Streptococcus phage SPQS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saphexavirus; Streptococcus virus SPQS1

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6CQD2|S6CQD2_9CAUD Uncharacterized protein OS=Streptococcus phage SPQS1 OX=1208587 GN=gp60 PE=4 SV=1
MM1 pKa = 7.94IEE3 pKa = 4.22LFKK6 pKa = 11.54DD7 pKa = 3.31NLTLDD12 pKa = 3.72EE13 pKa = 5.09MSEE16 pKa = 3.58IWYY19 pKa = 9.15GWDD22 pKa = 4.23NEE24 pKa = 3.9GLLYY28 pKa = 10.6GWEE31 pKa = 3.94NGEE34 pKa = 4.07AGEE37 pKa = 4.79AYY39 pKa = 10.4ALLYY43 pKa = 10.03EE44 pKa = 4.79KK45 pKa = 10.14GYY47 pKa = 11.44NMDD50 pKa = 4.11SIKK53 pKa = 10.61VLHH56 pKa = 6.42EE57 pKa = 4.26AFDD60 pKa = 4.49TIGEE64 pKa = 4.39LIGAII69 pKa = 3.92

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6CRF8|S6CRF8_9CAUD Uncharacterized protein OS=Streptococcus phage SPQS1 OX=1208587 GN=gp96 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.74CLSDD6 pKa = 3.49SCRR9 pKa = 11.84NSFRR13 pKa = 11.84MPVGTAVGTPYY24 pKa = 10.48RR25 pKa = 11.84GFSRR29 pKa = 11.84KK30 pKa = 9.43YY31 pKa = 9.61KK32 pKa = 10.12SEE34 pKa = 3.91AYY36 pKa = 10.26NVGFPKK42 pKa = 10.64NCVRR46 pKa = 11.84WTVARR51 pKa = 11.84PARR54 pKa = 11.84PRR56 pKa = 11.84KK57 pKa = 9.66SYY59 pKa = 9.99PLPFPKK65 pKa = 10.07TPARR69 pKa = 11.84RR70 pKa = 11.84GHH72 pKa = 6.2ACALRR77 pKa = 11.84LPSRR81 pKa = 11.84FEE83 pKa = 3.87RR84 pKa = 11.84RR85 pKa = 11.84CEE87 pKa = 3.78YY88 pKa = 10.66SATLSRR94 pKa = 11.84HH95 pKa = 5.04AVRR98 pKa = 11.84HH99 pKa = 4.88AHH101 pKa = 6.52SSDD104 pKa = 3.14SMM106 pKa = 3.88

Molecular weight: 12.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

17408

34

1008

167.4

19.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.169 ± 0.515

0.764 ± 0.098

6.049 ± 0.223

8.892 ± 0.414

4.285 ± 0.217

5.946 ± 0.397

1.557 ± 0.109

6.307 ± 0.244

7.847 ± 0.254

8.479 ± 0.327

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.688 ± 0.141

5.021 ± 0.189

3.079 ± 0.21

3.539 ± 0.287

4.148 ± 0.217

6.089 ± 0.205

6.032 ± 0.322

6.537 ± 0.213

1.281 ± 0.115

4.291 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski