Halapricum salinum

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Halapricum

Average proteome isoelectric point is 4.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3303 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6HIR9|A0A4D6HIR9_9EURY 2 5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one reductase OS=Halapricum salinum OX=1457250 GN=DV733_15775 PE=3 SV=1
MM1 pKa = 7.95PLTPGDD7 pKa = 4.1DD8 pKa = 3.82APTFAAQNQHH18 pKa = 6.25DD19 pKa = 4.25KK20 pKa = 11.14RR21 pKa = 11.84VVCQYY26 pKa = 11.08DD27 pKa = 3.62RR28 pKa = 11.84PTVVYY33 pKa = 7.9FYY35 pKa = 10.53PRR37 pKa = 11.84DD38 pKa = 3.79DD39 pKa = 3.98TPGCTIEE46 pKa = 5.8AEE48 pKa = 4.3GFDD51 pKa = 4.9DD52 pKa = 4.38SLSAYY57 pKa = 9.92RR58 pKa = 11.84EE59 pKa = 4.17AGVTVYY65 pKa = 10.47GVSTDD70 pKa = 3.33DD71 pKa = 3.83VEE73 pKa = 4.52SHH75 pKa = 7.01RR76 pKa = 11.84EE77 pKa = 3.58FADD80 pKa = 3.89EE81 pKa = 3.86YY82 pKa = 11.41DD83 pKa = 3.29IAFDD87 pKa = 5.36LLADD91 pKa = 4.22PDD93 pKa = 4.33GNIADD98 pKa = 4.09SFDD101 pKa = 3.61VPVEE105 pKa = 3.87NGAAARR111 pKa = 11.84TTFVVVDD118 pKa = 4.03GQVVATYY125 pKa = 10.43EE126 pKa = 4.36GVHH129 pKa = 6.47PEE131 pKa = 3.57GHH133 pKa = 6.33AADD136 pKa = 3.73VLEE139 pKa = 4.97DD140 pKa = 3.72LVEE143 pKa = 4.16TGIVSPP149 pKa = 4.86

Molecular weight: 16.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6HBX2|A0A4D6HBX2_9EURY Ribose-5-phosphate isomerase A OS=Halapricum salinum OX=1457250 GN=rpiA PE=3 SV=1
MM1 pKa = 6.88YY2 pKa = 10.32LRR4 pKa = 11.84RR5 pKa = 11.84IEE7 pKa = 4.22EE8 pKa = 4.31LPLEE12 pKa = 4.7DD13 pKa = 5.6RR14 pKa = 11.84LDD16 pKa = 3.8APADD20 pKa = 3.83RR21 pKa = 11.84KK22 pKa = 10.5ADD24 pKa = 3.88RR25 pKa = 11.84EE26 pKa = 4.6TPFLLGTTFSASGFLARR43 pKa = 11.84FVEE46 pKa = 4.86RR47 pKa = 11.84AAGTALRR54 pKa = 11.84LRR56 pKa = 11.84RR57 pKa = 11.84NGIRR61 pKa = 11.84SRR63 pKa = 11.84ASPATRR69 pKa = 11.84GAKK72 pKa = 8.27TWGKK76 pKa = 10.17KK77 pKa = 9.47PEE79 pKa = 4.36SPWEE83 pKa = 4.21GDD85 pKa = 3.34SGEE88 pKa = 4.29TATLSPYY95 pKa = 10.66AGQLYY100 pKa = 9.84SSRR103 pKa = 11.84PALPRR108 pKa = 11.84VARR111 pKa = 11.84SRR113 pKa = 11.84VTAFPVRR120 pKa = 11.84NRR122 pKa = 11.84DD123 pKa = 3.47APCGRR128 pKa = 11.84VSHH131 pKa = 7.01ALPATAGSRR140 pKa = 11.84TCRR143 pKa = 11.84GHH145 pKa = 5.74TLASGVTFKK154 pKa = 10.96SLRR157 pKa = 11.84SPVRR161 pKa = 11.84DD162 pKa = 3.39VSEE165 pKa = 4.54TPSPAPFLPRR175 pKa = 11.84TAAFLCDD182 pKa = 3.54QVVVLVLVVGPLAVAGVDD200 pKa = 3.38VLAPANRR207 pKa = 11.84TPIFLALMGAAFTYY221 pKa = 10.49HH222 pKa = 7.04FLLEE226 pKa = 4.25WLFGTTLGKK235 pKa = 10.31RR236 pKa = 11.84GFDD239 pKa = 3.46LRR241 pKa = 11.84VVADD245 pKa = 4.34DD246 pKa = 4.27GQPLGLWGSFVRR258 pKa = 11.84NALRR262 pKa = 11.84LIDD265 pKa = 4.32GLGYY269 pKa = 7.86WTVATVILFYY279 pKa = 10.91RR280 pKa = 11.84GDD282 pKa = 3.79GKK284 pKa = 11.12RR285 pKa = 11.84LGDD288 pKa = 3.46VLGRR292 pKa = 11.84TLVVRR297 pKa = 11.84ATTDD301 pKa = 2.75ARR303 pKa = 3.77

Molecular weight: 32.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3303

0

3303

1007341

30

3572

305.0

33.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.407 ± 0.062

0.695 ± 0.012

8.359 ± 0.054

8.614 ± 0.065

3.324 ± 0.025

8.411 ± 0.047

1.988 ± 0.021

4.288 ± 0.03

1.847 ± 0.026

8.974 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.714 ± 0.016

2.355 ± 0.034

4.616 ± 0.028

2.904 ± 0.026

6.397 ± 0.043

5.7 ± 0.041

6.542 ± 0.043

8.922 ± 0.051

1.191 ± 0.018

2.753 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski