Haloplasma contractile SSD-17B

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Haloplasmatales; Haloplasmataceae; Haloplasma; Haloplasma contractile

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7PU84|F7PU84_9MOLU Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Haloplasma contractile SSD-17B OX=1033810 GN=HLPCO_002213 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.24KK3 pKa = 9.38ILSFVFVVTLLAVLGVTSKK22 pKa = 11.64AEE24 pKa = 4.27VIDD27 pKa = 4.41SSTTADD33 pKa = 3.48NVLHH37 pKa = 6.31MKK39 pKa = 10.62DD40 pKa = 3.78DD41 pKa = 4.11AANDD45 pKa = 3.74AVYY48 pKa = 9.9GWSGLKK54 pKa = 9.96IYY56 pKa = 9.96WGTSDD61 pKa = 4.94NDD63 pKa = 3.59PANDD67 pKa = 3.16YY68 pKa = 10.89KK69 pKa = 11.17YY70 pKa = 10.52AYY72 pKa = 9.27KK73 pKa = 10.18VAKK76 pKa = 10.34GNDD79 pKa = 3.61YY80 pKa = 10.98QVDD83 pKa = 3.71QLDD86 pKa = 3.5QDD88 pKa = 3.66GDD90 pKa = 4.03NVVDD94 pKa = 4.55SLPSPKK100 pKa = 9.31WGSFGQVYY108 pKa = 9.97FNFSDD113 pKa = 3.55TDD115 pKa = 3.5IEE117 pKa = 4.26IPRR120 pKa = 11.84ALIGGFMILTVDD132 pKa = 3.33GDD134 pKa = 4.04GNAVRR139 pKa = 11.84FEE141 pKa = 4.38DD142 pKa = 4.48AYY144 pKa = 9.51KK145 pKa = 9.3THH147 pKa = 7.64DD148 pKa = 4.39LGEE151 pKa = 4.7CYY153 pKa = 9.78ATDD156 pKa = 4.04GSIVTYY162 pKa = 9.99TDD164 pKa = 3.0EE165 pKa = 5.24ATDD168 pKa = 3.81CRR170 pKa = 11.84QKK172 pKa = 10.89EE173 pKa = 4.29GNAQAIDD180 pKa = 3.38ASGNLLYY187 pKa = 10.81VDD189 pKa = 4.29ADD191 pKa = 3.91GNEE194 pKa = 4.37TTDD197 pKa = 3.39VTDD200 pKa = 3.41TPKK203 pKa = 10.88AKK205 pKa = 10.15KK206 pKa = 9.77DD207 pKa = 3.66ANDD210 pKa = 3.55EE211 pKa = 4.26NVTGLAWEE219 pKa = 4.4STTDD223 pKa = 3.28GDD225 pKa = 3.93VVYY228 pKa = 10.5IATEE232 pKa = 4.21DD233 pKa = 3.89YY234 pKa = 10.1NTFLTANPTYY244 pKa = 9.95TVVVYY249 pKa = 9.69DD250 pKa = 4.53IIVSNAVAMHH260 pKa = 7.28DD261 pKa = 3.44GTTFTRR267 pKa = 11.84QYY269 pKa = 10.92IDD271 pKa = 3.66FLGNPTDD278 pKa = 3.81NVVVPAGGFVLEE290 pKa = 4.9FEE292 pKa = 4.44WLDD295 pKa = 3.4RR296 pKa = 11.84GHH298 pKa = 6.86FANTNPYY305 pKa = 9.47IQTLFDD311 pKa = 3.47EE312 pKa = 4.78VVNYY316 pKa = 10.91NKK318 pKa = 9.97INYY321 pKa = 9.49SYY323 pKa = 9.91EE324 pKa = 3.81ATVAPTISGVTDD336 pKa = 3.28VNVEE340 pKa = 4.33LGQIKK345 pKa = 10.53SNWLEE350 pKa = 3.98DD351 pKa = 3.81VNASTSQYY359 pKa = 11.04NYY361 pKa = 8.62NTGAMTTIDD370 pKa = 3.54GSVTCTVVEE379 pKa = 4.13SDD381 pKa = 3.42LTEE384 pKa = 4.09VGPCNTTNIDD394 pKa = 3.89TNVMDD399 pKa = 4.44AQYY402 pKa = 11.23KK403 pKa = 9.15LAYY406 pKa = 10.17SVTDD410 pKa = 3.78DD411 pKa = 4.15AGLEE415 pKa = 4.23SVVYY419 pKa = 9.19STVTVVDD426 pKa = 4.02GLAPVISGVDD436 pKa = 3.48NIIVDD441 pKa = 4.15EE442 pKa = 4.63GTDD445 pKa = 3.78LTDD448 pKa = 4.62LLLDD452 pKa = 5.74GITADD457 pKa = 4.53DD458 pKa = 5.26GYY460 pKa = 11.73GNDD463 pKa = 3.01ISEE466 pKa = 5.13NINIVHH472 pKa = 7.61GITDD476 pKa = 3.47VTNPKK481 pKa = 10.32AGTYY485 pKa = 7.48TVTYY489 pKa = 8.95FVYY492 pKa = 9.99NHH494 pKa = 6.39KK495 pKa = 10.53VKK497 pKa = 10.9NDD499 pKa = 3.28FASAVITVNDD509 pKa = 3.48VTNPTVVAGDD519 pKa = 3.2QHH521 pKa = 7.86IDD523 pKa = 3.06QGQEE527 pKa = 3.32FDD529 pKa = 4.09LKK531 pKa = 11.19SGILYY536 pKa = 9.73MSDD539 pKa = 4.22NIDD542 pKa = 3.26NTLDD546 pKa = 3.71VVVTDD551 pKa = 5.5DD552 pKa = 4.79GGFDD556 pKa = 3.72KK557 pKa = 10.55DD558 pKa = 3.57TAGEE562 pKa = 4.13YY563 pKa = 9.32TVAIDD568 pKa = 5.38VYY570 pKa = 10.61DD571 pKa = 3.54QSGNKK576 pKa = 9.98ASTTYY581 pKa = 10.58KK582 pKa = 8.38VTVVAPVEE590 pKa = 4.0IPEE593 pKa = 4.12IPEE596 pKa = 3.66IPEE599 pKa = 3.67IPEE602 pKa = 3.72IPEE605 pKa = 3.87IPEE608 pKa = 4.48DD609 pKa = 3.82SSGLATSGLVVGIVALIVAAGGFVISRR636 pKa = 11.84KK637 pKa = 8.26FF638 pKa = 3.09

Molecular weight: 68.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7PSY9|F7PSY9_9MOLU 50S ribosomal protein L31 OS=Haloplasma contractile SSD-17B OX=1033810 GN=rpmE PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 9.47RR3 pKa = 11.84TWQPNKK9 pKa = 9.6RR10 pKa = 11.84KK11 pKa = 9.84RR12 pKa = 11.84NKK14 pKa = 8.11THH16 pKa = 6.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPTGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.41KK37 pKa = 10.29GRR39 pKa = 11.84KK40 pKa = 8.9KK41 pKa = 10.19LTVV44 pKa = 3.1

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3019

0

3019

927687

24

2346

307.3

35.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.062 ± 0.047

0.847 ± 0.017

6.045 ± 0.044

7.14 ± 0.055

4.658 ± 0.04

5.95 ± 0.048

2.041 ± 0.022

9.228 ± 0.05

7.685 ± 0.056

9.626 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.021

5.994 ± 0.044

2.845 ± 0.027

3.03 ± 0.027

3.677 ± 0.029

5.964 ± 0.033

5.726 ± 0.054

6.603 ± 0.039

0.73 ± 0.015

4.714 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski