Amino acid dipepetide frequency for Clostridium liquoris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.305AlaAla: 4.305 ± 0.101	0.754AlaCys: 0.754 ± 0.033	2.604AlaAsp: 2.604 ± 0.062	3.615AlaGlu: 3.615 ± 0.072	2.583AlaPhe: 2.583 ± 0.063	3.828AlaGly: 3.828 ± 0.073	0.797AlaHis: 0.797 ± 0.037	5.827AlaIle: 5.827 ± 0.098	4.828AlaLys: 4.828 ± 0.077	6.129AlaLeu: 6.129 ± 0.085	1.753AlaMet: 1.753 ± 0.054	2.577AlaAsn: 2.577 ± 0.066	1.487AlaPro: 1.487 ± 0.042	1.421AlaGln: 1.421 ± 0.039	1.854AlaArg: 1.854 ± 0.052	3.255AlaSer: 3.255 ± 0.064	2.614AlaThr: 2.614 ± 0.066	4.222AlaVal: 4.222 ± 0.071	0.361AlaTrp: 0.361 ± 0.022	2.258AlaTyr: 2.258 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.605CysAla: 0.605 ± 0.023	0.243CysCys: 0.243 ± 0.018	0.65CysAsp: 0.65 ± 0.027	0.813CysGlu: 0.813 ± 0.032	0.487CysPhe: 0.487 ± 0.024	1.214CysGly: 1.214 ± 0.044	0.225CysHis: 0.225 ± 0.018	1.249CysIle: 1.249 ± 0.039	1.117CysLys: 1.117 ± 0.037	0.903CysLeu: 0.903 ± 0.034	0.295CysMet: 0.295 ± 0.021	0.829CysAsn: 0.829 ± 0.031	0.513CysPro: 0.513 ± 0.029	0.249CysGln: 0.249 ± 0.016	0.417CysArg: 0.417 ± 0.021	0.821CysSer: 0.821 ± 0.031	0.581CysThr: 0.581 ± 0.026	0.692CysVal: 0.692 ± 0.031	0.058CysTrp: 0.058 ± 0.008	0.512CysTyr: 0.512 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	2.616AspAla: 2.616 ± 0.056	0.607AspCys: 0.607 ± 0.029	2.323AspAsp: 2.323 ± 0.059	4.369AspGlu: 4.369 ± 0.079	2.753AspPhe: 2.753 ± 0.055	3.227AspGly: 3.227 ± 0.075	0.576AspHis: 0.576 ± 0.031	6.204AspIle: 6.204 ± 0.088	5.846AspLys: 5.846 ± 0.102	4.853AspLeu: 4.853 ± 0.075	1.649AspMet: 1.649 ± 0.039	3.374AspAsn: 3.374 ± 0.07	1.355AspPro: 1.355 ± 0.041	0.73AspGln: 0.73 ± 0.036	2.042AspArg: 2.042 ± 0.05	3.044AspSer: 3.044 ± 0.067	2.526AspThr: 2.526 ± 0.06	3.438AspVal: 3.438 ± 0.07	0.374AspTrp: 0.374 ± 0.022	2.639AspTyr: 2.639 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	4.226GluAla: 4.226 ± 0.066	0.665GluCys: 0.665 ± 0.032	4.572GluAsp: 4.572 ± 0.078	7.3GluGlu: 7.3 ± 0.126	2.824GluPhe: 2.824 ± 0.057	4.221GluGly: 4.221 ± 0.079	0.944GluHis: 0.944 ± 0.034	6.95GluIle: 6.95 ± 0.109	8.207GluLys: 8.207 ± 0.119	6.523GluLeu: 6.523 ± 0.095	1.972GluMet: 1.972 ± 0.052	5.354GluAsn: 5.354 ± 0.09	1.417GluPro: 1.417 ± 0.048	1.743GluGln: 1.743 ± 0.05	2.751GluArg: 2.751 ± 0.061	3.326GluSer: 3.326 ± 0.064	2.902GluThr: 2.902 ± 0.058	4.467GluVal: 4.467 ± 0.074	0.403GluTrp: 0.403 ± 0.023	2.99GluTyr: 2.99 ± 0.062	0.0GluXaa: 0.0 ± 0.0
Phe	2.152PheAla: 2.152 ± 0.059	0.545PheCys: 0.545 ± 0.027	2.306PheAsp: 2.306 ± 0.053	2.368PheGlu: 2.368 ± 0.06	1.94PhePhe: 1.94 ± 0.056	2.813PheGly: 2.813 ± 0.053	0.619PheHis: 0.619 ± 0.029	4.904PheIle: 4.904 ± 0.108	3.888PheLys: 3.888 ± 0.071	3.931PheLeu: 3.931 ± 0.078	1.166PheMet: 1.166 ± 0.037	2.951PheAsn: 2.951 ± 0.064	1.298PhePro: 1.298 ± 0.041	1.054PheGln: 1.054 ± 0.038	1.304PheArg: 1.304 ± 0.037	2.948PheSer: 2.948 ± 0.065	2.276PheThr: 2.276 ± 0.059	2.532PheVal: 2.532 ± 0.051	0.32PheTrp: 0.32 ± 0.02	1.854PheTyr: 1.854 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	4.147GlyAla: 4.147 ± 0.083	0.935GlyCys: 0.935 ± 0.037	3.338GlyAsp: 3.338 ± 0.072	4.794GlyGlu: 4.794 ± 0.082	3.1GlyPhe: 3.1 ± 0.06	4.315GlyGly: 4.315 ± 0.094	1.016GlyHis: 1.016 ± 0.037	6.885GlyIle: 6.885 ± 0.107	6.295GlyLys: 6.295 ± 0.101	5.332GlyLeu: 5.332 ± 0.094	1.853GlyMet: 1.853 ± 0.055	3.408GlyAsn: 3.408 ± 0.077	1.239GlyPro: 1.239 ± 0.041	1.403GlyGln: 1.403 ± 0.047	2.487GlyArg: 2.487 ± 0.061	3.597GlySer: 3.597 ± 0.078	3.556GlyThr: 3.556 ± 0.081	4.613GlyVal: 4.613 ± 0.082	0.463GlyTrp: 0.463 ± 0.026	3.009GlyTyr: 3.009 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	0.649HisAla: 0.649 ± 0.031	0.207HisCys: 0.207 ± 0.017	0.662HisAsp: 0.662 ± 0.025	0.836HisGlu: 0.836 ± 0.031	0.607HisPhe: 0.607 ± 0.026	1.099HisGly: 1.099 ± 0.037	0.24HisHis: 0.24 ± 0.016	1.443HisIle: 1.443 ± 0.049	1.082HisLys: 1.082 ± 0.038	1.11HisLeu: 1.11 ± 0.04	0.404HisMet: 0.404 ± 0.026	0.839HisAsn: 0.839 ± 0.031	0.671HisPro: 0.671 ± 0.03	0.332HisGln: 0.332 ± 0.019	0.589HisArg: 0.589 ± 0.024	0.86HisSer: 0.86 ± 0.036	0.636HisThr: 0.636 ± 0.029	0.799HisVal: 0.799 ± 0.031	0.095HisTrp: 0.095 ± 0.011	0.573HisTyr: 0.573 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.814IleAla: 5.814 ± 0.089	1.346IleCys: 1.346 ± 0.049	5.996IleAsp: 5.996 ± 0.084	7.076IleGlu: 7.076 ± 0.1	4.262IlePhe: 4.262 ± 0.086	6.476IleGly: 6.476 ± 0.102	1.42IleHis: 1.42 ± 0.044	9.776IleIle: 9.776 ± 0.158	9.159IleLys: 9.159 ± 0.127	9.081IleLeu: 9.081 ± 0.137	2.484IleMet: 2.484 ± 0.061	6.313IleAsn: 6.313 ± 0.096	3.656IlePro: 3.656 ± 0.073	2.333IleGln: 2.333 ± 0.05	3.174IleArg: 3.174 ± 0.06	6.492IleSer: 6.492 ± 0.106	4.919IleThr: 4.919 ± 0.073	5.986IleVal: 5.986 ± 0.085	0.566IleTrp: 0.566 ± 0.026	3.772IleTyr: 3.772 ± 0.07	0.0IleXaa: 0.0 ± 0.0
Lys	5.287LysAla: 5.287 ± 0.096	0.917LysCys: 0.917 ± 0.034	6.495LysAsp: 6.495 ± 0.113	9.31LysGlu: 9.31 ± 0.141	3.198LysPhe: 3.198 ± 0.074	5.876LysGly: 5.876 ± 0.078	1.135LysHis: 1.135 ± 0.034	8.474LysIle: 8.474 ± 0.103	8.58LysLys: 8.58 ± 0.115	7.848LysLeu: 7.848 ± 0.099	2.359LysMet: 2.359 ± 0.059	6.719LysAsn: 6.719 ± 0.111	2.217LysPro: 2.217 ± 0.056	2.128LysGln: 2.128 ± 0.053	3.199LysArg: 3.199 ± 0.061	5.124LysSer: 5.124 ± 0.086	3.954LysThr: 3.954 ± 0.071	6.113LysVal: 6.113 ± 0.087	0.635LysTrp: 0.635 ± 0.028	4.193LysTyr: 4.193 ± 0.07	0.0LysXaa: 0.0 ± 0.0
Leu	5.03LeuAla: 5.03 ± 0.08	1.173LeuCys: 1.173 ± 0.04	4.803LeuAsp: 4.803 ± 0.086	5.969LeuGlu: 5.969 ± 0.093	3.712LeuPhe: 3.712 ± 0.086	6.09LeuGly: 6.09 ± 0.107	1.15LeuHis: 1.15 ± 0.036	8.14LeuIle: 8.14 ± 0.125	8.86LeuLys: 8.86 ± 0.106	7.749LeuLeu: 7.749 ± 0.113	2.408LeuMet: 2.408 ± 0.059	5.963LeuAsn: 5.963 ± 0.092	3.014LeuPro: 3.014 ± 0.064	2.443LeuGln: 2.443 ± 0.053	3.114LeuArg: 3.114 ± 0.062	6.278LeuSer: 6.278 ± 0.094	4.333LeuThr: 4.333 ± 0.082	5.066LeuVal: 5.066 ± 0.083	0.578LeuTrp: 0.578 ± 0.024	3.35LeuTyr: 3.35 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	1.979MetAla: 1.979 ± 0.054	0.29MetCys: 0.29 ± 0.019	1.878MetAsp: 1.878 ± 0.05	2.184MetGlu: 2.184 ± 0.052	1.007MetPhe: 1.007 ± 0.036	1.994MetGly: 1.994 ± 0.048	0.374MetHis: 0.374 ± 0.02	2.103MetIle: 2.103 ± 0.051	2.622MetLys: 2.622 ± 0.057	2.314MetLeu: 2.314 ± 0.054	0.671MetMet: 0.671 ± 0.03	1.629MetAsn: 1.629 ± 0.047	0.956MetPro: 0.956 ± 0.037	0.685MetGln: 0.685 ± 0.032	0.898MetArg: 0.898 ± 0.033	1.679MetSer: 1.679 ± 0.046	1.203MetThr: 1.203 ± 0.042	1.752MetVal: 1.752 ± 0.051	0.153MetTrp: 0.153 ± 0.014	0.959MetTyr: 0.959 ± 0.039	0.0MetXaa: 0.0 ± 0.0
Asn	2.919AsnAla: 2.919 ± 0.06	0.804AsnCys: 0.804 ± 0.032	2.776AsnAsp: 2.776 ± 0.066	4.102AsnGlu: 4.102 ± 0.072	2.638AsnPhe: 2.638 ± 0.051	3.718AsnGly: 3.718 ± 0.078	0.817AsnHis: 0.817 ± 0.036	7.441AsnIle: 7.441 ± 0.111	6.396AsnLys: 6.396 ± 0.11	5.44AsnLeu: 5.44 ± 0.084	1.804AsnMet: 1.804 ± 0.044	4.473AsnAsn: 4.473 ± 0.099	2.123AsnPro: 2.123 ± 0.053	1.248AsnGln: 1.248 ± 0.043	2.105AsnArg: 2.105 ± 0.052	3.965AsnSer: 3.965 ± 0.077	2.907AsnThr: 2.907 ± 0.057	3.606AsnVal: 3.606 ± 0.064	0.462AsnTrp: 0.462 ± 0.026	2.727AsnTyr: 2.727 ± 0.063	0.0AsnXaa: 0.0 ± 0.0
Pro	1.568ProAla: 1.568 ± 0.045	0.386ProCys: 0.386 ± 0.019	1.506ProAsp: 1.506 ± 0.049	2.301ProGlu: 2.301 ± 0.05	1.443ProPhe: 1.443 ± 0.041	1.97ProGly: 1.97 ± 0.049	0.483ProHis: 0.483 ± 0.027	2.82ProIle: 2.82 ± 0.062	2.421ProLys: 2.421 ± 0.06	2.645ProLeu: 2.645 ± 0.059	0.849ProMet: 0.849 ± 0.038	1.563ProAsn: 1.563 ± 0.049	0.732ProPro: 0.732 ± 0.031	0.832ProGln: 0.832 ± 0.039	0.863ProArg: 0.863 ± 0.034	1.749ProSer: 1.749 ± 0.045	1.471ProThr: 1.471 ± 0.042	2.124ProVal: 2.124 ± 0.057	0.256ProTrp: 0.256 ± 0.016	1.435ProTyr: 1.435 ± 0.051	0.0ProXaa: 0.0 ± 0.0
Gln	1.387GlnAla: 1.387 ± 0.044	0.37GlnCys: 0.37 ± 0.021	1.195GlnAsp: 1.195 ± 0.037	1.563GlnGlu: 1.563 ± 0.044	0.934GlnPhe: 0.934 ± 0.033	1.647GlnGly: 1.647 ± 0.04	0.349GlnHis: 0.349 ± 0.021	2.067GlnIle: 2.067 ± 0.054	1.993GlnLys: 1.993 ± 0.049	2.163GlnLeu: 2.163 ± 0.057	0.718GlnMet: 0.718 ± 0.031	1.348GlnAsn: 1.348 ± 0.037	0.69GlnPro: 0.69 ± 0.036	0.805GlnGln: 0.805 ± 0.037	0.958GlnArg: 0.958 ± 0.036	1.331GlnSer: 1.331 ± 0.04	0.994GlnThr: 0.994 ± 0.042	1.527GlnVal: 1.527 ± 0.039	0.243GlnTrp: 0.243 ± 0.016	1.071GlnTyr: 1.071 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	1.952ArgAla: 1.952 ± 0.048	0.439ArgCys: 0.439 ± 0.025	1.927ArgAsp: 1.927 ± 0.049	2.951ArgGlu: 2.951 ± 0.068	1.521ArgPhe: 1.521 ± 0.043	2.11ArgGly: 2.11 ± 0.06	0.489ArgHis: 0.489 ± 0.025	3.361ArgIle: 3.361 ± 0.064	3.284ArgLys: 3.284 ± 0.067	3.017ArgLeu: 3.017 ± 0.058	1.027ArgMet: 1.027 ± 0.036	2.045ArgAsn: 2.045 ± 0.047	0.903ArgPro: 0.903 ± 0.035	0.958ArgGln: 0.958 ± 0.04	1.505ArgArg: 1.505 ± 0.048	1.556ArgSer: 1.556 ± 0.045	1.534ArgThr: 1.534 ± 0.042	2.265ArgVal: 2.265 ± 0.056	0.302ArgTrp: 0.302 ± 0.021	1.487ArgTyr: 1.487 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	3.148SerAla: 3.148 ± 0.068	0.736SerCys: 0.736 ± 0.032	2.713SerAsp: 2.713 ± 0.061	3.664SerGlu: 3.664 ± 0.066	2.959SerPhe: 2.959 ± 0.058	4.179SerGly: 4.179 ± 0.082	0.905SerHis: 0.905 ± 0.034	6.618SerIle: 6.618 ± 0.089	5.5SerLys: 5.5 ± 0.09	5.833SerLeu: 5.833 ± 0.101	1.748SerMet: 1.748 ± 0.058	3.515SerAsn: 3.515 ± 0.076	1.742SerPro: 1.742 ± 0.049	1.521SerGln: 1.521 ± 0.05	2.022SerArg: 2.022 ± 0.051	4.006SerSer: 4.006 ± 0.076	2.936SerThr: 2.936 ± 0.056	3.603SerVal: 3.603 ± 0.072	0.394SerTrp: 0.394 ± 0.023	2.531SerTyr: 2.531 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	3.111ThrAla: 3.111 ± 0.064	0.5ThrCys: 0.5 ± 0.028	2.373ThrAsp: 2.373 ± 0.066	2.874ThrGlu: 2.874 ± 0.052	2.116ThrPhe: 2.116 ± 0.051	3.668ThrGly: 3.668 ± 0.071	0.679ThrHis: 0.679 ± 0.028	4.941ThrIle: 4.941 ± 0.078	3.572ThrLys: 3.572 ± 0.068	4.613ThrLeu: 4.613 ± 0.082	1.238ThrMet: 1.238 ± 0.037	2.398ThrAsn: 2.398 ± 0.064	1.839ThrPro: 1.839 ± 0.049	1.006ThrGln: 1.006 ± 0.035	1.503ThrArg: 1.503 ± 0.047	3.001ThrSer: 3.001 ± 0.064	2.605ThrThr: 2.605 ± 0.061	3.304ThrVal: 3.304 ± 0.073	0.316ThrTrp: 0.316 ± 0.021	1.72ThrTyr: 1.72 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	3.744ValAla: 3.744 ± 0.069	0.863ValCys: 0.863 ± 0.032	3.728ValAsp: 3.728 ± 0.063	4.376ValGlu: 4.376 ± 0.078	2.788ValPhe: 2.788 ± 0.064	4.155ValGly: 4.155 ± 0.074	0.828ValHis: 0.828 ± 0.033	6.084ValIle: 6.084 ± 0.096	5.58ValLys: 5.58 ± 0.095	5.653ValLeu: 5.653 ± 0.092	1.684ValMet: 1.684 ± 0.046	3.745ValAsn: 3.745 ± 0.073	2.109ValPro: 2.109 ± 0.058	1.491ValGln: 1.491 ± 0.042	2.003ValArg: 2.003 ± 0.049	4.059ValSer: 4.059 ± 0.074	3.165ValThr: 3.165 ± 0.069	4.275ValVal: 4.275 ± 0.083	0.412ValTrp: 0.412 ± 0.023	2.389ValTyr: 2.389 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.367TrpAla: 0.367 ± 0.024	0.101TrpCys: 0.101 ± 0.013	0.39TrpAsp: 0.39 ± 0.021	0.43TrpGlu: 0.43 ± 0.025	0.297TrpPhe: 0.297 ± 0.02	0.474TrpGly: 0.474 ± 0.025	0.126TrpHis: 0.126 ± 0.014	0.661TrpIle: 0.661 ± 0.026	0.542TrpLys: 0.542 ± 0.028	0.552TrpLeu: 0.552 ± 0.026	0.201TrpMet: 0.201 ± 0.018	0.457TrpAsn: 0.457 ± 0.027	0.176TrpPro: 0.176 ± 0.016	0.227TrpGln: 0.227 ± 0.015	0.275TrpArg: 0.275 ± 0.017	0.398TrpSer: 0.398 ± 0.026	0.292TrpThr: 0.292 ± 0.018	0.411TrpVal: 0.411 ± 0.022	0.071TrpTrp: 0.071 ± 0.011	0.291TrpTyr: 0.291 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.065TyrAla: 2.065 ± 0.051	0.591TyrCys: 0.591 ± 0.028	2.309TyrAsp: 2.309 ± 0.059	2.861TyrGlu: 2.861 ± 0.06	2.0TyrPhe: 2.0 ± 0.048	2.712TyrGly: 2.712 ± 0.065	0.559TyrHis: 0.559 ± 0.028	4.153TyrIle: 4.153 ± 0.079	3.989TyrLys: 3.989 ± 0.078	3.536TyrLeu: 3.536 ± 0.065	1.048TyrMet: 1.048 ± 0.031	2.969TyrAsn: 2.969 ± 0.068	1.282TyrPro: 1.282 ± 0.046	0.743TyrGln: 0.743 ± 0.035	1.57TyrArg: 1.57 ± 0.047	2.785TyrSer: 2.785 ± 0.06	1.974TyrThr: 1.974 ± 0.042	2.335TyrVal: 2.335 ± 0.056	0.278TyrTrp: 0.278 ± 0.02	1.908TyrTyr: 1.908 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2785 proteins (831833 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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