Clostridium aceticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3835 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D8IBX6|A0A0D8IBX6_9CLOT Uncharacterized protein OS=Clostridium aceticum OX=84022 GN=CACET_c22820 PE=4 SV=1
MM1 pKa = 7.53SYY3 pKa = 10.54LRR5 pKa = 11.84KK6 pKa = 9.54RR7 pKa = 11.84LYY9 pKa = 10.34RR10 pKa = 11.84KK11 pKa = 9.84ISITVLLILSLVVMAACSPEE31 pKa = 4.03GTDD34 pKa = 3.85EE35 pKa = 5.34RR36 pKa = 11.84IVLADD41 pKa = 4.28AGWDD45 pKa = 3.14SLAFHH50 pKa = 6.85NEE52 pKa = 3.16VAALIIEE59 pKa = 4.25NGYY62 pKa = 10.69GYY64 pKa = 8.67STYY67 pKa = 10.23IEE69 pKa = 4.39MGSTPITFAALRR81 pKa = 11.84NGSIDD86 pKa = 3.2VYY88 pKa = 10.82MEE90 pKa = 4.39LWTDD94 pKa = 3.88NIIEE98 pKa = 4.95AYY100 pKa = 9.46TEE102 pKa = 3.93ALEE105 pKa = 4.37EE106 pKa = 4.17GDD108 pKa = 3.8IIEE111 pKa = 4.44VSTNFDD117 pKa = 3.79DD118 pKa = 4.34NAQGLYY124 pKa = 10.0VPTYY128 pKa = 10.12VIEE131 pKa = 4.77GDD133 pKa = 3.43AEE135 pKa = 4.16RR136 pKa = 11.84GIEE139 pKa = 3.99PMAPGLRR146 pKa = 11.84SVQDD150 pKa = 3.74LPDD153 pKa = 3.25YY154 pKa = 10.93WEE156 pKa = 4.14VFKK159 pKa = 11.3DD160 pKa = 3.88PEE162 pKa = 4.5DD163 pKa = 3.64SSKK166 pKa = 10.93GRR168 pKa = 11.84IYY170 pKa = 10.85GAIPGWEE177 pKa = 3.94VDD179 pKa = 4.4EE180 pKa = 5.3ILQQKK185 pKa = 9.3VKK187 pKa = 9.11THH189 pKa = 6.25GLDD192 pKa = 2.86EE193 pKa = 4.6NYY195 pKa = 10.85NYY197 pKa = 10.05FSPGSDD203 pKa = 2.9TALGSSIIAAIEE215 pKa = 3.99RR216 pKa = 11.84GEE218 pKa = 4.01AWLGYY223 pKa = 8.21YY224 pKa = 8.93WEE226 pKa = 4.43PTWIIGMYY234 pKa = 10.93DD235 pKa = 3.21MTLLEE240 pKa = 5.57DD241 pKa = 3.33IPYY244 pKa = 10.67DD245 pKa = 3.65EE246 pKa = 5.39EE247 pKa = 4.08KK248 pKa = 10.72WNDD251 pKa = 3.49GYY253 pKa = 10.42ATEE256 pKa = 4.45MPAVDD261 pKa = 3.5VTVAVHH267 pKa = 7.07KK268 pKa = 10.66DD269 pKa = 3.45MPEE272 pKa = 3.84KK273 pKa = 10.65APEE276 pKa = 3.61IVAFLEE282 pKa = 4.37NYY284 pKa = 6.14QTSSAVTSEE293 pKa = 3.66ALAYY297 pKa = 8.45MQANDD302 pKa = 3.5ATTEE306 pKa = 3.92EE307 pKa = 4.08AAVWFLKK314 pKa = 10.31EE315 pKa = 5.32YY316 pKa = 10.48EE317 pKa = 4.59DD318 pKa = 5.73LWTAWVPEE326 pKa = 4.28EE327 pKa = 3.64VAEE330 pKa = 4.11AVKK333 pKa = 10.33EE334 pKa = 4.22AIQQ337 pKa = 3.64

Molecular weight: 37.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D8IAJ2|A0A0D8IAJ2_9CLOT Uncharacterized protein OS=Clostridium aceticum OX=84022 GN=CACET_c25770 PE=4 SV=1
MM1 pKa = 7.34MGDD4 pKa = 3.26KK5 pKa = 11.05LNIIRR10 pKa = 11.84LGYY13 pKa = 9.91RR14 pKa = 11.84KK15 pKa = 9.52RR16 pKa = 11.84QIRR19 pKa = 11.84WIVVTLILATITLSLCVTLLLLGNSKK45 pKa = 10.84YY46 pKa = 10.66SLEE49 pKa = 4.1VVIRR53 pKa = 11.84VLLGEE58 pKa = 4.91EE59 pKa = 3.72IQGASFAISTLRR71 pKa = 11.84LPRR74 pKa = 11.84MLAGLLVGIGFGMAGSTFQTILRR97 pKa = 11.84NPLASPDD104 pKa = 3.96IIGVTAGSSVGAMFCILVLKK124 pKa = 10.23MSGTIVSVVSVISGLLVAVLIYY146 pKa = 10.72LFSKK150 pKa = 10.8VGGFSGGKK158 pKa = 9.8LILIGIGIQAMLNAVVSFLLLKK180 pKa = 10.52APQYY184 pKa = 10.29DD185 pKa = 3.46VPAAYY190 pKa = 9.41RR191 pKa = 11.84WLSGSLNGIQMTNIPSLFIVLVIFGSVIILLGRR224 pKa = 11.84HH225 pKa = 5.73LKK227 pKa = 10.1ILEE230 pKa = 4.64LGEE233 pKa = 4.16QSATTLGVRR242 pKa = 11.84ADD244 pKa = 3.8LVRR247 pKa = 11.84FLLVLSAVFLIAFATAVTGPISFVAFLAGPIATRR281 pKa = 11.84VVGSGAPNEE290 pKa = 4.3FPAGLVGAILVLGADD305 pKa = 5.37LIGQFAFGTRR315 pKa = 11.84FPVGVITGILGAPYY329 pKa = 10.48LIILLIRR336 pKa = 11.84MNRR339 pKa = 11.84TGGSAA344 pKa = 3.0

Molecular weight: 36.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3835

0

3835

1179242

14

2831

307.5

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.535 ± 0.046

0.99 ± 0.017

5.029 ± 0.034

7.851 ± 0.046

4.161 ± 0.03

6.774 ± 0.045

1.787 ± 0.016

9.243 ± 0.039

7.789 ± 0.048

9.589 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.849 ± 0.019

4.789 ± 0.026

3.21 ± 0.028

3.266 ± 0.02

3.802 ± 0.026

5.648 ± 0.03

5.27 ± 0.036

6.9 ± 0.037

0.768 ± 0.015

3.75 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski