Phycicoccus sp. HDW14

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Phycicoccus; unclassified Phycicoccus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3383 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8G104|A0A6G8G104_9MICO Very short patch repair endonuclease OS=Phycicoccus sp. HDW14 OX=2714941 GN=G7075_16510 PE=3 SV=1
MM1 pKa = 7.42SAPAGWHH8 pKa = 5.41PQPDD12 pKa = 3.52GRR14 pKa = 11.84EE15 pKa = 4.0RR16 pKa = 11.84WWDD19 pKa = 3.41GQQWTEE25 pKa = 4.01HH26 pKa = 5.71FRR28 pKa = 11.84DD29 pKa = 3.77PATAAPTPGEE39 pKa = 4.21TQVLGTTGATTYY51 pKa = 11.01GSGGGYY57 pKa = 7.84GTTEE61 pKa = 3.88GSGYY65 pKa = 8.86PAPDD69 pKa = 3.48AYY71 pKa = 10.31GAPGTPGATGYY82 pKa = 7.62GTPAGQWPPQQKK94 pKa = 10.14QGMSGTAKK102 pKa = 9.89GCLIAAVVVVVLVIAAVVVGGIFFARR128 pKa = 11.84TVSTTVDD135 pKa = 3.51QVQSAIPSNFPSNLPTNQGDD155 pKa = 3.91PVTTTVGGGFDD166 pKa = 4.02LPNISVADD174 pKa = 3.76GWTLEE179 pKa = 4.17DD180 pKa = 3.19QGGVGKK186 pKa = 8.83TIEE189 pKa = 4.19NMTGTFTGAGDD200 pKa = 3.64VPTAFSMTFTGGEE213 pKa = 3.97QTLCTASPEE222 pKa = 4.43SGSDD226 pKa = 3.22TVTVSCIPVFGDD238 pKa = 3.3VADD241 pKa = 4.46DD242 pKa = 4.41AEE244 pKa = 4.74VTVTPTFF251 pKa = 3.54

Molecular weight: 25.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8G2M4|A0A6G8G2M4_9MICO GNAT family N-acetyltransferase OS=Phycicoccus sp. HDW14 OX=2714941 GN=G7075_19805 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3383

0

3383

1002685

28

2514

296.4

31.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.634 ± 0.059

0.727 ± 0.013

6.277 ± 0.044

5.41 ± 0.053

2.519 ± 0.026

9.642 ± 0.039

2.214 ± 0.021

2.617 ± 0.037

1.692 ± 0.035

10.219 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.712 ± 0.019

1.482 ± 0.023

5.95 ± 0.036

2.35 ± 0.023

8.237 ± 0.052

5.331 ± 0.034

6.4 ± 0.054

10.279 ± 0.046

1.591 ± 0.02

1.714 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski