Rubrimonas cliftonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rubrimonas

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4574 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3YZ09|A0A1H3YZ09_9RHOB Glycosyltransferase catalytic subunit of cellulose synthase and poly-beta-1 6-N-acetylglucosamine synthase OS=Rubrimonas cliftonensis OX=89524 GN=SAMN05444370_103298 PE=4 SV=1
MM1 pKa = 7.23VSVSHH6 pKa = 6.78AAAAAAVVSMSLAFATSAGAALAPAFVYY34 pKa = 9.59TGSVGVSTDD43 pKa = 3.25GFGSTSGVGTISASAPSGSTVIAAYY68 pKa = 10.15LYY70 pKa = 9.08TSTYY74 pKa = 11.08DD75 pKa = 3.22EE76 pKa = 4.73VGFSTVAPTTVTLDD90 pKa = 3.45GDD92 pKa = 4.03AVTYY96 pKa = 7.4TQSIQNPSYY105 pKa = 11.37SRR107 pKa = 11.84LTAHH111 pKa = 7.23RR112 pKa = 11.84ADD114 pKa = 3.48VTSTVKK120 pKa = 10.43PVIDD124 pKa = 4.51GSAGGVYY131 pKa = 10.44DD132 pKa = 4.56FAYY135 pKa = 10.69NEE137 pKa = 3.96NNAGVEE143 pKa = 4.0NGRR146 pKa = 11.84TGIDD150 pKa = 3.17GSALVVVYY158 pKa = 9.71DD159 pKa = 4.43NPALSDD165 pKa = 3.33RR166 pKa = 11.84TVAVLDD172 pKa = 4.04GFSDD176 pKa = 3.44IAGDD180 pKa = 3.8TFDD183 pKa = 3.52VTFGVPLDD191 pKa = 3.6PTAPGFNLEE200 pKa = 4.07MFLGIGYY207 pKa = 8.26SCCNQASNITVNGTLISRR225 pKa = 11.84NSGNFDD231 pKa = 5.24DD232 pKa = 6.49GLQQANGSLITMGSFDD248 pKa = 4.58DD249 pKa = 4.67APSAFLPSYY258 pKa = 10.64AADD261 pKa = 3.47TEE263 pKa = 4.54RR264 pKa = 11.84YY265 pKa = 10.05DD266 pKa = 3.74LAPYY270 pKa = 10.02VNAGDD275 pKa = 3.95TLLSVDD281 pKa = 4.04TLNPSVNDD289 pKa = 3.83NIFVAVLDD297 pKa = 4.02LTGTASVVNPDD308 pKa = 3.56VPTDD312 pKa = 3.63PTVPPIPLPASMWLLVSGIAMAAGVARR339 pKa = 11.84RR340 pKa = 11.84RR341 pKa = 11.84ACC343 pKa = 3.8

Molecular weight: 34.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3ZA24|A0A1H3ZA24_9RHOB Gamma-glutamyl phosphate reductase OS=Rubrimonas cliftonensis OX=89524 GN=proA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27NGRR28 pKa = 11.84KK29 pKa = 8.92IVNARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84LGRR39 pKa = 11.84KK40 pKa = 8.88HH41 pKa = 5.54LTAA44 pKa = 5.64

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4574

0

4574

1452153

26

3054

317.5

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

16.617 ± 0.075

0.868 ± 0.012

5.827 ± 0.042

5.697 ± 0.029

3.489 ± 0.025

9.413 ± 0.037

1.773 ± 0.015

3.934 ± 0.025

1.951 ± 0.025

10.218 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.017

1.855 ± 0.019

5.663 ± 0.026

2.34 ± 0.017

8.122 ± 0.047

4.478 ± 0.025

4.747 ± 0.025

7.41 ± 0.031

1.42 ± 0.015

1.768 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski