Amino acid dipepetide frequency for Cryobacterium psychrotolerans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.202AlaAla: 20.202 ± 0.23
0.656AlaCys: 0.656 ± 0.028
7.694AlaAsp: 7.694 ± 0.095
8.059AlaGlu: 8.059 ± 0.111
3.838AlaPhe: 3.838 ± 0.066
12.947AlaGly: 12.947 ± 0.13
2.401AlaHis: 2.401 ± 0.049
5.799AlaIle: 5.799 ± 0.083
2.807AlaLys: 2.807 ± 0.075
13.972AlaLeu: 13.972 ± 0.165
2.601AlaMet: 2.601 ± 0.057
2.593AlaAsn: 2.593 ± 0.055
6.251AlaPro: 6.251 ± 0.119
3.617AlaGln: 3.617 ± 0.067
9.192AlaArg: 9.192 ± 0.119
7.365AlaSer: 7.365 ± 0.098
7.261AlaThr: 7.261 ± 0.111
11.11AlaVal: 11.11 ± 0.136
1.794AlaTrp: 1.794 ± 0.045
2.355AlaTyr: 2.355 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.652CysAla: 0.652 ± 0.026
0.059CysCys: 0.059 ± 0.009
0.36CysAsp: 0.36 ± 0.02
0.244CysGlu: 0.244 ± 0.018
0.178CysPhe: 0.178 ± 0.014
0.61CysGly: 0.61 ± 0.028
0.116CysHis: 0.116 ± 0.011
0.208CysIle: 0.208 ± 0.013
0.071CysLys: 0.071 ± 0.008
0.429CysLeu: 0.429 ± 0.023
0.096CysMet: 0.096 ± 0.008
0.111CysAsn: 0.111 ± 0.013
0.327CysPro: 0.327 ± 0.019
0.126CysGln: 0.126 ± 0.012
0.346CysArg: 0.346 ± 0.018
0.356CysSer: 0.356 ± 0.022
0.367CysThr: 0.367 ± 0.027
0.47CysVal: 0.47 ± 0.023
0.082CysTrp: 0.082 ± 0.009
0.124CysTyr: 0.124 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
8.08AspAla: 8.08 ± 0.103
0.273AspCys: 0.273 ± 0.018
3.348AspAsp: 3.348 ± 0.068
3.813AspGlu: 3.813 ± 0.074
1.88AspPhe: 1.88 ± 0.043
5.283AspGly: 5.283 ± 0.086
1.189AspHis: 1.189 ± 0.036
2.561AspIle: 2.561 ± 0.054
1.231AspLys: 1.231 ± 0.041
6.118AspLeu: 6.118 ± 0.086
0.833AspMet: 0.833 ± 0.029
1.144AspAsn: 1.144 ± 0.042
3.726AspPro: 3.726 ± 0.058
1.555AspGln: 1.555 ± 0.038
4.136AspArg: 4.136 ± 0.073
2.89AspSer: 2.89 ± 0.058
3.067AspThr: 3.067 ± 0.063
4.992AspVal: 4.992 ± 0.08
0.945AspTrp: 0.945 ± 0.035
1.357AspTyr: 1.357 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
6.887GluAla: 6.887 ± 0.102
0.24GluCys: 0.24 ± 0.016
2.512GluAsp: 2.512 ± 0.055
2.721GluGlu: 2.721 ± 0.058
1.838GluPhe: 1.838 ± 0.042
3.74GluGly: 3.74 ± 0.074
1.415GluHis: 1.415 ± 0.046
2.803GluIle: 2.803 ± 0.059
1.513GluLys: 1.513 ± 0.05
6.779GluLeu: 6.779 ± 0.101
0.982GluMet: 0.982 ± 0.037
1.326GluAsn: 1.326 ± 0.038
2.98GluPro: 2.98 ± 0.059
2.086GluGln: 2.086 ± 0.057
4.522GluArg: 4.522 ± 0.082
3.121GluSer: 3.121 ± 0.057
3.054GluThr: 3.054 ± 0.054
4.202GluVal: 4.202 ± 0.077
0.758GluTrp: 0.758 ± 0.027
1.094GluTyr: 1.094 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
4.058PheAla: 4.058 ± 0.068
0.192PheCys: 0.192 ± 0.014
2.32PheAsp: 2.32 ± 0.049
1.826PheGlu: 1.826 ± 0.043
1.087PhePhe: 1.087 ± 0.037
3.491PheGly: 3.491 ± 0.069
0.597PheHis: 0.597 ± 0.025
1.314PheIle: 1.314 ± 0.04
0.586PheLys: 0.586 ± 0.027
3.003PheLeu: 3.003 ± 0.066
0.504PheMet: 0.504 ± 0.023
0.788PheAsn: 0.788 ± 0.029
1.484PhePro: 1.484 ± 0.044
0.783PheGln: 0.783 ± 0.029
1.82PheArg: 1.82 ± 0.045
1.883PheSer: 1.883 ± 0.048
2.146PheThr: 2.146 ± 0.048
2.757PheVal: 2.757 ± 0.059
0.452PheTrp: 0.452 ± 0.025
0.698PheTyr: 0.698 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
10.564GlyAla: 10.564 ± 0.118
0.614GlyCys: 0.614 ± 0.027
4.773GlyAsp: 4.773 ± 0.075
4.717GlyGlu: 4.717 ± 0.087
3.258GlyPhe: 3.258 ± 0.062
7.662GlyGly: 7.662 ± 0.121
1.929GlyHis: 1.929 ± 0.049
4.968GlyIle: 4.968 ± 0.08
2.495GlyLys: 2.495 ± 0.05
9.457GlyLeu: 9.457 ± 0.105
1.968GlyMet: 1.968 ± 0.045
1.891GlyAsn: 1.891 ± 0.054
3.997GlyPro: 3.997 ± 0.072
2.739GlyGln: 2.739 ± 0.054
6.327GlyArg: 6.327 ± 0.093
5.846GlySer: 5.846 ± 0.086
5.876GlyThr: 5.876 ± 0.092
7.542GlyVal: 7.542 ± 0.09
1.562GlyTrp: 1.562 ± 0.047
2.339GlyTyr: 2.339 ± 0.053
0.001GlyXaa: 0.001 ± 0.001
His
2.255HisAla: 2.255 ± 0.052
0.13HisCys: 0.13 ± 0.011
1.249HisAsp: 1.249 ± 0.036
1.158HisGlu: 1.158 ± 0.034
0.589HisPhe: 0.589 ± 0.024
2.002HisGly: 2.002 ± 0.051
0.54HisHis: 0.54 ± 0.025
0.787HisIle: 0.787 ± 0.026
0.328HisLys: 0.328 ± 0.017
2.019HisLeu: 2.019 ± 0.052
0.32HisMet: 0.32 ± 0.018
0.43HisAsn: 0.43 ± 0.023
1.492HisPro: 1.492 ± 0.044
0.561HisGln: 0.561 ± 0.025
1.485HisArg: 1.485 ± 0.04
1.134HisSer: 1.134 ± 0.034
1.056HisThr: 1.056 ± 0.031
1.578HisVal: 1.578 ± 0.042
0.271HisTrp: 0.271 ± 0.017
0.468HisTyr: 0.468 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.593IleAla: 6.593 ± 0.093
0.262IleCys: 0.262 ± 0.017
3.461IleAsp: 3.461 ± 0.063
2.835IleGlu: 2.835 ± 0.056
1.31IlePhe: 1.31 ± 0.041
4.743IleGly: 4.743 ± 0.077
0.795IleHis: 0.795 ± 0.029
2.089IleIle: 2.089 ± 0.06
0.992IleLys: 0.992 ± 0.038
4.195IleLeu: 4.195 ± 0.073
0.654IleMet: 0.654 ± 0.026
1.152IleAsn: 1.152 ± 0.032
2.625IlePro: 2.625 ± 0.053
1.057IleGln: 1.057 ± 0.034
2.995IleArg: 2.995 ± 0.05
2.594IleSer: 2.594 ± 0.057
2.905IleThr: 2.905 ± 0.065
4.624IleVal: 4.624 ± 0.071
0.528IleTrp: 0.528 ± 0.024
0.826IleTyr: 0.826 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
2.86LysAla: 2.86 ± 0.06
0.079LysCys: 0.079 ± 0.009
1.166LysAsp: 1.166 ± 0.04
1.051LysGlu: 1.051 ± 0.038
0.608LysPhe: 0.608 ± 0.027
1.665LysGly: 1.665 ± 0.049
0.513LysHis: 0.513 ± 0.023
1.137LysIle: 1.137 ± 0.037
0.909LysLys: 0.909 ± 0.04
2.233LysLeu: 2.233 ± 0.05
0.503LysMet: 0.503 ± 0.023
0.647LysAsn: 0.647 ± 0.03
1.446LysPro: 1.446 ± 0.042
0.757LysGln: 0.757 ± 0.028
1.721LysArg: 1.721 ± 0.043
1.44LysSer: 1.44 ± 0.042
1.499LysThr: 1.499 ± 0.041
1.861LysVal: 1.861 ± 0.05
0.274LysTrp: 0.274 ± 0.017
0.502LysTyr: 0.502 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
15.466LeuAla: 15.466 ± 0.17
0.578LeuCys: 0.578 ± 0.024
6.391LeuAsp: 6.391 ± 0.08
5.309LeuGlu: 5.309 ± 0.086
2.935LeuPhe: 2.935 ± 0.068
9.716LeuGly: 9.716 ± 0.107
1.941LeuHis: 1.941 ± 0.043
4.826LeuIle: 4.826 ± 0.091
2.134LeuLys: 2.134 ± 0.053
10.705LeuLeu: 10.705 ± 0.155
1.763LeuMet: 1.763 ± 0.05
2.161LeuAsn: 2.161 ± 0.044
5.729LeuPro: 5.729 ± 0.083
2.473LeuGln: 2.473 ± 0.051
7.262LeuArg: 7.262 ± 0.111
6.051LeuSer: 6.051 ± 0.08
6.555LeuThr: 6.555 ± 0.089
9.402LeuVal: 9.402 ± 0.123
1.207LeuTrp: 1.207 ± 0.039
1.779LeuTyr: 1.779 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
2.228MetAla: 2.228 ± 0.045
0.093MetCys: 0.093 ± 0.01
0.855MetAsp: 0.855 ± 0.03
0.73MetGlu: 0.73 ± 0.027
0.568MetPhe: 0.568 ± 0.024
1.336MetGly: 1.336 ± 0.039
0.323MetHis: 0.323 ± 0.019
0.931MetIle: 0.931 ± 0.035
0.52MetLys: 0.52 ± 0.022
2.029MetLeu: 2.029 ± 0.039
0.322MetMet: 0.322 ± 0.019
0.578MetAsn: 0.578 ± 0.023
1.069MetPro: 1.069 ± 0.035
0.562MetGln: 0.562 ± 0.023
1.272MetArg: 1.272 ± 0.035
1.45MetSer: 1.45 ± 0.041
1.701MetThr: 1.701 ± 0.042
1.388MetVal: 1.388 ± 0.038
0.2MetTrp: 0.2 ± 0.015
0.295MetTyr: 0.295 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.647AsnAla: 2.647 ± 0.053
0.134AsnCys: 0.134 ± 0.012
1.241AsnAsp: 1.241 ± 0.039
1.116AsnGlu: 1.116 ± 0.038
0.717AsnPhe: 0.717 ± 0.031
2.059AsnGly: 2.059 ± 0.054
0.419AsnHis: 0.419 ± 0.022
0.948AsnIle: 0.948 ± 0.03
0.558AsnLys: 0.558 ± 0.025
2.305AsnLeu: 2.305 ± 0.044
0.392AsnMet: 0.392 ± 0.019
0.553AsnAsn: 0.553 ± 0.026
1.72AsnPro: 1.72 ± 0.041
0.674AsnGln: 0.674 ± 0.027
1.536AsnArg: 1.536 ± 0.042
1.206AsnSer: 1.206 ± 0.041
1.324AsnThr: 1.324 ± 0.048
1.746AsnVal: 1.746 ± 0.042
0.342AsnTrp: 0.342 ± 0.02
0.552AsnTyr: 0.552 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
7.582ProAla: 7.582 ± 0.112
0.21ProCys: 0.21 ± 0.016
3.712ProAsp: 3.712 ± 0.064
3.68ProGlu: 3.68 ± 0.067
1.648ProPhe: 1.648 ± 0.039
5.575ProGly: 5.575 ± 0.083
1.037ProHis: 1.037 ± 0.033
2.238ProIle: 2.238 ± 0.05
1.149ProLys: 1.149 ± 0.036
4.878ProLeu: 4.878 ± 0.074
0.914ProMet: 0.914 ± 0.035
1.178ProAsn: 1.178 ± 0.034
2.292ProPro: 2.292 ± 0.064
1.432ProGln: 1.432 ± 0.039
3.203ProArg: 3.203 ± 0.065
3.109ProSer: 3.109 ± 0.063
3.299ProThr: 3.299 ± 0.066
4.94ProVal: 4.94 ± 0.08
0.781ProTrp: 0.781 ± 0.032
0.967ProTyr: 0.967 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.818GlnAla: 3.818 ± 0.066
0.129GlnCys: 0.129 ± 0.013
1.357GlnAsp: 1.357 ± 0.036
1.208GlnGlu: 1.208 ± 0.041
0.87GlnPhe: 0.87 ± 0.031
2.237GlnGly: 2.237 ± 0.05
0.568GlnHis: 0.568 ± 0.025
1.369GlnIle: 1.369 ± 0.038
0.743GlnLys: 0.743 ± 0.031
3.179GlnLeu: 3.179 ± 0.053
0.51GlnMet: 0.51 ± 0.022
0.668GlnAsn: 0.668 ± 0.028
1.501GlnPro: 1.501 ± 0.042
0.99GlnGln: 0.99 ± 0.03
2.147GlnArg: 2.147 ± 0.06
1.56GlnSer: 1.56 ± 0.047
1.542GlnThr: 1.542 ± 0.041
2.576GlnVal: 2.576 ± 0.055
0.428GlnTrp: 0.428 ± 0.023
0.623GlnTyr: 0.623 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
8.358ArgAla: 8.358 ± 0.106
0.312ArgCys: 0.312 ± 0.019
3.997ArgAsp: 3.997 ± 0.062
4.001ArgGlu: 4.001 ± 0.076
2.37ArgPhe: 2.37 ± 0.052
5.285ArgGly: 5.285 ± 0.078
1.555ArgHis: 1.555 ± 0.044
3.583ArgIle: 3.583 ± 0.064
1.661ArgLys: 1.661 ± 0.042
7.524ArgLeu: 7.524 ± 0.116
1.72ArgMet: 1.72 ± 0.041
1.521ArgAsn: 1.521 ± 0.044
3.727ArgPro: 3.727 ± 0.069
2.221ArgGln: 2.221 ± 0.047
5.705ArgArg: 5.705 ± 0.094
4.109ArgSer: 4.109 ± 0.068
3.998ArgThr: 3.998 ± 0.07
5.626ArgVal: 5.626 ± 0.087
1.071ArgTrp: 1.071 ± 0.033
1.517ArgTyr: 1.517 ± 0.044
0.001ArgXaa: 0.001 ± 0.001
Ser
7.515SerAla: 7.515 ± 0.101
0.318SerCys: 0.318 ± 0.018
3.067SerAsp: 3.067 ± 0.053
2.811SerGlu: 2.811 ± 0.056
2.044SerPhe: 2.044 ± 0.051
6.185SerGly: 6.185 ± 0.098
1.106SerHis: 1.106 ± 0.036
2.782SerIle: 2.782 ± 0.054
1.27SerLys: 1.27 ± 0.038
5.877SerLeu: 5.877 ± 0.088
1.276SerMet: 1.276 ± 0.037
1.22SerAsn: 1.22 ± 0.033
3.189SerPro: 3.189 ± 0.054
1.495SerGln: 1.495 ± 0.039
4.017SerArg: 4.017 ± 0.071
3.533SerSer: 3.533 ± 0.072
3.654SerThr: 3.654 ± 0.062
5.074SerVal: 5.074 ± 0.075
0.905SerTrp: 0.905 ± 0.032
1.227SerTyr: 1.227 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
7.632ThrAla: 7.632 ± 0.112
0.315ThrCys: 0.315 ± 0.019
3.675ThrAsp: 3.675 ± 0.073
3.191ThrGlu: 3.191 ± 0.055
1.924ThrPhe: 1.924 ± 0.045
6.084ThrGly: 6.084 ± 0.085
1.125ThrHis: 1.125 ± 0.032
2.984ThrIle: 2.984 ± 0.06
1.266ThrLys: 1.266 ± 0.04
6.132ThrLeu: 6.132 ± 0.085
1.032ThrMet: 1.032 ± 0.035
1.322ThrAsn: 1.322 ± 0.04
3.816ThrPro: 3.816 ± 0.071
1.481ThrGln: 1.481 ± 0.042
3.784ThrArg: 3.784 ± 0.057
3.486ThrSer: 3.486 ± 0.072
3.678ThrThr: 3.678 ± 0.083
5.842ThrVal: 5.842 ± 0.099
0.839ThrTrp: 0.839 ± 0.03
1.14ThrTyr: 1.14 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
11.151ValAla: 11.151 ± 0.121
0.516ValCys: 0.516 ± 0.024
5.227ValAsp: 5.227 ± 0.074
4.439ValGlu: 4.439 ± 0.078
2.882ValPhe: 2.882 ± 0.052
7.043ValGly: 7.043 ± 0.095
1.689ValHis: 1.689 ± 0.042
4.549ValIle: 4.549 ± 0.082
1.807ValLys: 1.807 ± 0.046
9.295ValLeu: 9.295 ± 0.135
1.461ValMet: 1.461 ± 0.041
2.016ValAsn: 2.016 ± 0.047
4.616ValPro: 4.616 ± 0.074
2.237ValGln: 2.237 ± 0.056
5.678ValArg: 5.678 ± 0.072
5.258ValSer: 5.258 ± 0.078
5.824ValThr: 5.824 ± 0.089
8.138ValVal: 8.138 ± 0.104
1.034ValTrp: 1.034 ± 0.036
1.593ValTyr: 1.593 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
1.602TrpAla: 1.602 ± 0.043
0.086TrpCys: 0.086 ± 0.009
0.727TrpAsp: 0.727 ± 0.029
0.552TrpGlu: 0.552 ± 0.025
0.516TrpPhe: 0.516 ± 0.023
0.952TrpGly: 0.952 ± 0.036
0.3TrpHis: 0.3 ± 0.017
0.646TrpIle: 0.646 ± 0.025
0.391TrpLys: 0.391 ± 0.024
1.863TrpLeu: 1.863 ± 0.05
0.328TrpMet: 0.328 ± 0.02
0.418TrpAsn: 0.418 ± 0.023
0.735TrpPro: 0.735 ± 0.027
0.57TrpGln: 0.57 ± 0.025
1.082TrpArg: 1.082 ± 0.035
0.877TrpSer: 0.877 ± 0.028
0.825TrpThr: 0.825 ± 0.031
1.011TrpVal: 1.011 ± 0.039
0.336TrpTrp: 0.336 ± 0.018
0.317TrpTyr: 0.317 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.366TyrAla: 2.366 ± 0.052
0.136TyrCys: 0.136 ± 0.012
1.289TyrAsp: 1.289 ± 0.045
1.109TyrGlu: 1.109 ± 0.036
0.729TyrPhe: 0.729 ± 0.027
1.91TyrGly: 1.91 ± 0.045
0.295TyrHis: 0.295 ± 0.016
0.713TyrIle: 0.713 ± 0.032
0.441TyrLys: 0.441 ± 0.022
2.391TyrLeu: 2.391 ± 0.05
0.273TyrMet: 0.273 ± 0.016
0.471TyrAsn: 0.471 ± 0.019
1.1TyrPro: 1.1 ± 0.032
0.608TyrGln: 0.608 ± 0.025
1.636TyrArg: 1.636 ± 0.046
1.294TyrSer: 1.294 ± 0.036
1.115TyrThr: 1.115 ± 0.037
1.572TyrVal: 1.572 ± 0.04
0.324TyrTrp: 0.324 ± 0.02
0.475TyrTyr: 0.475 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.001XaaSer: 0.001 ± 0.001
0.0XaaThr: 0.0 ± 0.0
0.001XaaVal: 0.001 ± 0.001
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.004XaaXaa: 0.004 ± 0.003
Statistics based on 3047 proteins (978152 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski