Clavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Metschnikowiaceae; Clavispora; Clavispora lusitaniae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5932 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4Y8G9|C4Y8G9_CLAL4 NADH-cytochrome b5 reductase OS=Clavispora lusitaniae (strain ATCC 42720) OX=306902 GN=CLUG_04497 PE=3 SV=1
MM1 pKa = 7.64EE2 pKa = 5.87PNSWWYY8 pKa = 10.76RR9 pKa = 11.84EE10 pKa = 4.03ALEE13 pKa = 4.35TMFWPAYY20 pKa = 9.88LSSLTPTTNIGASAEE35 pKa = 4.24GAVMTTFLAPASMCFWAPSSLVKK58 pKa = 10.32TPVEE62 pKa = 4.18SMTNSTPSLPHH73 pKa = 6.56GIWAEE78 pKa = 4.09SLSWWTLIFLPSMTKK93 pKa = 10.45APSSDD98 pKa = 3.13EE99 pKa = 4.08TSPLEE104 pKa = 3.95LTVGGVVLEE113 pKa = 4.31HH114 pKa = 6.29VGGVVSSNEE123 pKa = 3.67WVIDD127 pKa = 4.14GNDD130 pKa = 3.46LDD132 pKa = 3.99VWSGEE137 pKa = 3.76GDD139 pKa = 3.83SQNEE143 pKa = 4.26TTNSSEE149 pKa = 4.49TVDD152 pKa = 3.72TDD154 pKa = 3.87SDD156 pKa = 3.84HH157 pKa = 7.3CVV159 pKa = 2.8

Molecular weight: 17.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4YB56|C4YB56_CLAL4 RFX-type winged-helix domain-containing protein OS=Clavispora lusitaniae (strain ATCC 42720) OX=306902 GN=CLUG_05348 PE=4 SV=1
SS1 pKa = 6.33RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84SRR8 pKa = 11.84GKK10 pKa = 9.82RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 9.3EE16 pKa = 3.12KK17 pKa = 9.16RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 7.75RR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 8.35RR28 pKa = 11.84RR29 pKa = 11.84GRR31 pKa = 11.84SRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84TRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84KK42 pKa = 8.83RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84KK48 pKa = 8.06KK49 pKa = 7.86RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84EE56 pKa = 3.24KK57 pKa = 9.37RR58 pKa = 11.84RR59 pKa = 11.84KK60 pKa = 8.95RR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84KK64 pKa = 8.21KK65 pKa = 9.45KK66 pKa = 7.89EE67 pKa = 3.23RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84TRR72 pKa = 11.84RR73 pKa = 11.84SSKK76 pKa = 8.83RR77 pKa = 11.84RR78 pKa = 11.84SRR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84QSLTSLSTRR91 pKa = 11.84KK92 pKa = 9.67RR93 pKa = 11.84LSLTTRR99 pKa = 11.84RR100 pKa = 11.84MPLKK104 pKa = 9.88TRR106 pKa = 11.84RR107 pKa = 11.84TLSWTRR113 pKa = 11.84RR114 pKa = 11.84KK115 pKa = 10.05SS116 pKa = 3.25

Molecular weight: 15.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5932

0

5932

2742050

28

4176

462.2

51.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.068 ± 0.037

1.293 ± 0.011

5.62 ± 0.025

6.6 ± 0.035

4.468 ± 0.023

5.503 ± 0.035

2.411 ± 0.014

5.287 ± 0.028

6.334 ± 0.033

9.271 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.073 ± 0.012

4.672 ± 0.027

4.764 ± 0.03

3.903 ± 0.025

5.02 ± 0.025

9.513 ± 0.057

5.467 ± 0.035

6.529 ± 0.03

1.091 ± 0.01

3.115 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski