Amino acid dipepetide frequency for Gordonia phage JuJu

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.509AlaAla: 17.509 ± 1.318
0.629AlaCys: 0.629 ± 0.203
8.125AlaAsp: 8.125 ± 0.754
7.724AlaGlu: 7.724 ± 0.83
3.433AlaPhe: 3.433 ± 0.592
8.64AlaGly: 8.64 ± 0.947
2.518AlaHis: 2.518 ± 0.331
4.864AlaIle: 4.864 ± 0.484
3.948AlaLys: 3.948 ± 0.697
10.185AlaLeu: 10.185 ± 0.909
2.861AlaMet: 2.861 ± 0.527
3.09AlaAsn: 3.09 ± 0.54
4.864AlaPro: 4.864 ± 0.519
4.577AlaGln: 4.577 ± 0.525
8.869AlaArg: 8.869 ± 0.772
5.092AlaSer: 5.092 ± 0.571
6.408AlaThr: 6.408 ± 0.62
7.496AlaVal: 7.496 ± 0.878
2.06AlaTrp: 2.06 ± 0.341
1.659AlaTyr: 1.659 ± 0.329
0.0AlaXaa: 0.0 ± 0.0
Cys
0.687CysAla: 0.687 ± 0.206
0.229CysCys: 0.229 ± 0.143
0.973CysAsp: 0.973 ± 0.326
0.401CysGlu: 0.401 ± 0.224
0.057CysPhe: 0.057 ± 0.062
1.373CysGly: 1.373 ± 0.4
0.629CysHis: 0.629 ± 0.202
0.114CysIle: 0.114 ± 0.077
0.172CysLys: 0.172 ± 0.106
0.458CysLeu: 0.458 ± 0.156
0.172CysMet: 0.172 ± 0.096
0.515CysAsn: 0.515 ± 0.183
0.858CysPro: 0.858 ± 0.214
0.172CysGln: 0.172 ± 0.1
0.801CysArg: 0.801 ± 0.276
0.515CysSer: 0.515 ± 0.185
0.572CysThr: 0.572 ± 0.17
0.572CysVal: 0.572 ± 0.178
0.229CysTrp: 0.229 ± 0.135
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
7.724AspAla: 7.724 ± 0.781
0.286AspCys: 0.286 ± 0.126
6.523AspAsp: 6.523 ± 1.06
4.864AspGlu: 4.864 ± 0.775
1.659AspPhe: 1.659 ± 0.298
6.695AspGly: 6.695 ± 0.753
1.717AspHis: 1.717 ± 0.359
3.261AspIle: 3.261 ± 0.439
1.888AspLys: 1.888 ± 0.321
5.55AspLeu: 5.55 ± 0.597
1.373AspMet: 1.373 ± 0.24
2.06AspAsn: 2.06 ± 0.368
4.577AspPro: 4.577 ± 0.565
2.861AspGln: 2.861 ± 0.391
4.577AspArg: 4.577 ± 0.669
3.261AspSer: 3.261 ± 0.383
4.005AspThr: 4.005 ± 0.529
5.493AspVal: 5.493 ± 0.61
1.259AspTrp: 1.259 ± 0.28
1.717AspTyr: 1.717 ± 0.351
0.0AspXaa: 0.0 ± 0.0
Glu
6.58GluAla: 6.58 ± 0.82
0.572GluCys: 0.572 ± 0.204
2.746GluAsp: 2.746 ± 0.4
2.918GluGlu: 2.918 ± 0.569
2.117GluPhe: 2.117 ± 0.388
4.062GluGly: 4.062 ± 0.429
1.316GluHis: 1.316 ± 0.229
2.689GluIle: 2.689 ± 0.349
2.232GluLys: 2.232 ± 0.323
5.436GluLeu: 5.436 ± 0.879
1.316GluMet: 1.316 ± 0.267
1.373GluAsn: 1.373 ± 0.309
3.776GluPro: 3.776 ± 0.599
3.033GluGln: 3.033 ± 0.393
5.035GluArg: 5.035 ± 0.556
2.746GluSer: 2.746 ± 0.395
3.49GluThr: 3.49 ± 0.375
5.035GluVal: 5.035 ± 0.516
1.144GluTrp: 1.144 ± 0.305
1.659GluTyr: 1.659 ± 0.332
0.0GluXaa: 0.0 ± 0.0
Phe
2.918PheAla: 2.918 ± 0.341
0.458PheCys: 0.458 ± 0.171
1.945PheAsp: 1.945 ± 0.283
1.717PheGlu: 1.717 ± 0.346
0.973PhePhe: 0.973 ± 0.265
2.518PheGly: 2.518 ± 0.373
0.458PheHis: 0.458 ± 0.188
0.973PheIle: 0.973 ± 0.288
0.915PheLys: 0.915 ± 0.288
1.488PheLeu: 1.488 ± 0.377
0.744PheMet: 0.744 ± 0.196
0.858PheAsn: 0.858 ± 0.229
1.43PhePro: 1.43 ± 0.244
0.744PheGln: 0.744 ± 0.19
2.346PheArg: 2.346 ± 0.327
1.144PheSer: 1.144 ± 0.263
2.403PheThr: 2.403 ± 0.401
2.346PheVal: 2.346 ± 0.349
0.458PheTrp: 0.458 ± 0.182
0.629PheTyr: 0.629 ± 0.17
0.0PheXaa: 0.0 ± 0.0
Gly
7.953GlyAla: 7.953 ± 0.987
0.515GlyCys: 0.515 ± 0.185
6.065GlyAsp: 6.065 ± 0.576
5.035GlyGlu: 5.035 ± 0.667
2.804GlyPhe: 2.804 ± 0.402
7.209GlyGly: 7.209 ± 0.892
1.488GlyHis: 1.488 ± 0.291
3.776GlyIle: 3.776 ± 0.673
3.376GlyLys: 3.376 ± 0.534
7.095GlyLeu: 7.095 ± 1.051
1.43GlyMet: 1.43 ± 0.247
2.975GlyAsn: 2.975 ± 0.363
4.005GlyPro: 4.005 ± 0.524
2.861GlyGln: 2.861 ± 0.427
6.58GlyArg: 6.58 ± 0.663
3.49GlySer: 3.49 ± 0.478
4.806GlyThr: 4.806 ± 0.512
6.065GlyVal: 6.065 ± 0.537
2.06GlyTrp: 2.06 ± 0.392
2.689GlyTyr: 2.689 ± 0.286
0.0GlyXaa: 0.0 ± 0.0
His
2.232HisAla: 2.232 ± 0.309
0.114HisCys: 0.114 ± 0.085
1.888HisAsp: 1.888 ± 0.311
1.087HisGlu: 1.087 ± 0.276
0.687HisPhe: 0.687 ± 0.191
1.774HisGly: 1.774 ± 0.327
0.687HisHis: 0.687 ± 0.28
0.973HisIle: 0.973 ± 0.232
0.343HisLys: 0.343 ± 0.134
2.117HisLeu: 2.117 ± 0.272
0.343HisMet: 0.343 ± 0.124
0.401HisAsn: 0.401 ± 0.157
2.003HisPro: 2.003 ± 0.366
1.202HisGln: 1.202 ± 0.279
2.003HisArg: 2.003 ± 0.328
1.202HisSer: 1.202 ± 0.246
1.43HisThr: 1.43 ± 0.29
1.43HisVal: 1.43 ± 0.311
0.629HisTrp: 0.629 ± 0.211
0.458HisTyr: 0.458 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
5.893IleAla: 5.893 ± 0.525
0.515IleCys: 0.515 ± 0.19
4.062IleAsp: 4.062 ± 0.489
2.918IleGlu: 2.918 ± 0.474
0.858IlePhe: 0.858 ± 0.253
4.749IleGly: 4.749 ± 0.673
0.687IleHis: 0.687 ± 0.183
1.488IleIle: 1.488 ± 0.259
1.43IleLys: 1.43 ± 0.468
1.945IleLeu: 1.945 ± 0.325
0.286IleMet: 0.286 ± 0.124
1.488IleAsn: 1.488 ± 0.317
2.861IlePro: 2.861 ± 0.478
0.973IleGln: 0.973 ± 0.21
3.662IleArg: 3.662 ± 0.553
1.831IleSer: 1.831 ± 0.282
3.548IleThr: 3.548 ± 0.463
3.834IleVal: 3.834 ± 0.467
0.286IleTrp: 0.286 ± 0.148
0.915IleTyr: 0.915 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
3.319LysAla: 3.319 ± 0.41
0.172LysCys: 0.172 ± 0.092
1.945LysAsp: 1.945 ± 0.374
1.087LysGlu: 1.087 ± 0.257
0.973LysPhe: 0.973 ± 0.253
2.975LysGly: 2.975 ± 0.48
0.286LysHis: 0.286 ± 0.14
1.831LysIle: 1.831 ± 0.376
1.945LysLys: 1.945 ± 0.359
2.861LysLeu: 2.861 ± 0.411
0.915LysMet: 0.915 ± 0.296
1.03LysAsn: 1.03 ± 0.245
2.518LysPro: 2.518 ± 0.451
1.144LysGln: 1.144 ± 0.194
2.232LysArg: 2.232 ± 0.385
1.659LysSer: 1.659 ± 0.378
2.746LysThr: 2.746 ± 0.381
2.232LysVal: 2.232 ± 0.326
0.915LysTrp: 0.915 ± 0.236
0.687LysTyr: 0.687 ± 0.205
0.0LysXaa: 0.0 ± 0.0
Leu
9.842LeuAla: 9.842 ± 0.964
0.744LeuCys: 0.744 ± 0.241
5.321LeuAsp: 5.321 ± 0.711
4.062LeuGlu: 4.062 ± 0.681
2.117LeuPhe: 2.117 ± 0.263
6.18LeuGly: 6.18 ± 0.701
1.144LeuHis: 1.144 ± 0.302
3.433LeuIle: 3.433 ± 0.49
1.602LeuLys: 1.602 ± 0.315
4.864LeuLeu: 4.864 ± 0.587
2.003LeuMet: 2.003 ± 0.315
2.117LeuAsn: 2.117 ± 0.416
4.005LeuPro: 4.005 ± 0.46
1.717LeuGln: 1.717 ± 0.312
5.665LeuArg: 5.665 ± 0.679
4.921LeuSer: 4.921 ± 0.532
5.436LeuThr: 5.436 ± 0.575
6.923LeuVal: 6.923 ± 0.633
1.888LeuTrp: 1.888 ± 0.401
1.488LeuTyr: 1.488 ± 0.279
0.0LeuXaa: 0.0 ± 0.0
Met
3.548MetAla: 3.548 ± 0.582
0.286MetCys: 0.286 ± 0.122
0.744MetAsp: 0.744 ± 0.175
0.744MetGlu: 0.744 ± 0.185
0.515MetPhe: 0.515 ± 0.172
1.602MetGly: 1.602 ± 0.359
0.401MetHis: 0.401 ± 0.147
0.915MetIle: 0.915 ± 0.23
0.858MetLys: 0.858 ± 0.218
1.316MetLeu: 1.316 ± 0.317
0.515MetMet: 0.515 ± 0.177
0.629MetAsn: 0.629 ± 0.183
1.945MetPro: 1.945 ± 0.29
0.687MetGln: 0.687 ± 0.25
2.174MetArg: 2.174 ± 0.436
1.488MetSer: 1.488 ± 0.286
2.46MetThr: 2.46 ± 0.319
0.629MetVal: 0.629 ± 0.209
0.458MetTrp: 0.458 ± 0.181
0.343MetTyr: 0.343 ± 0.136
0.0MetXaa: 0.0 ± 0.0
Asn
3.376AsnAla: 3.376 ± 0.458
0.114AsnCys: 0.114 ± 0.086
1.659AsnAsp: 1.659 ± 0.25
0.973AsnGlu: 0.973 ± 0.216
0.515AsnPhe: 0.515 ± 0.2
2.804AsnGly: 2.804 ± 0.41
1.259AsnHis: 1.259 ± 0.398
1.03AsnIle: 1.03 ± 0.279
0.572AsnLys: 0.572 ± 0.182
2.518AsnLeu: 2.518 ± 0.383
0.458AsnMet: 0.458 ± 0.149
1.03AsnAsn: 1.03 ± 0.272
3.147AsnPro: 3.147 ± 0.46
1.202AsnGln: 1.202 ± 0.261
1.774AsnArg: 1.774 ± 0.351
2.117AsnSer: 2.117 ± 0.347
1.945AsnThr: 1.945 ± 0.469
2.06AsnVal: 2.06 ± 0.383
0.343AsnTrp: 0.343 ± 0.124
0.801AsnTyr: 0.801 ± 0.223
0.0AsnXaa: 0.0 ± 0.0
Pro
6.008ProAla: 6.008 ± 0.689
0.801ProCys: 0.801 ± 0.219
4.635ProAsp: 4.635 ± 0.583
4.177ProGlu: 4.177 ± 0.54
1.602ProPhe: 1.602 ± 0.295
4.806ProGly: 4.806 ± 0.556
1.659ProHis: 1.659 ± 0.327
2.918ProIle: 2.918 ± 0.35
2.46ProLys: 2.46 ± 0.371
3.376ProLeu: 3.376 ± 0.446
1.488ProMet: 1.488 ± 0.414
2.289ProAsn: 2.289 ± 0.377
2.632ProPro: 2.632 ± 0.458
1.888ProGln: 1.888 ± 0.328
3.261ProArg: 3.261 ± 0.556
3.261ProSer: 3.261 ± 0.454
4.062ProThr: 4.062 ± 0.539
3.433ProVal: 3.433 ± 0.449
1.373ProTrp: 1.373 ± 0.298
1.144ProTyr: 1.144 ± 0.273
0.0ProXaa: 0.0 ± 0.0
Gln
3.834GlnAla: 3.834 ± 0.554
0.172GlnCys: 0.172 ± 0.102
1.43GlnAsp: 1.43 ± 0.372
1.087GlnGlu: 1.087 ± 0.287
1.087GlnPhe: 1.087 ± 0.257
2.346GlnGly: 2.346 ± 0.347
1.259GlnHis: 1.259 ± 0.307
1.602GlnIle: 1.602 ± 0.285
1.03GlnLys: 1.03 ± 0.22
3.261GlnLeu: 3.261 ± 0.398
0.858GlnMet: 0.858 ± 0.245
0.687GlnAsn: 0.687 ± 0.176
2.518GlnPro: 2.518 ± 0.464
1.888GlnGln: 1.888 ± 0.391
3.09GlnArg: 3.09 ± 0.413
2.232GlnSer: 2.232 ± 0.361
1.774GlnThr: 1.774 ± 0.32
2.975GlnVal: 2.975 ± 0.412
0.973GlnTrp: 0.973 ± 0.22
0.973GlnTyr: 0.973 ± 0.228
0.0GlnXaa: 0.0 ± 0.0
Arg
7.896ArgAla: 7.896 ± 0.584
0.915ArgCys: 0.915 ± 0.277
6.122ArgAsp: 6.122 ± 0.555
4.806ArgGlu: 4.806 ± 0.692
1.888ArgPhe: 1.888 ± 0.409
6.294ArgGly: 6.294 ± 0.577
2.289ArgHis: 2.289 ± 0.348
3.891ArgIle: 3.891 ± 0.511
2.518ArgLys: 2.518 ± 0.255
5.722ArgLeu: 5.722 ± 0.645
2.289ArgMet: 2.289 ± 0.369
2.746ArgAsn: 2.746 ± 0.331
3.261ArgPro: 3.261 ± 0.514
2.575ArgGln: 2.575 ± 0.386
7.782ArgArg: 7.782 ± 1.052
4.349ArgSer: 4.349 ± 0.502
3.776ArgThr: 3.776 ± 0.385
5.436ArgVal: 5.436 ± 0.643
1.945ArgTrp: 1.945 ± 0.425
1.774ArgTyr: 1.774 ± 0.33
0.0ArgXaa: 0.0 ± 0.0
Ser
5.779SerAla: 5.779 ± 0.75
0.229SerCys: 0.229 ± 0.106
3.433SerAsp: 3.433 ± 0.519
3.261SerGlu: 3.261 ± 0.435
1.259SerPhe: 1.259 ± 0.265
4.635SerGly: 4.635 ± 0.613
1.202SerHis: 1.202 ± 0.303
2.518SerIle: 2.518 ± 0.379
1.488SerLys: 1.488 ± 0.356
3.033SerLeu: 3.033 ± 0.449
1.259SerMet: 1.259 ± 0.207
1.488SerAsn: 1.488 ± 0.349
3.033SerPro: 3.033 ± 0.412
1.545SerGln: 1.545 ± 0.339
3.662SerArg: 3.662 ± 0.451
2.232SerSer: 2.232 ± 0.317
4.177SerThr: 4.177 ± 0.471
4.291SerVal: 4.291 ± 0.595
1.373SerTrp: 1.373 ± 0.214
0.858SerTyr: 0.858 ± 0.232
0.0SerXaa: 0.0 ± 0.0
Thr
7.724ThrAla: 7.724 ± 0.657
0.858ThrCys: 0.858 ± 0.233
5.207ThrAsp: 5.207 ± 0.605
4.291ThrGlu: 4.291 ± 0.525
1.888ThrPhe: 1.888 ± 0.423
5.207ThrGly: 5.207 ± 0.632
1.831ThrHis: 1.831 ± 0.358
2.918ThrIle: 2.918 ± 0.364
2.746ThrLys: 2.746 ± 0.441
5.035ThrLeu: 5.035 ± 0.58
1.259ThrMet: 1.259 ± 0.273
1.774ThrAsn: 1.774 ± 0.314
3.605ThrPro: 3.605 ± 0.367
2.575ThrGln: 2.575 ± 0.362
4.463ThrArg: 4.463 ± 0.511
2.746ThrSer: 2.746 ± 0.387
4.978ThrThr: 4.978 ± 0.568
5.321ThrVal: 5.321 ± 0.456
1.03ThrTrp: 1.03 ± 0.276
1.43ThrTyr: 1.43 ± 0.358
0.0ThrXaa: 0.0 ± 0.0
Val
8.354ValAla: 8.354 ± 0.775
1.259ValCys: 1.259 ± 0.315
6.18ValAsp: 6.18 ± 0.635
5.207ValGlu: 5.207 ± 0.509
1.717ValPhe: 1.717 ± 0.273
5.607ValGly: 5.607 ± 0.627
1.087ValHis: 1.087 ± 0.218
3.376ValIle: 3.376 ± 0.423
2.689ValLys: 2.689 ± 0.371
5.607ValLeu: 5.607 ± 0.695
1.774ValMet: 1.774 ± 0.308
1.831ValAsn: 1.831 ± 0.301
3.719ValPro: 3.719 ± 0.42
1.888ValGln: 1.888 ± 0.344
6.466ValArg: 6.466 ± 0.644
3.776ValSer: 3.776 ± 0.558
5.493ValThr: 5.493 ± 0.62
6.981ValVal: 6.981 ± 0.751
1.717ValTrp: 1.717 ± 0.358
1.545ValTyr: 1.545 ± 0.265
0.0ValXaa: 0.0 ± 0.0
Trp
1.488TrpAla: 1.488 ± 0.292
0.343TrpCys: 0.343 ± 0.14
1.087TrpAsp: 1.087 ± 0.248
1.316TrpGlu: 1.316 ± 0.3
0.629TrpPhe: 0.629 ± 0.215
1.087TrpGly: 1.087 ± 0.309
0.458TrpHis: 0.458 ± 0.147
0.687TrpIle: 0.687 ± 0.17
0.744TrpLys: 0.744 ± 0.207
2.003TrpLeu: 2.003 ± 0.294
0.515TrpMet: 0.515 ± 0.166
0.744TrpAsn: 0.744 ± 0.312
1.545TrpPro: 1.545 ± 0.269
1.03TrpGln: 1.03 ± 0.23
1.945TrpArg: 1.945 ± 0.288
1.259TrpSer: 1.259 ± 0.309
1.545TrpThr: 1.545 ± 0.277
1.717TrpVal: 1.717 ± 0.339
0.401TrpTrp: 0.401 ± 0.131
0.572TrpTyr: 0.572 ± 0.202
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.945TyrAla: 1.945 ± 0.339
0.286TyrCys: 0.286 ± 0.129
1.259TyrAsp: 1.259 ± 0.222
1.602TyrGlu: 1.602 ± 0.314
0.687TyrPhe: 0.687 ± 0.203
1.717TyrGly: 1.717 ± 0.349
0.629TyrHis: 0.629 ± 0.194
0.973TyrIle: 0.973 ± 0.295
0.515TyrLys: 0.515 ± 0.189
1.602TyrLeu: 1.602 ± 0.384
0.343TyrMet: 0.343 ± 0.124
0.572TyrAsn: 0.572 ± 0.132
0.973TyrPro: 0.973 ± 0.227
0.515TyrGln: 0.515 ± 0.177
1.888TyrArg: 1.888 ± 0.408
1.43TyrSer: 1.43 ± 0.326
1.831TyrThr: 1.831 ± 0.279
2.003TyrVal: 2.003 ± 0.339
0.572TyrTrp: 0.572 ± 0.164
0.458TyrTyr: 0.458 ± 0.181
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (17478 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski