Leuconostoc phage 1-A4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mccleskeyvirinae; Unaquatrovirus; Leuconostoc virus 1A4

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4N4M2|D4N4M2_9CAUD Phage-related protein OS=Leuconostoc phage 1-A4 OX=745088 GN=LM1A4_049 PE=4 SV=1
MM1 pKa = 7.78TIQDD5 pKa = 3.69DD6 pKa = 4.14KK7 pKa = 11.79EE8 pKa = 4.49NGVEE12 pKa = 3.98NYY14 pKa = 10.04RR15 pKa = 11.84GFSILFSDD23 pKa = 4.32EE24 pKa = 4.19DD25 pKa = 4.04WYY27 pKa = 9.24HH28 pKa = 5.75TNYY31 pKa = 10.25VSGIGMFSNFYY42 pKa = 10.8SIDD45 pKa = 3.72SLIEE49 pKa = 4.52SIDD52 pKa = 3.47EE53 pKa = 4.41CLDD56 pKa = 4.83KK57 pKa = 11.0PIEE60 pKa = 4.14WYY62 pKa = 10.22KK63 pKa = 11.32

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4N4J1|D4N4J1_9CAUD Major tail protein OS=Leuconostoc phage 1-A4 OX=745088 GN=LM1A4_018 PE=4 SV=1
MM1 pKa = 7.26SAKK4 pKa = 10.39KK5 pKa = 9.95IDD7 pKa = 3.55ISDD10 pKa = 3.85LTKK13 pKa = 10.81LPPRR17 pKa = 11.84LMTAKK22 pKa = 10.08ILEE25 pKa = 4.33KK26 pKa = 10.26RR27 pKa = 11.84DD28 pKa = 3.88DD29 pKa = 3.7VAKK32 pKa = 10.62QAGSDD37 pKa = 3.46MTSALSVWRR46 pKa = 11.84STAPIKK52 pKa = 8.94TGRR55 pKa = 11.84MRR57 pKa = 11.84STMTLNRR64 pKa = 11.84GKK66 pKa = 10.55YY67 pKa = 7.98GATMVPRR74 pKa = 11.84DD75 pKa = 3.53SYY77 pKa = 10.49MKK79 pKa = 10.56VVNAVNKK86 pKa = 9.86RR87 pKa = 11.84GKK89 pKa = 9.44HH90 pKa = 4.14QGFLDD95 pKa = 3.59RR96 pKa = 11.84FKK98 pKa = 10.86RR99 pKa = 11.84GQGKK103 pKa = 9.58SFLQKK108 pKa = 9.19WW109 pKa = 3.4

Molecular weight: 12.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

9023

39

889

180.5

20.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.83 ± 0.544

0.332 ± 0.082

6.539 ± 0.31

6.594 ± 0.496

4.622 ± 0.228

6.683 ± 0.526

1.385 ± 0.209

6.993 ± 0.313

7.503 ± 0.485

8.201 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.218

6.495 ± 0.194

2.682 ± 0.206

3.635 ± 0.233

3.524 ± 0.254

6.971 ± 0.645

7.215 ± 0.387

6.594 ± 0.278

1.097 ± 0.13

4.378 ± 0.351
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski