Triticum turgidum subsp. durum (Durum wheat) (Triticum durum)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade;

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 188121 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A446WTP0|A0A446WTP0_TRITD Isoform of A0A446WTP2 Uncharacterized protein OS=Triticum turgidum subsp. durum OX=4567 GN=TRITD_6Bv1G223910 PE=4 SV=1
MM1 pKa = 7.42SLHH4 pKa = 6.32NSFDD8 pKa = 2.84IMMFILLLGAQDD20 pKa = 3.29MHH22 pKa = 5.84VVCRR26 pKa = 11.84IFQKK30 pKa = 10.73VGSGPQNGAQYY41 pKa = 9.57GAPYY45 pKa = 9.48MEE47 pKa = 5.18EE48 pKa = 3.88EE49 pKa = 4.76WEE51 pKa = 4.61DD52 pKa = 3.53EE53 pKa = 4.22DD54 pKa = 5.85DD55 pKa = 5.58AIEE58 pKa = 4.09NTPTSGTSTEE68 pKa = 4.13MLAITDD74 pKa = 3.86AASAEE79 pKa = 4.5SNVEE83 pKa = 3.92DD84 pKa = 3.37EE85 pKa = 5.45HH86 pKa = 8.41IFSGINEE93 pKa = 4.56FVQTPEE99 pKa = 3.58VLIPQEE105 pKa = 4.07IAPLQAIAPLQVQDD119 pKa = 4.47FNEE122 pKa = 4.32TGMGSYY128 pKa = 11.23ADD130 pKa = 3.79GDD132 pKa = 4.02VPLNEE137 pKa = 4.3ILQEE141 pKa = 4.02PVSDD145 pKa = 3.64VSVDD149 pKa = 3.41NTGEE153 pKa = 4.08PEE155 pKa = 4.15EE156 pKa = 4.08QSPLDD161 pKa = 3.73DD162 pKa = 4.51HH163 pKa = 7.24FSLADD168 pKa = 3.78LSGCHH173 pKa = 5.82NQDD176 pKa = 3.17DD177 pKa = 5.44GYY179 pKa = 11.4VRR181 pKa = 11.84QAGHH185 pKa = 5.92TMWSDD190 pKa = 3.17PSIGDD195 pKa = 3.38QASYY199 pKa = 11.23PMRR202 pKa = 11.84TYY204 pKa = 11.48GNRR207 pKa = 11.84NHH209 pKa = 7.39ANRR212 pKa = 11.84TLSDD216 pKa = 3.57EE217 pKa = 4.33EE218 pKa = 4.9FFDD221 pKa = 4.33TGNDD225 pKa = 3.06ANAYY229 pKa = 9.91SGQQQACPSDD239 pKa = 3.66DD240 pKa = 3.52QNLYY244 pKa = 10.96SGLQQACPSDD254 pKa = 3.84EE255 pKa = 4.17QNLYY259 pKa = 9.9LQPNGLPFPQQLDD272 pKa = 3.79DD273 pKa = 3.79NAPFYY278 pKa = 7.8EE279 pKa = 4.43TSSDD283 pKa = 3.76HH284 pKa = 5.81KK285 pKa = 10.8WEE287 pKa = 4.11VGKK290 pKa = 10.36DD291 pKa = 3.4GYY293 pKa = 11.85VNVDD297 pKa = 4.24DD298 pKa = 5.36ISLFEE303 pKa = 6.42DD304 pKa = 3.98DD305 pKa = 6.57DD306 pKa = 5.12IMALLNASDD315 pKa = 5.71DD316 pKa = 4.15DD317 pKa = 4.49FSSNLLGPVDD327 pKa = 4.33GSNSQLPAASNFDD340 pKa = 3.57QKK342 pKa = 11.64DD343 pKa = 3.39EE344 pKa = 4.54AKK346 pKa = 9.97AQYY349 pKa = 9.62GASSSGSHH357 pKa = 6.17EE358 pKa = 4.16NLYY361 pKa = 9.86PDD363 pKa = 3.68TMVPDD368 pKa = 3.73VPMDD372 pKa = 4.21DD373 pKa = 3.96NAGKK377 pKa = 10.56RR378 pKa = 11.84YY379 pKa = 6.21FTNMLGSYY387 pKa = 7.79PAPPAMASEE396 pKa = 4.84FPPTTGKK403 pKa = 10.59SIAALSGPSQIRR415 pKa = 11.84VTAGIVQLGDD425 pKa = 3.25HH426 pKa = 7.34ADD428 pKa = 4.51NSDD431 pKa = 3.11HH432 pKa = 7.48WPLQKK437 pKa = 10.89NGVFNLLLSFTVEE450 pKa = 4.5SNVSTKK456 pKa = 10.71SITFDD461 pKa = 4.28DD462 pKa = 4.76EE463 pKa = 4.18PATTRR468 pKa = 11.84VSTVPTVLRR477 pKa = 11.84GGLYY481 pKa = 10.61LFFVSAMILMLSFKK495 pKa = 10.49VGSCIYY501 pKa = 10.71SRR503 pKa = 3.98

Molecular weight: 54.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A446WMR1|A0A446WMR1_TRITD WAT1-related protein OS=Triticum turgidum subsp. durum OX=4567 GN=TRITD_6Bv1G193150 PE=3 SV=1
MM1 pKa = 7.57ARR3 pKa = 11.84TKK5 pKa = 9.25QTARR9 pKa = 11.84KK10 pKa = 7.78STGGKK15 pKa = 9.94APRR18 pKa = 11.84KK19 pKa = 9.29QLATKK24 pKa = 10.05AARR27 pKa = 11.84KK28 pKa = 8.76SAPTTGGVKK37 pKa = 9.88KK38 pKa = 8.99PHH40 pKa = 5.86RR41 pKa = 11.84HH42 pKa = 5.36RR43 pKa = 11.84PGTVALRR50 pKa = 11.84LRR52 pKa = 11.84SASTSRR58 pKa = 11.84ARR60 pKa = 11.84SYY62 pKa = 11.42SSGSFHH68 pKa = 7.51SRR70 pKa = 11.84GLLGRR75 pKa = 11.84LPRR78 pKa = 11.84TSRR81 pKa = 11.84LTSASRR87 pKa = 11.84AMRR90 pKa = 11.84CWPFRR95 pKa = 11.84RR96 pKa = 11.84LQRR99 pKa = 11.84PTWWVSSRR107 pKa = 11.84IRR109 pKa = 11.84GEE111 pKa = 3.85RR112 pKa = 11.84AA113 pKa = 2.7

Molecular weight: 12.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62645

125476

188121

87439270

63

5360

464.8

51.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.309 ± 0.007

1.85 ± 0.002

5.576 ± 0.004

6.227 ± 0.006

3.744 ± 0.003

7.001 ± 0.006

2.471 ± 0.002

4.712 ± 0.005

5.344 ± 0.005

9.671 ± 0.008

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.504 ± 0.002

3.775 ± 0.004

5.243 ± 0.005

3.725 ± 0.004

5.786 ± 0.005

8.444 ± 0.006

4.883 ± 0.003

6.747 ± 0.004

1.251 ± 0.002

2.688 ± 0.003
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski