Amino acid dipepetide frequency for Mycobacterium phage Ramen

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.564AlaAla: 20.564 ± 2.321
1.289AlaCys: 1.289 ± 0.265
8.215AlaAsp: 8.215 ± 0.779
9.128AlaGlu: 9.128 ± 0.775
3.651AlaPhe: 3.651 ± 0.665
9.342AlaGly: 9.342 ± 0.932
3.114AlaHis: 3.114 ± 0.369
5.477AlaIle: 5.477 ± 0.455
4.242AlaLys: 4.242 ± 0.508
11.06AlaLeu: 11.06 ± 0.779
3.49AlaMet: 3.49 ± 0.388
3.329AlaAsn: 3.329 ± 0.493
7.034AlaPro: 7.034 ± 0.732
5.047AlaGln: 5.047 ± 0.598
8.859AlaArg: 8.859 ± 0.687
4.993AlaSer: 4.993 ± 0.63
7.732AlaThr: 7.732 ± 0.857
9.987AlaVal: 9.987 ± 0.713
1.987AlaTrp: 1.987 ± 0.271
2.416AlaTyr: 2.416 ± 0.429
0.0AlaXaa: 0.0 ± 0.0
Cys
1.02CysAla: 1.02 ± 0.275
0.161CysCys: 0.161 ± 0.098
0.698CysAsp: 0.698 ± 0.241
0.698CysGlu: 0.698 ± 0.182
0.268CysPhe: 0.268 ± 0.109
1.718CysGly: 1.718 ± 0.397
0.161CysHis: 0.161 ± 0.097
0.322CysIle: 0.322 ± 0.128
0.161CysLys: 0.161 ± 0.082
0.859CysLeu: 0.859 ± 0.257
0.107CysMet: 0.107 ± 0.071
0.322CysAsn: 0.322 ± 0.145
0.913CysPro: 0.913 ± 0.318
0.376CysGln: 0.376 ± 0.132
0.698CysArg: 0.698 ± 0.229
0.698CysSer: 0.698 ± 0.217
0.644CysThr: 0.644 ± 0.183
0.859CysVal: 0.859 ± 0.242
0.322CysTrp: 0.322 ± 0.116
0.215CysTyr: 0.215 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
7.785AspAla: 7.785 ± 0.724
0.752AspCys: 0.752 ± 0.26
5.799AspAsp: 5.799 ± 0.724
5.315AspGlu: 5.315 ± 0.601
1.557AspPhe: 1.557 ± 0.28
6.765AspGly: 6.765 ± 0.536
0.913AspHis: 0.913 ± 0.234
1.557AspIle: 1.557 ± 0.295
2.201AspLys: 2.201 ± 0.335
6.228AspLeu: 6.228 ± 0.63
1.557AspMet: 1.557 ± 0.354
2.094AspAsn: 2.094 ± 0.312
4.027AspPro: 4.027 ± 0.556
2.201AspGln: 2.201 ± 0.361
4.081AspArg: 4.081 ± 0.497
2.523AspSer: 2.523 ± 0.278
3.168AspThr: 3.168 ± 0.345
4.617AspVal: 4.617 ± 0.452
1.235AspTrp: 1.235 ± 0.253
1.45AspTyr: 1.45 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
8.43GluAla: 8.43 ± 0.77
1.02GluCys: 1.02 ± 0.228
2.47GluAsp: 2.47 ± 0.386
1.45GluGlu: 1.45 ± 0.309
2.255GluPhe: 2.255 ± 0.393
4.564GluGly: 4.564 ± 0.507
1.718GluHis: 1.718 ± 0.345
2.04GluIle: 2.04 ± 0.251
1.396GluLys: 1.396 ± 0.325
6.228GluLeu: 6.228 ± 0.639
1.503GluMet: 1.503 ± 0.223
0.913GluAsn: 0.913 ± 0.219
2.792GluPro: 2.792 ± 0.454
2.792GluGln: 2.792 ± 0.372
5.047GluArg: 5.047 ± 0.648
2.362GluSer: 2.362 ± 0.338
2.792GluThr: 2.792 ± 0.298
5.315GluVal: 5.315 ± 0.847
0.966GluTrp: 0.966 ± 0.207
1.987GluTyr: 1.987 ± 0.301
0.0GluXaa: 0.0 ± 0.0
Phe
2.416PheAla: 2.416 ± 0.371
0.268PheCys: 0.268 ± 0.104
3.275PheAsp: 3.275 ± 0.44
1.45PheGlu: 1.45 ± 0.244
0.537PhePhe: 0.537 ± 0.175
2.685PheGly: 2.685 ± 0.408
0.161PheHis: 0.161 ± 0.095
1.289PheIle: 1.289 ± 0.262
1.342PheLys: 1.342 ± 0.27
2.04PheLeu: 2.04 ± 0.363
0.752PheMet: 0.752 ± 0.211
1.181PheAsn: 1.181 ± 0.361
1.181PhePro: 1.181 ± 0.223
0.644PheGln: 0.644 ± 0.182
1.557PheArg: 1.557 ± 0.317
1.074PheSer: 1.074 ± 0.209
2.04PheThr: 2.04 ± 0.394
1.718PheVal: 1.718 ± 0.31
0.43PheTrp: 0.43 ± 0.104
0.43PheTyr: 0.43 ± 0.19
0.0PheXaa: 0.0 ± 0.0
Gly
11.383GlyAla: 11.383 ± 0.957
1.181GlyCys: 1.181 ± 0.304
5.154GlyAsp: 5.154 ± 0.52
5.101GlyGlu: 5.101 ± 0.541
1.772GlyPhe: 1.772 ± 0.304
10.362GlyGly: 10.362 ± 1.417
1.557GlyHis: 1.557 ± 0.272
2.846GlyIle: 2.846 ± 0.651
4.027GlyLys: 4.027 ± 0.528
6.282GlyLeu: 6.282 ± 0.825
1.718GlyMet: 1.718 ± 0.274
2.685GlyAsn: 2.685 ± 0.331
3.919GlyPro: 3.919 ± 0.616
2.255GlyGln: 2.255 ± 0.46
6.765GlyArg: 6.765 ± 0.647
5.584GlySer: 5.584 ± 0.616
5.101GlyThr: 5.101 ± 0.497
6.819GlyVal: 6.819 ± 0.537
2.47GlyTrp: 2.47 ± 0.342
2.309GlyTyr: 2.309 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
2.309HisAla: 2.309 ± 0.378
0.322HisCys: 0.322 ± 0.125
1.235HisAsp: 1.235 ± 0.268
1.503HisGlu: 1.503 ± 0.314
0.322HisPhe: 0.322 ± 0.155
2.47HisGly: 2.47 ± 0.4
0.537HisHis: 0.537 ± 0.177
0.859HisIle: 0.859 ± 0.221
0.322HisLys: 0.322 ± 0.132
2.04HisLeu: 2.04 ± 0.283
0.215HisMet: 0.215 ± 0.101
0.591HisAsn: 0.591 ± 0.144
1.289HisPro: 1.289 ± 0.271
0.537HisGln: 0.537 ± 0.152
1.396HisArg: 1.396 ± 0.31
0.537HisSer: 0.537 ± 0.172
1.45HisThr: 1.45 ± 0.264
1.987HisVal: 1.987 ± 0.38
0.591HisTrp: 0.591 ± 0.187
0.805HisTyr: 0.805 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
5.584IleAla: 5.584 ± 0.566
0.268IleCys: 0.268 ± 0.145
3.597IleAsp: 3.597 ± 0.451
2.953IleGlu: 2.953 ± 0.348
0.859IlePhe: 0.859 ± 0.227
3.597IleGly: 3.597 ± 0.63
0.591IleHis: 0.591 ± 0.188
0.752IleIle: 0.752 ± 0.187
1.45IleLys: 1.45 ± 0.286
2.685IleLeu: 2.685 ± 0.418
0.215IleMet: 0.215 ± 0.109
1.45IleAsn: 1.45 ± 0.27
1.933IlePro: 1.933 ± 0.422
0.859IleGln: 0.859 ± 0.209
2.309IleArg: 2.309 ± 0.279
1.987IleSer: 1.987 ± 0.276
2.309IleThr: 2.309 ± 0.288
3.114IleVal: 3.114 ± 0.406
0.483IleTrp: 0.483 ± 0.169
0.483IleTyr: 0.483 ± 0.142
0.0IleXaa: 0.0 ± 0.0
Lys
4.295LysAla: 4.295 ± 0.522
0.43LysCys: 0.43 ± 0.137
1.342LysAsp: 1.342 ± 0.229
0.859LysGlu: 0.859 ± 0.178
1.342LysPhe: 1.342 ± 0.327
3.168LysGly: 3.168 ± 0.528
0.644LysHis: 0.644 ± 0.18
1.611LysIle: 1.611 ± 0.306
0.913LysLys: 0.913 ± 0.224
3.705LysLeu: 3.705 ± 0.429
0.805LysMet: 0.805 ± 0.214
0.43LysAsn: 0.43 ± 0.137
2.899LysPro: 2.899 ± 0.367
0.752LysGln: 0.752 ± 0.194
3.329LysArg: 3.329 ± 0.439
1.074LysSer: 1.074 ± 0.224
2.094LysThr: 2.094 ± 0.364
3.329LysVal: 3.329 ± 0.512
0.537LysTrp: 0.537 ± 0.162
0.752LysTyr: 0.752 ± 0.244
0.0LysXaa: 0.0 ± 0.0
Leu
11.812LeuAla: 11.812 ± 0.963
0.805LeuCys: 0.805 ± 0.282
7.302LeuAsp: 7.302 ± 0.489
2.846LeuGlu: 2.846 ± 0.43
2.04LeuPhe: 2.04 ± 0.313
6.711LeuGly: 6.711 ± 0.633
2.094LeuHis: 2.094 ± 0.36
3.49LeuIle: 3.49 ± 0.448
2.631LeuLys: 2.631 ± 0.339
5.477LeuLeu: 5.477 ± 0.477
1.826LeuMet: 1.826 ± 0.302
2.201LeuAsn: 2.201 ± 0.334
4.456LeuPro: 4.456 ± 0.415
2.846LeuGln: 2.846 ± 0.522
6.336LeuArg: 6.336 ± 0.657
5.208LeuSer: 5.208 ± 0.388
4.993LeuThr: 4.993 ± 0.593
6.336LeuVal: 6.336 ± 0.615
1.557LeuTrp: 1.557 ± 0.304
1.396LeuTyr: 1.396 ± 0.334
0.0LeuXaa: 0.0 ± 0.0
Met
2.577MetAla: 2.577 ± 0.315
0.0MetCys: 0.0 ± 0.0
0.859MetAsp: 0.859 ± 0.168
0.752MetGlu: 0.752 ± 0.181
0.752MetPhe: 0.752 ± 0.223
1.45MetGly: 1.45 ± 0.282
0.644MetHis: 0.644 ± 0.17
0.966MetIle: 0.966 ± 0.289
0.537MetLys: 0.537 ± 0.169
1.879MetLeu: 1.879 ± 0.322
0.322MetMet: 0.322 ± 0.14
0.591MetAsn: 0.591 ± 0.168
1.289MetPro: 1.289 ± 0.253
0.752MetGln: 0.752 ± 0.18
1.02MetArg: 1.02 ± 0.26
2.899MetSer: 2.899 ± 0.308
2.04MetThr: 2.04 ± 0.365
1.342MetVal: 1.342 ± 0.243
0.322MetTrp: 0.322 ± 0.12
0.483MetTyr: 0.483 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
3.544AsnAla: 3.544 ± 0.539
0.376AsnCys: 0.376 ± 0.126
1.289AsnAsp: 1.289 ± 0.301
1.664AsnGlu: 1.664 ± 0.333
0.591AsnPhe: 0.591 ± 0.146
3.275AsnGly: 3.275 ± 0.448
0.43AsnHis: 0.43 ± 0.154
0.966AsnIle: 0.966 ± 0.231
1.02AsnLys: 1.02 ± 0.255
2.309AsnLeu: 2.309 ± 0.423
0.537AsnMet: 0.537 ± 0.142
0.591AsnAsn: 0.591 ± 0.193
2.47AsnPro: 2.47 ± 0.307
0.752AsnGln: 0.752 ± 0.217
1.503AsnArg: 1.503 ± 0.296
1.235AsnSer: 1.235 ± 0.322
1.611AsnThr: 1.611 ± 0.275
2.04AsnVal: 2.04 ± 0.287
0.43AsnTrp: 0.43 ± 0.15
0.644AsnTyr: 0.644 ± 0.174
0.0AsnXaa: 0.0 ± 0.0
Pro
8.268ProAla: 8.268 ± 0.576
0.322ProCys: 0.322 ± 0.131
3.597ProAsp: 3.597 ± 0.458
4.295ProGlu: 4.295 ± 0.66
1.181ProPhe: 1.181 ± 0.226
5.799ProGly: 5.799 ± 0.613
1.074ProHis: 1.074 ± 0.332
1.772ProIle: 1.772 ± 0.246
2.362ProLys: 2.362 ± 0.349
3.812ProLeu: 3.812 ± 0.546
1.45ProMet: 1.45 ± 0.265
1.128ProAsn: 1.128 ± 0.335
2.953ProPro: 2.953 ± 0.374
1.503ProGln: 1.503 ± 0.32
3.651ProArg: 3.651 ± 0.55
3.275ProSer: 3.275 ± 0.56
3.436ProThr: 3.436 ± 0.417
4.617ProVal: 4.617 ± 0.466
1.128ProTrp: 1.128 ± 0.227
1.396ProTyr: 1.396 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
5.584GlnAla: 5.584 ± 0.567
0.376GlnCys: 0.376 ± 0.158
1.128GlnAsp: 1.128 ± 0.198
1.45GlnGlu: 1.45 ± 0.247
0.805GlnPhe: 0.805 ± 0.184
2.094GlnGly: 2.094 ± 0.309
0.966GlnHis: 0.966 ± 0.203
1.772GlnIle: 1.772 ± 0.254
1.181GlnLys: 1.181 ± 0.239
2.792GlnLeu: 2.792 ± 0.418
1.181GlnMet: 1.181 ± 0.229
0.698GlnAsn: 0.698 ± 0.226
1.933GlnPro: 1.933 ± 0.352
1.342GlnGln: 1.342 ± 0.217
2.577GlnArg: 2.577 ± 0.337
1.074GlnSer: 1.074 ± 0.232
1.772GlnThr: 1.772 ± 0.268
3.168GlnVal: 3.168 ± 0.462
0.859GlnTrp: 0.859 ± 0.226
1.074GlnTyr: 1.074 ± 0.2
0.0GlnXaa: 0.0 ± 0.0
Arg
7.087ArgAla: 7.087 ± 0.752
1.074ArgCys: 1.074 ± 0.268
4.349ArgAsp: 4.349 ± 0.639
4.779ArgGlu: 4.779 ± 0.652
2.094ArgPhe: 2.094 ± 0.347
4.993ArgGly: 4.993 ± 0.51
1.503ArgHis: 1.503 ± 0.32
2.899ArgIle: 2.899 ± 0.423
3.275ArgLys: 3.275 ± 0.358
6.121ArgLeu: 6.121 ± 0.666
1.772ArgMet: 1.772 ± 0.27
2.47ArgAsn: 2.47 ± 0.419
3.383ArgPro: 3.383 ± 0.493
3.597ArgGln: 3.597 ± 0.497
6.336ArgArg: 6.336 ± 0.819
3.114ArgSer: 3.114 ± 0.444
4.027ArgThr: 4.027 ± 0.629
4.349ArgVal: 4.349 ± 0.527
1.879ArgTrp: 1.879 ± 0.304
2.04ArgTyr: 2.04 ± 0.334
0.0ArgXaa: 0.0 ± 0.0
Ser
6.926SerAla: 6.926 ± 0.782
0.268SerCys: 0.268 ± 0.126
2.685SerAsp: 2.685 ± 0.321
2.953SerGlu: 2.953 ± 0.388
1.718SerPhe: 1.718 ± 0.347
4.349SerGly: 4.349 ± 0.499
1.02SerHis: 1.02 ± 0.285
2.148SerIle: 2.148 ± 0.401
1.128SerLys: 1.128 ± 0.204
4.242SerLeu: 4.242 ± 0.428
1.235SerMet: 1.235 ± 0.216
1.664SerAsn: 1.664 ± 0.24
3.651SerPro: 3.651 ± 0.466
1.396SerGln: 1.396 ± 0.235
2.523SerArg: 2.523 ± 0.37
2.631SerSer: 2.631 ± 0.443
3.168SerThr: 3.168 ± 0.472
4.242SerVal: 4.242 ± 0.447
1.181SerTrp: 1.181 ± 0.216
0.966SerTyr: 0.966 ± 0.202
0.0SerXaa: 0.0 ± 0.0
Thr
6.926ThrAla: 6.926 ± 0.971
0.805ThrCys: 0.805 ± 0.215
3.812ThrAsp: 3.812 ± 0.465
3.436ThrGlu: 3.436 ± 0.364
1.879ThrPhe: 1.879 ± 0.313
5.262ThrGly: 5.262 ± 0.667
1.235ThrHis: 1.235 ± 0.288
2.846ThrIle: 2.846 ± 0.427
2.309ThrLys: 2.309 ± 0.366
4.081ThrLeu: 4.081 ± 0.482
0.698ThrMet: 0.698 ± 0.206
1.45ThrAsn: 1.45 ± 0.369
4.242ThrPro: 4.242 ± 0.504
1.718ThrGln: 1.718 ± 0.39
3.651ThrArg: 3.651 ± 0.542
3.544ThrSer: 3.544 ± 0.561
3.007ThrThr: 3.007 ± 0.423
5.423ThrVal: 5.423 ± 0.471
1.235ThrTrp: 1.235 ± 0.249
1.45ThrTyr: 1.45 ± 0.247
0.0ThrXaa: 0.0 ± 0.0
Val
9.987ValAla: 9.987 ± 0.946
1.074ValCys: 1.074 ± 0.279
6.174ValAsp: 6.174 ± 0.522
5.745ValGlu: 5.745 ± 0.708
1.557ValPhe: 1.557 ± 0.282
6.765ValGly: 6.765 ± 0.677
2.255ValHis: 2.255 ± 0.479
2.631ValIle: 2.631 ± 0.347
2.738ValLys: 2.738 ± 0.39
5.745ValLeu: 5.745 ± 0.598
1.289ValMet: 1.289 ± 0.274
1.987ValAsn: 1.987 ± 0.385
4.94ValPro: 4.94 ± 0.524
2.094ValGln: 2.094 ± 0.315
5.369ValArg: 5.369 ± 0.528
3.973ValSer: 3.973 ± 0.459
4.564ValThr: 4.564 ± 0.491
6.067ValVal: 6.067 ± 0.695
2.523ValTrp: 2.523 ± 0.377
1.557ValTyr: 1.557 ± 0.302
0.0ValXaa: 0.0 ± 0.0
Trp
2.04TrpAla: 2.04 ± 0.285
0.268TrpCys: 0.268 ± 0.108
1.02TrpAsp: 1.02 ± 0.288
0.752TrpGlu: 0.752 ± 0.187
0.537TrpPhe: 0.537 ± 0.17
1.181TrpGly: 1.181 ± 0.219
0.483TrpHis: 0.483 ± 0.162
0.805TrpIle: 0.805 ± 0.229
0.376TrpLys: 0.376 ± 0.122
3.007TrpLeu: 3.007 ± 0.407
0.43TrpMet: 0.43 ± 0.15
0.537TrpAsn: 0.537 ± 0.143
1.02TrpPro: 1.02 ± 0.217
1.128TrpGln: 1.128 ± 0.235
2.523TrpArg: 2.523 ± 0.396
1.181TrpSer: 1.181 ± 0.256
1.289TrpThr: 1.289 ± 0.226
1.718TrpVal: 1.718 ± 0.281
0.43TrpTrp: 0.43 ± 0.156
0.376TrpTyr: 0.376 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.416TyrAla: 2.416 ± 0.291
0.161TyrCys: 0.161 ± 0.085
1.826TyrAsp: 1.826 ± 0.365
0.913TyrGlu: 0.913 ± 0.176
0.752TyrPhe: 0.752 ± 0.192
2.738TyrGly: 2.738 ± 0.448
0.215TyrHis: 0.215 ± 0.095
0.43TyrIle: 0.43 ± 0.161
0.698TyrLys: 0.698 ± 0.216
1.826TyrLeu: 1.826 ± 0.333
0.322TyrMet: 0.322 ± 0.113
0.966TyrAsn: 0.966 ± 0.191
0.698TyrPro: 0.698 ± 0.193
1.235TyrGln: 1.235 ± 0.267
1.611TyrArg: 1.611 ± 0.291
1.128TyrSer: 1.128 ± 0.246
1.772TyrThr: 1.772 ± 0.307
1.987TyrVal: 1.987 ± 0.365
0.537TyrTrp: 0.537 ± 0.18
0.537TyrTyr: 0.537 ± 0.167
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (18626 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski