Amino acid dipepetide frequency for Halorubrum sp. E3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.896AlaAla: 13.896 ± 0.159	0.672AlaCys: 0.672 ± 0.027	10.453AlaAsp: 10.453 ± 0.146	9.134AlaGlu: 9.134 ± 0.116	4.027AlaPhe: 4.027 ± 0.071	9.871AlaGly: 9.871 ± 0.109	1.737AlaHis: 1.737 ± 0.038	4.763AlaIle: 4.763 ± 0.076	1.929AlaLys: 1.929 ± 0.047	10.173AlaLeu: 10.173 ± 0.109	2.077AlaMet: 2.077 ± 0.046	2.451AlaAsn: 2.451 ± 0.046	4.097AlaPro: 4.097 ± 0.072	2.092AlaGln: 2.092 ± 0.043	5.932AlaArg: 5.932 ± 0.082	5.377AlaSer: 5.377 ± 0.093	6.712AlaThr: 6.712 ± 0.092	11.059AlaVal: 11.059 ± 0.116	1.127AlaTrp: 1.127 ± 0.033	2.926AlaTyr: 2.926 ± 0.061	0.001AlaXaa: 0.001 ± 0.001
Cys	0.598CysAla: 0.598 ± 0.026	0.094CysCys: 0.094 ± 0.011	0.58CysAsp: 0.58 ± 0.024	0.598CysGlu: 0.598 ± 0.026	0.192CysPhe: 0.192 ± 0.014	0.81CysGly: 0.81 ± 0.033	0.183CysHis: 0.183 ± 0.014	0.212CysIle: 0.212 ± 0.014	0.126CysLys: 0.126 ± 0.012	0.481CysLeu: 0.481 ± 0.02	0.092CysMet: 0.092 ± 0.011	0.187CysAsn: 0.187 ± 0.014	0.543CysPro: 0.543 ± 0.025	0.17CysGln: 0.17 ± 0.014	0.463CysArg: 0.463 ± 0.025	0.43CysSer: 0.43 ± 0.023	0.387CysThr: 0.387 ± 0.018	0.501CysVal: 0.501 ± 0.024	0.09CysTrp: 0.09 ± 0.01	0.189CysTyr: 0.189 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	11.418AspAla: 11.418 ± 0.148	0.577AspCys: 0.577 ± 0.028	8.62AspAsp: 8.62 ± 0.113	8.534AspGlu: 8.534 ± 0.107	1.945AspPhe: 1.945 ± 0.039	8.763AspGly: 8.763 ± 0.12	1.921AspHis: 1.921 ± 0.049	3.071AspIle: 3.071 ± 0.064	1.055AspLys: 1.055 ± 0.033	7.465AspLeu: 7.465 ± 0.087	1.164AspMet: 1.164 ± 0.036	1.377AspAsn: 1.377 ± 0.038	5.023AspPro: 5.023 ± 0.076	1.805AspGln: 1.805 ± 0.046	7.18AspArg: 7.18 ± 0.099	3.68AspSer: 3.68 ± 0.058	4.174AspThr: 4.174 ± 0.066	7.763AspVal: 7.763 ± 0.104	1.022AspTrp: 1.022 ± 0.033	1.818AspTyr: 1.818 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	9.212GluAla: 9.212 ± 0.122	0.536GluCys: 0.536 ± 0.023	5.427GluAsp: 5.427 ± 0.077	7.035GluGlu: 7.035 ± 0.112	3.011GluPhe: 3.011 ± 0.052	5.857GluGly: 5.857 ± 0.072	1.851GluHis: 1.851 ± 0.045	3.979GluIle: 3.979 ± 0.075	1.902GluLys: 1.902 ± 0.049	7.324GluLeu: 7.324 ± 0.1	1.872GluMet: 1.872 ± 0.046	2.225GluAsn: 2.225 ± 0.048	4.025GluPro: 4.025 ± 0.083	2.456GluGln: 2.456 ± 0.049	7.505GluArg: 7.505 ± 0.086	5.276GluSer: 5.276 ± 0.074	6.412GluThr: 6.412 ± 0.089	5.999GluVal: 5.999 ± 0.091	1.153GluTrp: 1.153 ± 0.041	2.751GluTyr: 2.751 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	3.69PheAla: 3.69 ± 0.073	0.287PheCys: 0.287 ± 0.018	3.376PheAsp: 3.376 ± 0.065	3.049PheGlu: 3.049 ± 0.051	0.991PhePhe: 0.991 ± 0.041	3.162PheGly: 3.162 ± 0.069	0.63PheHis: 0.63 ± 0.027	1.064PheIle: 1.064 ± 0.032	0.51PheLys: 0.51 ± 0.023	2.903PheLeu: 2.903 ± 0.06	0.518PheMet: 0.518 ± 0.022	0.752PheAsn: 0.752 ± 0.029	1.327PhePro: 1.327 ± 0.04	0.762PheGln: 0.762 ± 0.025	1.863PheArg: 1.863 ± 0.044	1.658PheSer: 1.658 ± 0.044	1.855PheThr: 1.855 ± 0.045	3.102PheVal: 3.102 ± 0.057	0.389PheTrp: 0.389 ± 0.02	0.851PheTyr: 0.851 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	8.413GlyAla: 8.413 ± 0.119	0.645GlyCys: 0.645 ± 0.028	7.689GlyAsp: 7.689 ± 0.113	7.473GlyGlu: 7.473 ± 0.087	3.143GlyPhe: 3.143 ± 0.059	7.78GlyGly: 7.78 ± 0.113	1.585GlyHis: 1.585 ± 0.042	4.159GlyIle: 4.159 ± 0.071	1.894GlyLys: 1.894 ± 0.041	7.029GlyLeu: 7.029 ± 0.1	1.655GlyMet: 1.655 ± 0.039	1.982GlyAsn: 1.982 ± 0.052	3.348GlyPro: 3.348 ± 0.069	1.819GlyGln: 1.819 ± 0.043	4.968GlyArg: 4.968 ± 0.077	5.319GlySer: 5.319 ± 0.095	5.394GlyThr: 5.394 ± 0.079	7.852GlyVal: 7.852 ± 0.103	1.092GlyTrp: 1.092 ± 0.038	2.708GlyTyr: 2.708 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.015HisAla: 2.015 ± 0.052	0.185HisCys: 0.185 ± 0.012	1.767HisAsp: 1.767 ± 0.046	1.619HisGlu: 1.619 ± 0.044	0.532HisPhe: 0.532 ± 0.024	1.865HisGly: 1.865 ± 0.051	0.52HisHis: 0.52 ± 0.026	0.648HisIle: 0.648 ± 0.025	0.321HisLys: 0.321 ± 0.018	1.624HisLeu: 1.624 ± 0.043	0.291HisMet: 0.291 ± 0.017	0.476HisAsn: 0.476 ± 0.019	1.185HisPro: 1.185 ± 0.036	0.484HisGln: 0.484 ± 0.022	1.381HisArg: 1.381 ± 0.04	0.872HisSer: 0.872 ± 0.03	1.064HisThr: 1.064 ± 0.035	1.803HisVal: 1.803 ± 0.048	0.219HisTrp: 0.219 ± 0.014	0.608HisTyr: 0.608 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	4.995IleAla: 4.995 ± 0.073	0.273IleCys: 0.273 ± 0.016	4.224IleAsp: 4.224 ± 0.067	4.228IleGlu: 4.228 ± 0.066	0.981IlePhe: 0.981 ± 0.032	3.804IleGly: 3.804 ± 0.07	0.842IleHis: 0.842 ± 0.029	1.367IleIle: 1.367 ± 0.043	0.788IleLys: 0.788 ± 0.029	2.912IleLeu: 2.912 ± 0.068	0.494IleMet: 0.494 ± 0.025	1.06IleAsn: 1.06 ± 0.032	2.124IlePro: 2.124 ± 0.042	1.128IleGln: 1.128 ± 0.04	2.528IleArg: 2.528 ± 0.046	2.16IleSer: 2.16 ± 0.051	2.3IleThr: 2.3 ± 0.056	3.624IleVal: 3.624 ± 0.059	0.345IleTrp: 0.345 ± 0.018	0.898IleTyr: 0.898 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	1.936LysAla: 1.936 ± 0.046	0.136LysCys: 0.136 ± 0.012	1.051LysAsp: 1.051 ± 0.036	1.471LysGlu: 1.471 ± 0.045	0.549LysPhe: 0.549 ± 0.025	1.404LysGly: 1.404 ± 0.039	0.514LysHis: 0.514 ± 0.025	0.77LysIle: 0.77 ± 0.031	0.575LysLys: 0.575 ± 0.026	1.754LysLeu: 1.754 ± 0.047	0.384LysMet: 0.384 ± 0.017	0.633LysAsn: 0.633 ± 0.028	0.984LysPro: 0.984 ± 0.029	0.853LysGln: 0.853 ± 0.035	1.832LysArg: 1.832 ± 0.041	1.247LysSer: 1.247 ± 0.039	1.376LysThr: 1.376 ± 0.041	1.185LysVal: 1.185 ± 0.036	0.24LysTrp: 0.24 ± 0.014	0.601LysTyr: 0.601 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	10.333LeuAla: 10.333 ± 0.109	0.562LeuCys: 0.562 ± 0.021	7.864LeuAsp: 7.864 ± 0.099	5.885LeuGlu: 5.885 ± 0.086	3.023LeuPhe: 3.023 ± 0.068	7.232LeuGly: 7.232 ± 0.083	1.421LeuHis: 1.421 ± 0.037	3.294LeuIle: 3.294 ± 0.061	1.788LeuLys: 1.788 ± 0.048	7.995LeuLeu: 7.995 ± 0.109	1.32LeuMet: 1.32 ± 0.037	1.965LeuAsn: 1.965 ± 0.047	4.082LeuPro: 4.082 ± 0.062	2.009LeuGln: 2.009 ± 0.041	5.866LeuArg: 5.866 ± 0.078	5.615LeuSer: 5.615 ± 0.08	5.18LeuThr: 5.18 ± 0.076	7.782LeuVal: 7.782 ± 0.102	0.865LeuTrp: 0.865 ± 0.03	2.322LeuTyr: 2.322 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	1.748MetAla: 1.748 ± 0.042	0.102MetCys: 0.102 ± 0.01	1.263MetAsp: 1.263 ± 0.032	1.171MetGlu: 1.171 ± 0.038	0.471MetPhe: 0.471 ± 0.019	1.337MetGly: 1.337 ± 0.041	0.275MetHis: 0.275 ± 0.017	0.656MetIle: 0.656 ± 0.027	0.471MetLys: 0.471 ± 0.023	1.442MetLeu: 1.442 ± 0.034	0.314MetMet: 0.314 ± 0.017	0.576MetAsn: 0.576 ± 0.026	0.804MetPro: 0.804 ± 0.029	0.377MetGln: 0.377 ± 0.021	1.084MetArg: 1.084 ± 0.028	1.6MetSer: 1.6 ± 0.044	1.432MetThr: 1.432 ± 0.041	1.221MetVal: 1.221 ± 0.035	0.173MetTrp: 0.173 ± 0.013	0.447MetTyr: 0.447 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.699AsnAla: 2.699 ± 0.056	0.22AsnCys: 0.22 ± 0.014	1.77AsnAsp: 1.77 ± 0.045	1.781AsnGlu: 1.781 ± 0.052	0.677AsnPhe: 0.677 ± 0.024	2.133AsnGly: 2.133 ± 0.054	0.488AsnHis: 0.488 ± 0.021	0.944AsnIle: 0.944 ± 0.033	0.497AsnLys: 0.497 ± 0.026	1.988AsnLeu: 1.988 ± 0.047	0.389AsnMet: 0.389 ± 0.02	0.62AsnAsn: 0.62 ± 0.028	1.559AsnPro: 1.559 ± 0.041	0.712AsnGln: 0.712 ± 0.029	1.62AsnArg: 1.62 ± 0.041	1.077AsnSer: 1.077 ± 0.03	1.369AsnThr: 1.369 ± 0.037	2.316AsnVal: 2.316 ± 0.053	0.313AsnTrp: 0.313 ± 0.017	0.708AsnTyr: 0.708 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	4.714ProAla: 4.714 ± 0.077	0.233ProCys: 0.233 ± 0.015	5.373ProAsp: 5.373 ± 0.081	4.774ProGlu: 4.774 ± 0.07	1.565ProPhe: 1.565 ± 0.042	3.709ProGly: 3.709 ± 0.07	0.924ProHis: 0.924 ± 0.03	1.9ProIle: 1.9 ± 0.047	0.897ProLys: 0.897 ± 0.031	3.554ProLeu: 3.554 ± 0.059	0.751ProMet: 0.751 ± 0.027	1.164ProAsn: 1.164 ± 0.033	2.178ProPro: 2.178 ± 0.054	0.941ProGln: 0.941 ± 0.034	2.311ProArg: 2.311 ± 0.049	2.801ProSer: 2.801 ± 0.053	3.056ProThr: 3.056 ± 0.063	4.18ProVal: 4.18 ± 0.067	0.491ProTrp: 0.491 ± 0.021	1.19ProTyr: 1.19 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.237GlnAla: 2.237 ± 0.05	0.153GlnCys: 0.153 ± 0.012	1.283GlnAsp: 1.283 ± 0.035	1.748GlnGlu: 1.748 ± 0.048	0.991GlnPhe: 0.991 ± 0.03	1.623GlnGly: 1.623 ± 0.043	0.547GlnHis: 0.547 ± 0.024	1.177GlnIle: 1.177 ± 0.035	0.663GlnLys: 0.663 ± 0.028	2.276GlnLeu: 2.276 ± 0.053	0.524GlnMet: 0.524 ± 0.025	0.782GlnAsn: 0.782 ± 0.029	1.063GlnPro: 1.063 ± 0.033	0.988GlnGln: 0.988 ± 0.033	1.918GlnArg: 1.918 ± 0.043	1.61GlnSer: 1.61 ± 0.037	1.637GlnThr: 1.637 ± 0.04	1.62GlnVal: 1.62 ± 0.041	0.341GlnTrp: 0.341 ± 0.02	0.849GlnTyr: 0.849 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	6.249ArgAla: 6.249 ± 0.092	0.517ArgCys: 0.517 ± 0.025	5.569ArgAsp: 5.569 ± 0.084	6.902ArgGlu: 6.902 ± 0.092	2.484ArgPhe: 2.484 ± 0.053	4.689ArgGly: 4.689 ± 0.07	1.267ArgHis: 1.267 ± 0.039	3.066ArgIle: 3.066 ± 0.064	1.46ArgLys: 1.46 ± 0.037	6.088ArgLeu: 6.088 ± 0.084	1.2ArgMet: 1.2 ± 0.032	1.712ArgAsn: 1.712 ± 0.047	2.571ArgPro: 2.571 ± 0.047	1.72ArgGln: 1.72 ± 0.041	4.914ArgArg: 4.914 ± 0.089	3.634ArgSer: 3.634 ± 0.065	4.015ArgThr: 4.015 ± 0.062	5.563ArgVal: 5.563 ± 0.081	0.786ArgTrp: 0.786 ± 0.031	1.991ArgTyr: 1.991 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	5.407SerAla: 5.407 ± 0.083	0.355SerCys: 0.355 ± 0.019	4.83SerAsp: 4.83 ± 0.079	4.402SerGlu: 4.402 ± 0.068	1.966SerPhe: 1.966 ± 0.055	5.492SerGly: 5.492 ± 0.089	1.063SerHis: 1.063 ± 0.034	2.516SerIle: 2.516 ± 0.053	1.252SerLys: 1.252 ± 0.039	4.709SerLeu: 4.709 ± 0.072	1.001SerMet: 1.001 ± 0.032	1.346SerAsn: 1.346 ± 0.042	2.743SerPro: 2.743 ± 0.051	1.425SerGln: 1.425 ± 0.039	3.299SerArg: 3.299 ± 0.06	2.933SerSer: 2.933 ± 0.066	3.48SerThr: 3.48 ± 0.059	4.887SerVal: 4.887 ± 0.076	0.648SerTrp: 0.648 ± 0.029	1.481SerTyr: 1.481 ± 0.041	0.001SerXaa: 0.001 ± 0.001
Thr	6.791ThrAla: 6.791 ± 0.095	0.398ThrCys: 0.398 ± 0.023	5.595ThrAsp: 5.595 ± 0.074	4.681ThrGlu: 4.681 ± 0.063	2.073ThrPhe: 2.073 ± 0.047	5.708ThrGly: 5.708 ± 0.073	1.209ThrHis: 1.209 ± 0.041	2.698ThrIle: 2.698 ± 0.054	1.079ThrLys: 1.079 ± 0.033	5.597ThrLeu: 5.597 ± 0.078	0.978ThrMet: 0.978 ± 0.032	1.468ThrAsn: 1.468 ± 0.04	3.153ThrPro: 3.153 ± 0.062	1.376ThrGln: 1.376 ± 0.037	3.344ThrArg: 3.344 ± 0.06	2.898ThrSer: 2.898 ± 0.051	4.232ThrThr: 4.232 ± 0.086	6.783ThrVal: 6.783 ± 0.096	0.647ThrTrp: 0.647 ± 0.029	1.689ThrTyr: 1.689 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	10.309ValAla: 10.309 ± 0.108	0.635ValCys: 0.635 ± 0.026	8.23ValAsp: 8.23 ± 0.108	7.897ValGlu: 7.897 ± 0.108	2.846ValPhe: 2.846 ± 0.056	7.561ValGly: 7.561 ± 0.107	1.593ValHis: 1.593 ± 0.044	3.461ValIle: 3.461 ± 0.059	1.441ValLys: 1.441 ± 0.041	7.354ValLeu: 7.354 ± 0.086	1.343ValMet: 1.343 ± 0.034	2.049ValAsn: 2.049 ± 0.045	4.239ValPro: 4.239 ± 0.067	1.79ValGln: 1.79 ± 0.04	5.568ValArg: 5.568 ± 0.078	5.129ValSer: 5.129 ± 0.075	6.042ValThr: 6.042 ± 0.09	8.474ValVal: 8.474 ± 0.093	0.852ValTrp: 0.852 ± 0.029	2.115ValTyr: 2.115 ± 0.047	0.001ValXaa: 0.001 ± 0.001
Trp	0.982TrpAla: 0.982 ± 0.036	0.116TrpCys: 0.116 ± 0.011	0.761TrpAsp: 0.761 ± 0.029	0.916TrpGlu: 0.916 ± 0.027	0.416TrpPhe: 0.416 ± 0.021	0.807TrpGly: 0.807 ± 0.024	0.232TrpHis: 0.232 ± 0.016	0.538TrpIle: 0.538 ± 0.022	0.33TrpLys: 0.33 ± 0.018	1.161TrpLeu: 1.161 ± 0.033	0.241TrpMet: 0.241 ± 0.017	0.386TrpAsn: 0.386 ± 0.023	0.399TrpPro: 0.399 ± 0.02	0.343TrpGln: 0.343 ± 0.017	0.863TrpArg: 0.863 ± 0.028	0.661TrpSer: 0.661 ± 0.029	0.756TrpThr: 0.756 ± 0.028	0.824TrpVal: 0.824 ± 0.028	0.183TrpTrp: 0.183 ± 0.015	0.374TrpTyr: 0.374 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.866TyrAla: 2.866 ± 0.056	0.226TyrCys: 0.226 ± 0.016	2.652TyrAsp: 2.652 ± 0.061	2.509TyrGlu: 2.509 ± 0.049	0.856TyrPhe: 0.856 ± 0.03	2.34TyrGly: 2.34 ± 0.044	0.676TyrHis: 0.676 ± 0.031	0.792TyrIle: 0.792 ± 0.031	0.516TyrLys: 0.516 ± 0.027	2.575TyrLeu: 2.575 ± 0.047	0.352TyrMet: 0.352 ± 0.018	0.67TyrAsn: 0.67 ± 0.027	1.257TyrPro: 1.257 ± 0.04	0.781TyrGln: 0.781 ± 0.033	1.958TyrArg: 1.958 ± 0.044	1.26TyrSer: 1.26 ± 0.037	1.524TyrThr: 1.524 ± 0.038	2.39TyrVal: 2.39 ± 0.053	0.313TyrTrp: 0.313 ± 0.018	0.902TyrTyr: 0.902 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 4984 proteins (1007089 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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