Tobacco curly shoot virus - [Y41]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tobacco curly shoot virus

Average proteome isoelectric point is 8.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8QL10|Q8QL10_9GEMI Transcriptional activator protein OS=Tobacco curly shoot virus - [Y41] OX=223250 GN=AC2 PE=3 SV=1
MM1 pKa = 7.7APPNKK6 pKa = 9.62FRR8 pKa = 11.84INAKK12 pKa = 10.24NYY14 pKa = 8.35FLTYY18 pKa = 7.85PHH20 pKa = 7.15CSLTKK25 pKa = 10.57EE26 pKa = 4.11EE27 pKa = 4.79ALSQIKK33 pKa = 10.39ALEE36 pKa = 4.09TPINKK41 pKa = 9.26LFIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.5LHH51 pKa = 6.58EE52 pKa = 6.12DD53 pKa = 3.79GTPHH57 pKa = 6.72LHH59 pKa = 6.66VLIQFEE65 pKa = 5.54GKK67 pKa = 9.21FQCKK71 pKa = 7.13NQRR74 pKa = 11.84FFDD77 pKa = 3.82LTSPTRR83 pKa = 11.84SAHH86 pKa = 4.94FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.78SSTDD99 pKa = 2.72VKK101 pKa = 10.56TYY103 pKa = 8.5MEE105 pKa = 4.87KK106 pKa = 10.85DD107 pKa = 3.31GDD109 pKa = 4.13VLDD112 pKa = 4.83HH113 pKa = 7.41GIFQIDD119 pKa = 3.3GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.26AYY134 pKa = 10.9AEE136 pKa = 4.72AINSGSKK143 pKa = 10.61ASALNILRR151 pKa = 11.84EE152 pKa = 4.05KK153 pKa = 10.62APKK156 pKa = 10.5DD157 pKa = 3.42FVLQFHH163 pKa = 6.68NLNSNLDD170 pKa = 4.09RR171 pKa = 11.84IFTPPIEE178 pKa = 4.5EE179 pKa = 4.42YY180 pKa = 10.25ISPFLSSSFDD190 pKa = 3.53QVPEE194 pKa = 4.29EE195 pKa = 3.97LDD197 pKa = 2.79EE198 pKa = 4.17WAAEE202 pKa = 4.2NVVSAAARR210 pKa = 11.84PLRR213 pKa = 11.84PMSIVIEE220 pKa = 4.37GDD222 pKa = 3.13SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.39LSPKK249 pKa = 10.12VYY251 pKa = 11.11NNDD254 pKa = 2.08AWYY257 pKa = 10.88NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.34KK312 pKa = 10.62EE313 pKa = 3.85FLDD316 pKa = 3.97EE317 pKa = 4.83EE318 pKa = 4.8KK319 pKa = 10.25NTALKK324 pKa = 10.55NWALKK329 pKa = 10.24NAIFVTLEE337 pKa = 3.74SPLYY341 pKa = 10.27SGSNQSEE348 pKa = 3.93AQASQEE354 pKa = 4.11GEE356 pKa = 4.17QTSSCC361 pKa = 4.0

Molecular weight: 40.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8UYN4|Q8UYN4_9GEMI Protein V2 OS=Tobacco curly shoot virus - [Y41] OX=223250 GN=AV2 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.79VSRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 10.52ARR42 pKa = 11.84AWANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.87PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.1SRR80 pKa = 11.84HH81 pKa = 6.13DD82 pKa = 3.26IQHH85 pKa = 6.35IGKK88 pKa = 8.97VMCVSDD94 pKa = 3.59VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.38NVHH172 pKa = 6.5RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 8.56QVLRR180 pKa = 11.84KK181 pKa = 8.48WHH183 pKa = 5.71ATVAGGQYY191 pKa = 10.7ASKK194 pKa = 10.19EE195 pKa = 3.72QAFVKK200 pKa = 10.57KK201 pKa = 9.6FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.08YY219 pKa = 8.01EE220 pKa = 4.07NHH222 pKa = 6.29SEE224 pKa = 4.11NALMLYY230 pKa = 7.58MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1099

99

361

183.2

21.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.914 ± 0.917

2.184 ± 0.448

4.823 ± 0.392

5.187 ± 0.742

4.368 ± 0.43

4.459 ± 0.488

4.186 ± 0.779

5.551 ± 1.359

6.642 ± 0.439

6.824 ± 1.146

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.73 ± 0.747

5.551 ± 0.689

5.641 ± 0.618

4.823 ± 0.692

6.46 ± 1.2

8.644 ± 1.487

4.823 ± 0.435

6.096 ± 1.563

1.365 ± 0.161

3.731 ± 0.687
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski