Amino acid dipepetide frequency for Lactococcus phage 63301

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.815AlaAla: 3.815 ± 0.82
0.516AlaCys: 0.516 ± 0.197
4.021AlaAsp: 4.021 ± 0.675
4.021AlaGlu: 4.021 ± 0.75
2.268AlaPhe: 2.268 ± 0.394
4.537AlaGly: 4.537 ± 1.317
0.825AlaHis: 0.825 ± 0.306
6.083AlaIle: 6.083 ± 1.197
6.083AlaLys: 6.083 ± 0.788
5.361AlaLeu: 5.361 ± 0.882
1.65AlaMet: 1.65 ± 0.403
4.33AlaAsn: 4.33 ± 0.93
1.856AlaPro: 1.856 ± 0.43
2.578AlaGln: 2.578 ± 0.626
2.268AlaArg: 2.268 ± 0.613
3.712AlaSer: 3.712 ± 0.664
3.918AlaThr: 3.918 ± 0.655
3.815AlaVal: 3.815 ± 0.635
1.134AlaTrp: 1.134 ± 0.325
2.268AlaTyr: 2.268 ± 0.54
0.0AlaXaa: 0.0 ± 0.0
Cys
0.309CysAla: 0.309 ± 0.252
0.103CysCys: 0.103 ± 0.118
0.722CysAsp: 0.722 ± 0.297
0.206CysGlu: 0.206 ± 0.121
0.206CysPhe: 0.206 ± 0.168
0.516CysGly: 0.516 ± 0.249
0.206CysHis: 0.206 ± 0.152
0.103CysIle: 0.103 ± 0.088
0.722CysLys: 0.722 ± 0.471
0.722CysLeu: 0.722 ± 0.254
0.0CysMet: 0.0 ± 0.0
0.412CysAsn: 0.412 ± 0.222
0.0CysPro: 0.0 ± 0.0
0.309CysGln: 0.309 ± 0.181
0.619CysArg: 0.619 ± 0.445
1.031CysSer: 1.031 ± 0.338
0.206CysThr: 0.206 ± 0.167
0.103CysVal: 0.103 ± 0.104
0.0CysTrp: 0.0 ± 0.0
0.412CysTyr: 0.412 ± 0.183
0.0CysXaa: 0.0 ± 0.0
Asp
3.196AspAla: 3.196 ± 0.51
0.309AspCys: 0.309 ± 0.161
3.609AspAsp: 3.609 ± 0.601
5.258AspGlu: 5.258 ± 0.71
3.196AspPhe: 3.196 ± 0.493
4.33AspGly: 4.33 ± 0.805
0.516AspHis: 0.516 ± 0.241
4.743AspIle: 4.743 ± 0.821
4.743AspLys: 4.743 ± 0.803
5.361AspLeu: 5.361 ± 0.836
2.165AspMet: 2.165 ± 0.425
4.227AspAsn: 4.227 ± 0.502
1.856AspPro: 1.856 ± 0.496
0.619AspGln: 0.619 ± 0.26
2.578AspArg: 2.578 ± 0.464
4.021AspSer: 4.021 ± 0.499
3.093AspThr: 3.093 ± 0.621
3.609AspVal: 3.609 ± 0.751
1.65AspTrp: 1.65 ± 0.494
2.371AspTyr: 2.371 ± 0.509
0.0AspXaa: 0.0 ± 0.0
Glu
4.743GluAla: 4.743 ± 0.665
0.412GluCys: 0.412 ± 0.199
3.299GluAsp: 3.299 ± 0.813
4.949GluGlu: 4.949 ± 1.063
2.165GluPhe: 2.165 ± 0.419
2.681GluGly: 2.681 ± 0.474
1.134GluHis: 1.134 ± 0.451
6.083GluIle: 6.083 ± 0.79
7.32GluLys: 7.32 ± 1.158
6.186GluLeu: 6.186 ± 0.907
1.547GluMet: 1.547 ± 0.28
3.918GluAsn: 3.918 ± 0.742
1.65GluPro: 1.65 ± 0.404
3.815GluGln: 3.815 ± 0.666
3.712GluArg: 3.712 ± 0.733
3.402GluSer: 3.402 ± 0.584
3.196GluThr: 3.196 ± 0.573
5.052GluVal: 5.052 ± 0.816
0.722GluTrp: 0.722 ± 0.277
1.856GluTyr: 1.856 ± 0.431
0.0GluXaa: 0.0 ± 0.0
Phe
2.578PheAla: 2.578 ± 0.464
0.412PheCys: 0.412 ± 0.217
2.371PheAsp: 2.371 ± 0.422
3.093PheGlu: 3.093 ± 0.56
1.65PhePhe: 1.65 ± 0.481
3.402PheGly: 3.402 ± 0.609
0.516PheHis: 0.516 ± 0.234
2.474PheIle: 2.474 ± 0.585
3.609PheLys: 3.609 ± 0.536
2.474PheLeu: 2.474 ± 0.438
1.237PheMet: 1.237 ± 0.289
2.474PheAsn: 2.474 ± 0.742
1.443PhePro: 1.443 ± 0.349
1.134PheGln: 1.134 ± 0.328
1.134PheArg: 1.134 ± 0.304
3.196PheSer: 3.196 ± 0.803
3.402PheThr: 3.402 ± 0.661
2.062PheVal: 2.062 ± 0.418
0.412PheTrp: 0.412 ± 0.229
1.34PheTyr: 1.34 ± 0.386
0.0PheXaa: 0.0 ± 0.0
Gly
3.918GlyAla: 3.918 ± 0.933
0.0GlyCys: 0.0 ± 0.0
3.299GlyAsp: 3.299 ± 0.581
3.918GlyGlu: 3.918 ± 0.517
3.815GlyPhe: 3.815 ± 0.749
4.537GlyGly: 4.537 ± 1.067
0.722GlyHis: 0.722 ± 0.224
7.217GlyIle: 7.217 ± 1.681
5.671GlyLys: 5.671 ± 0.742
4.64GlyLeu: 4.64 ± 0.594
1.547GlyMet: 1.547 ± 0.463
3.093GlyAsn: 3.093 ± 0.626
1.134GlyPro: 1.134 ± 0.403
2.474GlyGln: 2.474 ± 0.664
2.165GlyArg: 2.165 ± 0.502
4.33GlySer: 4.33 ± 0.871
4.33GlyThr: 4.33 ± 0.757
3.299GlyVal: 3.299 ± 0.609
0.619GlyTrp: 0.619 ± 0.231
3.815GlyTyr: 3.815 ± 0.612
0.0GlyXaa: 0.0 ± 0.0
His
0.619HisAla: 0.619 ± 0.256
0.103HisCys: 0.103 ± 0.118
1.134HisAsp: 1.134 ± 0.339
0.722HisGlu: 0.722 ± 0.237
0.412HisPhe: 0.412 ± 0.166
0.516HisGly: 0.516 ± 0.268
0.206HisHis: 0.206 ± 0.139
1.031HisIle: 1.031 ± 0.383
1.031HisLys: 1.031 ± 0.313
0.722HisLeu: 0.722 ± 0.319
0.412HisMet: 0.412 ± 0.195
0.516HisAsn: 0.516 ± 0.204
0.412HisPro: 0.412 ± 0.202
0.722HisGln: 0.722 ± 0.286
0.619HisArg: 0.619 ± 0.277
0.928HisSer: 0.928 ± 0.355
0.516HisThr: 0.516 ± 0.202
0.619HisVal: 0.619 ± 0.272
0.206HisTrp: 0.206 ± 0.14
0.825HisTyr: 0.825 ± 0.28
0.0HisXaa: 0.0 ± 0.0
Ile
5.464IleAla: 5.464 ± 0.851
0.412IleCys: 0.412 ± 0.254
6.908IleAsp: 6.908 ± 0.869
4.33IleGlu: 4.33 ± 0.863
2.578IlePhe: 2.578 ± 0.49
5.155IleGly: 5.155 ± 0.689
1.031IleHis: 1.031 ± 0.304
5.258IleIle: 5.258 ± 0.852
7.32IleLys: 7.32 ± 0.718
4.846IleLeu: 4.846 ± 0.692
1.34IleMet: 1.34 ± 0.339
4.021IleAsn: 4.021 ± 0.701
2.784IlePro: 2.784 ± 0.526
3.506IleGln: 3.506 ± 0.656
2.681IleArg: 2.681 ± 0.448
7.63IleSer: 7.63 ± 1.508
4.846IleThr: 4.846 ± 0.798
4.433IleVal: 4.433 ± 0.66
0.309IleTrp: 0.309 ± 0.174
3.093IleTyr: 3.093 ± 0.646
0.0IleXaa: 0.0 ± 0.0
Lys
7.114LysAla: 7.114 ± 0.753
0.412LysCys: 0.412 ± 0.223
4.433LysAsp: 4.433 ± 0.72
7.011LysGlu: 7.011 ± 0.891
2.371LysPhe: 2.371 ± 0.498
5.774LysGly: 5.774 ± 0.661
1.443LysHis: 1.443 ± 0.521
5.877LysIle: 5.877 ± 0.835
7.527LysLys: 7.527 ± 1.187
7.939LysLeu: 7.939 ± 0.996
2.371LysMet: 2.371 ± 0.557
5.671LysAsn: 5.671 ± 0.875
2.371LysPro: 2.371 ± 0.419
4.124LysGln: 4.124 ± 0.554
4.743LysArg: 4.743 ± 0.87
5.774LysSer: 5.774 ± 0.758
5.155LysThr: 5.155 ± 0.819
4.227LysVal: 4.227 ± 0.735
1.134LysTrp: 1.134 ± 0.347
3.093LysTyr: 3.093 ± 0.668
0.0LysXaa: 0.0 ± 0.0
Leu
6.083LeuAla: 6.083 ± 0.812
0.516LeuCys: 0.516 ± 0.196
4.743LeuAsp: 4.743 ± 0.623
5.98LeuGlu: 5.98 ± 0.951
4.021LeuPhe: 4.021 ± 0.705
4.949LeuGly: 4.949 ± 0.907
0.412LeuHis: 0.412 ± 0.185
5.464LeuIle: 5.464 ± 0.869
8.454LeuLys: 8.454 ± 1.121
5.877LeuLeu: 5.877 ± 0.915
1.959LeuMet: 1.959 ± 0.384
5.98LeuAsn: 5.98 ± 0.777
2.784LeuPro: 2.784 ± 0.407
3.402LeuGln: 3.402 ± 0.684
3.402LeuArg: 3.402 ± 0.595
6.805LeuSer: 6.805 ± 0.801
5.361LeuThr: 5.361 ± 0.703
2.578LeuVal: 2.578 ± 0.369
1.031LeuTrp: 1.031 ± 0.311
1.65LeuTyr: 1.65 ± 0.446
0.0LeuXaa: 0.0 ± 0.0
Met
1.134MetAla: 1.134 ± 0.31
0.309MetCys: 0.309 ± 0.179
0.928MetAsp: 0.928 ± 0.299
1.65MetGlu: 1.65 ± 0.419
0.206MetPhe: 0.206 ± 0.147
1.031MetGly: 1.031 ± 0.429
0.412MetHis: 0.412 ± 0.223
1.34MetIle: 1.34 ± 0.338
2.268MetLys: 2.268 ± 0.457
1.753MetLeu: 1.753 ± 0.479
0.309MetMet: 0.309 ± 0.177
1.443MetAsn: 1.443 ± 0.413
1.134MetPro: 1.134 ± 0.328
0.825MetGln: 0.825 ± 0.291
1.031MetArg: 1.031 ± 0.336
2.371MetSer: 2.371 ± 0.505
2.784MetThr: 2.784 ± 0.635
0.825MetVal: 0.825 ± 0.264
0.206MetTrp: 0.206 ± 0.128
0.928MetTyr: 0.928 ± 0.25
0.0MetXaa: 0.0 ± 0.0
Asn
3.299AsnAla: 3.299 ± 0.563
0.309AsnCys: 0.309 ± 0.165
3.093AsnAsp: 3.093 ± 0.484
3.402AsnGlu: 3.402 ± 0.609
3.196AsnPhe: 3.196 ± 0.495
4.33AsnGly: 4.33 ± 0.817
0.825AsnHis: 0.825 ± 0.343
4.846AsnIle: 4.846 ± 0.638
4.743AsnLys: 4.743 ± 0.795
4.949AsnLeu: 4.949 ± 0.764
0.516AsnMet: 0.516 ± 0.223
3.815AsnAsn: 3.815 ± 0.715
2.99AsnPro: 2.99 ± 0.49
2.99AsnGln: 2.99 ± 0.585
1.443AsnArg: 1.443 ± 0.511
4.537AsnSer: 4.537 ± 0.745
2.99AsnThr: 2.99 ± 0.531
3.918AsnVal: 3.918 ± 0.583
1.134AsnTrp: 1.134 ± 0.405
2.165AsnTyr: 2.165 ± 0.538
0.0AsnXaa: 0.0 ± 0.0
Pro
1.34ProAla: 1.34 ± 0.396
0.103ProCys: 0.103 ± 0.096
2.062ProAsp: 2.062 ± 0.465
1.856ProGlu: 1.856 ± 0.443
1.237ProPhe: 1.237 ± 0.364
0.928ProGly: 0.928 ± 0.284
0.206ProHis: 0.206 ± 0.12
3.093ProIle: 3.093 ± 0.621
3.196ProLys: 3.196 ± 0.664
3.506ProLeu: 3.506 ± 0.544
0.928ProMet: 0.928 ± 0.343
2.062ProAsn: 2.062 ± 0.499
0.722ProPro: 0.722 ± 0.326
1.134ProGln: 1.134 ± 0.259
1.134ProArg: 1.134 ± 0.349
2.371ProSer: 2.371 ± 0.42
2.165ProThr: 2.165 ± 0.463
2.371ProVal: 2.371 ± 0.444
0.206ProTrp: 0.206 ± 0.134
0.928ProTyr: 0.928 ± 0.315
0.0ProXaa: 0.0 ± 0.0
Gln
4.33GlnAla: 4.33 ± 0.891
0.103GlnCys: 0.103 ± 0.096
1.856GlnAsp: 1.856 ± 0.52
3.093GlnGlu: 3.093 ± 0.755
1.031GlnPhe: 1.031 ± 0.366
1.547GlnGly: 1.547 ± 0.418
0.206GlnHis: 0.206 ± 0.147
3.402GlnIle: 3.402 ± 0.416
4.537GlnLys: 4.537 ± 0.609
2.99GlnLeu: 2.99 ± 0.624
0.206GlnMet: 0.206 ± 0.149
1.959GlnAsn: 1.959 ± 0.514
0.825GlnPro: 0.825 ± 0.3
1.34GlnGln: 1.34 ± 0.326
2.062GlnArg: 2.062 ± 0.354
3.196GlnSer: 3.196 ± 0.707
1.856GlnThr: 1.856 ± 0.455
2.474GlnVal: 2.474 ± 0.468
0.722GlnTrp: 0.722 ± 0.259
1.856GlnTyr: 1.856 ± 0.413
0.0GlnXaa: 0.0 ± 0.0
Arg
2.578ArgAla: 2.578 ± 0.475
0.928ArgCys: 0.928 ± 0.337
2.578ArgAsp: 2.578 ± 0.495
1.753ArgGlu: 1.753 ± 0.448
2.062ArgPhe: 2.062 ± 0.463
1.34ArgGly: 1.34 ± 0.409
0.412ArgHis: 0.412 ± 0.225
3.609ArgIle: 3.609 ± 0.723
3.299ArgLys: 3.299 ± 0.627
3.609ArgLeu: 3.609 ± 0.69
0.309ArgMet: 0.309 ± 0.191
1.856ArgAsn: 1.856 ± 0.435
1.134ArgPro: 1.134 ± 0.34
1.753ArgGln: 1.753 ± 0.488
1.753ArgArg: 1.753 ± 0.547
2.578ArgSer: 2.578 ± 0.505
2.062ArgThr: 2.062 ± 0.472
2.062ArgVal: 2.062 ± 0.492
0.309ArgTrp: 0.309 ± 0.181
2.165ArgTyr: 2.165 ± 0.533
0.0ArgXaa: 0.0 ± 0.0
Ser
5.361SerAla: 5.361 ± 1.094
0.412SerCys: 0.412 ± 0.285
4.433SerAsp: 4.433 ± 0.611
4.537SerGlu: 4.537 ± 0.646
3.093SerPhe: 3.093 ± 0.556
7.527SerGly: 7.527 ± 1.523
0.928SerHis: 0.928 ± 0.316
4.949SerIle: 4.949 ± 0.812
4.537SerLys: 4.537 ± 0.835
5.774SerLeu: 5.774 ± 0.985
2.268SerMet: 2.268 ± 0.415
4.846SerAsn: 4.846 ± 0.858
2.062SerPro: 2.062 ± 0.492
2.062SerGln: 2.062 ± 0.406
2.268SerArg: 2.268 ± 0.544
4.846SerSer: 4.846 ± 1.139
4.433SerThr: 4.433 ± 0.769
3.712SerVal: 3.712 ± 0.793
0.825SerTrp: 0.825 ± 0.277
3.093SerTyr: 3.093 ± 0.585
0.0SerXaa: 0.0 ± 0.0
Thr
3.506ThrAla: 3.506 ± 0.825
0.309ThrCys: 0.309 ± 0.204
4.021ThrAsp: 4.021 ± 0.837
4.124ThrGlu: 4.124 ± 0.539
2.268ThrPhe: 2.268 ± 0.442
4.846ThrGly: 4.846 ± 0.805
0.928ThrHis: 0.928 ± 0.291
4.949ThrIle: 4.949 ± 0.794
4.537ThrLys: 4.537 ± 0.721
5.568ThrLeu: 5.568 ± 0.643
1.134ThrMet: 1.134 ± 0.34
2.474ThrAsn: 2.474 ± 0.621
2.578ThrPro: 2.578 ± 0.636
2.99ThrGln: 2.99 ± 0.49
0.722ThrArg: 0.722 ± 0.245
3.815ThrSer: 3.815 ± 0.788
4.743ThrThr: 4.743 ± 0.917
5.877ThrVal: 5.877 ± 0.744
1.134ThrTrp: 1.134 ± 0.291
2.062ThrTyr: 2.062 ± 0.588
0.0ThrXaa: 0.0 ± 0.0
Val
2.887ValAla: 2.887 ± 0.675
0.412ValCys: 0.412 ± 0.226
4.33ValAsp: 4.33 ± 0.743
4.433ValGlu: 4.433 ± 0.691
2.474ValPhe: 2.474 ± 0.45
3.299ValGly: 3.299 ± 0.618
0.619ValHis: 0.619 ± 0.267
4.124ValIle: 4.124 ± 0.718
5.155ValLys: 5.155 ± 0.749
4.743ValLeu: 4.743 ± 0.688
1.547ValMet: 1.547 ± 0.349
3.815ValAsn: 3.815 ± 0.603
2.474ValPro: 2.474 ± 0.478
1.34ValGln: 1.34 ± 0.359
1.65ValArg: 1.65 ± 0.378
3.609ValSer: 3.609 ± 0.701
4.537ValThr: 4.537 ± 0.833
4.433ValVal: 4.433 ± 0.606
0.516ValTrp: 0.516 ± 0.229
1.34ValTyr: 1.34 ± 0.333
0.0ValXaa: 0.0 ± 0.0
Trp
0.619TrpAla: 0.619 ± 0.292
0.309TrpCys: 0.309 ± 0.186
0.619TrpAsp: 0.619 ± 0.245
1.031TrpGlu: 1.031 ± 0.291
0.825TrpPhe: 0.825 ± 0.315
0.619TrpGly: 0.619 ± 0.388
0.103TrpHis: 0.103 ± 0.1
0.722TrpIle: 0.722 ± 0.255
1.134TrpLys: 1.134 ± 0.312
1.443TrpLeu: 1.443 ± 0.366
0.103TrpMet: 0.103 ± 0.126
0.928TrpAsn: 0.928 ± 0.254
0.103TrpPro: 0.103 ± 0.088
0.619TrpGln: 0.619 ± 0.199
0.619TrpArg: 0.619 ± 0.229
1.237TrpSer: 1.237 ± 0.289
0.516TrpThr: 0.516 ± 0.219
0.722TrpVal: 0.722 ± 0.264
0.206TrpTrp: 0.206 ± 0.163
0.309TrpTyr: 0.309 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.165TyrAla: 2.165 ± 0.533
0.516TyrCys: 0.516 ± 0.27
2.99TyrAsp: 2.99 ± 0.631
2.474TyrGlu: 2.474 ± 0.509
1.34TyrPhe: 1.34 ± 0.379
2.681TyrGly: 2.681 ± 0.517
0.619TyrHis: 0.619 ± 0.218
2.578TyrIle: 2.578 ± 0.469
2.681TyrLys: 2.681 ± 0.578
2.99TyrLeu: 2.99 ± 0.633
1.237TyrMet: 1.237 ± 0.283
1.65TyrAsn: 1.65 ± 0.362
1.237TyrPro: 1.237 ± 0.387
1.856TyrGln: 1.856 ± 0.365
1.547TyrArg: 1.547 ± 0.359
2.681TyrSer: 2.681 ± 0.491
2.474TyrThr: 2.474 ± 0.714
1.547TyrVal: 1.547 ± 0.303
0.309TyrTrp: 0.309 ± 0.151
1.237TyrTyr: 1.237 ± 0.342
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (9700 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski