Amino acid dipepetide frequency for Pseudomonas phage PMBT3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.906AlaAla: 13.906 ± 1.353
0.883AlaCys: 0.883 ± 0.165
6.328AlaAsp: 6.328 ± 0.46
7.505AlaGlu: 7.505 ± 0.763
2.281AlaPhe: 2.281 ± 0.245
6.953AlaGly: 6.953 ± 0.597
1.987AlaHis: 1.987 ± 0.28
5.187AlaIle: 5.187 ± 0.403
5.849AlaLys: 5.849 ± 0.581
9.197AlaLeu: 9.197 ± 0.551
2.833AlaMet: 2.833 ± 0.294
4.488AlaAsn: 4.488 ± 0.437
2.943AlaPro: 2.943 ± 0.302
5.592AlaGln: 5.592 ± 0.73
4.635AlaArg: 4.635 ± 0.418
5.702AlaSer: 5.702 ± 0.433
5.96AlaThr: 5.96 ± 0.49
6.696AlaVal: 6.696 ± 0.599
1.545AlaTrp: 1.545 ± 0.222
3.237AlaTyr: 3.237 ± 0.379
0.0AlaXaa: 0.0 ± 0.0
Cys
0.294CysAla: 0.294 ± 0.119
0.184CysCys: 0.184 ± 0.084
0.736CysAsp: 0.736 ± 0.148
0.993CysGlu: 0.993 ± 0.195
0.147CysPhe: 0.147 ± 0.076
0.883CysGly: 0.883 ± 0.202
0.368CysHis: 0.368 ± 0.132
0.699CysIle: 0.699 ± 0.167
0.625CysLys: 0.625 ± 0.146
0.589CysLeu: 0.589 ± 0.134
0.294CysMet: 0.294 ± 0.111
0.552CysAsn: 0.552 ± 0.137
0.699CysPro: 0.699 ± 0.166
0.294CysGln: 0.294 ± 0.108
0.294CysArg: 0.294 ± 0.11
0.625CysSer: 0.625 ± 0.14
0.589CysThr: 0.589 ± 0.142
0.736CysVal: 0.736 ± 0.166
0.258CysTrp: 0.258 ± 0.087
0.331CysTyr: 0.331 ± 0.111
0.0CysXaa: 0.0 ± 0.0
Asp
5.702AspAla: 5.702 ± 0.422
0.625AspCys: 0.625 ± 0.158
3.863AspAsp: 3.863 ± 0.364
4.525AspGlu: 4.525 ± 0.5
2.98AspPhe: 2.98 ± 0.306
5.482AspGly: 5.482 ± 0.573
1.472AspHis: 1.472 ± 0.233
3.752AspIle: 3.752 ± 0.388
3.716AspLys: 3.716 ± 0.43
5.666AspLeu: 5.666 ± 0.619
1.545AspMet: 1.545 ± 0.236
1.913AspAsn: 1.913 ± 0.23
2.281AspPro: 2.281 ± 0.322
2.281AspGln: 2.281 ± 0.24
2.906AspArg: 2.906 ± 0.421
2.906AspSer: 2.906 ± 0.256
3.716AspThr: 3.716 ± 0.286
3.9AspVal: 3.9 ± 0.321
1.03AspTrp: 1.03 ± 0.192
2.06AspTyr: 2.06 ± 0.334
0.0AspXaa: 0.0 ± 0.0
Glu
7.542GluAla: 7.542 ± 0.73
0.589GluCys: 0.589 ± 0.184
3.421GluAsp: 3.421 ± 0.269
5.187GluGlu: 5.187 ± 0.614
2.686GluPhe: 2.686 ± 0.398
4.01GluGly: 4.01 ± 0.406
1.324GluHis: 1.324 ± 0.239
3.605GluIle: 3.605 ± 0.339
4.12GluLys: 4.12 ± 0.405
6.548GluLeu: 6.548 ± 0.587
1.95GluMet: 1.95 ± 0.267
2.207GluAsn: 2.207 ± 0.282
2.575GluPro: 2.575 ± 0.312
3.311GluGln: 3.311 ± 0.374
3.274GluArg: 3.274 ± 0.379
2.649GluSer: 2.649 ± 0.328
2.906GluThr: 2.906 ± 0.374
5.334GluVal: 5.334 ± 0.584
1.324GluTrp: 1.324 ± 0.25
1.656GluTyr: 1.656 ± 0.234
0.0GluXaa: 0.0 ± 0.0
Phe
2.906PheAla: 2.906 ± 0.332
0.294PheCys: 0.294 ± 0.108
2.686PheAsp: 2.686 ± 0.272
2.354PheGlu: 2.354 ± 0.258
0.92PhePhe: 0.92 ± 0.223
2.649PheGly: 2.649 ± 0.302
0.662PheHis: 0.662 ± 0.154
1.508PheIle: 1.508 ± 0.271
1.876PheLys: 1.876 ± 0.308
1.692PheLeu: 1.692 ± 0.261
0.699PheMet: 0.699 ± 0.155
1.729PheAsn: 1.729 ± 0.265
1.435PhePro: 1.435 ± 0.276
1.03PheGln: 1.03 ± 0.194
1.803PheArg: 1.803 ± 0.234
2.318PheSer: 2.318 ± 0.301
2.538PheThr: 2.538 ± 0.353
2.171PheVal: 2.171 ± 0.301
0.294PheTrp: 0.294 ± 0.108
1.545PheTyr: 1.545 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
7.174GlyAla: 7.174 ± 0.615
0.662GlyCys: 0.662 ± 0.129
4.856GlyAsp: 4.856 ± 0.463
4.378GlyGlu: 4.378 ± 0.506
3.164GlyPhe: 3.164 ± 0.447
5.371GlyGly: 5.371 ± 0.654
1.214GlyHis: 1.214 ± 0.178
3.642GlyIle: 3.642 ± 0.325
4.488GlyLys: 4.488 ± 0.392
5.739GlyLeu: 5.739 ± 0.452
2.759GlyMet: 2.759 ± 0.398
2.649GlyAsn: 2.649 ± 0.269
2.428GlyPro: 2.428 ± 0.368
2.612GlyGln: 2.612 ± 0.397
3.274GlyArg: 3.274 ± 0.292
4.231GlySer: 4.231 ± 0.421
5.629GlyThr: 5.629 ± 0.579
5.298GlyVal: 5.298 ± 0.387
1.288GlyTrp: 1.288 ± 0.219
2.538GlyTyr: 2.538 ± 0.333
0.0GlyXaa: 0.0 ± 0.0
His
1.876HisAla: 1.876 ± 0.254
0.294HisCys: 0.294 ± 0.092
1.214HisAsp: 1.214 ± 0.278
1.582HisGlu: 1.582 ± 0.287
0.773HisPhe: 0.773 ± 0.173
1.803HisGly: 1.803 ± 0.288
0.258HisHis: 0.258 ± 0.101
1.067HisIle: 1.067 ± 0.192
1.214HisLys: 1.214 ± 0.21
1.582HisLeu: 1.582 ± 0.294
0.625HisMet: 0.625 ± 0.174
1.03HisAsn: 1.03 ± 0.149
1.251HisPro: 1.251 ± 0.214
0.699HisGln: 0.699 ± 0.171
1.067HisArg: 1.067 ± 0.18
0.846HisSer: 0.846 ± 0.138
1.251HisThr: 1.251 ± 0.261
0.957HisVal: 0.957 ± 0.171
0.552HisTrp: 0.552 ± 0.141
0.552HisTyr: 0.552 ± 0.144
0.0HisXaa: 0.0 ± 0.0
Ile
5.04IleAla: 5.04 ± 0.414
0.441IleCys: 0.441 ± 0.13
3.605IleAsp: 3.605 ± 0.462
3.201IleGlu: 3.201 ± 0.272
1.067IlePhe: 1.067 ± 0.201
3.752IleGly: 3.752 ± 0.391
1.214IleHis: 1.214 ± 0.233
2.502IleIle: 2.502 ± 0.349
2.759IleLys: 2.759 ± 0.28
3.679IleLeu: 3.679 ± 0.393
1.508IleMet: 1.508 ± 0.235
2.171IleAsn: 2.171 ± 0.305
2.686IlePro: 2.686 ± 0.326
2.281IleGln: 2.281 ± 0.246
2.833IleArg: 2.833 ± 0.351
2.98IleSer: 2.98 ± 0.358
4.562IleThr: 4.562 ± 0.482
3.237IleVal: 3.237 ± 0.362
0.441IleTrp: 0.441 ± 0.132
1.582IleTyr: 1.582 ± 0.232
0.0IleXaa: 0.0 ± 0.0
Lys
7.137LysAla: 7.137 ± 0.657
0.699LysCys: 0.699 ± 0.211
3.458LysAsp: 3.458 ± 0.413
3.936LysGlu: 3.936 ± 0.526
2.097LysPhe: 2.097 ± 0.297
3.532LysGly: 3.532 ± 0.363
1.324LysHis: 1.324 ± 0.204
2.87LysIle: 2.87 ± 0.319
3.679LysLys: 3.679 ± 0.467
5.371LysLeu: 5.371 ± 0.458
1.766LysMet: 1.766 ± 0.256
1.803LysAsn: 1.803 ± 0.239
2.722LysPro: 2.722 ± 0.304
2.281LysGln: 2.281 ± 0.316
3.237LysArg: 3.237 ± 0.39
3.053LysSer: 3.053 ± 0.364
2.98LysThr: 2.98 ± 0.367
4.451LysVal: 4.451 ± 0.408
0.625LysTrp: 0.625 ± 0.176
2.06LysTyr: 2.06 ± 0.3
0.0LysXaa: 0.0 ± 0.0
Leu
9.602LeuAla: 9.602 ± 0.802
0.736LeuCys: 0.736 ± 0.138
5.298LeuAsp: 5.298 ± 0.537
5.702LeuGlu: 5.702 ± 0.509
2.097LeuPhe: 2.097 ± 0.35
6.033LeuGly: 6.033 ± 0.426
1.987LeuHis: 1.987 ± 0.277
4.451LeuIle: 4.451 ± 0.445
5.518LeuLys: 5.518 ± 0.424
6.438LeuLeu: 6.438 ± 0.541
2.134LeuMet: 2.134 ± 0.273
3.311LeuAsn: 3.311 ± 0.375
3.936LeuPro: 3.936 ± 0.45
4.01LeuGln: 4.01 ± 0.498
4.746LeuArg: 4.746 ± 0.392
5.15LeuSer: 5.15 ± 0.288
5.776LeuThr: 5.776 ± 0.431
6.512LeuVal: 6.512 ± 0.422
0.625LeuTrp: 0.625 ± 0.159
2.354LeuTyr: 2.354 ± 0.3
0.0LeuXaa: 0.0 ± 0.0
Met
3.863MetAla: 3.863 ± 0.434
0.184MetCys: 0.184 ± 0.073
1.508MetAsp: 1.508 ± 0.202
1.766MetGlu: 1.766 ± 0.242
1.14MetPhe: 1.14 ± 0.19
1.508MetGly: 1.508 ± 0.2
0.441MetHis: 0.441 ± 0.115
1.766MetIle: 1.766 ± 0.238
1.656MetLys: 1.656 ± 0.27
2.502MetLeu: 2.502 ± 0.32
0.957MetMet: 0.957 ± 0.193
1.251MetAsn: 1.251 ± 0.175
1.104MetPro: 1.104 ± 0.19
1.508MetGln: 1.508 ± 0.269
1.361MetArg: 1.361 ± 0.235
1.876MetSer: 1.876 ± 0.279
1.766MetThr: 1.766 ± 0.27
1.692MetVal: 1.692 ± 0.226
0.221MetTrp: 0.221 ± 0.09
0.773MetTyr: 0.773 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
4.12AsnAla: 4.12 ± 0.461
0.221AsnCys: 0.221 ± 0.094
2.428AsnAsp: 2.428 ± 0.282
2.318AsnGlu: 2.318 ± 0.253
1.692AsnPhe: 1.692 ± 0.207
3.789AsnGly: 3.789 ± 0.454
0.92AsnHis: 0.92 ± 0.207
2.207AsnIle: 2.207 ± 0.281
1.913AsnLys: 1.913 ± 0.235
3.237AsnLeu: 3.237 ± 0.391
0.736AsnMet: 0.736 ± 0.175
1.656AsnAsn: 1.656 ± 0.281
2.281AsnPro: 2.281 ± 0.346
0.957AsnGln: 0.957 ± 0.206
1.766AsnArg: 1.766 ± 0.24
2.06AsnSer: 2.06 ± 0.249
2.281AsnThr: 2.281 ± 0.279
2.575AsnVal: 2.575 ± 0.304
0.699AsnTrp: 0.699 ± 0.142
1.177AsnTyr: 1.177 ± 0.207
0.0AsnXaa: 0.0 ± 0.0
Pro
3.752ProAla: 3.752 ± 0.338
0.515ProCys: 0.515 ± 0.15
2.796ProAsp: 2.796 ± 0.406
3.679ProGlu: 3.679 ± 0.338
1.288ProPhe: 1.288 ± 0.195
4.01ProGly: 4.01 ± 0.425
0.699ProHis: 0.699 ± 0.166
1.913ProIle: 1.913 ± 0.263
1.876ProLys: 1.876 ± 0.292
3.274ProLeu: 3.274 ± 0.432
1.361ProMet: 1.361 ± 0.246
1.545ProAsn: 1.545 ± 0.249
1.876ProPro: 1.876 ± 0.275
0.883ProGln: 0.883 ± 0.181
2.318ProArg: 2.318 ± 0.304
1.987ProSer: 1.987 ± 0.293
3.311ProThr: 3.311 ± 0.352
3.421ProVal: 3.421 ± 0.383
0.699ProTrp: 0.699 ± 0.167
1.324ProTyr: 1.324 ± 0.174
0.0ProXaa: 0.0 ± 0.0
Gln
4.93GlnAla: 4.93 ± 0.531
0.368GlnCys: 0.368 ± 0.114
1.656GlnAsp: 1.656 ± 0.267
2.023GlnGlu: 2.023 ± 0.286
1.361GlnPhe: 1.361 ± 0.212
2.906GlnGly: 2.906 ± 0.325
1.03GlnHis: 1.03 ± 0.227
2.06GlnIle: 2.06 ± 0.266
1.766GlnLys: 1.766 ± 0.306
4.893GlnLeu: 4.893 ± 0.671
1.435GlnMet: 1.435 ± 0.269
1.177GlnAsn: 1.177 ± 0.193
1.324GlnPro: 1.324 ± 0.242
1.619GlnGln: 1.619 ± 0.347
2.244GlnArg: 2.244 ± 0.299
1.987GlnSer: 1.987 ± 0.328
2.538GlnThr: 2.538 ± 0.351
3.017GlnVal: 3.017 ± 0.282
0.589GlnTrp: 0.589 ± 0.156
0.699GlnTyr: 0.699 ± 0.144
0.0GlnXaa: 0.0 ± 0.0
Arg
4.672ArgAla: 4.672 ± 0.474
0.515ArgCys: 0.515 ± 0.12
2.906ArgAsp: 2.906 ± 0.341
3.311ArgGlu: 3.311 ± 0.361
2.318ArgPhe: 2.318 ± 0.323
2.649ArgGly: 2.649 ± 0.342
1.361ArgHis: 1.361 ± 0.226
2.87ArgIle: 2.87 ± 0.335
3.569ArgLys: 3.569 ± 0.39
5.518ArgLeu: 5.518 ± 0.442
1.656ArgMet: 1.656 ± 0.255
1.839ArgAsn: 1.839 ± 0.199
1.95ArgPro: 1.95 ± 0.278
2.097ArgGln: 2.097 ± 0.289
2.686ArgArg: 2.686 ± 0.285
2.391ArgSer: 2.391 ± 0.308
2.98ArgThr: 2.98 ± 0.413
3.569ArgVal: 3.569 ± 0.394
0.699ArgTrp: 0.699 ± 0.157
1.398ArgTyr: 1.398 ± 0.216
0.0ArgXaa: 0.0 ± 0.0
Ser
5.077SerAla: 5.077 ± 0.473
0.662SerCys: 0.662 ± 0.18
3.458SerAsp: 3.458 ± 0.326
2.833SerGlu: 2.833 ± 0.269
1.656SerPhe: 1.656 ± 0.26
4.01SerGly: 4.01 ± 0.378
1.03SerHis: 1.03 ± 0.246
2.538SerIle: 2.538 ± 0.285
3.569SerLys: 3.569 ± 0.37
5.224SerLeu: 5.224 ± 0.489
1.508SerMet: 1.508 ± 0.234
2.281SerAsn: 2.281 ± 0.307
2.097SerPro: 2.097 ± 0.341
2.134SerGln: 2.134 ± 0.3
2.759SerArg: 2.759 ± 0.321
2.87SerSer: 2.87 ± 0.502
3.09SerThr: 3.09 ± 0.352
3.752SerVal: 3.752 ± 0.452
0.625SerTrp: 0.625 ± 0.152
1.692SerTyr: 1.692 ± 0.22
0.0SerXaa: 0.0 ± 0.0
Thr
6.401ThrAla: 6.401 ± 0.623
0.662ThrCys: 0.662 ± 0.175
3.973ThrAsp: 3.973 ± 0.393
3.716ThrGlu: 3.716 ± 0.422
2.207ThrPhe: 2.207 ± 0.3
5.371ThrGly: 5.371 ± 0.559
1.14ThrHis: 1.14 ± 0.261
3.716ThrIle: 3.716 ± 0.46
3.716ThrLys: 3.716 ± 0.427
6.217ThrLeu: 6.217 ± 0.545
1.398ThrMet: 1.398 ± 0.254
2.686ThrAsn: 2.686 ± 0.313
3.421ThrPro: 3.421 ± 0.369
2.244ThrGln: 2.244 ± 0.298
3.053ThrArg: 3.053 ± 0.351
3.09ThrSer: 3.09 ± 0.305
4.819ThrThr: 4.819 ± 0.643
4.157ThrVal: 4.157 ± 0.435
0.773ThrTrp: 0.773 ± 0.164
1.619ThrTyr: 1.619 ± 0.251
0.0ThrXaa: 0.0 ± 0.0
Val
6.401ValAla: 6.401 ± 0.515
0.736ValCys: 0.736 ± 0.184
5.114ValAsp: 5.114 ± 0.403
4.268ValGlu: 4.268 ± 0.455
1.876ValPhe: 1.876 ± 0.209
5.555ValGly: 5.555 ± 0.369
1.288ValHis: 1.288 ± 0.231
3.127ValIle: 3.127 ± 0.327
4.709ValLys: 4.709 ± 0.443
5.886ValLeu: 5.886 ± 0.461
2.428ValMet: 2.428 ± 0.285
2.538ValAsn: 2.538 ± 0.295
3.458ValPro: 3.458 ± 0.364
2.207ValGln: 2.207 ± 0.275
3.495ValArg: 3.495 ± 0.365
3.826ValSer: 3.826 ± 0.322
4.562ValThr: 4.562 ± 0.609
5.077ValVal: 5.077 ± 0.473
1.177ValTrp: 1.177 ± 0.251
2.281ValTyr: 2.281 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
0.957TrpAla: 0.957 ± 0.203
0.258TrpCys: 0.258 ± 0.112
1.104TrpAsp: 1.104 ± 0.21
1.067TrpGlu: 1.067 ± 0.209
0.331TrpPhe: 0.331 ± 0.103
0.846TrpGly: 0.846 ± 0.166
0.184TrpHis: 0.184 ± 0.073
0.625TrpIle: 0.625 ± 0.151
1.03TrpLys: 1.03 ± 0.164
1.251TrpLeu: 1.251 ± 0.201
0.331TrpMet: 0.331 ± 0.106
0.625TrpAsn: 0.625 ± 0.137
0.552TrpPro: 0.552 ± 0.155
0.625TrpGln: 0.625 ± 0.182
1.104TrpArg: 1.104 ± 0.233
0.736TrpSer: 0.736 ± 0.193
0.773TrpThr: 0.773 ± 0.193
1.288TrpVal: 1.288 ± 0.223
0.294TrpTrp: 0.294 ± 0.121
0.552TrpTyr: 0.552 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.354TyrAla: 2.354 ± 0.299
0.662TyrCys: 0.662 ± 0.159
1.913TyrAsp: 1.913 ± 0.236
1.876TyrGlu: 1.876 ± 0.314
1.03TyrPhe: 1.03 ± 0.198
2.281TyrGly: 2.281 ± 0.276
0.589TyrHis: 0.589 ± 0.135
1.435TyrIle: 1.435 ± 0.203
1.472TyrLys: 1.472 ± 0.206
1.913TyrLeu: 1.913 ± 0.263
0.883TyrMet: 0.883 ± 0.208
1.582TyrAsn: 1.582 ± 0.241
1.508TyrPro: 1.508 ± 0.269
0.957TyrGln: 0.957 ± 0.163
2.097TyrArg: 2.097 ± 0.265
1.582TyrSer: 1.582 ± 0.203
2.354TyrThr: 2.354 ± 0.374
2.171TyrVal: 2.171 ± 0.238
0.773TyrTrp: 0.773 ± 0.166
1.324TyrTyr: 1.324 ± 0.245
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 116 proteins (27183 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski