Helicobacter sp. CLO-3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; unclassified Helicobacter

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1846 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B8J7C3|A0A1B8J7C3_9HELI Beta-lactamase (Fragment) OS=Helicobacter sp. CLO-3 OX=211 GN=BA723_07860 PE=3 SV=1
MM1 pKa = 7.39MSFACPAVAVTTDD14 pKa = 3.91FSKK17 pKa = 9.7STLSIFSEE25 pKa = 4.16SLEE28 pKa = 4.09DD29 pKa = 4.3LEE31 pKa = 4.67SLRR34 pKa = 11.84LVEE37 pKa = 4.33SRR39 pKa = 11.84EE40 pKa = 4.14SGAVCAGVVCAFCAFCVLCAGADD63 pKa = 3.73SRR65 pKa = 11.84EE66 pKa = 4.06SLALFASLDD75 pKa = 4.04SVVVWLWVLWLAEE88 pKa = 3.97VSLWALAEE96 pKa = 4.62SGDD99 pKa = 3.82STASDD104 pKa = 4.5FEE106 pKa = 4.65AKK108 pKa = 10.14GG109 pKa = 3.31

Molecular weight: 11.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B8J6Q2|A0A1B8J6Q2_9HELI Uncharacterized protein OS=Helicobacter sp. CLO-3 OX=211 GN=BA723_08795 PE=4 SV=1
MM1 pKa = 7.19RR2 pKa = 11.84QKK4 pKa = 10.6PKK6 pKa = 10.64AKK8 pKa = 10.09HH9 pKa = 4.37VTSKK13 pKa = 11.16AKK15 pKa = 10.62AKK17 pKa = 10.14AHH19 pKa = 5.87SRR21 pKa = 11.84LKK23 pKa = 10.53ASKK26 pKa = 10.21PSATKK31 pKa = 10.39VKK33 pKa = 10.49SIAPASKK40 pKa = 10.35LKK42 pKa = 10.59SASQARR48 pKa = 11.84QKK50 pKa = 10.95AKK52 pKa = 10.4KK53 pKa = 9.88APQKK57 pKa = 10.59SQSQRR62 pKa = 11.84QAISAVRR69 pKa = 3.36

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1846

0

1846

591729

37

2885

320.5

35.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.279 ± 0.07

1.193 ± 0.023

5.463 ± 0.045

6.043 ± 0.057

4.891 ± 0.052

6.349 ± 0.058

1.812 ± 0.026

7.686 ± 0.057

7.294 ± 0.056

9.643 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.028

4.794 ± 0.048

3.477 ± 0.033

3.618 ± 0.039

4.286 ± 0.04

8.047 ± 0.054

4.35 ± 0.041

5.285 ± 0.05

0.809 ± 0.018

3.348 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski