Staphylococcus phage IME1367_01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JPW5|A0A1W6JPW5_9CAUD Portal protein OS=Staphylococcus phage IME1367_01 OX=1965491 PE=4 SV=1
MM1 pKa = 7.8EE2 pKa = 4.07YY3 pKa = 10.26QVRR6 pKa = 11.84INFKK10 pKa = 8.96ITGHH14 pKa = 5.86VNTFIPVGKK23 pKa = 10.64YY24 pKa = 7.15EE25 pKa = 4.08TLEE28 pKa = 4.24DD29 pKa = 3.68RR30 pKa = 11.84LQEE33 pKa = 4.44KK34 pKa = 10.41INEE37 pKa = 4.0LRR39 pKa = 11.84EE40 pKa = 4.19CPNDD44 pKa = 3.43ILEE47 pKa = 4.56LDD49 pKa = 3.48ITIDD53 pKa = 3.66DD54 pKa = 4.7AEE56 pKa = 4.4VEE58 pKa = 4.26

Molecular weight: 6.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JPR6|A0A1W6JPR6_9CAUD Uncharacterized protein OS=Staphylococcus phage IME1367_01 OX=1965491 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 10.17IINYY6 pKa = 7.21QVKK9 pKa = 9.73LRR11 pKa = 11.84KK12 pKa = 9.01IYY14 pKa = 9.14YY15 pKa = 6.93TVRR18 pKa = 11.84TNRR21 pKa = 11.84GNVFRR26 pKa = 11.84KK27 pKa = 9.56SRR29 pKa = 11.84PKK31 pKa = 10.15KK32 pKa = 9.29EE33 pKa = 4.59SIIKK37 pKa = 8.49TRR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 7.13LQKK44 pKa = 8.7YY45 pKa = 9.37ARR47 pKa = 11.84EE48 pKa = 3.7IDD50 pKa = 3.88EE51 pKa = 4.25KK52 pKa = 11.16RR53 pKa = 11.84KK54 pKa = 10.18

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12972

41

1865

190.8

21.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.419 ± 0.572

0.632 ± 0.137

6.298 ± 0.605

6.93 ± 0.538

4.016 ± 0.199

6.059 ± 0.683

1.866 ± 0.136

7.069 ± 0.23

9.15 ± 0.326

7.855 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.559 ± 0.152

7.231 ± 0.315

2.582 ± 0.174

4.093 ± 0.255

4.394 ± 0.215

5.998 ± 0.341

6.121 ± 0.281

5.99 ± 0.203

1.364 ± 0.228

4.371 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski