
3.668ValAsp: 3.668 ± 0.355
3.934ValGlu: 3.934 ± 0.443
2.601ValPhe: 2.601 ± 0.407
2.267ValGly: 2.267 ± 0.378
0.8ValHis: 0.8 ± 0.243
5.001ValIle: 5.001 ± 0.697
5.135ValLys: 5.135 ± 0.623
4.868ValLeu: 4.868 ± 0.583
1.0ValMet: 1.0 ± 0.226
5.135ValAsn: 5.135 ± 0.535
1.334ValPro: 1.334 ± 0.343
1.6ValGln: 1.6 ± 0.304
2.467ValArg: 2.467 ± 0.402
4.535ValSer: 4.535 ± 0.525
3.668ValThr: 3.668 ± 0.509
3.334ValVal: 3.334 ± 0.39
0.467ValTrp: 0.467 ± 0.195
3.067ValTyr: 3.067 ± 0.457
0.0ValXaa: 0.0 ± 0.0
Trp
0.8TrpAla: 0.8 ± 0.232
0.133TrpCys: 0.133 ± 0.086
0.467TrpAsp: 0.467 ± 0.161
0.533TrpGlu: 0.533 ± 0.224
0.467TrpPhe: 0.467 ± 0.161
0.8TrpGly: 0.8 ± 0.232
0.133TrpHis: 0.133 ± 0.083
1.0TrpIle: 1.0 ± 0.274
1.067TrpLys: 1.067 ± 0.234
0.734TrpLeu: 0.734 ± 0.191
0.533TrpMet: 0.533 ± 0.203
0.667TrpAsn: 0.667 ± 0.163
0.133TrpPro: 0.133 ± 0.081
0.4TrpGln: 0.4 ± 0.17
0.267TrpArg: 0.267 ± 0.12
0.734TrpSer: 0.734 ± 0.253
0.734TrpThr: 0.734 ± 0.189
0.533TrpVal: 0.533 ± 0.219
0.133TrpTrp: 0.133 ± 0.088
0.6TrpTyr: 0.6 ± 0.181
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.2TyrAla: 1.2 ± 0.271
0.667TyrCys: 0.667 ± 0.226
2.734TyrAsp: 2.734 ± 0.412
2.334TyrGlu: 2.334 ± 0.375
2.734TyrPhe: 2.734 ± 0.421
1.8TyrGly: 1.8 ± 0.392
0.6TyrHis: 0.6 ± 0.223
2.334TyrIle: 2.334 ± 0.395
4.468TyrLys: 4.468 ± 0.461
4.201TyrLeu: 4.201 ± 0.523
0.8TyrMet: 0.8 ± 0.238
3.134TyrAsn: 3.134 ± 0.553
2.001TyrPro: 2.001 ± 0.351
2.067TyrGln: 2.067 ± 0.4
1.267TyrArg: 1.267 ± 0.351
3.868TyrSer: 3.868 ± 0.569
2.534TyrThr: 2.534 ± 0.42
2.601TyrVal: 2.601 ± 0.438
0.467TyrTrp: 0.467 ± 0.18
1.734TyrTyr: 1.734 ± 0.355
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (14997 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski