Amino acid dipepetide frequency for Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.378AlaAla: 12.378 ± 0.241	1.287AlaCys: 1.287 ± 0.049	7.372AlaAsp: 7.372 ± 0.136	6.224AlaGlu: 6.224 ± 0.113	3.639AlaPhe: 3.639 ± 0.08	8.619AlaGly: 8.619 ± 0.159	2.099AlaHis: 2.099 ± 0.056	5.832AlaIle: 5.832 ± 0.099	5.167AlaLys: 5.167 ± 0.093	9.558AlaLeu: 9.558 ± 0.151	3.194AlaMet: 3.194 ± 0.07	3.267AlaAsn: 3.267 ± 0.075	3.722AlaPro: 3.722 ± 0.096	4.307AlaGln: 4.307 ± 0.091	5.644AlaArg: 5.644 ± 0.122	6.279AlaSer: 6.279 ± 0.118	5.709AlaThr: 5.709 ± 0.115	8.379AlaVal: 8.379 ± 0.136	1.398AlaTrp: 1.398 ± 0.055	2.62AlaTyr: 2.62 ± 0.075	0.0AlaXaa: 0.0 ± 0.0
Cys	1.222CysAla: 1.222 ± 0.045	0.195CysCys: 0.195 ± 0.02	0.65CysAsp: 0.65 ± 0.039	0.505CysGlu: 0.505 ± 0.029	0.357CysPhe: 0.357 ± 0.025	1.14CysGly: 1.14 ± 0.046	0.242CysHis: 0.242 ± 0.022	0.5CysIle: 0.5 ± 0.029	0.332CysLys: 0.332 ± 0.023	0.853CysLeu: 0.853 ± 0.038	0.308CysMet: 0.308 ± 0.023	0.282CysAsn: 0.282 ± 0.023	0.48CysPro: 0.48 ± 0.03	0.253CysGln: 0.253 ± 0.019	0.577CysArg: 0.577 ± 0.033	0.647CysSer: 0.647 ± 0.031	0.518CysThr: 0.518 ± 0.032	0.862CysVal: 0.862 ± 0.042	0.14CysTrp: 0.14 ± 0.014	0.273CysTyr: 0.273 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	7.047AspAla: 7.047 ± 0.143	0.597AspCys: 0.597 ± 0.034	4.754AspAsp: 4.754 ± 0.124	5.03AspGlu: 5.03 ± 0.115	2.452AspPhe: 2.452 ± 0.068	6.049AspGly: 6.049 ± 0.115	1.412AspHis: 1.412 ± 0.052	3.277AspIle: 3.277 ± 0.075	2.612AspLys: 2.612 ± 0.073	5.04AspLeu: 5.04 ± 0.099	1.854AspMet: 1.854 ± 0.049	1.835AspAsn: 1.835 ± 0.057	3.272AspPro: 3.272 ± 0.07	1.785AspGln: 1.785 ± 0.055	3.39AspArg: 3.39 ± 0.101	3.68AspSer: 3.68 ± 0.089	3.165AspThr: 3.165 ± 0.083	5.186AspVal: 5.186 ± 0.114	1.007AspTrp: 1.007 ± 0.041	1.917AspTyr: 1.917 ± 0.064	0.0AspXaa: 0.0 ± 0.0
Glu	5.964GluAla: 5.964 ± 0.113	0.462GluCys: 0.462 ± 0.033	3.28GluAsp: 3.28 ± 0.077	3.352GluGlu: 3.352 ± 0.09	1.795GluPhe: 1.795 ± 0.063	3.989GluGly: 3.989 ± 0.078	1.785GluHis: 1.785 ± 0.058	2.96GluIle: 2.96 ± 0.068	2.419GluLys: 2.419 ± 0.079	5.182GluLeu: 5.182 ± 0.108	1.523GluMet: 1.523 ± 0.046	2.267GluAsn: 2.267 ± 0.063	2.647GluPro: 2.647 ± 0.076	2.88GluGln: 2.88 ± 0.079	4.2GluArg: 4.2 ± 0.101	3.437GluSer: 3.437 ± 0.083	3.525GluThr: 3.525 ± 0.091	3.622GluVal: 3.622 ± 0.084	0.737GluTrp: 0.737 ± 0.034	1.817GluTyr: 1.817 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	3.894PheAla: 3.894 ± 0.085	0.398PheCys: 0.398 ± 0.028	2.8PheAsp: 2.8 ± 0.072	1.885PheGlu: 1.885 ± 0.059	1.312PhePhe: 1.312 ± 0.055	3.409PheGly: 3.409 ± 0.082	0.827PheHis: 0.827 ± 0.039	1.752PheIle: 1.752 ± 0.06	1.27PheLys: 1.27 ± 0.05	2.697PheLeu: 2.697 ± 0.084	0.798PheMet: 0.798 ± 0.034	1.267PheAsn: 1.267 ± 0.041	1.397PhePro: 1.397 ± 0.053	1.03PheGln: 1.03 ± 0.036	1.762PheArg: 1.762 ± 0.059	2.244PheSer: 2.244 ± 0.07	2.11PheThr: 2.11 ± 0.064	2.715PheVal: 2.715 ± 0.069	0.47PheTrp: 0.47 ± 0.028	0.898PheTyr: 0.898 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	7.102GlyAla: 7.102 ± 0.144	0.91GlyCys: 0.91 ± 0.045	4.529GlyAsp: 4.529 ± 0.087	4.655GlyGlu: 4.655 ± 0.097	3.245GlyPhe: 3.245 ± 0.082	5.862GlyGly: 5.862 ± 0.123	1.839GlyHis: 1.839 ± 0.061	4.782GlyIle: 4.782 ± 0.1	4.39GlyLys: 4.39 ± 0.098	6.921GlyLeu: 6.921 ± 0.121	2.39GlyMet: 2.39 ± 0.069	2.939GlyAsn: 2.939 ± 0.079	2.53GlyPro: 2.53 ± 0.065	2.629GlyGln: 2.629 ± 0.072	4.549GlyArg: 4.549 ± 0.101	5.05GlySer: 5.05 ± 0.105	4.995GlyThr: 4.995 ± 0.093	6.276GlyVal: 6.276 ± 0.101	1.15GlyTrp: 1.15 ± 0.044	2.589GlyTyr: 2.589 ± 0.069	0.0GlyXaa: 0.0 ± 0.0
His	2.415HisAla: 2.415 ± 0.062	0.223HisCys: 0.223 ± 0.022	1.673HisAsp: 1.673 ± 0.057	1.31HisGlu: 1.31 ± 0.048	0.688HisPhe: 0.688 ± 0.034	1.992HisGly: 1.992 ± 0.062	0.625HisHis: 0.625 ± 0.032	1.165HisIle: 1.165 ± 0.045	0.638HisLys: 0.638 ± 0.034	1.622HisLeu: 1.622 ± 0.052	0.717HisMet: 0.717 ± 0.036	0.747HisAsn: 0.747 ± 0.038	1.257HisPro: 1.257 ± 0.043	0.687HisGln: 0.687 ± 0.039	1.533HisArg: 1.533 ± 0.062	1.12HisSer: 1.12 ± 0.04	1.177HisThr: 1.177 ± 0.046	1.929HisVal: 1.929 ± 0.062	0.295HisTrp: 0.295 ± 0.021	0.672HisTyr: 0.672 ± 0.039	0.0HisXaa: 0.0 ± 0.0
Ile	6.892IleAla: 6.892 ± 0.128	0.653IleCys: 0.653 ± 0.039	4.117IleAsp: 4.117 ± 0.092	3.219IleGlu: 3.219 ± 0.094	1.615IlePhe: 1.615 ± 0.066	4.659IleGly: 4.659 ± 0.103	0.968IleHis: 0.968 ± 0.039	2.874IleIle: 2.874 ± 0.079	1.892IleLys: 1.892 ± 0.067	3.912IleLeu: 3.912 ± 0.091	1.313IleMet: 1.313 ± 0.048	1.824IleAsn: 1.824 ± 0.059	2.724IlePro: 2.724 ± 0.06	1.33IleGln: 1.33 ± 0.052	2.937IleArg: 2.937 ± 0.076	3.295IleSer: 3.295 ± 0.078	3.05IleThr: 3.05 ± 0.076	4.805IleVal: 4.805 ± 0.102	0.61IleTrp: 0.61 ± 0.032	1.175IleTyr: 1.175 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	5.047LysAla: 5.047 ± 0.106	0.202LysCys: 0.202 ± 0.019	2.654LysAsp: 2.654 ± 0.088	2.455LysGlu: 2.455 ± 0.073	1.098LysPhe: 1.098 ± 0.047	3.035LysGly: 3.035 ± 0.075	0.98LysHis: 0.98 ± 0.041	2.084LysIle: 2.084 ± 0.065	2.234LysLys: 2.234 ± 0.069	3.554LysLeu: 3.554 ± 0.083	1.032LysMet: 1.032 ± 0.039	1.688LysAsn: 1.688 ± 0.064	2.495LysPro: 2.495 ± 0.072	1.824LysGln: 1.824 ± 0.058	2.762LysArg: 2.762 ± 0.08	2.559LysSer: 2.559 ± 0.078	2.919LysThr: 2.919 ± 0.08	2.945LysVal: 2.945 ± 0.093	0.535LysTrp: 0.535 ± 0.034	1.203LysTyr: 1.203 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	9.996LeuAla: 9.996 ± 0.17	1.03LeuCys: 1.03 ± 0.053	5.952LeuAsp: 5.952 ± 0.122	4.479LeuGlu: 4.479 ± 0.098	3.135LeuPhe: 3.135 ± 0.076	6.877LeuGly: 6.877 ± 0.107	1.92LeuHis: 1.92 ± 0.056	4.589LeuIle: 4.589 ± 0.107	3.802LeuLys: 3.802 ± 0.097	7.869LeuLeu: 7.869 ± 0.155	2.227LeuMet: 2.227 ± 0.073	3.05LeuAsn: 3.05 ± 0.063	4.525LeuPro: 4.525 ± 0.087	2.489LeuGln: 2.489 ± 0.069	5.065LeuArg: 5.065 ± 0.103	5.351LeuSer: 5.351 ± 0.098	5.139LeuThr: 5.139 ± 0.095	6.457LeuVal: 6.457 ± 0.114	1.0LeuTrp: 1.0 ± 0.051	1.985LeuTyr: 1.985 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	2.944MetAla: 2.944 ± 0.073	0.315MetCys: 0.315 ± 0.023	1.482MetAsp: 1.482 ± 0.052	1.255MetGlu: 1.255 ± 0.045	0.915MetPhe: 0.915 ± 0.045	1.94MetGly: 1.94 ± 0.056	0.595MetHis: 0.595 ± 0.029	1.322MetIle: 1.322 ± 0.049	1.105MetLys: 1.105 ± 0.045	2.735MetLeu: 2.735 ± 0.08	0.747MetMet: 0.747 ± 0.039	0.968MetAsn: 0.968 ± 0.039	1.63MetPro: 1.63 ± 0.058	0.898MetGln: 0.898 ± 0.043	1.855MetArg: 1.855 ± 0.052	1.85MetSer: 1.85 ± 0.055	1.977MetThr: 1.977 ± 0.057	1.914MetVal: 1.914 ± 0.057	0.29MetTrp: 0.29 ± 0.021	0.565MetTyr: 0.565 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	3.864AsnAla: 3.864 ± 0.109	0.288AsnCys: 0.288 ± 0.023	2.16AsnAsp: 2.16 ± 0.069	1.967AsnGlu: 1.967 ± 0.069	0.963AsnPhe: 0.963 ± 0.054	3.415AsnGly: 3.415 ± 0.081	0.713AsnHis: 0.713 ± 0.036	1.785AsnIle: 1.785 ± 0.057	1.373AsnLys: 1.373 ± 0.051	2.709AsnLeu: 2.709 ± 0.065	0.922AsnMet: 0.922 ± 0.037	1.213AsnAsn: 1.213 ± 0.06	2.249AsnPro: 2.249 ± 0.058	1.132AsnGln: 1.132 ± 0.046	2.037AsnArg: 2.037 ± 0.054	1.775AsnSer: 1.775 ± 0.056	2.05AsnThr: 2.05 ± 0.061	2.789AsnVal: 2.789 ± 0.075	0.457AsnTrp: 0.457 ± 0.029	0.925AsnTyr: 0.925 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	4.537ProAla: 4.537 ± 0.102	0.42ProCys: 0.42 ± 0.029	3.29ProAsp: 3.29 ± 0.09	3.395ProGlu: 3.395 ± 0.078	1.7ProPhe: 1.7 ± 0.049	3.28ProGly: 3.28 ± 0.076	0.982ProHis: 0.982 ± 0.041	2.377ProIle: 2.377 ± 0.064	2.084ProLys: 2.084 ± 0.063	3.712ProLeu: 3.712 ± 0.086	1.12ProMet: 1.12 ± 0.047	1.774ProAsn: 1.774 ± 0.049	1.275ProPro: 1.275 ± 0.049	1.854ProGln: 1.854 ± 0.066	2.177ProArg: 2.177 ± 0.064	2.97ProSer: 2.97 ± 0.075	2.765ProThr: 2.765 ± 0.075	3.729ProVal: 3.729 ± 0.085	0.68ProTrp: 0.68 ± 0.035	1.372ProTyr: 1.372 ± 0.049	0.0ProXaa: 0.0 ± 0.0
Gln	3.697GlnAla: 3.697 ± 0.095	0.315GlnCys: 0.315 ± 0.026	1.61GlnAsp: 1.61 ± 0.056	1.76GlnGlu: 1.76 ± 0.059	1.04GlnPhe: 1.04 ± 0.045	2.36GlnGly: 2.36 ± 0.067	0.828GlnHis: 0.828 ± 0.042	1.999GlnIle: 1.999 ± 0.067	1.3GlnLys: 1.3 ± 0.054	3.369GlnLeu: 3.369 ± 0.094	0.99GlnMet: 0.99 ± 0.042	1.23GlnAsn: 1.23 ± 0.053	1.779GlnPro: 1.779 ± 0.076	1.717GlnGln: 1.717 ± 0.078	2.37GlnArg: 2.37 ± 0.07	2.292GlnSer: 2.292 ± 0.075	2.052GlnThr: 2.052 ± 0.064	2.517GlnVal: 2.517 ± 0.064	0.587GlnTrp: 0.587 ± 0.032	1.112GlnTyr: 1.112 ± 0.048	0.0GlnXaa: 0.0 ± 0.0
Arg	5.139ArgAla: 5.139 ± 0.117	0.593ArgCys: 0.593 ± 0.043	3.39ArgAsp: 3.39 ± 0.092	3.607ArgGlu: 3.607 ± 0.079	2.332ArgPhe: 2.332 ± 0.067	3.694ArgGly: 3.694 ± 0.087	1.647ArgHis: 1.647 ± 0.059	3.582ArgIle: 3.582 ± 0.089	2.904ArgLys: 2.904 ± 0.081	5.316ArgLeu: 5.316 ± 0.122	1.86ArgMet: 1.86 ± 0.062	2.194ArgAsn: 2.194 ± 0.062	2.497ArgPro: 2.497 ± 0.077	2.224ArgGln: 2.224 ± 0.067	4.667ArgArg: 4.667 ± 0.173	3.18ArgSer: 3.18 ± 0.078	3.245ArgThr: 3.245 ± 0.081	4.192ArgVal: 4.192 ± 0.102	0.822ArgTrp: 0.822 ± 0.037	1.809ArgTyr: 1.809 ± 0.058	0.0ArgXaa: 0.0 ± 0.0
Ser	6.256SerAla: 6.256 ± 0.109	0.523SerCys: 0.523 ± 0.031	4.035SerAsp: 4.035 ± 0.106	3.21SerGlu: 3.21 ± 0.078	2.19SerPhe: 2.19 ± 0.068	5.336SerGly: 5.336 ± 0.12	1.338SerHis: 1.338 ± 0.046	3.235SerIle: 3.235 ± 0.072	2.534SerLys: 2.534 ± 0.07	5.349SerLeu: 5.349 ± 0.099	1.807SerMet: 1.807 ± 0.054	2.099SerAsn: 2.099 ± 0.064	2.38SerPro: 2.38 ± 0.067	2.292SerGln: 2.292 ± 0.071	3.322SerArg: 3.322 ± 0.086	3.939SerSer: 3.939 ± 0.117	3.55SerThr: 3.55 ± 0.087	4.559SerVal: 4.559 ± 0.091	0.898SerTrp: 0.898 ± 0.04	1.718SerTyr: 1.718 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	5.982ThrAla: 5.982 ± 0.119	0.55ThrCys: 0.55 ± 0.034	3.729ThrAsp: 3.729 ± 0.083	2.882ThrGlu: 2.882 ± 0.071	2.09ThrPhe: 2.09 ± 0.068	4.752ThrGly: 4.752 ± 0.079	1.257ThrHis: 1.257 ± 0.047	3.43ThrIle: 3.43 ± 0.078	2.37ThrLys: 2.37 ± 0.077	5.452ThrLeu: 5.452 ± 0.095	1.515ThrMet: 1.515 ± 0.048	2.009ThrAsn: 2.009 ± 0.062	2.997ThrPro: 2.997 ± 0.074	1.93ThrGln: 1.93 ± 0.057	2.919ThrArg: 2.919 ± 0.079	3.4ThrSer: 3.4 ± 0.083	3.532ThrThr: 3.532 ± 0.091	5.367ThrVal: 5.367 ± 0.129	0.76ThrTrp: 0.76 ± 0.037	1.56ThrTyr: 1.56 ± 0.055	0.0ThrXaa: 0.0 ± 0.0
Val	8.282ValAla: 8.282 ± 0.151	0.915ValCys: 0.915 ± 0.042	5.192ValAsp: 5.192 ± 0.104	4.53ValGlu: 4.53 ± 0.105	2.825ValPhe: 2.825 ± 0.066	5.366ValGly: 5.366 ± 0.114	1.522ValHis: 1.522 ± 0.048	4.382ValIle: 4.382 ± 0.097	3.279ValLys: 3.279 ± 0.088	7.227ValLeu: 7.227 ± 0.128	1.959ValMet: 1.959 ± 0.058	2.619ValAsn: 2.619 ± 0.074	3.885ValPro: 3.885 ± 0.093	2.125ValGln: 2.125 ± 0.056	4.51ValArg: 4.51 ± 0.096	5.032ValSer: 5.032 ± 0.109	4.725ValThr: 4.725 ± 0.112	6.704ValVal: 6.704 ± 0.126	0.935ValTrp: 0.935 ± 0.039	1.789ValTyr: 1.789 ± 0.056	0.0ValXaa: 0.0 ± 0.0
Trp	1.072TrpAla: 1.072 ± 0.046	0.157TrpCys: 0.157 ± 0.016	0.778TrpAsp: 0.778 ± 0.038	0.532TrpGlu: 0.532 ± 0.028	0.52TrpPhe: 0.52 ± 0.036	0.935TrpGly: 0.935 ± 0.042	0.368TrpHis: 0.368 ± 0.027	0.732TrpIle: 0.732 ± 0.039	0.623TrpLys: 0.623 ± 0.03	1.503TrpLeu: 1.503 ± 0.063	0.415TrpMet: 0.415 ± 0.026	0.647TrpAsn: 0.647 ± 0.033	0.542TrpPro: 0.542 ± 0.028	0.573TrpGln: 0.573 ± 0.03	0.932TrpArg: 0.932 ± 0.037	0.817TrpSer: 0.817 ± 0.04	0.805TrpThr: 0.805 ± 0.043	0.822TrpVal: 0.822 ± 0.041	0.275TrpTrp: 0.275 ± 0.021	0.417TrpTyr: 0.417 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.964TyrAla: 2.964 ± 0.071	0.302TyrCys: 0.302 ± 0.025	1.912TyrAsp: 1.912 ± 0.067	1.59TyrGlu: 1.59 ± 0.051	1.022TyrPhe: 1.022 ± 0.051	2.624TyrGly: 2.624 ± 0.077	0.538TyrHis: 0.538 ± 0.027	1.207TyrIle: 1.207 ± 0.052	0.977TyrLys: 0.977 ± 0.042	2.359TyrLeu: 2.359 ± 0.066	0.61TyrMet: 0.61 ± 0.033	0.87TyrAsn: 0.87 ± 0.045	1.21TyrPro: 1.21 ± 0.047	0.965TyrGln: 0.965 ± 0.041	1.683TyrArg: 1.683 ± 0.055	1.653TyrSer: 1.653 ± 0.051	1.473TyrThr: 1.473 ± 0.058	2.039TyrVal: 2.039 ± 0.067	0.418TyrTrp: 0.418 ± 0.031	0.767TyrTyr: 0.767 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1629 proteins (599943 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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